UniProtKB - O22607 (MSI4_ARATH)
Protein
WD-40 repeat-containing protein MSI4
Gene
MSI4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of the flowering autonomous pathway which positively regulates flowering by promoting transcriptional repression of the flowering repressor FLC. May promote histone deacetylation at the FLC locus leading to the formation of repressive chromatin structures. Also negatively regulates cold-responsive genes.4 Publications
Miscellaneous
Binds zinc.
GO - Molecular functioni
- metal ion binding Source: TAIR
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- chromatin organization Source: UniProtKB-KW
- DNA repair Source: TAIR
- flower development Source: UniProtKB-KW
- response to UV-B Source: TAIR
Keywordsi
Molecular function | Chromatin regulator, Developmental protein, Repressor |
Biological process | Differentiation, Flowering, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: WD-40 repeat-containing protein MSI4Alternative name(s): Altered cold-responsive gene 1 protein |
Gene namesi | Name:MSI4 Synonyms:ACG1, FVE Ordered Locus Names:At2g19520 ORF Names:F3P11.12 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G19520 |
TAIRi | locus:2050372, AT2G19520 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytosol
- cytosol Source: TAIR
Mitochondrion
- mitochondrion Source: TAIR
Nucleus
Other locations
- Cul4-RING E3 ubiquitin ligase complex Source: TAIR
- cytoplasm Source: TAIR
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 222 | A → V in allele fve-1; loss of function allele that causes increased levels of FLC and late flowering. 1 Publication | 1 | |
Mutagenesisi | 241 | D → N in allele fve-2; loss of function allele that causes increased levels of FLC and late flowering. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051083 | 1 – 507 | WD-40 repeat-containing protein MSI4Add BLAST | 507 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | O22607 |
PRIDEi | O22607 |
ProteomicsDBi | 250785 |
PTM databases
iPTMneti | O22607 |
Expressioni
Tissue specificityi
Expressed in rosette leaves, cauline leaves, main stems and developing fruits. Expressed at higher levels in roots and flowers.1 Publication
Gene expression databases
ExpressionAtlasi | O22607, baseline and differential |
Genevisiblei | O22607, AT |
Interactioni
Subunit structurei
Interacts with AHL16 and HOS1.
2 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 1826, 25 interactors |
DIPi | DIP-59614N |
IntActi | O22607, 8 interactors |
STRINGi | 3702.AT2G19520.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 162 – 193 | WD 1Add BLAST | 32 | |
Repeati | 217 – 248 | WD 2Add BLAST | 32 | |
Repeati | 290 – 321 | WD 3Add BLAST | 32 | |
Repeati | 335 – 366 | WD 4Add BLAST | 32 | |
Repeati | 384 – 415 | WD 5Add BLAST | 32 | |
Repeati | 439 – 477 | WD 6Add BLAST | 39 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 308 – 323 | DWD boxAdd BLAST | 16 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 468 – 471 | Poly-Gly | 4 |
Domaini
The DWD box is required for interaction with DDB1A.By similarity
Sequence similaritiesi
Belongs to the WD repeat RBAP46/RBAP48/MSI1 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0264, Eukaryota |
HOGENOMi | CLU_020445_2_0_1 |
InParanoidi | O22607 |
OMAi | GHKAAWS |
OrthoDBi | 831322at2759 |
PhylomeDBi | O22607 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR022052, Histone-bd_RBBP4_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12265, CAF1C_H4-bd, 1 hit PF00400, WD40, 4 hits |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
O22607-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESDEAAAVS PQATTPSGGT GASGPKKRGR KPKTKEDSQT PSSQQQSDVK
60 70 80 90 100
MKESGKKTQQ SPSVDEKYSQ WKGLVPILYD WLANHNLVWP SLSCRWGPQL
110 120 130 140 150
EQATYKNRQR LYLSEQTDGS VPNTLVIANC EVVKPRVAAA EHISQFNEEA
160 170 180 190 200
RSPFVKKYKT IIHPGEVNRI RELPQNSKIV ATHTDSPDVL IWDVETQPNR
210 220 230 240 250
HAVLGAANSR PDLILTGHQD NAEFALAMCP TEPFVLSGGK DKSVVLWSIQ
260 270 280 290 300
DHITTIGTDS KSSGSIIKQT GEGTDKNESP TVGPRGVYHG HEDTVEDVAF
310 320 330 340 350
SPTSAQEFCS VGDDSCLILW DARTGTNPVT KVEKAHDADL HCVDWNPHDD
360 370 380 390 400
NLILTGSADN TVRLFDRRKL TANGVGSPIY KFEGHKAAVL CVQWSPDKSS
410 420 430 440 450
VFGSSAEDGL LNIWDYDRVS KKSDRAAKSP AGLFFQHAGH RDKVVDFHWN
460 470 480 490 500
ASDPWTIVSV SDDCETTGGG GTLQIWRMSD LIYRPEEEVV AELEKFKSHV
MTCASKP
Sequence cautioni
The sequence AAD10151 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence CAA85542 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 89 | W → L in CAA85542 (PubMed:8580968).Curated | 1 | |
Sequence conflicti | 126 | V → F in CAA85542 (PubMed:8580968).Curated | 1 | |
Sequence conflicti | 202 | A → P in AAD03340 (PubMed:9872415).Curated | 1 | |
Sequence conflicti | 270 | T → P in AAD03340 (PubMed:9872415).Curated | 1 | |
Sequence conflicti | 463 | D → A in CAA85543 (PubMed:8580968).Curated | 1 | |
Sequence conflicti | 489 | V → F in CAA85543 (PubMed:8580968).Curated | 1 | |
Sequence conflicti | 494 | E → A in AAD03340 (PubMed:9872415).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF498101 Genomic DNA Translation: AAP29474.1 AF498102 mRNA Translation: AAP29475.1 AY849994 Genomic DNA Translation: AAX51264.1 AC005917 Genomic DNA Translation: AAD10151.2 Different initiation. CP002685 Genomic DNA Translation: AEC06891.1 AY059799 mRNA Translation: AAL24281.1 AY057655 mRNA Translation: AAL15286.1 AY081447 mRNA Translation: AAM10009.1 AF028711 mRNA Translation: AAD03340.1 Z37286 mRNA Translation: CAA85542.1 Frameshift. Z37287 mRNA Translation: CAA85543.1 |
PIRi | G84577 |
RefSeqi | NP_565456.2, NM_127510.5 |
Genome annotation databases
EnsemblPlantsi | AT2G19520.1; AT2G19520.1; AT2G19520 |
GeneIDi | 816471 |
Gramenei | AT2G19520.1; AT2G19520.1; AT2G19520 |
KEGGi | ath:AT2G19520 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF498101 Genomic DNA Translation: AAP29474.1 AF498102 mRNA Translation: AAP29475.1 AY849994 Genomic DNA Translation: AAX51264.1 AC005917 Genomic DNA Translation: AAD10151.2 Different initiation. CP002685 Genomic DNA Translation: AEC06891.1 AY059799 mRNA Translation: AAL24281.1 AY057655 mRNA Translation: AAL15286.1 AY081447 mRNA Translation: AAM10009.1 AF028711 mRNA Translation: AAD03340.1 Z37286 mRNA Translation: CAA85542.1 Frameshift. Z37287 mRNA Translation: CAA85543.1 |
PIRi | G84577 |
RefSeqi | NP_565456.2, NM_127510.5 |
3D structure databases
SMRi | O22607 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1826, 25 interactors |
DIPi | DIP-59614N |
IntActi | O22607, 8 interactors |
STRINGi | 3702.AT2G19520.1 |
PTM databases
iPTMneti | O22607 |
Proteomic databases
PaxDbi | O22607 |
PRIDEi | O22607 |
ProteomicsDBi | 250785 |
Genome annotation databases
EnsemblPlantsi | AT2G19520.1; AT2G19520.1; AT2G19520 |
GeneIDi | 816471 |
Gramenei | AT2G19520.1; AT2G19520.1; AT2G19520 |
KEGGi | ath:AT2G19520 |
Organism-specific databases
Araporti | AT2G19520 |
TAIRi | locus:2050372, AT2G19520 |
Phylogenomic databases
eggNOGi | KOG0264, Eukaryota |
HOGENOMi | CLU_020445_2_0_1 |
InParanoidi | O22607 |
OMAi | GHKAAWS |
OrthoDBi | 831322at2759 |
PhylomeDBi | O22607 |
Miscellaneous databases
PROi | PR:O22607 |
Gene expression databases
ExpressionAtlasi | O22607, baseline and differential |
Genevisiblei | O22607, AT |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR022052, Histone-bd_RBBP4_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF12265, CAF1C_H4-bd, 1 hit PF00400, WD40, 4 hits |
SMARTi | View protein in SMART SM00320, WD40, 6 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS50082, WD_REPEATS_2, 3 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MSI4_ARATH | |
Accessioni | O22607Primary (citable) accession number: O22607 Secondary accession number(s): Q42322 Q9SLD1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | August 2, 2002 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families