UniProtKB - O25608 (RDXA_HELPY)
Protein
Oxygen-insensitive NADPH nitroreductase
Gene
rdxA
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Reduction of a variety of nitroaromatic compounds using NADPH as source of reducing equivalents; two electrons are transferred (By similarity). Capable of reducing metronidazole; inactive RdxA renders the bacterium resistant to this compound. The reduction of metronidazole generates hydroxylamine, a potent mutagen and bactericide.By similarity1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 150 – 155 | NADPBy similarity | 6 |
GO - Molecular functioni
- oxidoreductase activity Source: UniProtKB-KW
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Antibiotic resistance |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | HPY:HP0954-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:rdxA Ordered Locus Names:HP_0954 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 19 | C → Y: Loss of function and acquisition of resistance to metronidazole. 1 Publication | 1 | |
Mutagenesisi | 49 | T → K: Loss of function and acquisition of resistance to metronidazole. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB00916, Metronidazole |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000321864 | 1 – 210 | Oxygen-insensitive NADPH nitroreductaseAdd BLAST | 210 |
Proteomic databases
PaxDbi | O25608 |
Interactioni
Protein-protein interaction databases
DIPi | DIP-3602N |
IntActi | O25608, 2 interactors |
MINTi | O25608 |
STRINGi | 85962.C694_04915 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O25608 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the nitroreductase family.Curated
Phylogenomic databases
eggNOGi | COG0778, Bacteria |
OMAi | MQNLYPE |
Family and domain databases
CDDi | cd02149, NfsB-like, 1 hit |
Gene3Di | 3.40.109.10, 1 hit |
InterProi | View protein in InterPro IPR033878, NfsB-like IPR029479, Nitroreductase IPR000415, Nitroreductase-like |
Pfami | View protein in Pfam PF00881, Nitroreductase, 1 hit |
SUPFAMi | SSF55469, SSF55469, 1 hit |
i Sequence
Sequence statusi: Complete.
O25608-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFLDQEKRR QLLNERHSCK MFDSHYEFSS TELEEIAEIA RLSPSSYNTQ
60 70 80 90 100
PWHFVMVTDK DLKKQIAAHS YFNEEMIKSA SALMVVCSLR PSELLPHGHY
110 120 130 140 150
MQNLYPESYK VRVIPSFAQM LGVRFNHSMQ RLESYILEQC YIAVGQICMG
160 170 180 190 200
VSLMGLDSCI IGGFDPLKVG EVLEERINKP KIACLIALGK RVAEASQKSR
210
KSKVDAITWL
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 14 | N → K in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. | 1 | |
Natural varianti | 31 | T → E in strain: HP500, 69A and ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. | 1 | |
Natural varianti | 36 | I → V in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. | 1 | |
Natural varianti | 53 | H → R in strain: ATCC 49503. | 1 | |
Natural varianti | 56 | M → I in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. | 1 | |
Natural varianti | 59 | D → N in strain: HP500, 69A, ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole and ATCC 49503 / 60190. | 1 | |
Natural varianti | 63 – 93 | KKQIA…LRPSE → NHPSRNPKH in strain: ATCC 43504 / NCTC 11637 / JCM 7653 / RPH 13487; resistant to metronidazole. Add BLAST | 31 | |
Natural varianti | 64 | K → N in strain: 69A. | 1 | |
Natural varianti | 68 | A → V in strain: HP500. | 1 | |
Natural varianti | 90 | R → K in strain: ATCC 49503 / 60190. | 1 | |
Natural varianti | 97 | H → T in strain: 69A. | 1 | |
Natural varianti | 98 | G → S in strain: ATCC 49503 / 60190. | 1 | |
Natural varianti | 106 | P → S in strain: 69A. | 1 | |
Natural varianti | 131 | R → K in strain: 69A. | 1 | |
Natural varianti | 172 | V → I in strain: 69A. | 1 | |
Natural varianti | 183 | A → V in strain: ATCC 49503 / 60190. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF012552 Genomic DNA Translation: AAC46349.1 AF315501 Genomic DNA Translation: AAK17124.1 AF315502 Genomic DNA Translation: AAK17125.1 AF316109 Genomic DNA Translation: AAK19260.1 AE000511 Genomic DNA Translation: AAD07997.1 |
PIRi | B64639 |
RefSeqi | NP_207746.1, NC_000915.1 WP_000670110.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07997; AAD07997; HP_0954 |
KEGGi | hpy:HP0954 |
PATRICi | fig|85962.47.peg.1022 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF012552 Genomic DNA Translation: AAC46349.1 AF315501 Genomic DNA Translation: AAK17124.1 AF315502 Genomic DNA Translation: AAK17125.1 AF316109 Genomic DNA Translation: AAK19260.1 AE000511 Genomic DNA Translation: AAD07997.1 |
PIRi | B64639 |
RefSeqi | NP_207746.1, NC_000915.1 WP_000670110.1, NC_018939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3QDL | X-ray | 2.00 | A/B/C/D | 1-210 | [»] | |
SMRi | O25608 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-3602N |
IntActi | O25608, 2 interactors |
MINTi | O25608 |
STRINGi | 85962.C694_04915 |
Chemistry databases
DrugBanki | DB00916, Metronidazole |
Proteomic databases
PaxDbi | O25608 |
Genome annotation databases
EnsemblBacteriai | AAD07997; AAD07997; HP_0954 |
KEGGi | hpy:HP0954 |
PATRICi | fig|85962.47.peg.1022 |
Phylogenomic databases
eggNOGi | COG0778, Bacteria |
OMAi | MQNLYPE |
Enzyme and pathway databases
BioCyci | HPY:HP0954-MONOMER |
Family and domain databases
CDDi | cd02149, NfsB-like, 1 hit |
Gene3Di | 3.40.109.10, 1 hit |
InterProi | View protein in InterPro IPR033878, NfsB-like IPR029479, Nitroreductase IPR000415, Nitroreductase-like |
Pfami | View protein in Pfam PF00881, Nitroreductase, 1 hit |
SUPFAMi | SSF55469, SSF55469, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RDXA_HELPY | |
Accessioni | O25608Primary (citable) accession number: O25608 Secondary accession number(s): O30585 Q9AHF8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 94 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families