The new UniProt website is here!
Take me to UniProt BETA
UniProtKB - O25972 (RSMA_HELPY)
Protein
Ribosomal RNA small subunit methyltransferase A
Gene
rsmA
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.
UniRule annotationCatalytic activityi
- adenosine1518/adenosine1519 in 16S rRNA + 4 S-adenosyl-L-methionine = 4 H+ + N6-dimethyladenosine1518/N6-dimethyladenosine1519 in 16S rRNA + 4 S-adenosyl-L-homocysteineUniRule annotationEC:2.1.1.182UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 11 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 13 | S-adenosyl-L-methionine; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 38 | S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 58 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 86 | S-adenosyl-L-methionineUniRule annotation | 1 | |
Binding sitei | 101 | S-adenosyl-L-methionineUniRule annotation | 1 |
GO - Molecular functioni
- 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase activity Source: UniProtKB-EC
- RNA binding Source: UniProtKB-KW
- rRNA (adenine-N6,N6-)-dimethyltransferase activity Source: GO_Central
GO - Biological processi
- rRNA methylation Source: GO_Central
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | rRNA processing |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Ribosomal RNA small subunit methyltransferase AUniRule annotation (EC:2.1.1.182UniRule annotation)Alternative name(s): 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferaseUniRule annotation 16S rRNA dimethyladenosine transferaseUniRule annotation 16S rRNA dimethylaseUniRule annotation S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferaseUniRule annotation |
Gene namesi | Name:rsmAUniRule annotation Synonyms:ksgAUniRule annotation Ordered Locus Names:HP_1431 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101540 | 1 – 271 | Ribosomal RNA small subunit methyltransferase AAdd BLAST | 271 |
Proteomic databases
PaxDbi | O25972 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | COG0030, Bacteria |
OMAi | KEEEPYF |
PhylomeDBi | O25972 |
Family and domain databases
Gene3Di | 1.10.8.100, 1 hit 3.40.50.150, 1 hit |
HAMAPi | MF_00607, 16SrRNA_methyltr_A, 1 hit |
InterProi | View protein in InterPro IPR001737, KsgA/Erm IPR023165, rRNA_Ade_diMease-like_C IPR020596, rRNA_Ade_Mease_Trfase_CS IPR020598, rRNA_Ade_methylase_Trfase_N IPR011530, rRNA_adenine_dimethylase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11727, PTHR11727, 1 hit |
Pfami | View protein in Pfam PF00398, RrnaAD, 1 hit |
SMARTi | View protein in SMART SM00650, rADc, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00755, ksgA, 1 hit |
PROSITEi | View protein in PROSITE PS01131, RRNA_A_DIMETH, 1 hit PS51689, SAM_RNA_A_N6_MT, 1 hit |
i Sequence
Sequence statusi: Complete.
O25972-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVVAKKSLGQ HFLTDESFLD RIVNALPPLN PLKLVEIGVG LGDLTLKLLD
60 70 80 90 100
RYPLKTYEID SHLCEKMRSK LKAQKKPFKL ELVEKDALFL KEEEPYFLIS
110 120 130 140 150
NLPYYIATRL VLNAFKDPKC RGLLVMTQKE VALKFCAKDS QNALSVLAHT
160 170 180 190 200
IGNATLLFDV PPSAFSPPPK VFSSVFEVIK EPLKEKALAS LAQAPFFEEA
210 220 230 240 250
LQKGFEMLED FLKACFSSPR KTLSNNLKKS VSYREKLDKV LDFLALENQP
260 270
TSVRASEIKD YLKLLNYLLK G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08470.1 |
PIRi | G64698 |
RefSeqi | NP_208222.1, NC_000915.1 WP_000259453.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD08470; AAD08470; HP_1431 |
KEGGi | hpy:HP_1431 |
PATRICi | fig|85962.47.peg.1535 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08470.1 |
PIRi | G64698 |
RefSeqi | NP_208222.1, NC_000915.1 WP_000259453.1, NC_018939.1 |
3D structure databases
AlphaFoldDBi | O25972 |
SMRi | O25972 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 85962.C694_07400 |
Proteomic databases
PaxDbi | O25972 |
Genome annotation databases
EnsemblBacteriai | AAD08470; AAD08470; HP_1431 |
KEGGi | hpy:HP_1431 |
PATRICi | fig|85962.47.peg.1535 |
Phylogenomic databases
eggNOGi | COG0030, Bacteria |
OMAi | KEEEPYF |
PhylomeDBi | O25972 |
Family and domain databases
Gene3Di | 1.10.8.100, 1 hit 3.40.50.150, 1 hit |
HAMAPi | MF_00607, 16SrRNA_methyltr_A, 1 hit |
InterProi | View protein in InterPro IPR001737, KsgA/Erm IPR023165, rRNA_Ade_diMease-like_C IPR020596, rRNA_Ade_Mease_Trfase_CS IPR020598, rRNA_Ade_methylase_Trfase_N IPR011530, rRNA_adenine_dimethylase IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR11727, PTHR11727, 1 hit |
Pfami | View protein in Pfam PF00398, RrnaAD, 1 hit |
SMARTi | View protein in SMART SM00650, rADc, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
TIGRFAMsi | TIGR00755, ksgA, 1 hit |
PROSITEi | View protein in PROSITE PS01131, RRNA_A_DIMETH, 1 hit PS51689, SAM_RNA_A_N6_MT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RSMA_HELPY | |
Accessioni | O25972Primary (citable) accession number: O25972 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 13, 2002 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 116 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families