UniProtKB - O23627 (SYGM1_ARATH)
Glycine--tRNA ligase, mitochondrial 1
At1g29880
Functioni
Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis.
By similarityCatalytic activityi
- EC:6.1.1.141 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 296 | GlycineBy similarity | 1 | |
Binding sitei | 347 | GlycineBy similarity | 1 | |
Binding sitei | 582 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 328 – 330 | ATPBy similarity | 3 | |
Nucleotide bindingi | 339 – 340 | ATPBy similarity | 2 | |
Nucleotide bindingi | 454 – 455 | ATPBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glycine-tRNA ligase activity Source: UniProtKB
- protein dimerization activity Source: UniProtKB
- transferase activity Source: UniProtKB-KW
GO - Biological processi
- diadenosine tetraphosphate biosynthetic process Source: UniProtKB
- glycyl-tRNA aminoacylation Source: GO_Central
- mitochondrial glycyl-tRNA aminoacylation Source: GO_Central
Keywordsi
Molecular function | Aminoacyl-tRNA synthetase, Ligase, Transferase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 6.1.1.14, 399 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:At1g29880 ORF Names:F1N18.8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G29880 |
TAIRi | locus:2019327, AT1G29880 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Cytoplasm and Cytosol
- cytosol 1 Publication
Chloroplast
- chloroplast Source: TAIR
Cytosol
- cytosol Source: UniProtKB-SubCell
Endosome
- endosome Source: TAIR
Golgi apparatus
- Golgi apparatus Source: TAIR
- trans-Golgi network Source: TAIR
Mitochondrion
- mitochondrion Source: TAIR
Nucleus
- nucleus Source: TAIR
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 28 | MitochondrionSequence analysisAdd BLAST | 28 | |
ChainiPRO_0000035802 | 29 – 729 | Glycine--tRNA ligase, mitochondrial 1Add BLAST | 701 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Isoform Cytoplasmic (identifier: O23627-2) | |||||
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | O23627 |
PRIDEi | O23627 |
ProteomicsDBi | 233040 [O23627-1] |
PTM databases
iPTMneti | O23627 |
MetOSitei | O23627 |
SwissPalmi | O23627 |
Expressioni
Gene expression databases
ExpressionAtlasi | O23627, baseline and differential |
Genevisiblei | O23627, AT |
Interactioni
Subunit structurei
Homodimer.
By similarityGO - Molecular functioni
- protein dimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 25101, 11 interactors |
STRINGi | 3702.AT1G29880.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 50 – 106 | WHEP-TRSPROSITE-ProRule annotationAdd BLAST | 57 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 575 – 577 | Glycine bindingBy similarity | 3 |
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2298, Eukaryota |
HOGENOMi | CLU_015515_1_0_1 |
InParanoidi | O23627 |
OMAi | EPSYGID |
OrthoDBi | 1183820at2759 |
PhylomeDBi | O23627 |
Family and domain databases
CDDi | cd00774, GlyRS-like_core, 1 hit |
Gene3Di | 3.30.930.10, 1 hit 3.40.50.800, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR027031, Gly-tRNA_synthase/POLG2 IPR033731, GlyRS-like_core IPR009068, S15_NS1_RNA-bd IPR002315, tRNA-synt_gly IPR000738, WHEP-TRS_dom |
PANTHERi | PTHR10745, PTHR10745, 1 hit |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF00587, tRNA-synt_2b, 1 hit PF00458, WHEP-TRS, 1 hit |
PRINTSi | PR01043, TRNASYNTHGLY |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00389, glyS_dimeric, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative initiation. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRIFSTFVFH RRQQIFNLRQ FQTTTILRNP ISIAPIQIPM DATEQSLRQS
60 70 80 90 100
LSEKSSSVEA QGNAVRALKA SRAAKPEIDA AIEQLNKLKL EKSTVEKELQ
110 120 130 140 150
SIISSSGNGS LNREAFRKAV VNTLERRLFY IPSFKIYSGV AGLFDYGPPG
160 170 180 190 200
CAIKSNVLSF WRQHFILEEN MLEVDCPCVT PEVVLKASGH VDKFTDLMVK
210 220 230 240 250
DEKTGTCYRA DHLLKDYCTE KLEKDLTISA EKAAELKDVL AVMEDFSPEQ
260 270 280 290 300
LGAKIREYGI TAPDTKNPLS DPYPFNLMFQ TSIGPSGLIP GYMRPETAQG
310 320 330 340 350
IFVNFKDLYY YNGKKLPFAA AQIGQAFRNE ISPRQGLLRV REFTLAEIEH
360 370 380 390 400
FVDPENKSHP KFSDVAKLEF LMFPREEQMS GQSAKKLCLG EAVAKGTVNN
410 420 430 440 450
ETLGYFIGRV YLFLTRLGID KERLRFRQHL ANEMAHYAAD CWDAEIESSY
460 470 480 490 500
GWIECVGIAD RSAYDLRAHS DKSGTPLVAE EKFAEPKEVE KLVITPVKKE
510 520 530 540 550
LGLAFKGNQK NVVESLEAMN EEEAMEMKAT LESKGEVEFY VCTLKKSVNI
560 570 580 590 600
KKNMVSISKE KKKEHQRVFT PSVIEPSFGI GRIIYCLYEH CFSTRPSKAG
610 620 630 640 650
DEQLNLFRFP PLVAPIKCTV FPLVQNQQFE EVAKVISKEL ASVGISHKID
660 670 680 690 700
ITGTSIGKRY ARTDELGVPF AITVDSDTSV TIRERDSKDQ VRVTLKEAAS
710 720
VVSSVSEGKM TWQDVWATFP HHSSAAADE
The sequence of this isoform differs from the canonical sequence as follows:
1-39: Missing.
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 1 | M → T in AAM64494 (Ref. 5) Curated | 1 | |
Sequence conflicti | 82 | I → M in AAK92832 (PubMed:14593172).Curated | 1 | |
Sequence conflicti | 632 | V → A in AAM64494 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018906 | 1 – 39 | Missing in isoform Cytoplasmic. CuratedAdd BLAST | 39 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002062 mRNA Translation: CAA05162.1 AC008030 Genomic DNA Translation: AAG10608.1 CP002684 Genomic DNA Translation: AEE31145.1 AY050895 mRNA Translation: AAK92832.1 AY086930 mRNA Translation: AAM64494.1 Different initiation. |
PIRi | D86422 |
RefSeqi | NP_564337.1, NM_102728.3 [O23627-1] |
Genome annotation databases
EnsemblPlantsi | AT1G29880.1; AT1G29880.1; AT1G29880 [O23627-1] |
GeneIDi | 839866 |
Gramenei | AT1G29880.1; AT1G29880.1; AT1G29880 [O23627-1] |
KEGGi | ath:AT1G29880 |
Keywords - Coding sequence diversityi
Alternative initiationSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ002062 mRNA Translation: CAA05162.1 AC008030 Genomic DNA Translation: AAG10608.1 CP002684 Genomic DNA Translation: AEE31145.1 AY050895 mRNA Translation: AAK92832.1 AY086930 mRNA Translation: AAM64494.1 Different initiation. |
PIRi | D86422 |
RefSeqi | NP_564337.1, NM_102728.3 [O23627-1] |
3D structure databases
SMRi | O23627 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 25101, 11 interactors |
STRINGi | 3702.AT1G29880.1 |
PTM databases
iPTMneti | O23627 |
MetOSitei | O23627 |
SwissPalmi | O23627 |
Proteomic databases
PaxDbi | O23627 |
PRIDEi | O23627 |
ProteomicsDBi | 233040 [O23627-1] |
Genome annotation databases
EnsemblPlantsi | AT1G29880.1; AT1G29880.1; AT1G29880 [O23627-1] |
GeneIDi | 839866 |
Gramenei | AT1G29880.1; AT1G29880.1; AT1G29880 [O23627-1] |
KEGGi | ath:AT1G29880 |
Organism-specific databases
Araporti | AT1G29880 |
TAIRi | locus:2019327, AT1G29880 |
Phylogenomic databases
eggNOGi | KOG2298, Eukaryota |
HOGENOMi | CLU_015515_1_0_1 |
InParanoidi | O23627 |
OMAi | EPSYGID |
OrthoDBi | 1183820at2759 |
PhylomeDBi | O23627 |
Enzyme and pathway databases
BRENDAi | 6.1.1.14, 399 |
Miscellaneous databases
PROi | PR:O23627 |
Gene expression databases
ExpressionAtlasi | O23627, baseline and differential |
Genevisiblei | O23627, AT |
Family and domain databases
CDDi | cd00774, GlyRS-like_core, 1 hit |
Gene3Di | 3.30.930.10, 1 hit 3.40.50.800, 1 hit |
InterProi | View protein in InterPro IPR002314, aa-tRNA-synt_IIb IPR006195, aa-tRNA-synth_II IPR045864, aa-tRNA-synth_II/BPL/LPL IPR004154, Anticodon-bd IPR036621, Anticodon-bd_dom_sf IPR027031, Gly-tRNA_synthase/POLG2 IPR033731, GlyRS-like_core IPR009068, S15_NS1_RNA-bd IPR002315, tRNA-synt_gly IPR000738, WHEP-TRS_dom |
PANTHERi | PTHR10745, PTHR10745, 1 hit |
Pfami | View protein in Pfam PF03129, HGTP_anticodon, 1 hit PF00587, tRNA-synt_2b, 1 hit PF00458, WHEP-TRS, 1 hit |
PRINTSi | PR01043, TRNASYNTHGLY |
SMARTi | View protein in SMART SM00991, WHEP-TRS, 1 hit |
SUPFAMi | SSF47060, SSF47060, 1 hit SSF55681, SSF55681, 1 hit |
TIGRFAMsi | TIGR00389, glyS_dimeric, 1 hit |
PROSITEi | View protein in PROSITE PS50862, AA_TRNA_LIGASE_II, 1 hit PS00762, WHEP_TRS_1, 1 hit PS51185, WHEP_TRS_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SYGM1_ARATH | |
Accessioni | O23627Primary (citable) accession number: O23627 Secondary accession number(s): Q8LBY0, Q949T7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Aminoacyl-tRNA synthetases
List of aminoacyl-tRNA synthetase entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families