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UniProtKB - O25235 (MRAY_HELPY)
Protein
Phospho-N-acetylmuramoyl-pentapeptide-transferase
Gene
mraY
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Catalyzes the initial step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan: transfers peptidoglycan precursor phospho-MurNAc-pentapeptide from UDP-MurNAc-pentapeptide onto the lipid carrier undecaprenyl phosphate, yielding undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide, known as lipid I.
UniRule annotationCatalytic activityi
- di-trans,octa-cis-undecaprenyl phosphate + UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine = di-trans-octa-cis-undecaprenyl diphospho-N-acetyl-α-D-muramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + UMPUniRule annotationEC:2.7.8.13UniRule annotation
Cofactori
Mg2+UniRule annotation
: peptidoglycan biosynthesis Pathwayi
This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotationView all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: UniProtKB-UniRule
- phosphotransferase activity, for other substituted phosphate groups Source: GO_Central
- UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity Source: UniProtKB-EC
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- cell wall macromolecule biosynthetic process Source: GO_Central
- cell wall organization Source: GO_Central
- peptidoglycan biosynthetic process Source: UniProtKB-UniRule
- regulation of cell shape Source: UniProtKB-KW
Keywordsi
Molecular function | Transferase |
Biological process | Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Names & Taxonomyi
Protein namesi | Recommended name: Phospho-N-acetylmuramoyl-pentapeptide-transferaseUniRule annotation (EC:2.7.8.13UniRule annotation)Alternative name(s): UDP-MurNAc-pentapeptide phosphotransferaseUniRule annotation |
Gene namesi | Name:mraYUniRule annotation Ordered Locus Names:HP_0493 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 24 – 44 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 66 – 86 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 88 – 108 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 129 – 149 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 160 – 180 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 192 – 212 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 229 – 249 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 256 – 276 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 281 – 301 | HelicalUniRule annotationAdd BLAST | 21 | |
Transmembranei | 330 – 350 | HelicalUniRule annotationAdd BLAST | 21 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000108836 | 1 – 353 | Phospho-N-acetylmuramoyl-pentapeptide-transferaseAdd BLAST | 353 |
Proteomic databases
PaxDbi | O25235 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0472, Bacteria |
OMAi | LMSPLHH |
PhylomeDBi | O25235 |
Family and domain databases
CDDi | cd06852, GT_MraY, 1 hit |
HAMAPi | MF_00038, MraY, 1 hit |
InterProi | View protein in InterPro IPR000715, Glycosyl_transferase_4 IPR003524, PNAcMuramoyl-5peptid_Trfase IPR018480, PNAcMuramoyl-5peptid_Trfase_CS |
PANTHERi | PTHR22926, PTHR22926, 1 hit |
Pfami | View protein in Pfam PF00953, Glycos_transf_4, 1 hit PF10555, MraY_sig1, 1 hit |
TIGRFAMsi | TIGR00445, mraY, 1 hit |
PROSITEi | View protein in PROSITE PS01347, MRAY_1, 1 hit PS01348, MRAY_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O25235-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLYSLLYGYF NINLFQYLTF RAGLGFFIAF FLTLFLMPKF ILWAKAKKAN
60 70 80 90 100
QPISSFVPSH QNKKDTPTMG GIVFVFATIV ASVLCASLGN LYVLLGLIVL
110 120 130 140 150
VGFSFVGFRD DYTKINQQSN AGMSAKMKFG MLFILSLIVS VLLSLKGLDT
160 170 180 190 200
FLYAPFLKNP LFEMPTMLAV GFWVLVFLST SNAVNLTDGL DGLASVPSIF
210 220 230 240 250
TLLSLSIFVY VAGNAEFSKY LLYPKVIDVG ELFVVSLALV GSLFGFLWYN
260 270 280 290 300
CNPASVFMGD SGSLALGGFI AYNAIVSHNE ILLVLMGSIF VIETLSVILQ
310 320 330 340 350
VGSYKTRKKR LFLMAPIHHH FEQKGWAENK VIVRFWIISM LSNLVALLSL
KVR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07559.1 |
PIRi | E64581 |
RefSeqi | NP_207290.1, NC_000915.1 WP_000967129.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07559; AAD07559; HP_0493 |
KEGGi | hpy:HP_0493 |
PATRICi | fig|85962.47.peg.531 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07559.1 |
PIRi | E64581 |
RefSeqi | NP_207290.1, NC_000915.1 WP_000967129.1, NC_018939.1 |
3D structure databases
SMRi | O25235 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 85962.C694_02535 |
Proteomic databases
PaxDbi | O25235 |
Genome annotation databases
EnsemblBacteriai | AAD07559; AAD07559; HP_0493 |
KEGGi | hpy:HP_0493 |
PATRICi | fig|85962.47.peg.531 |
Phylogenomic databases
eggNOGi | COG0472, Bacteria |
OMAi | LMSPLHH |
PhylomeDBi | O25235 |
Enzyme and pathway databases
UniPathwayi | UPA00219 |
Family and domain databases
CDDi | cd06852, GT_MraY, 1 hit |
HAMAPi | MF_00038, MraY, 1 hit |
InterProi | View protein in InterPro IPR000715, Glycosyl_transferase_4 IPR003524, PNAcMuramoyl-5peptid_Trfase IPR018480, PNAcMuramoyl-5peptid_Trfase_CS |
PANTHERi | PTHR22926, PTHR22926, 1 hit |
Pfami | View protein in Pfam PF00953, Glycos_transf_4, 1 hit PF10555, MraY_sig1, 1 hit |
TIGRFAMsi | TIGR00445, mraY, 1 hit |
PROSITEi | View protein in PROSITE PS01347, MRAY_1, 1 hit PS01348, MRAY_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MRAY_HELPY | |
Accessioni | O25235Primary (citable) accession number: O25235 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 129 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families