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Protein

Elongation factor Tu, chloroplastic

Gene

tufA

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 105GTPBy similarity8
Nucleotide bindingi160 – 164GTPBy similarity5
Nucleotide bindingi215 – 218GTPBy similarity4

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • translation elongation factor activity Source: UniProtKB

GO - Biological processi

  • translational elongation Source: UniProtKB

Keywordsi

Molecular functionElongation factor
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu, chloroplastic
Short name:
EF-Tu
Gene namesi
Name:tufA
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 79ChloroplastSequence analysisAdd BLAST79
ChainiPRO_000000745780 – 488Elongation factor Tu, chloroplasticAdd BLAST409

Proteomic databases

PRIDEiO24310

Expressioni

Tissue specificityi

Higher expression in leaves than in roots.1 Publication

Inductioni

Induced by light, low temperature and salicylic acid (SA). Repressed by high salt and abscisic acid (ABA).1 Publication

Structurei

3D structure databases

ProteinModelPortaliO24310
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini89 – 293tr-type GAdd BLAST205

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 105G1By similarity8
Regioni139 – 143G2By similarity5
Regioni160 – 163G3By similarity4
Regioni215 – 218G4By similarity4
Regioni253 – 255G5By similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O24310-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSSTAATT SSKLKLSNPP SLSHTFTASA SASVSNSTSF RPKLTLTRLS
60 70 80 90 100
SSFLNPSTIL HLTPSQRTNR PSSSPFTVRA ARGKFERKKP HLNIGTIGHV
110 120 130 140 150
DHGKTTLTAA LTMALACLGN SAPKKYDEID AAPEERARGI TINTATVEYE
160 170 180 190 200
TETRHYAHVD CPGHADYVKN MITGAAQMDG AILVVSGADG PMPQTKEHIL
210 220 230 240 250
LAKQVGVPSV VVFLNKQDQV DDEELLELVE LEVRELLSSY EFPGDDIPIV
260 270 280 290 300
SGSALLALEA LMANPTLKRG NNQWVDKIYQ LMDEVDKYIP IPQRQTELPF
310 320 330 340 350
LLAIEDVFSI TXRGTVATGR IERGLVKVGD VVDLVGLRET RNTTVTGVEM
360 370 380 390 400
FQKILDDAMA GDNVGLLLRG IQKIDIQRGM VLAKPGTITP HSKFSAIVYV
410 420 430 440 450
LKKEEGGRHS PFFAGYRPQF YMRTTDVTGK VTSIMNDKDE ESKMVMPGDR
460 470 480
VKIVVELIVP VAIEQGMRFA IREGGKTVGA GVIGAIIE
Length:488
Mass (Da):53,050
Last modified:January 1, 1998 - v1
Checksum:i8C8D694B846A4897
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14561 mRNA Translation: CAA74893.1
AY083613 Genomic DNA Translation: AAM01198.1
PIRiT06821

Similar proteinsi

Entry informationi

Entry nameiEFTU_PEA
AccessioniPrimary (citable) accession number: O24310
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 22, 2017
This is version 77 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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