UniProtKB - O24592 (NCED1_MAIZE)
Protein
9-cis-epoxycarotenoid dioxygenase 1, chloroplastic
Gene
VP14
Organism
Zea mays (Maize)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids. Not active on the all-trans isomers of violaxanthin and neoxanthin. Contributes probably to abscisic acid synthesis for the induction of seed dormancy.3 Publications
Catalytic activityi
- EC:1.13.11.512 Publications
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxin2 PublicationsEC:1.13.11.512 Publications
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxin2 PublicationsEC:1.13.11.512 Publications
Cofactori
Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication
Kineticsi
Vmax with 9-cis violaxanthin as substrate is more than twice the Vmax with 9-cis neoxanthin as substrate.
- KM=20 µM for 9-cis antheraxanthin1 Publication
- KM=15 µM for 9-cis luteoxanthin 8'S1 Publication
- KM=8 µM for 9-cis luteoxanthin 8'R1 Publication
- KM=27 µM for 9-cis neoxanthin1 Publication
- KM=58 µM for 9-cis violaxanthin1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 298 | Iron; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 347 | Iron; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 412 | Iron; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 590 | Iron; via tele nitrogenCombined sources1 Publication | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: UniProtKB-EC
- carotenoid dioxygenase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: UniProtKB-KW
- carotene catabolic process Source: GO_Central
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis, Stress response |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.13.11.51, 6752 |
Chemistry databases
SwissLipidsi | SLP:000001487 |
Names & Taxonomyi
Protein namesi | Recommended name: 9-cis-epoxycarotenoid dioxygenase 1, chloroplastic (EC:1.13.11.512 Publications)Alternative name(s): Protein VIVIPAROUS141 Publication Short name: VP-14 Short name: VP141 Publication |
Gene namesi | Name:VP14 |
Organismi | Zea mays (Maize) |
Taxonomic identifieri | 4577 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Poaceae › PACMAD clade › Panicoideae › Andropogonodae › Andropogoneae › Tripsacinae › Zea |
Proteomesi |
|
Organism-specific databases
MaizeGDBi | 726071 |
Subcellular locationi
Chloroplast
- chloroplast stroma 2 Publications
Note: Partially bound to the thylakoid membranes.
Chloroplast
- chloroplast Source: GO_Central
- chloroplast stroma Source: GO_Central
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Plants show a viviparous phenotype.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 | A → AGGG: Loss of binding to the thylakoid membrane. 1 Publication | 1 | |
Mutagenesisi | 90 | A → APPP: Loss of binding to the thylakoid membrane. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 23 | ChloroplastSequence analysisAdd BLAST | 23 | |
ChainiPRO_0000282607 | 24 – 604 | 9-cis-epoxycarotenoid dioxygenase 1, chloroplasticAdd BLAST | 581 |
Proteomic databases
PaxDbi | O24592 |
Expressioni
Tissue specificityi
Expressed in leaves, roots and embryos.1 Publication
Inductioni
By drought stress.1 Publication
Gene expression databases
ExpressionAtlasi | O24592, baseline and differential |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O24592 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O24592 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 90 – 94 | Poly-Ala | 5 |
Domaini
The N-terminal amphipathic alpha-helix (81-100) is essential, but not sufficient, for the thylakoid membrane targeting.
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
OrthoDBi | 524712at2759 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O24592-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQGLAPPTSV SIHRHLPARS RARASNSVRF SPRAVSSVPP AECLQAPFHK
60 70 80 90 100
PVADLPAPSR KPAAIAVPGH AAAPRKAEGG KKQLNLFQRA AAAALDAFEE
110 120 130 140 150
GFVANVLERP HGLPSTADPA VQIAGNFAPV GERPPVHELP VSGRIPPFID
160 170 180 190 200
GVYARNGANP CFDPVAGHHL FDGDGMVHAL RIRNGAAESY ACRFTETARL
210 220 230 240 250
RQERAIGRPV FPKAIGELHG HSGIARLALF YARAACGLVD PSAGTGVANA
260 270 280 290 300
GLVYFNGRLL AMSEDDLPYH VRVADDGDLE TVGRYDFDGQ LGCAMIAHPK
310 320 330 340 350
LDPATGELHA LSYDVIKRPY LKYFYFRPDG TKSDDVEIPL EQPTMIHDFA
360 370 380 390 400
ITENLVVVPD HQVVFKLQEM LRGGSPVVLD KEKTSRFGVL PKHAADASEM
410 420 430 440 450
AWVDVPDCFC FHLWNAWEDE ATGEVVVIGS CMTPADSIFN ESDERLESVL
460 470 480 490 500
TEIRLDARTG RSTRRAVLPP SQQVNLEVGM VNRNLLGRET RYAYLAVAEP
510 520 530 540 550
WPKVSGFAKV DLSTGELTKF EYGEGRFGGE PCFVPMDPAA AHPRGEDDGY
560 570 580 590 600
VLTFVHDERA GTSELLVVNA ADMRLEATVQ LPSRVPFGFH GTFITGQELE
AQAA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95953 mRNA Translation: AAB62181.2 |
PIRi | T04351 |
RefSeqi | NP_001105902.2, NM_001112432.2 |
Genome annotation databases
GeneIDi | 732819 |
KEGGi | zma:732819 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U95953 mRNA Translation: AAB62181.2 |
PIRi | T04351 |
RefSeqi | NP_001105902.2, NM_001112432.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3NPE | X-ray | 3.20 | A | 76-604 | [»] | |
SMRi | O24592 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 4577.GRMZM2G014392_P01 |
Chemistry databases
SwissLipidsi | SLP:000001487 |
Proteomic databases
PaxDbi | O24592 |
Genome annotation databases
GeneIDi | 732819 |
KEGGi | zma:732819 |
Organism-specific databases
MaizeGDBi | 726071 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
OrthoDBi | 524712at2759 |
Enzyme and pathway databases
BRENDAi | 1.13.11.51, 6752 |
Miscellaneous databases
EvolutionaryTracei | O24592 |
Gene expression databases
ExpressionAtlasi | O24592, baseline and differential |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED1_MAIZE | |
Accessioni | O24592Primary (citable) accession number: O24592 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | September 22, 2009 | |
Last modified: | December 2, 2020 | |
This is version 92 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families