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UniProtKB - O25516 (THIM_HELPY)
Protein
Hydroxyethylthiazole kinase
Gene
thiM
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).
UniRule annotationCatalytic activityi
- 5-(2-hydroxyethyl)-4-methylthiazole + ATP = 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H+UniRule annotationEC:2.7.1.50UniRule annotation
Cofactori
Mg2+UniRule annotation
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 37 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 113 | ATPUniRule annotation | 1 | |
Binding sitei | 158 | ATPUniRule annotation | 1 | |
Binding sitei | 185 | Substrate; via amide nitrogenUniRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- hydroxyethylthiazole kinase activity Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- thiamine biosynthetic process Source: UniProtKB-KW
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Thiamine biosynthesis |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00060;UER00139 |
Names & Taxonomyi
Protein namesi | Recommended name: Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)Alternative name(s): 4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation Short name: TH kinaseUniRule annotation Short name: Thz kinaseUniRule annotation |
Gene namesi | Name:thiMUniRule annotation Ordered Locus Names:HP_0845 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000156937 | 1 – 259 | Hydroxyethylthiazole kinaseAdd BLAST | 259 |
Proteomic databases
PaxDbi | O25516 |
Interactioni
Protein-protein interaction databases
IntActi | O25516, 1 interactor |
STRINGi | 85962.C694_04330 |
Family & Domainsi
Sequence similaritiesi
Belongs to the Thz kinase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG2145, Bacteria |
OMAi | KRPLVHN |
PhylomeDBi | O25516 |
Family and domain databases
CDDi | cd01170, THZ_kinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_00228, Thz_kinase, 1 hit |
InterProi | View protein in InterPro IPR000417, Hyethyz_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF02110, HK, 1 hit |
PIRSFi | PIRSF000513, Thz_kinase, 1 hit |
PRINTSi | PR01099, HYETHTZKNASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00694, thiM, 1 hit |
i Sequence
Sequence statusi: Complete.
O25516-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLKELRQKRP LVHNITNYVA AQFVANGLLA LGASPLMSDA IDEMRDLAKI
60 70 80 90 100
SDALAINIGT LNDRAILCAK EAIKHYKALN KPIVLDPVGC SASALRHDTS
110 120 130 140 150
LELLKSGGIS ALRGNAAELG SLVGISCESK GLDSNDAATP VEIIKLAAQK
160 170 180 190 200
YSVIAVMTGK TDYVSDGKKV LSITGGSEYL ALITGAGCLH AAACASFLSL
210 220 230 240 250
KKDPLDSMAQ LCALYKQAAF NAQKKVLENN GSNGSFLFYF LDALSLPIEL
ENSLIKEEW
Sequence cautioni
The sequence AAD07891 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07891.1 Different initiation. |
PIRi | E64625 |
RefSeqi | NP_207638.1, NC_000915.1 WP_015056066.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07891; AAD07891; HP_0845 |
KEGGi | hpy:HP_0845 |
PATRICi | fig|85962.47.peg.899 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07891.1 Different initiation. |
PIRi | E64625 |
RefSeqi | NP_207638.1, NC_000915.1 WP_015056066.1, NC_018939.1 |
3D structure databases
SMRi | O25516 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O25516, 1 interactor |
STRINGi | 85962.C694_04330 |
Proteomic databases
PaxDbi | O25516 |
Genome annotation databases
EnsemblBacteriai | AAD07891; AAD07891; HP_0845 |
KEGGi | hpy:HP_0845 |
PATRICi | fig|85962.47.peg.899 |
Phylogenomic databases
eggNOGi | COG2145, Bacteria |
OMAi | KRPLVHN |
PhylomeDBi | O25516 |
Enzyme and pathway databases
UniPathwayi | UPA00060;UER00139 |
Family and domain databases
CDDi | cd01170, THZ_kinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_00228, Thz_kinase, 1 hit |
InterProi | View protein in InterPro IPR000417, Hyethyz_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF02110, HK, 1 hit |
PIRSFi | PIRSF000513, Thz_kinase, 1 hit |
PRINTSi | PR01099, HYETHTZKNASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR00694, thiM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | THIM_HELPY | |
Accessioni | O25516Primary (citable) accession number: O25516 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | December 1, 2000 | |
Last modified: | February 23, 2022 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families