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UniProtKB - O24495 (GLO2M_ARATH)
Protein
Hydroxyacylglutathione hydrolase 1, mitochondrial
Gene
GLX2-1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.
Catalytic activityi
- EC:3.1.2.6
Cofactori
Fe2+By similarity, Fe3+By similarity, Zn2+By similarityNote: Binds 1 Fe2+ or Fe3+ and 1 Zn2+ ion per subunit. Electron spin resonance indicates the presence of a mixture of protein molecules that contain either Fe2+ or Zn2+.By similarity
: methylglyoxal degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal. This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 131 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 133 | Zinc; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 135 | IronBy similarity | 1 | |
Metal bindingi | 136 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 189 | Zinc; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 208 | IronBy similarity | 1 | |
Metal bindingi | 208 | ZincBy similarity | 1 |
GO - Molecular functioni
- beta-lactamase activity Source: TAIR
- hydroxyacylglutathione hydrolase activity Source: GO_Central
- metal ion binding Source: TAIR
- zinc ion binding Source: InterPro
GO - Biological processi
- antibiotic catabolic process Source: InterPro
- methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione Source: InterPro
- response to amino acid Source: TAIR
- response to anoxia Source: TAIR
- response to salt stress Source: TAIR
Keywordsi
Molecular function | Hydrolase |
Ligand | Iron, Metal-binding, Zinc |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00676 |
Names & Taxonomyi
Protein namesi | Recommended name: Hydroxyacylglutathione hydrolase 1, mitochondrial (EC:3.1.2.6)Alternative name(s): Glyoxalase II Short name: Glx II |
Gene namesi | Name:GLX2-1 Synonyms:GLY1 Ordered Locus Names:At2g43430 ORF Names:T1O24.17 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G43430 |
TAIRi | locus:2058239, AT2G43430 |
Subcellular locationi
Mitochondrion
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 76 | MitochondrionSequence analysisAdd BLAST | 76 | |
ChainiPRO_0000012286 | 77 – 331 | Hydroxyacylglutathione hydrolase 1, mitochondrialAdd BLAST | 255 |
Proteomic databases
PaxDbi | O24495 |
PRIDEi | O24495 |
ProteomicsDBi | 248585 [O24495-1] |
Expressioni
Tissue specificityi
Mainly expressed in roots, flowers and flower buds. Also detected in leaves.1 Publication
Gene expression databases
ExpressionAtlasi | O24495, baseline and differential |
Genevisiblei | O24495, AT |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 246 – 248 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0813, Eukaryota |
InParanoidi | O24495 |
OrthoDBi | 961826at2759 |
PhylomeDBi | O24495 |
Family and domain databases
CDDi | cd07723, hydroxyacylglutathione_hydrolase_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01374, Glyoxalase_2, 1 hit |
InterProi | View protein in InterPro IPR001018, Beta-lactamase_class-B_CS IPR035680, Clx_II_MBL IPR032282, HAGH_C IPR017782, Hydroxyacylglutathione_Hdrlase IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF16123, HAGH_C, 1 hit PF00753, Lactamase_B, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03413, GSH_gloB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O24495-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPVISKASST TTNSSIPSCS RIGGQLCVWP GLRQLCLRKS LLYGVMWLLS
60 70 80 90 100
MPLKTLRGAR KTLKITHFCS ISNMPSSLKI ELVPCSKDNY AYLLHDEDTG
110 120 130 140 150
TVGVVDPSEA APVIEALSRK NWNLTYILNT HHHDDHIGGN AELKERYGAK
160 170 180 190 200
VIGSAVDKDR IPGIDILLKD SDKWMFAGHE VRILDTPGHT QGHISFYFPG
210 220 230 240 250
SATIFTGDLI YSLSCGTLSE GTPEQMLSSL QKIVSLPDDT NIYCGRENTA
260 270 280 290 300
GNLKFALSVE PKNETLQSYA TRVAHLRSQG LPSIPTTVKV EKACNPFLRI
310 320 330
SSKDIRKSLS IPDSATEAEA LRRIQRARDR F
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4IR49 | F4IR49_ARATH | Glyoxalase 2-1 | GLX2-1 GLY1, glyoxalase 2-1, GLYOXALASE II, At2g43430, T1O24.17 | 313 | Annotation score: | ||
A0A178VWG4 | A0A178VWG4_ARATH | Glyoxalase 2-1 | GLX2-1 GLY1, glyoxalase 2-1, GLYOXALASE II, At2g43430, T1O24.17 | 286 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 | D → H in AAC49866 (PubMed:9349270).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U90927 Genomic DNA Translation: AAC49865.1 U90928 mRNA Translation: AAC49866.1 AC002335 Genomic DNA Translation: AAB64315.2 CP002685 Genomic DNA Translation: AEC10268.1 AY091278 mRNA Translation: AAM14217.1 AY063806 mRNA Translation: AAL36162.1 |
PIRi | A84866 |
RefSeqi | NP_565999.1, NM_129904.3 [O24495-1] |
Genome annotation databases
EnsemblPlantsi | AT2G43430.1; AT2G43430.1; AT2G43430 [O24495-1] |
GeneIDi | 818944 |
Gramenei | AT2G43430.1; AT2G43430.1; AT2G43430 [O24495-1] |
KEGGi | ath:AT2G43430 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U90927 Genomic DNA Translation: AAC49865.1 U90928 mRNA Translation: AAC49866.1 AC002335 Genomic DNA Translation: AAB64315.2 CP002685 Genomic DNA Translation: AEC10268.1 AY091278 mRNA Translation: AAM14217.1 AY063806 mRNA Translation: AAL36162.1 |
PIRi | A84866 |
RefSeqi | NP_565999.1, NM_129904.3 [O24495-1] |
3D structure databases
SMRi | O24495 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G43430.1 |
Proteomic databases
PaxDbi | O24495 |
PRIDEi | O24495 |
ProteomicsDBi | 248585 [O24495-1] |
Genome annotation databases
EnsemblPlantsi | AT2G43430.1; AT2G43430.1; AT2G43430 [O24495-1] |
GeneIDi | 818944 |
Gramenei | AT2G43430.1; AT2G43430.1; AT2G43430 [O24495-1] |
KEGGi | ath:AT2G43430 |
Organism-specific databases
Araporti | AT2G43430 |
TAIRi | locus:2058239, AT2G43430 |
Phylogenomic databases
eggNOGi | KOG0813, Eukaryota |
InParanoidi | O24495 |
OrthoDBi | 961826at2759 |
PhylomeDBi | O24495 |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00676 |
Miscellaneous databases
PROi | PR:O24495 |
Gene expression databases
ExpressionAtlasi | O24495, baseline and differential |
Genevisiblei | O24495, AT |
Family and domain databases
CDDi | cd07723, hydroxyacylglutathione_hydrolase_MBL-fold, 1 hit |
Gene3Di | 3.60.15.10, 1 hit |
HAMAPi | MF_01374, Glyoxalase_2, 1 hit |
InterProi | View protein in InterPro IPR001018, Beta-lactamase_class-B_CS IPR035680, Clx_II_MBL IPR032282, HAGH_C IPR017782, Hydroxyacylglutathione_Hdrlase IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF16123, HAGH_C, 1 hit PF00753, Lactamase_B, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
TIGRFAMsi | TIGR03413, GSH_gloB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GLO2M_ARATH | |
Accessioni | O24495Primary (citable) accession number: O24495 Secondary accession number(s): O22857, O24494 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | May 27, 2002 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families