UniProtKB - O22932 (CIPKB_ARATH)
Protein
CBL-interacting serine/threonine-protein kinase 11
Gene
CIPK11
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Acts as a negative regulator of the plasma membrane proton pump AHA2 by preventing its interaction with 14-3-3 protein.1 Publication
Catalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 50 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 145 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 27 – 35 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein kinase activity Source: TAIR
- protein serine kinase activity Source: UniProtKB-EC
- protein threonine kinase activity Source: UniProtKB-EC
GO - Biological processi
- protein phosphorylation Source: TAIR
- response to pH Source: TAIR
- signal transduction Source: InterPro
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Magnesium, Manganese, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: CBL-interacting serine/threonine-protein kinase 11 (EC:2.7.11.1)Alternative name(s): SNF1-related kinase 3.22 SOS2-like protein kinase PKS5 SOS3-interacting protein 4 |
Gene namesi | Name:CIPK11 Synonyms:PKS5, SIP4, SnRK3.22 Ordered Locus Names:At2g30360 ORF Names:T9D9.17 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G30360 |
TAIRi | locus:2065781, AT2G30360 |
Pathology & Biotechi
Disruption phenotypei
Increased proton extrusion and resistance to high external pH.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000085875 | 1 – 435 | CBL-interacting serine/threonine-protein kinase 11Add BLAST | 435 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 167 | PhosphoserineBy similarity | 1 | |
Modified residuei | 181 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Autophosphorylated.
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O22932 |
PRIDEi | O22932 |
ProteomicsDBi | 246798 |
Expressioni
Tissue specificityi
Expressed in roots and stems, but barely detectable in flowers and siliques.2 Publications
Developmental stagei
Expressed in hypocotyls and roots upon germination. As seedlings mature, detected in the vascular tissue of both the stem and leaf.1 Publication
Inductioni
Up-regulated by salt, drought, abscisic acid and glucose, but not by cold.2 Publications
Gene expression databases
ExpressionAtlasi | O22932, differential |
Genevisiblei | O22932, AT |
Interactioni
Subunit structurei
Interacts with CBL1, CBL2, CBL3, CBL4/SOS3, and CBL5.
3 PublicationsBinary interactionsi
Hide detailsO22932
With | #Exp. | IntAct |
---|---|---|
AHA2 [P19456] | 3 | EBI-537638,EBI-2293350 |
CBL2 [Q8LAS7] | 5 | EBI-537638,EBI-485991 |
CBL9 [Q9LTB8] | 5 | EBI-537638,EBI-637381 |
MYB73 [O23160] | 3 | EBI-537638,EBI-25506855 |
Protein-protein interaction databases
BioGRIDi | 2935, 38 interactors |
IntActi | O22932, 9 interactors |
STRINGi | 3702.AT2G30360.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 277 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 257 | |
Domaini | 302 – 326 | NAFPROSITE-ProRule annotationAdd BLAST | 25 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 163 – 192 | Activation loopBy similarityAdd BLAST | 30 | |
Regioni | 333 – 362 | PPIBy similarityAdd BLAST | 30 |
Domaini
The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity
Sequence similaritiesi
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Curated
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
HOGENOMi | CLU_000288_59_0_1 |
InParanoidi | O22932 |
OMAi | KEEEYGF |
OrthoDBi | 1127668at2759 |
PhylomeDBi | O22932 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR018451, NAF/FISL_domain IPR004041, NAF_dom IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF03822, NAF, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50816, NAF, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
O22932-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPEIEIAAGS GDNNDALFGK YELGKLLGCG AFAKVFHARD RRTGQSVAVK
60 70 80 90 100
ILNKKKLLTN PALANNIKRE ISIMRRLSHP NIVKLHEVMA TKSKIFFAME
110 120 130 140 150
FVKGGELFNK ISKHGRLSED LSRRYFQQLI SAVGYCHARG VYHRDLKPEN
160 170 180 190 200
LLIDENGNLK VSDFGLSALT DQIRPDGLLH TLCGTPAYVA PEILSKKGYE
210 220 230 240 250
GAKVDVWSCG IVLFVLVAGY LPFNDPNVMN MYKKIYKGEY RFPRWMSPDL
260 270 280 290 300
KRFVSRLLDI NPETRITIDE ILKDPWFVRG GFKQIKFHDD EIEDQKVESS
310 320 330 340 350
LEAVKSLNAF DLISYSSGLD LSGLFAGCSN SSGESERFLS EKSPEMLAEE
360 370 380 390 400
VEGFAREENL RMKKKKEEEY GFEMEGQNGK FGIGICISRL NDLLVVVEAR
410 420 430
RRGGDGDCYK EMWNGKLRVQ LIRVCDQTSS TNAAI
Sequence cautioni
The sequence AAL16166 differs from that shown. Reason: Frameshift.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF295666 mRNA Translation: AAK16686.1 AF339146 mRNA Translation: AAK26844.1 AC002338 Genomic DNA Translation: AAC16938.1 CP002685 Genomic DNA Translation: AEC08375.1 AF370595 mRNA Translation: AAK43914.1 AF428398 mRNA Translation: AAL16166.1 Frameshift. BT002478 mRNA Translation: AAO00838.1 BT006582 mRNA Translation: AAP31926.1 |
PIRi | E84707 |
RefSeqi | NP_180595.1, NM_128589.2 |
Genome annotation databases
EnsemblPlantsi | AT2G30360.1; AT2G30360.1; AT2G30360 |
GeneIDi | 817586 |
Gramenei | AT2G30360.1; AT2G30360.1; AT2G30360 |
KEGGi | ath:AT2G30360 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF295666 mRNA Translation: AAK16686.1 AF339146 mRNA Translation: AAK26844.1 AC002338 Genomic DNA Translation: AAC16938.1 CP002685 Genomic DNA Translation: AEC08375.1 AF370595 mRNA Translation: AAK43914.1 AF428398 mRNA Translation: AAL16166.1 Frameshift. BT002478 mRNA Translation: AAO00838.1 BT006582 mRNA Translation: AAP31926.1 |
PIRi | E84707 |
RefSeqi | NP_180595.1, NM_128589.2 |
3D structure databases
SMRi | O22932 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2935, 38 interactors |
IntActi | O22932, 9 interactors |
STRINGi | 3702.AT2G30360.1 |
Proteomic databases
PaxDbi | O22932 |
PRIDEi | O22932 |
ProteomicsDBi | 246798 |
Genome annotation databases
EnsemblPlantsi | AT2G30360.1; AT2G30360.1; AT2G30360 |
GeneIDi | 817586 |
Gramenei | AT2G30360.1; AT2G30360.1; AT2G30360 |
KEGGi | ath:AT2G30360 |
Organism-specific databases
Araporti | AT2G30360 |
TAIRi | locus:2065781, AT2G30360 |
Phylogenomic databases
eggNOGi | KOG0583, Eukaryota |
HOGENOMi | CLU_000288_59_0_1 |
InParanoidi | O22932 |
OMAi | KEEEYGF |
OrthoDBi | 1127668at2759 |
PhylomeDBi | O22932 |
Miscellaneous databases
PROi | PR:O22932 |
Gene expression databases
ExpressionAtlasi | O22932, differential |
Genevisiblei | O22932, AT |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR018451, NAF/FISL_domain IPR004041, NAF_dom IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF03822, NAF, 1 hit PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS50816, NAF, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CIPKB_ARATH | |
Accessioni | O22932Primary (citable) accession number: O22932 Secondary accession number(s): Q7FYB7, Q944I6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 23, 2004 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families