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Protein

Proteasome subunit beta type-7-A

Gene

PBB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Threonine protease

Enzyme and pathway databases

ReactomeiR-ATH-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-ATH-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-ATH-349425 Autodegradation of the E3 ubiquitin ligase COP1
R-ATH-5632684 Hedgehog 'on' state
R-ATH-5687128 MAPK6/MAPK4 signaling
R-ATH-5689603 UCH proteinases
R-ATH-5689880 Ub-specific processing proteases
R-ATH-6798695 Neutrophil degranulation
R-ATH-68949 Orc1 removal from chromatin
R-ATH-69017 CDK-mediated phosphorylation and removal of Cdc6
R-ATH-69229 Ubiquitin-dependent degradation of Cyclin D1
R-ATH-8951664 Neddylation
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit beta type-7-A (EC:3.4.25.1)
Alternative name(s):
20S proteasome beta subunit B-1
Proteasome component FA
Proteasome component FB
Proteasome subunit beta type-2
Gene namesi
Name:PBB1
Synonyms:PRCFA, PRCFB
Ordered Locus Names:At3g27430
ORF Names:K1G2.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G27430
TAIRilocus:2086681 AT3G27430

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000428301 – 37Removed in mature formAdd BLAST37
ChainiPRO_000004283138 – 273Proteasome subunit beta type-7-AAdd BLAST236

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiO23710
PRIDEiO23710

Expressioni

Gene expression databases

ExpressionAtlasiO23710 baseline and differential
GenevisibleiO23710 AT

Interactioni

Subunit structurei

Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.2 Publications

Protein-protein interaction databases

BioGridi7694, 3 interactors
IntActiO23710, 1 interactor
STRINGi3702.AT3G27430.2

Structurei

3D structure databases

ProteinModelPortaliO23710
SMRiO23710
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0173 Eukaryota
COG0638 LUCA
HOGENOMiHOG000182856
InParanoidiO23710
KOiK02739
OMAiQTLFRYR
OrthoDBiEOG09360IM6
PhylomeDBiO23710

Family and domain databases

Gene3Di3.60.20.10, 1 hit
InterProiView protein in InterPro
IPR029055 Ntn_hydrolases_N
IPR000243 Pept_T1A_subB
IPR016050 Proteasome_bsu_CS
IPR001353 Proteasome_sua/b
IPR023333 Proteasome_suB-type
PfamiView protein in Pfam
PF00227 Proteasome, 1 hit
PRINTSiPR00141 PROTEASOME
SUPFAMiSSF56235 SSF56235, 1 hit
PROSITEiView protein in PROSITE
PS00854 PROTEASOME_BETA_1, 1 hit
PS51476 PROTEASOME_BETA_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.iShow all

Isoform 1 (identifier: O23710-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSQSTVDVPP KGGFSFDLCK RNDMLTQKGL KAPSFLKTGT TIVGLIFKDG
60 70 80 90 100
VILGADTRAT EGPIVADKNC EKIHYMAPNI YCCGAGTAAD TEAVTDMVSS
110 120 130 140 150
QLRLHRYQTG RDSRVITALT LLKKHLFSYQ GHVSAALVLG GVDITGPHLH
160 170 180 190 200
TIYPHGSTDT LPFATMGSGS LAAMSVFEAK YKEGLTRDEG IKLVAESICS
210 220 230 240 250
GIFNDLGSGS NVDICVITKG NKEYLRNYME PNPRTYVSSK GYSFTKKTEV
260 270
LLTKITPLLE RVEITEVGEA MEE
Length:273
Mass (Da):29,555
Last modified:November 22, 2005 - v2
Checksum:i4133891315E0915C
GO
Isoform 2 (identifier: O23710-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     249-267: EVLLTKITPLLERVEITEV → GSSHQNHPIAGASRNHRSW
     268-273: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:267
Mass (Da):28,812
Checksum:iE6B193FC30548281
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F4IWI5F4IWI5_ARATH
N-terminal nucleophile aminohydrola...
PBB1 At3g27430
267Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti27Q → P in CAA73621 (PubMed:9373170).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_018144249 – 267EVLLT…EITEV → GSSHQNHPIAGASRNHRSW in isoform 2. CuratedAdd BLAST19
Alternative sequenceiVSP_018145268 – 273Missing in isoform 2. Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13174 mRNA Translation: CAA73617.1
Y13178 mRNA Translation: CAA73621.1
AF043530 mRNA Translation: AAC32066.1
AB024028 Genomic DNA Translation: BAA95719.1
CP002686 Genomic DNA Translation: AEE77318.1
CP002686 Genomic DNA Translation: AEE77319.1
BT003339 mRNA Translation: AAO29958.1
BT006306 mRNA Translation: AAP13414.1
AY086464 mRNA Translation: AAM63467.1
PIRiT51977
RefSeqiNP_566818.1, NM_113658.3 [O23710-1]
NP_850641.1, NM_180310.1 [O23710-2]
UniGeneiAt.24382

Genome annotation databases

EnsemblPlantsiAT3G27430.1; AT3G27430.1; AT3G27430 [O23710-2]
AT3G27430.2; AT3G27430.2; AT3G27430 [O23710-1]
GeneIDi822364
GrameneiAT3G27430.1; AT3G27430.1; AT3G27430 [O23710-2]
AT3G27430.2; AT3G27430.2; AT3G27430 [O23710-1]
KEGGiath:AT3G27430

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPSB7A_ARATH
AccessioniPrimary (citable) accession number: O23710
Secondary accession number(s): O81152, Q3EAZ2, Q9T0L7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: September 12, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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