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UniProtKB - O25608 (RDXA_HELPY)

Entry version 91 (11 Dec 2019)
Sequence version 1 (01 Jan 1998)
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Protein

Oxygen-insensitive NADPH nitroreductase

Gene

rdxA

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Reduction of a variety of nitroaromatic compounds using NADPH as source of reducing equivalents; two electrons are transferred (By similarity). Capable of reducing metronidazole; inactive RdxA renders the bacterium resistant to this compound. The reduction of metronidazole generates hydroxylamine, a potent mutagen and bactericide.By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi150 – 155NADPBy similarity6

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionOxidoreductase
Biological processAntibiotic resistance
LigandNADP

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		HPY:HP0954-MONOMER

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Oxygen-insensitive NADPH nitroreductase (EC:1.-.-.-)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:rdxA
Ordered Locus Names:HP_0954
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri85962 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter ›
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000429 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi19C → Y: Loss of function and acquisition of resistance to metronidazole. 1 Publication1
Mutagenesisi49T → K: Loss of function and acquisition of resistance to metronidazole. 1 Publication1

Chemistry databases

Drug and drug target database

More...DrugBanki		DB00916 Metronidazole

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00003218641 – 210Oxygen-insensitive NADPH nitroreductaseAdd BLAST210

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O25608

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Database of interacting proteins

More...DIPi		DIP-3602N

Protein interaction database and analysis system

More...IntActi		O25608, 2 interactors

Molecular INTeraction database

More...MINTi		O25608

STRING: functional protein association networks

More...STRINGi		85962.C694_04915

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1210
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O25608

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the nitroreductase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG4108RCM Bacteria
COG0778 LUCA

Identification of Orthologs from Complete Genome Data

More...OMAi		MQNLYPE

Family and domain databases

Conserved Domains Database

More...CDDi		cd02149 NfsB_like_nitroreductase, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.109.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR033878 NfsB-like
IPR029479 Nitroreductase
IPR000415 Nitroreductase-like

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00881 Nitroreductase, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF55469 SSF55469, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O25608-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKFLDQEKRR QLLNERHSCK MFDSHYEFSS TELEEIAEIA RLSPSSYNTQ 
        60         70         80         90        100
PWHFVMVTDK DLKKQIAAHS YFNEEMIKSA SALMVVCSLR PSELLPHGHY 
       110        120        130        140        150
MQNLYPESYK VRVIPSFAQM LGVRFNHSMQ RLESYILEQC YIAVGQICMG 
       160        170        180        190        200
VSLMGLDSCI IGGFDPLKVG EVLEERINKP KIACLIALGK RVAEASQKSR 
       210
KSKVDAITWL
Length:210
Mass (Da):24,068
Last modified:January 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iDFF15D2D102C002C
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural varianti14N → K in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. 1
Natural varianti31T → E in strain: HP500, 69A and ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. 1
Natural varianti36I → V in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. 1
Natural varianti53H → R in strain: ATCC 49503. 1
Natural varianti56M → I in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. 1
Natural varianti59D → N in strain: HP500, 69A, ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole and ATCC 49503 / 60190. 1
Natural varianti63 – 93KKQIA…LRPSE → NHPSRNPKH in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. Add BLAST31
Natural varianti64K → N in strain: 69A. 1
Natural varianti68A → V in strain: HP500. 1
Natural varianti90R → K in strain: ATCC 49503 / 60190. 1
Natural varianti97H → T in strain: 69A. 1
Natural varianti98G → S in strain: ATCC 49503 / 60190. 1
Natural varianti106P → S in strain: 69A. 1
Natural varianti131R → K in strain: 69A. 1
Natural varianti172V → I in strain: 69A. 1
Natural varianti183A → V in strain: ATCC 49503 / 60190. 1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF012552 Genomic DNA Translation: AAC46349.1
AF315501 Genomic DNA Translation: AAK17124.1
AF315502 Genomic DNA Translation: AAK17125.1
AF316109 Genomic DNA Translation: AAK19260.1
AE000511 Genomic DNA Translation: AAD07997.1

Protein sequence database of the Protein Information Resource

More...PIRi		B64639

NCBI Reference Sequences

More...RefSeqi		NP_207746.1, NC_000915.1
WP_000670110.1, NC_018939.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...EnsemblBacteriai		AAD07997; AAD07997; HP_0954

Database of genes from NCBI RefSeq genomes

More...GeneIDi		899487

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		heo:C694_04915
hpy:HP0954

Pathosystems Resource Integration Center (PATRIC)

More...PATRICi		fig|85962.47.peg.1022

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF012552 Genomic DNA Translation: AAC46349.1
AF315501 Genomic DNA Translation: AAK17124.1
AF315502 Genomic DNA Translation: AAK17125.1
AF316109 Genomic DNA Translation: AAK19260.1
AE000511 Genomic DNA Translation: AAD07997.1
PIRiB64639
RefSeqiNP_207746.1, NC_000915.1
WP_000670110.1, NC_018939.1

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3QDLX-ray2.00A/B/C/D1-210[»]
SMRiO25608
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

DIPiDIP-3602N
IntActiO25608, 2 interactors
MINTiO25608
STRINGi85962.C694_04915

Chemistry databases

DrugBankiDB00916 Metronidazole

Proteomic databases

PaxDbiO25608

Genome annotation databases

EnsemblBacteriaiAAD07997; AAD07997; HP_0954
GeneIDi899487
KEGGiheo:C694_04915
hpy:HP0954
PATRICifig|85962.47.peg.1022

Phylogenomic databases

eggNOGiENOG4108RCM Bacteria
COG0778 LUCA
OMAiMQNLYPE

Enzyme and pathway databases

BioCyciHPY:HP0954-MONOMER

Family and domain databases

CDDicd02149 NfsB_like_nitroreductase, 1 hit
Gene3Di3.40.109.10, 1 hit
InterProiView protein in InterPro
IPR033878 NfsB-like
IPR029479 Nitroreductase
IPR000415 Nitroreductase-like
PfamiView protein in Pfam
PF00881 Nitroreductase, 1 hit
SUPFAMiSSF55469 SSF55469, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRDXA_HELPY
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O25608
Secondary accession number(s): O30585
, Q9AHD6, Q9AHF7, Q9AHF8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 1, 1998
Last modified: December 11, 2019
This is version 91 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
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