UniProtKB - O25242 (DPO3B_HELPY)
Beta sliding clamp
dnaN
Functioni
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.
By similarityGO - Molecular functioni
- 3'-5' exonuclease activity Source: InterPro
- DNA binding Source: UniProtKB-KW
- DNA-directed DNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- DNA strand elongation involved in DNA replication Source: GO_Central
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Nucleotidyltransferase, Transferase |
Biological process | DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: Beta sliding clampShort name: Beta clamp Short name: Sliding clamp Alternative name(s): Beta-clamp processivity factor DNA polymerase III beta sliding clamp subunit DNA polymerase III subunit beta |
Gene namesi | Name:dnaN Ordered Locus Names:HP_0500 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
- DNA polymerase III complex Source: InterPro
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105438 | 1 – 374 | Beta sliding clampAdd BLAST | 374 |
Proteomic databases
PaxDbi | O25242 |
Interactioni
Subunit structurei
Forms a ring-shaped head-to-tail homodimer around DNA which binds and tethers DNA polymerases and other proteins to the DNA. The DNA replisome complex has a single clamp-loading complex (3 tau and 1 each of delta, delta', psi and chi subunits) which binds 3 Pol III cores (1 core on the leading strand and 2 on the lagging strand) each with a beta sliding clamp dimer. Additional proteins in the replisome are other copies of gamma, psi and chi, Ssb, DNA helicase and RNA primase.
By similarityBinary interactionsi
O25242
With | #Exp. | IntAct |
---|---|---|
HP_0418 [O25172] | 3 | EBI-7532786,EBI-9262675 |
Protein-protein interaction databases
DIPi | DIP-3288N |
IntActi | O25242, 2 interactors |
MINTi | O25242 |
STRINGi | 85962.C694_02570 |
Structurei
Secondary structure
3D structure databases
SMRi | O25242 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0592, Bacteria |
OMAi | YLIMPVR |
PhylomeDBi | O25242 |
Family and domain databases
CDDi | cd00140, beta_clamp, 1 hit |
InterProi | View protein in InterPro IPR001001, DNA_polIII_beta IPR022635, DNA_polIII_beta_C IPR022637, DNA_polIII_beta_cen IPR022634, DNA_polIII_beta_N |
PANTHERi | PTHR30478, PTHR30478, 1 hit |
Pfami | View protein in Pfam PF00712, DNA_pol3_beta, 1 hit PF02767, DNA_pol3_beta_2, 1 hit PF02768, DNA_pol3_beta_3, 1 hit |
SMARTi | View protein in SMART SM00480, POL3Bc, 1 hit |
TIGRFAMsi | TIGR00663, dnan, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKISVSKNDL ENALRYLQAF LDKKDASSIA SHIHLEVIKE KLFLKASDSD
60 70 80 90 100
IGLKSYIFTQ SSDKEGVGTI NGKKFLDIIS CLKDSNIILE TKDDSLAIKQ
110 120 130 140 150
NKSSFKLPMF DADEFPEFPV IDPKVSIEVN APFLVDAFKK IAPVIEQTSH
160 170 180 190 200
KRELAGILMQ FDQKHQTLSV VGTDTKRLSY TQLEKISIHS TEEDISCILP
210 220 230 240 250
KRALLEILKL FYENFSFKSD GMLAVIENEM HTFFTKLIDG NYPDYQKILP
260 270 280 290 300
KEYISSFTLG KEEFKESIKL CSSLSSTIKL TLEKNNALFE SLDSEHSETA
310 320 330 340 350
KTSVEIEKGL DIEKAFHLGV NAKFFLEALN ALGTTQFVLR CNEPSSPFLI
360 370
QESLDEKQSH LNAKISTLMM PITL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07565.1 |
PIRi | D64582 |
RefSeqi | NP_207297.1, NC_000915.1 WP_000704186.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07565; AAD07565; HP_0500 |
KEGGi | hpy:HP_0500 |
PATRICi | fig|85962.47.peg.538 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07565.1 |
PIRi | D64582 |
RefSeqi | NP_207297.1, NC_000915.1 WP_000704186.1, NC_018939.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4RKI | X-ray | 2.05 | A | 1-374 | [»] | |
4S3I | X-ray | 1.95 | A/B | 1-374 | [»] | |
5FRQ | X-ray | 2.90 | A/B/C/D | 1-374 | [»] | |
5FVE | X-ray | 2.07 | A | 1-374 | [»] | |
5FXT | X-ray | 1.97 | A | 1-374 | [»] | |
5G48 | X-ray | 2.28 | A/B | 1-374 | [»] | |
5G4Q | X-ray | 2.30 | A/B | 1-374 | [»] | |
SMRi | O25242 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-3288N |
IntActi | O25242, 2 interactors |
MINTi | O25242 |
STRINGi | 85962.C694_02570 |
Proteomic databases
PaxDbi | O25242 |
Protocols and materials databases
DNASUi | 899259 |
Genome annotation databases
EnsemblBacteriai | AAD07565; AAD07565; HP_0500 |
KEGGi | hpy:HP_0500 |
PATRICi | fig|85962.47.peg.538 |
Phylogenomic databases
eggNOGi | COG0592, Bacteria |
OMAi | YLIMPVR |
PhylomeDBi | O25242 |
Family and domain databases
CDDi | cd00140, beta_clamp, 1 hit |
InterProi | View protein in InterPro IPR001001, DNA_polIII_beta IPR022635, DNA_polIII_beta_C IPR022637, DNA_polIII_beta_cen IPR022634, DNA_polIII_beta_N |
PANTHERi | PTHR30478, PTHR30478, 1 hit |
Pfami | View protein in Pfam PF00712, DNA_pol3_beta, 1 hit PF02767, DNA_pol3_beta_2, 1 hit PF02768, DNA_pol3_beta_3, 1 hit |
SMARTi | View protein in SMART SM00480, POL3Bc, 1 hit |
TIGRFAMsi | TIGR00663, dnan, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO3B_HELPY | |
Accessioni | O25242Primary (citable) accession number: O25242 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families