UniProtKB - O23791 (BROM1_ANACO)
Protein
Fruit bromelain
Gene
N/A
Organism
Ananas comosus (Pineapple) (Ananas ananas)
Status
Functioni
Catalytic activityi
- Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-|-NHMec is a good synthetic substrate, but there is no action on Z-Arg-Arg-|-NHMec (cf. stem bromelain). EC:3.4.22.33
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 147 | By similarity | 1 | |
Active sitei | 279 | By similarity | 1 |
GO - Molecular functioni
- cysteine-type peptidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.33, 333 |
Protein family/group databases
MEROPSi | C01.028 |
Names & Taxonomyi
Protein namesi | |
Organismi | Ananas comosus (Pineapple) (Ananas ananas) |
Taxonomic identifieri | 4615 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › Liliopsida › Poales › Bromeliaceae › Bromelioideae › Ananas |
Pathology & Biotechi
Allergenic propertiesi
Causes an allergic reaction in human.
Keywords - Diseasei
AllergenProtein family/group databases
Allergomei | 3076, Ana c 2.0101 694, Ana c 2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | Sequence analysisAdd BLAST | 24 | |
PropeptideiPRO_0000045809 | 25 – 121 | By similarityAdd BLAST | 97 | |
ChainiPRO_0000045810 | 122 – 351 | Fruit bromelainAdd BLAST | 230 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 144 ↔ 184 | By similarity | ||
Disulfide bondi | 178 ↔ 217 | By similarity | ||
Disulfide bondi | 273 ↔ 325 | By similarity |
Keywords - PTMi
Disulfide bond, ZymogenFamily & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O23791-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASKVQLVFL FLFLCAMWAS PSAASRDEPN DPMMKRFEEW MAEYGRVYKD
60 70 80 90 100
DDEKMRRFQI FKNNVKHIET FNSRNENSYT LGINQFTDMT KSEFVAQYTG
110 120 130 140 150
VSLPLNIERE PVVSFDDVNI SAVPQSIDWR DYGAVNEVKN QNPCGSCWSF
160 170 180 190 200
AAIATVEGIY KIKTGYLVSL SEQEVLDCAV SYGCKGGWVN KAYDFIISNN
210 220 230 240 250
GVTTEENYPY LAYQGTCNAN SFPNSAYITG YSYVRRNDER SMMYAVSNQP
260 270 280 290 300
IAALIDASEN FQYYNGGVFS GPCGTSLNHA ITIIGYGQDS SGTKYWIVRN
310 320 330 340 350
SWGSSWGEGG YVRMARGVSS SSGVCGIAMA PLFPTLQSGA NAEVIKMVSE
T
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14059 mRNA Translation: BAA21849.1 |
PIRi | T10503 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D14059 mRNA Translation: BAA21849.1 |
PIRi | T10503 |
3D structure databases
SMRi | O23791 |
ModBasei | Search... |
Protein family/group databases
Allergomei | 3076, Ana c 2.0101 694, Ana c 2 |
MEROPSi | C01.028 |
Enzyme and pathway databases
BRENDAi | 3.4.22.33, 333 |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BROM1_ANACO | |
Accessioni | O23791Primary (citable) accession number: O23791 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2006 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 11, 2019 | |
This is version 65 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Documents
- Allergens
Nomenclature of allergens and list of entries - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries