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UniProtKB - O25916 (DNAB_HELPY)
Protein
Replicative DNA helicase
Gene
dnaB
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with DnaC protein, primase, and other prepriming proteins.
By similarityCatalytic activityi
- EC:3.6.4.12
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 203 – 210 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RHEA
- DNA binding Source: UniProtKB-KW
- DNA helicase activity Source: GO_Central
- identical protein binding Source: IntAct
GO - Biological processi
- DNA replication, synthesis of RNA primer Source: UniProtKB-KW
- DNA unwinding involved in DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Helicase, Hydrolase |
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:dnaB Ordered Locus Names:HP_1362 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Other locations
- primosome complex Source: UniProtKB-KW
Keywords - Cellular componenti
PrimosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000102023 | 1 – 488 | Replicative DNA helicaseAdd BLAST | 488 |
Proteomic databases
PaxDbi | O25916 |
Interactioni
Binary interactionsi
O25916
With | #Exp. | IntAct |
---|---|---|
itself | 6 | EBI-7529552,EBI-7529552 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
DIPi | DIP-3388N |
IntActi | O25916, 4 interactors |
MINTi | O25916 |
STRINGi | 85962.C694_07030 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O25916 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O25916 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 172 – 471 | SF4 helicasePROSITE-ProRule annotationAdd BLAST | 300 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0305, Bacteria |
OMAi | WENLAKC |
PhylomeDBi | O25916 |
Family and domain databases
CDDi | cd00984, DnaB_C, 1 hit |
Gene3Di | 1.10.860.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR036185, DNA_heli_DnaB-like_N_sf IPR007692, DNA_helicase_DnaB IPR007694, DNA_helicase_DnaB-like_C IPR007693, DNA_helicase_DnaB-like_N IPR016136, DNA_helicase_N/primase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00772, DnaB, 1 hit PF03796, DnaB_C, 1 hit |
SUPFAMi | SSF48024, SSF48024, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00665, DnaB, 1 hit |
PROSITEi | View protein in PROSITE PS51199, SF4_HELICASE, 1 hit |
i Sequence
Sequence statusi: Complete.
O25916-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDHLKHLQQL QNIERIVLSG IVLANHKIEE VHSVLEPSDF YYPPNGLFFE
60 70 80 90 100
IALKLHEEDC PIDENFIRQK MPKDKQIKEE DLVAIFAASP IDNIEAYVEE
110 120 130 140 150
IKNASIKRKL FGLANTIREQ ALESAQKSSD ILGAVEREVY ALLNGSTIEG
160 170 180 190 200
FRNIKEVLES AMDLITENQR KGSLEVTGIP TGFVQLDNYT SGFNKGSLVI
210 220 230 240 250
IGARPSMGKT SLMMNMVLSA LNDDRGVAVF SLEMSAEQLA LRALSDLTSI
260 270 280 290 300
NMHDLESGRL DDDQWENLAK CFDHLSQKKL FFYDKSYVRI EQIRLQLRKL
310 320 330 340 350
KSQHKELGIA FIDYLQLMSG SKATKERHEQ IAEISRELKT LARELEIPII
360 370 380 390 400
ALVQLNRSLE NRDDKRPILS DIKDSGGIEQ DADIVLFLYR GYIYQMRAED
410 420 430 440 450
NKIDKLKKEG KIEEAQELYL KVNEERRIHK QNGSIEEAEI IVAKNRNGAT
460 470 480
GTVYTRFNAP FTRYEDMPID SHLEEGQETK VDYDIVTT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08402.1 |
PIRi | B64690 |
RefSeqi | NP_208154.1, NC_000915.1 WP_000349722.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD08402; AAD08402; HP_1362 |
KEGGi | hpy:HP_1362 |
PATRICi | fig|85962.47.peg.1459 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08402.1 |
PIRi | B64690 |
RefSeqi | NP_208154.1, NC_000915.1 WP_000349722.1, NC_018939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3GXV | X-ray | 2.20 | A/B | 1-121 | [»] | |
C | 101-122 | [»] | ||||
D | 98-123 | [»] | ||||
4A1F | X-ray | 2.50 | A/B | 152-488 | [»] | |
4ZC0 | X-ray | 6.70 | A/B/C/D | 1-488 | [»] | |
SMRi | O25916 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-3388N |
IntActi | O25916, 4 interactors |
MINTi | O25916 |
STRINGi | 85962.C694_07030 |
Proteomic databases
PaxDbi | O25916 |
Genome annotation databases
EnsemblBacteriai | AAD08402; AAD08402; HP_1362 |
KEGGi | hpy:HP_1362 |
PATRICi | fig|85962.47.peg.1459 |
Phylogenomic databases
eggNOGi | COG0305, Bacteria |
OMAi | WENLAKC |
PhylomeDBi | O25916 |
Miscellaneous databases
EvolutionaryTracei | O25916 |
Family and domain databases
CDDi | cd00984, DnaB_C, 1 hit |
Gene3Di | 1.10.860.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR036185, DNA_heli_DnaB-like_N_sf IPR007692, DNA_helicase_DnaB IPR007694, DNA_helicase_DnaB-like_C IPR007693, DNA_helicase_DnaB-like_N IPR016136, DNA_helicase_N/primase_C IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00772, DnaB, 1 hit PF03796, DnaB_C, 1 hit |
SUPFAMi | SSF48024, SSF48024, 1 hit SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00665, DnaB, 1 hit |
PROSITEi | View protein in PROSITE PS51199, SF4_HELICASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DNAB_HELPY | |
Accessioni | O25916Primary (citable) accession number: O25916 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families