UniProtKB - O22711 (PRX2D_ARATH)
Protein
Peroxiredoxin-2D
Gene
PRXIID
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides (By similarity). May be involved in intracellular redox signaling (Probable).By similarity1 Publication
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this 1-Cys peroxiredoxin, no C(R) is present and C(P) instead forms a disulfide with a cysteine from another protein or with a small thiol molecule.By similarity
Catalytic activityi
- EC:1.11.1.25By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 51 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- thioredoxin peroxidase activity Source: GO_Central
GO - Biological processi
- cell redox homeostasis Source: GO_Central
- cellular response to oxidative stress Source: GO_Central
- hydrogen peroxide catabolic process Source: GO_Central
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Enzyme and pathway databases
BioCyci | ARA:AT1G60740-MONOMER |
Protein family/group databases
PeroxiBasei | 4355, AtPrxII04 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxiredoxin-2D (EC:1.11.1.25By similarity)Alternative name(s): Glutaredoxin-dependent peroxiredoxinCurated Peroxiredoxin IID Thioredoxin peroxidase 2D |
Gene namesi | Name:PRXIID Ordered Locus Names:At1g60740 ORF Names:F8A5.25 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G60740 |
TAIRi | locus:2036531, AT1G60740 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Plasma Membrane
- plasma membrane Source: TAIR
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000056611 | 1 – 162 | Peroxiredoxin-2DAdd BLAST | 162 |
Proteomic databases
PaxDbi | O22711 |
PRIDEi | O22711 |
ProteomicsDBi | 226297 |
Expressioni
Tissue specificityi
Exclusively expressed in buds and flowers. Also detected in pollen.1 Publication
Gene expression databases
ExpressionAtlasi | O22711, baseline and differential |
Genevisiblei | O22711, AT |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 3702.AT1G60740.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 162 | ThioredoxinPROSITE-ProRule annotationAdd BLAST | 159 |
Sequence similaritiesi
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | KOG0541, Eukaryota |
HOGENOMi | CLU_072440_1_2_1 |
InParanoidi | O22711 |
OMAi | TPPCSSH |
OrthoDBi | 1281610at2759 |
PhylomeDBi | O22711 |
Family and domain databases
CDDi | cd03013, PRX5_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR037944, PRX5-like IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10430, PTHR10430, 1 hit |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O22711-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAPITVGDVV PDGTISFFDE NDQLQTVSVH SIAAGKKVIL FGVPGAFTPT
60 70 80 90 100
CSMSHVPGFI GKAEELKSKG IDEIICFSVN DPFVMKAWGK TYQENKHVKF
110 120 130 140 150
VADGSGEYTH LLGLELDLKD KGLGIRSRRF ALLLDNLKVT VANVENGGEF
160
TVSSAEDILK AL
Sequence cautioni
The sequence AAB71961 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 70 | G → V in AAM62996 (Ref. 3) Curated | 1 | |
Sequence conflicti | 93 | Q → T in AAM62996 (Ref. 3) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002292 Genomic DNA Translation: AAB71961.1 Sequence problems. CP002684 Genomic DNA Translation: AEE33727.1 AY085779 mRNA Translation: AAM62996.1 AK176203 mRNA Translation: BAD43966.1 |
PIRi | G96632 |
RefSeqi | NP_564763.1, NM_104757.4 |
Genome annotation databases
EnsemblPlantsi | AT1G60740.1; AT1G60740.1; AT1G60740 |
GeneIDi | 842368 |
Gramenei | AT1G60740.1; AT1G60740.1; AT1G60740 |
KEGGi | ath:AT1G60740 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002292 Genomic DNA Translation: AAB71961.1 Sequence problems. CP002684 Genomic DNA Translation: AEE33727.1 AY085779 mRNA Translation: AAM62996.1 AK176203 mRNA Translation: BAD43966.1 |
PIRi | G96632 |
RefSeqi | NP_564763.1, NM_104757.4 |
3D structure databases
SMRi | O22711 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G60740.1 |
Protein family/group databases
PeroxiBasei | 4355, AtPrxII04 |
Proteomic databases
PaxDbi | O22711 |
PRIDEi | O22711 |
ProteomicsDBi | 226297 |
Genome annotation databases
EnsemblPlantsi | AT1G60740.1; AT1G60740.1; AT1G60740 |
GeneIDi | 842368 |
Gramenei | AT1G60740.1; AT1G60740.1; AT1G60740 |
KEGGi | ath:AT1G60740 |
Organism-specific databases
Araporti | AT1G60740 |
TAIRi | locus:2036531, AT1G60740 |
Phylogenomic databases
eggNOGi | KOG0541, Eukaryota |
HOGENOMi | CLU_072440_1_2_1 |
InParanoidi | O22711 |
OMAi | TPPCSSH |
OrthoDBi | 1281610at2759 |
PhylomeDBi | O22711 |
Enzyme and pathway databases
BioCyci | ARA:AT1G60740-MONOMER |
Miscellaneous databases
PROi | PR:O22711 |
Gene expression databases
ExpressionAtlasi | O22711, baseline and differential |
Genevisiblei | O22711, AT |
Family and domain databases
CDDi | cd03013, PRX5_like, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR037944, PRX5-like IPR013740, Redoxin IPR036249, Thioredoxin-like_sf IPR013766, Thioredoxin_domain |
PANTHERi | PTHR10430, PTHR10430, 1 hit |
Pfami | View protein in Pfam PF08534, Redoxin, 1 hit |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS51352, THIOREDOXIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRX2D_ARATH | |
Accessioni | O22711Primary (citable) accession number: O22711 Secondary accession number(s): Q67ZB5, Q8LDV2, Q9XEX3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | December 20, 2005 | |
Last modified: | December 2, 2020 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families