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UniProtKB - O25991 (MNME_HELPY)
Protein
tRNA modification GTPase MnmE
Gene
mnmE
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34.
UniRule annotationCofactori
K+UniRule annotationNote: Binds 1 potassium ion per subunit.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 32 | FormyltetrahydrofolateUniRule annotation | 1 | |
Binding sitei | 89 | FormyltetrahydrofolateUniRule annotation | 1 | |
Binding sitei | 128 | FormyltetrahydrofolateUniRule annotation | 1 | |
Metal bindingi | 234 | PotassiumUniRule annotation | 1 | |
Metal bindingi | 238 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 253 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 255 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 258 | PotassiumUniRule annotation | 1 | |
Metal bindingi | 259 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 461 | FormyltetrahydrofolateUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 234 – 239 | GTPUniRule annotation | 6 | |
Nucleotide bindingi | 253 – 259 | GTPUniRule annotation | 7 | |
Nucleotide bindingi | 278 – 281 | GTPUniRule annotation | 4 |
GO - Molecular functioni
- GTPase activity Source: UniProtKB-UniRule
- GTP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- tRNA methylation Source: GO_Central
- tRNA wobble uridine modification Source: GO_Central
Keywordsi
Molecular function | Hydrolase |
Biological process | tRNA processing |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:mnmEUniRule annotation Synonyms:thdFUniRule annotation, trmEUniRule annotation Ordered Locus Names:HP_1452 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188882 | 1 – 461 | tRNA modification GTPase MnmEAdd BLAST | 461 |
Proteomic databases
PaxDbi | O25991 |
Interactioni
Subunit structurei
Homodimer. Heterotetramer of two MnmE and two MnmG subunits.
UniRule annotationProtein-protein interaction databases
DIPi | DIP-3365N |
IntActi | O25991, 3 interactors |
MINTi | O25991 |
STRINGi | 85962.C694_07520 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 224 – 387 | TrmE-type GAdd BLAST | 164 |
Sequence similaritiesi
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. TrmE GTPase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0486, Bacteria |
OMAi | EFHCHGG |
PhylomeDBi | O25991 |
Family and domain databases
CDDi | cd04164, trmE, 1 hit |
Gene3Di | 1.20.120.430, 1 hit 3.30.1360.120, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00379, GTPase_MnmE, 1 hit |
InterProi | View protein in InterPro IPR031168, G_TrmE IPR006073, GTP-bd IPR018948, GTP-bd_TrmE_N IPR004520, GTPase_MnmE IPR027368, MnmE_dom2 IPR025867, MnmE_helical IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR027266, TrmE/GcvT_dom1 |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit PF12631, MnmE_helical, 1 hit PF10396, TrmE_N, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00450, mnmE_trmE_thdF, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51709, G_TRME, 1 hit |
i Sequence
Sequence statusi: Complete.
O25991-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKNTSSSTTL TMNDTIAAIA TPLGKGAISI IKISGHNALN ILKQLTQKQD
60 70 80 90 100
FTPRYAYVHD IFSNGVLLDK ALVIYFKAPY SFTGEDVCEI QCHGSPLLAQ
110 120 130 140 150
NILQACLNLG ARLAKAGEFS KKAFLNHKMD LSEIEASVQL ILCEDESVLN
160 170 180 190 200
ALARQLKGEL KIFIEEARGN LLKLLASSEV LIDYSEEDIP SDFLDGVSLN
210 220 230 240 250
LEKQIASFKD LLDFSNAQKQ RNKGHALSIV GKPNAGKSSL LNAMLLEERA
260 270 280 290 300
LVSDIKGTTR DTIEEVIELK GHKVRLIDTA GIRESADKIE RLGIEKSLKS
310 320 330 340 350
LENCDIILGV FDLSKPLEKE DFNLIDTLNR AKKPCIVVLN KNDLAPKLEL
360 370 380 390 400
EILKSYLKIP YTLLETNTLN SKACLKDLSQ KISAFFPKLD TQNKLLLTSL
410 420 430 440 450
AQKIALENAI TELQNAKNHL ETLELFSYHI LSAIENLNLL TRPYETSQML
460
DSMFSEFCLG K
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08492.1 |
PIRi | D64701 |
RefSeqi | NP_208243.1, NC_000915.1 WP_000799508.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD08492; AAD08492; HP_1452 |
KEGGi | hpy:HP_1452 |
PATRICi | fig|85962.8.peg.1524 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08492.1 |
PIRi | D64701 |
RefSeqi | NP_208243.1, NC_000915.1 WP_000799508.1, NC_018939.1 |
3D structure databases
SMRi | O25991 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-3365N |
IntActi | O25991, 3 interactors |
MINTi | O25991 |
STRINGi | 85962.C694_07520 |
Proteomic databases
PaxDbi | O25991 |
Genome annotation databases
EnsemblBacteriai | AAD08492; AAD08492; HP_1452 |
KEGGi | hpy:HP_1452 |
PATRICi | fig|85962.8.peg.1524 |
Phylogenomic databases
eggNOGi | COG0486, Bacteria |
OMAi | EFHCHGG |
PhylomeDBi | O25991 |
Family and domain databases
CDDi | cd04164, trmE, 1 hit |
Gene3Di | 1.20.120.430, 1 hit 3.30.1360.120, 1 hit 3.40.50.300, 1 hit |
HAMAPi | MF_00379, GTPase_MnmE, 1 hit |
InterProi | View protein in InterPro IPR031168, G_TrmE IPR006073, GTP-bd IPR018948, GTP-bd_TrmE_N IPR004520, GTPase_MnmE IPR027368, MnmE_dom2 IPR025867, MnmE_helical IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR027266, TrmE/GcvT_dom1 |
Pfami | View protein in Pfam PF01926, MMR_HSR1, 1 hit PF12631, MnmE_helical, 1 hit PF10396, TrmE_N, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00450, mnmE_trmE_thdF, 1 hit TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51709, G_TRME, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MNME_HELPY | |
Accessioni | O25991Primary (citable) accession number: O25991 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 124 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families