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Protein

Pinene synthase, chloroplastic

Gene

ag3

Organism
Abies grandis (Grand fir) (Pinus grandis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in monoterpene (C10) olefins biosynthesis. A mixture of alpha- and beta-pinene is produced by this enzyme.1 Publication

Miscellaneous

The conserved 64-Arg-Arg-65 motif may play a role in the isomerization step of the terpenoid cyclization reaction sequence.

Catalytic activityi

Geranyl-diphosphate = (-)-alpha-pinene + diphosphate.1 Publication
Geranyl diphosphate = (-)-beta-pinene + diphosphate.1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity, Mn2+By similarityNote: Binds 3 Mg2+ or Mn2+ ions per subunit.By similarity
  • K+

Pathwayi: oleoresin biosynthesis

This protein is involved in the pathway oleoresin biosynthesis, which is part of Terpene metabolism.
View all proteins of this organism that are known to be involved in the pathway oleoresin biosynthesis and in Terpene metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi379Magnesium or manganese 1By similarity1
Metal bindingi379Magnesium or manganese 2By similarity1
Metal bindingi383Magnesium or manganese 1By similarity1
Metal bindingi383Magnesium or manganese 2By similarity1
Metal bindingi531Magnesium or manganese 3By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionLyase
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12819
BRENDAi4.2.3.119 2
4.2.3.120 2
UniPathwayi
UPA00924

Names & Taxonomyi

Protein namesi
Recommended name:
Pinene synthase, chloroplastic (EC:4.2.3.119, EC:4.2.3.120)
Alternative name(s):
(-)-(1S,5S)-pinene synthase
Agg-pin1
Beta-geraniolene synthase
Gene namesi
Name:ag3
Synonyms:AG3.18
OrganismiAbies grandis (Grand fir) (Pinus grandis)
Taxonomic identifieri46611 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaePinalesPinaceaeAbies

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 36ChloroplastSequence analysisAdd BLAST36
ChainiPRO_000003362837 – 628Pinene synthase, chloroplasticAdd BLAST592

Expressioni

Inductioni

By wounding.1 Publication

Structurei

3D structure databases

ProteinModelPortaliO24475
SMRiO24475
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi379 – 383DDXXD motif5

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family. Tpsd subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

KOiK07384

Family and domain databases

Gene3Di1.10.600.10, 1 hit
1.50.10.130, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR034741 Terpene_cyclase_like_1_C
IPR001906 Terpene_synth_N
IPR036965 Terpene_synth_N_sf
IPR005630 Terpene_synthase_metal-bd
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF01397 Terpene_synth, 1 hit
PF03936 Terpene_synth_C, 1 hit
SFLDiSFLDG01019 Terpene_Cyclase_Like_1_C_Termi, 1 hit
SUPFAMiSSF48239 SSF48239, 1 hit
SSF48576 SSF48576, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24475-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALVSTAPLA SKSCLHKSLI SSTHELKALS RTIPALGMSR RGKSITPSIS
60 70 80 90 100
MSSTTVVTDD GVRRRMGDFH SNLWDDDVIQ SLPTAYEEKS YLERAEKLIG
110 120 130 140 150
EVKNMFNSMS LEDGELMSPL NDLIQRLWIV DSLERLGIHR HFKDEIKSAL
160 170 180 190 200
DYVYSYWGEN GIGCGRESVV TDLNSTALGL RTLRLHGYPV SSDVFKAFKG
210 220 230 240 250
QNGQFSCSEN IQTDEEIRGV LNLFRASLIA FPGEKIMDEA EIFSTKYLKE
260 270 280 290 300
ALQKIPVSSL SREIGDVLEY GWHTYLPRLE ARNYIQVFGQ DTENTKSYVK
310 320 330 340 350
SKKLLELAKL EFNIFQSLQK RELESLVRWW KESGFPEMTF CRHRHVEYYT
360 370 380 390 400
LASCIAFEPQ HSGFRLGFAK TCHLITVLDD MYDTFGTVDE LELFTATMKR
410 420 430 440 450
WDPSSIDCLP EYMKGVYIAV YDTVNEMARE AEEAQGRDTL TYAREAWEAY
460 470 480 490 500
IDSYMQEARW IATGYLPSFD EYYENGKVSC GHRISALQPI LTMDIPFPDH
510 520 530 540 550
ILKEVDFPSK LNDLACAILR LRGDTRCYKA DRARGEEASS ISCYMKDNPG
560 570 580 590 600
VSEEDALDHI NAMISDVIKG LNWELLKPDI NVPISAKKHA FDIARAFHYG
610 620
YKYRDGYSVA NVETKSLVTR TLLESVPL
Length:628
Mass (Da):71,506
Last modified:January 1, 1998 - v1
Checksum:i23DBB788F3C8072C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103K → E in AAK83564 (PubMed:11404343).Curated1
Sequence conflicti134E → G in AAK83564 (PubMed:11404343).Curated1
Sequence conflicti169V → A in AAK83564 (PubMed:11404343).Curated1
Sequence conflicti180L → F in AAK83564 (PubMed:11404343).Curated1
Sequence conflicti286Q → H in AAK83564 (PubMed:11404343).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87909 mRNA Translation: AAB71085.1
AF326517 Genomic DNA Translation: AAK83564.1

Genome annotation databases

KEGGiag:AAB71085

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U87909 mRNA Translation: AAB71085.1
AF326517 Genomic DNA Translation: AAK83564.1

3D structure databases

ProteinModelPortaliO24475
SMRiO24475
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAB71085

Phylogenomic databases

KOiK07384

Enzyme and pathway databases

UniPathwayi
UPA00924

BioCyciMetaCyc:MONOMER-12819
BRENDAi4.2.3.119 2
4.2.3.120 2

Family and domain databases

Gene3Di1.10.600.10, 1 hit
1.50.10.130, 1 hit
InterProiView protein in InterPro
IPR008949 Isoprenoid_synthase_dom_sf
IPR034741 Terpene_cyclase_like_1_C
IPR001906 Terpene_synth_N
IPR036965 Terpene_synth_N_sf
IPR005630 Terpene_synthase_metal-bd
IPR008930 Terpenoid_cyclase/PrenylTrfase
PfamiView protein in Pfam
PF01397 Terpene_synth, 1 hit
PF03936 Terpene_synth_C, 1 hit
SFLDiSFLDG01019 Terpene_Cyclase_Like_1_C_Termi, 1 hit
SUPFAMiSSF48239 SSF48239, 1 hit
SSF48576 SSF48576, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPSD3_ABIGR
AccessioniPrimary (citable) accession number: O24475
Secondary accession number(s): Q94FW0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2001
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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