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UniProtKB - O25655 (PYRD_HELPY)
Protein
Dihydroorotate dehydrogenase (quinone)
Gene
pyrD
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor.
By similarityCatalytic activityi
- EC:1.3.5.2
Cofactori
FMNBy similarityNote: Binds 1 FMN per subunit.By similarity
: UMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route). This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes orotate from (S)-dihydroorotate (quinone route), the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 71 | SubstrateBy similarity | 1 | |
Binding sitei | 91 | FMN; via amide nitrogenBy similarity | 1 | |
Binding sitei | 145 | FMNBy similarity | 1 | |
Binding sitei | 178 | FMNBy similarity | 1 | |
Binding sitei | 178 | SubstrateBy similarity | 1 | |
Active sitei | 181 | NucleophileBy similarity | 1 | |
Binding sitei | 183 | SubstrateBy similarity | 1 | |
Binding sitei | 214 | FMNBy similarity | 1 | |
Binding sitei | 242 | FMN; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 262 | FMN; via amide nitrogenBy similarity | 1 | |
Binding sitei | 291 | FMN; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 67 – 71 | FMNBy similarity | 5 | |
Nucleotide bindingi | 312 – 313 | FMNBy similarity | 2 |
GO - Molecular functioni
- dihydroorotate dehydrogenase activity Source: GO_Central
GO - Biological processi
- 'de novo' pyrimidine nucleobase biosynthetic process Source: GO_Central
- 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
- pyrimidine ribonucleotide biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Pyrimidine biosynthesis |
Ligand | Flavoprotein, FMN |
Enzyme and pathway databases
UniPathwayi | UPA00070;UER00946 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydroorotate dehydrogenase (quinone) (EC:1.3.5.2)Alternative name(s): DHOdehase Short name: DHOD Short name: DHODase Dihydroorotate oxidase |
Gene namesi | Name:pyrD Ordered Locus Names:HP_1011 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: InterPro
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000148445 | 1 – 351 | Dihydroorotate dehydrogenase (quinone)Add BLAST | 351 |
Proteomic databases
PaxDbi | O25655 |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 85962.C694_05235 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 116 – 120 | Substrate bindingBy similarity | 5 | |
Regioni | 243 – 244 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0167, Bacteria |
OMAi | LQNAMGF |
PhylomeDBi | O25655 |
Family and domain databases
CDDi | cd04738, DHOD_2_like, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00225, DHO_dh_type2, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR005720, Dihydroorotate_DH IPR012135, Dihydroorotate_DH_1_2 IPR005719, Dihydroorotate_DH_2 IPR001295, Dihydroorotate_DH_CS |
Pfami | View protein in Pfam PF01180, DHO_dh, 1 hit |
PIRSFi | PIRSF000164, DHO_oxidase, 1 hit |
TIGRFAMsi | TIGR01036, pyrD_sub2, 1 hit |
PROSITEi | View protein in PROSITE PS00911, DHODEHASE_1, 1 hit PS00912, DHODEHASE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O25655-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLYSLVKKYL FSLDAEDAHE KVCKILRMLS SSPFLCNLID SQWGYQNPKL
60 70 80 90 100
ENEILGLHFP NPLGLAAGFD KNASMLRALM AFGFGYLEAG TLTNEAQVGN
110 120 130 140 150
ERPRLFRHIE EESLQNAMGF NNYGAILGVR SFKRFAPYKT PIGINLGKNK
160 170 180 190 200
HIEQAHALED YKAVLSKCLN IGDYYTFNLS SPNTPNLRDL QNKAFVHELF
210 220 230 240 250
CMAKEMTHKP LFLKIAPDLE TDDMLEIVNS AIGAGAHGII ATNTTIDKSL
260 270 280 290 300
VFAPKEMGGL SGKCLTKKSR EIFKELAKAF FNKSVLVSVG GISDAKEAYE
310 320 330 340 350
RIKMGASLLQ IYSAFIYNGP NLCQNILKDL VKLLQKDGFL SVKEAIGADL
R
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08055.1 |
PIRi | C64646 |
RefSeqi | NP_207801.1, NC_000915.1 WP_000967190.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD08055; AAD08055; HP_1011 |
KEGGi | hpy:HP_1011 |
PATRICi | fig|85962.47.peg.1090 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD08055.1 |
PIRi | C64646 |
RefSeqi | NP_207801.1, NC_000915.1 WP_000967190.1, NC_018939.1 |
3D structure databases
SMRi | O25655 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 85962.C694_05235 |
Proteomic databases
PaxDbi | O25655 |
Genome annotation databases
EnsemblBacteriai | AAD08055; AAD08055; HP_1011 |
KEGGi | hpy:HP_1011 |
PATRICi | fig|85962.47.peg.1090 |
Phylogenomic databases
eggNOGi | COG0167, Bacteria |
OMAi | LQNAMGF |
PhylomeDBi | O25655 |
Enzyme and pathway databases
UniPathwayi | UPA00070;UER00946 |
Family and domain databases
CDDi | cd04738, DHOD_2_like, 1 hit |
Gene3Di | 3.20.20.70, 1 hit |
HAMAPi | MF_00225, DHO_dh_type2, 1 hit |
InterProi | View protein in InterPro IPR013785, Aldolase_TIM IPR005720, Dihydroorotate_DH IPR012135, Dihydroorotate_DH_1_2 IPR005719, Dihydroorotate_DH_2 IPR001295, Dihydroorotate_DH_CS |
Pfami | View protein in Pfam PF01180, DHO_dh, 1 hit |
PIRSFi | PIRSF000164, DHO_oxidase, 1 hit |
TIGRFAMsi | TIGR01036, pyrD_sub2, 1 hit |
PROSITEi | View protein in PROSITE PS00911, DHODEHASE_1, 1 hit PS00912, DHODEHASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PYRD_HELPY | |
Accessioni | O25655Primary (citable) accession number: O25655 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families