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Protein

Beta sliding clamp

Gene

dnaN

Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.By similarity

GO - Molecular functioni

  • 3'-5' exonuclease activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • DNA-directed DNA polymerase activity Source: UniProtKB-KW
  • identical protein binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase
Biological processDNA replication

Enzyme and pathway databases

BioCyciHPY:HP0500-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Beta sliding clamp
Short name:
Beta clamp
Short name:
Sliding clamp
Alternative name(s):
Beta-clamp processivity factor
DNA polymerase III beta sliding clamp subunit
DNA polymerase III subunit beta
Gene namesi
Name:dnaN
Ordered Locus Names:HP_0500
OrganismiHelicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Taxonomic identifieri85962 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000000429 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001054381 – 374Beta sliding clampAdd BLAST374

Proteomic databases

PaxDbiO25242

Interactioni

Subunit structurei

Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
HP_0418O251723EBI-7532786,EBI-9262675

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-3288N
IntActiO25242, 2 interactors
MINTiO25242
STRINGi85962.HP0500

Structurei

Secondary structure

1374
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO25242
SMRiO25242
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta sliding clamp family.Curated

Phylogenomic databases

eggNOGiENOG4105CZ8 Bacteria
COG0592 LUCA
KOiK02338
OMAiNYEAVIP

Family and domain databases

CDDicd00140 beta_clamp, 1 hit
InterProiView protein in InterPro
IPR001001 DNA_polIII_beta
IPR022635 DNA_polIII_beta_C
IPR022637 DNA_polIII_beta_cen
IPR022634 DNA_polIII_beta_N
PANTHERiPTHR30478 PTHR30478, 1 hit
PfamiView protein in Pfam
PF00712 DNA_pol3_beta, 1 hit
PF02767 DNA_pol3_beta_2, 1 hit
PF02768 DNA_pol3_beta_3, 1 hit
SMARTiView protein in SMART
SM00480 POL3Bc, 1 hit
TIGRFAMsiTIGR00663 dnan, 1 hit

Sequencei

Sequence statusi: Complete.

O25242-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKISVSKNDL ENALRYLQAF LDKKDASSIA SHIHLEVIKE KLFLKASDSD
60 70 80 90 100
IGLKSYIFTQ SSDKEGVGTI NGKKFLDIIS CLKDSNIILE TKDDSLAIKQ
110 120 130 140 150
NKSSFKLPMF DADEFPEFPV IDPKVSIEVN APFLVDAFKK IAPVIEQTSH
160 170 180 190 200
KRELAGILMQ FDQKHQTLSV VGTDTKRLSY TQLEKISIHS TEEDISCILP
210 220 230 240 250
KRALLEILKL FYENFSFKSD GMLAVIENEM HTFFTKLIDG NYPDYQKILP
260 270 280 290 300
KEYISSFTLG KEEFKESIKL CSSLSSTIKL TLEKNNALFE SLDSEHSETA
310 320 330 340 350
KTSVEIEKGL DIEKAFHLGV NAKFFLEALN ALGTTQFVLR CNEPSSPFLI
360 370
QESLDEKQSH LNAKISTLMM PITL
Length:374
Mass (Da):42,185
Last modified:January 1, 1998 - v1
Checksum:i5C1FE1C6290FDE6C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD07565.1
PIRiD64582
RefSeqiNP_207297.1, NC_000915.1
WP_000704186.1, NC_018939.1

Genome annotation databases

EnsemblBacteriaiAAD07565; AAD07565; HP_0500
GeneIDi899259
KEGGiheo:C694_02570
hpy:HP0500
PATRICifig|85962.47.peg.538

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000511 Genomic DNA Translation: AAD07565.1
PIRiD64582
RefSeqiNP_207297.1, NC_000915.1
WP_000704186.1, NC_018939.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4RKIX-ray2.05A1-374[»]
4S3IX-ray1.95A/B1-374[»]
5FRQX-ray2.90A/B/C/D1-374[»]
5FVEX-ray2.07A1-374[»]
5FXTX-ray1.97A1-374[»]
5G48X-ray2.28A/B1-374[»]
5G4QX-ray2.30A/B1-374[»]
ProteinModelPortaliO25242
SMRiO25242
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-3288N
IntActiO25242, 2 interactors
MINTiO25242
STRINGi85962.HP0500

Proteomic databases

PaxDbiO25242

Protocols and materials databases

DNASUi899259
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAD07565; AAD07565; HP_0500
GeneIDi899259
KEGGiheo:C694_02570
hpy:HP0500
PATRICifig|85962.47.peg.538

Phylogenomic databases

eggNOGiENOG4105CZ8 Bacteria
COG0592 LUCA
KOiK02338
OMAiNYEAVIP

Enzyme and pathway databases

BioCyciHPY:HP0500-MONOMER

Family and domain databases

CDDicd00140 beta_clamp, 1 hit
InterProiView protein in InterPro
IPR001001 DNA_polIII_beta
IPR022635 DNA_polIII_beta_C
IPR022637 DNA_polIII_beta_cen
IPR022634 DNA_polIII_beta_N
PANTHERiPTHR30478 PTHR30478, 1 hit
PfamiView protein in Pfam
PF00712 DNA_pol3_beta, 1 hit
PF02767 DNA_pol3_beta_2, 1 hit
PF02768 DNA_pol3_beta_3, 1 hit
SMARTiView protein in SMART
SM00480 POL3Bc, 1 hit
TIGRFAMsiTIGR00663 dnan, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPO3B_HELPY
AccessioniPrimary (citable) accession number: O25242
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Helicobacter pylori
    Helicobacter pylori (strain 26695): entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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