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UniProtKB - O24854 (RISB_HELPY)
Protein
6,7-dimethyl-8-ribityllumazine synthase
Gene
ribH
Organism
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Status
Functioni
Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.
UniRule annotationCatalytic activityi
- (2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + H+ + 2 H2O + phosphateUniRule annotationEC:2.5.1.78UniRule annotation
: riboflavin biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil.UniRule annotation This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 23 | 5-amino-6-(D-ribitylamino)uracilUniRule annotation | 1 | |
Active sitei | 89 | Proton donorUniRule annotation | 1 | |
Binding sitei | 114 | 5-amino-6-(D-ribitylamino)uracil; via amide nitrogen and carbonyl oxygenUniRule annotation | 1 | |
Binding sitei | 128 | 1-deoxy-L-glycero-tetrulose 4-phosphateUniRule annotation | 1 |
GO - Molecular functioni
- 6,7-dimethyl-8-ribityllumazine synthase activity Source: GO_Central
GO - Biological processi
- riboflavin biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Riboflavin biosynthesis |
Enzyme and pathway databases
BRENDAi | 2.5.1.78, 2604 |
UniPathwayi | UPA00275;UER00404 |
Names & Taxonomyi
Protein namesi | Recommended name: 6,7-dimethyl-8-ribityllumazine synthaseUniRule annotation (EC:2.5.1.78UniRule annotation)Short name: DMRL synthaseUniRule annotation Short name: LSUniRule annotation Short name: Lumazine synthaseUniRule annotation |
Gene namesi | Name:ribHUniRule annotation Synonyms:ribE Ordered Locus Names:HP_0002 |
Organismi | Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) |
Taxonomic identifieri | 85962 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Helicobacteraceae › Helicobacter › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Other locations
- riboflavin synthase complex Source: InterPro
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000134763 | 1 – 156 | 6,7-dimethyl-8-ribityllumazine synthaseAdd BLAST | 156 |
Proteomic databases
PaxDbi | O24854 |
Interactioni
Protein-protein interaction databases
DIPi | DIP-3051N |
IntActi | O24854, 4 interactors |
MINTi | O24854 |
STRINGi | 85962.C694_00010 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 57 – 59 | 5-amino-6-(D-ribitylamino)uracil bindingUniRule annotation | 3 | |
Regioni | 81 – 83 | 5-amino-6-(D-ribitylamino)uracil bindingUniRule annotation | 3 | |
Regioni | 86 – 87 | 1-deoxy-L-glycero-tetrulose 4-phosphate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the DMRL synthase family.UniRule annotation
Phylogenomic databases
eggNOGi | COG0054, Bacteria |
OMAi | CQGVTQG |
PhylomeDBi | O24854 |
Family and domain databases
CDDi | cd09209, Lumazine_synthase-I, 1 hit |
Gene3Di | 3.40.50.960, 1 hit |
HAMAPi | MF_00178, Lumazine_synth, 1 hit |
InterProi | View protein in InterPro IPR034964, LS IPR002180, LS/RS IPR036467, LS/RS_sf |
PANTHERi | PTHR21058, PTHR21058, 1 hit |
Pfami | View protein in Pfam PF00885, DMRL_synthase, 1 hit |
SUPFAMi | SSF52121, SSF52121, 1 hit |
TIGRFAMsi | TIGR00114, lumazine-synth, 1 hit |
i Sequence
Sequence statusi: Complete.
O24854-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQIIEGKLQL QGNERVAILT SRFNHIITDR LQEGAMDCFK RHGGDEDLLD
60 70 80 90 100
IVLVPGAYEL PFILDKLLES EKYDGVCVLG AIIRGGTPHF DYVSAEATKG
110 120 130 140 150
IAHAMLKYSM PVSFGVLTTD NIEQAIERAG SKAGNKGFEA MSTLIELLSL
CQTLKG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07075.1 |
PIRi | B64520 |
RefSeqi | NP_206804.1, NC_000915.1 WP_001165637.1, NC_018939.1 |
Genome annotation databases
EnsemblBacteriai | AAD07075; AAD07075; HP_0002 |
KEGGi | hpy:HP_0002 |
PATRICi | fig|85962.47.peg.2 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000511 Genomic DNA Translation: AAD07075.1 |
PIRi | B64520 |
RefSeqi | NP_206804.1, NC_000915.1 WP_001165637.1, NC_018939.1 |
3D structure databases
AlphaFoldDBi | O24854 |
SMRi | O24854 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-3051N |
IntActi | O24854, 4 interactors |
MINTi | O24854 |
STRINGi | 85962.C694_00010 |
Proteomic databases
PaxDbi | O24854 |
Genome annotation databases
EnsemblBacteriai | AAD07075; AAD07075; HP_0002 |
KEGGi | hpy:HP_0002 |
PATRICi | fig|85962.47.peg.2 |
Phylogenomic databases
eggNOGi | COG0054, Bacteria |
OMAi | CQGVTQG |
PhylomeDBi | O24854 |
Enzyme and pathway databases
UniPathwayi | UPA00275;UER00404 |
BRENDAi | 2.5.1.78, 2604 |
Family and domain databases
CDDi | cd09209, Lumazine_synthase-I, 1 hit |
Gene3Di | 3.40.50.960, 1 hit |
HAMAPi | MF_00178, Lumazine_synth, 1 hit |
InterProi | View protein in InterPro IPR034964, LS IPR002180, LS/RS IPR036467, LS/RS_sf |
PANTHERi | PTHR21058, PTHR21058, 1 hit |
Pfami | View protein in Pfam PF00885, DMRL_synthase, 1 hit |
SUPFAMi | SSF52121, SSF52121, 1 hit |
TIGRFAMsi | TIGR00114, lumazine-synth, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RISB_HELPY | |
Accessioni | O24854Primary (citable) accession number: O24854 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 118 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families