UniProtKB - O22456 (SEP3_ARATH)
Protein
Developmental protein SEPALLATA 3
Gene
SEP3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Probable transcription factor active in inflorescence development and floral organogenesis. Functions with SEPALLATA1/AGL2 and SEPALLATA2/AGL4 to ensure proper development of petals, stamens and carpels and to prevent the indeterminate growth of the flower meristem. Interacts with APETALA1, AGAMOUS or APETALA3/PISTILLATA to form complexes, that could be involved in genes regulation during floral meristem development (PubMed:10821278, PubMed:11206550). Binds specifically to the CArG box DNA sequence 5'-CC (A/T)6 GG-3' (PubMed:16080001).3 Publications
GO - Molecular functioni
- DNA binding Source: TAIR
- DNA-binding transcription factor activity Source: TAIR
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- cell fate specification Source: TAIR
- flower development Source: TAIR
- mucilage extrusion from seed coat Source: TAIR
- plant ovule development Source: TAIR
- positive regulation of transcription by RNA polymerase II Source: InterPro
- regulation of transcription by RNA polymerase II Source: GO_Central
- seed coat development Source: TAIR
- specification of floral organ identity Source: TAIR
- specification of floral organ number Source: TAIR
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding |
Biological process | Differentiation, Flowering, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Developmental protein SEPALLATA 3Alternative name(s): Agamous-like MADS-box protein AGL9 |
Gene namesi | Name:SEP3 Synonyms:AGL9 Ordered Locus Names:At1g24260 ORF Names:F3I6.19 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G24260 |
TAIRi | locus:2032372, AT1G24260 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleus Source: TAIR
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Triple mutations in the SEP1, SEP2 and SEP3 genes result in the replacement of the stamens and petals by sepals and of the carpels by a new mutant flower with sepaloid organs.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000199485 | 1 – 251 | Developmental protein SEPALLATA 3Add BLAST | 251 |
Proteomic databases
PaxDbi | O22456 |
PRIDEi | O22456 |
ProteomicsDBi | 234508 [O22456-1] |
Expressioni
Developmental stagei
Expressed early during flower development within petals, stamens, and carpels.
Gene expression databases
ExpressionAtlasi | O22456, baseline and differential |
Genevisiblei | O22456, AT |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsO22456
GO - Molecular functioni
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 24278, 25 interactors |
DIPi | DIP-33800N |
IntActi | O22456, 23 interactors |
STRINGi | 3702.AT1G24260.2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O22456 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 57 | MADS-boxPROSITE-ProRule annotationAdd BLAST | 55 | |
Domaini | 91 – 181 | K-boxPROSITE-ProRule annotationAdd BLAST | 91 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 94 – 177 | Sequence analysisAdd BLAST | 84 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0014, Eukaryota |
InParanoidi | O22456 |
OMAi | NRLPNTE |
PhylomeDBi | O22456 |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR033896, MADS_MEF2-like IPR002487, TF_Kbox IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF01486, K-box, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS51297, K_BOX, 1 hit PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O22456-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGRGRVELKR IENKINRQVT FAKRRNGLLK KAYELSVLCD AEVALIIFSN
60 70 80 90 100
RGKLYEFCSS SSMLRTLERY QKCNYGAPEP NVPSREALAV ELSSQQEYLK
110 120 130 140 150
LKERYDALQR TQRNLLGEDL GPLSTKELES LERQLDSSLK QIRALRTQFM
160 170 180 190 200
LDQLNDLQSK ERMLTETNKT LRLRLADGYQ MPLQLNPNQE EVDHYGRHHH
210 220 230 240 250
QQQQHSQAFF QPLECEPILQ IGYQGQQDGM GAGPSVNNYM LGWLPYDTNS
I
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4I972 | F4I972_ARATH | K-box region and MADS-box transcrip... | SEP3 AGAMOUS-like 9, AGL9, SEPALLATA3, TRANSCRIPTION FACTOR AGL9, At1g24260 | 237 | Annotation score: |
Sequence cautioni
The sequence BAC43290 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008893 | 90 | Missing in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF015552 mRNA Translation: AAB67832.1 AC002396 Genomic DNA Translation: AAC00586.1 CP002684 Genomic DNA Translation: AEE30502.1 CP002684 Genomic DNA Translation: AEE30503.1 AY088272 mRNA Translation: AAM65812.1 AK118696 mRNA Translation: BAC43290.1 Different initiation. |
PIRi | T00656 |
RefSeqi | NP_564214.2, NM_102272.4 [O22456-1] NP_850953.1, NM_180622.3 [O22456-2] |
Genome annotation databases
EnsemblPlantsi | AT1G24260.1; AT1G24260.1; AT1G24260 [O22456-2] AT1G24260.2; AT1G24260.2; AT1G24260 [O22456-1] |
GeneIDi | 839040 |
Gramenei | AT1G24260.1; AT1G24260.1; AT1G24260 [O22456-2] AT1G24260.2; AT1G24260.2; AT1G24260 [O22456-1] |
KEGGi | ath:AT1G24260 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF015552 mRNA Translation: AAB67832.1 AC002396 Genomic DNA Translation: AAC00586.1 CP002684 Genomic DNA Translation: AEE30502.1 CP002684 Genomic DNA Translation: AEE30503.1 AY088272 mRNA Translation: AAM65812.1 AK118696 mRNA Translation: BAC43290.1 Different initiation. |
PIRi | T00656 |
RefSeqi | NP_564214.2, NM_102272.4 [O22456-1] NP_850953.1, NM_180622.3 [O22456-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4OX0 | X-ray | 2.49 | A/B/C/D | 75-178 | [»] | |
SMRi | O22456 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 24278, 25 interactors |
DIPi | DIP-33800N |
IntActi | O22456, 23 interactors |
STRINGi | 3702.AT1G24260.2 |
Proteomic databases
PaxDbi | O22456 |
PRIDEi | O22456 |
ProteomicsDBi | 234508 [O22456-1] |
Genome annotation databases
EnsemblPlantsi | AT1G24260.1; AT1G24260.1; AT1G24260 [O22456-2] AT1G24260.2; AT1G24260.2; AT1G24260 [O22456-1] |
GeneIDi | 839040 |
Gramenei | AT1G24260.1; AT1G24260.1; AT1G24260 [O22456-2] AT1G24260.2; AT1G24260.2; AT1G24260 [O22456-1] |
KEGGi | ath:AT1G24260 |
Organism-specific databases
Araporti | AT1G24260 |
TAIRi | locus:2032372, AT1G24260 |
Phylogenomic databases
eggNOGi | KOG0014, Eukaryota |
InParanoidi | O22456 |
OMAi | NRLPNTE |
PhylomeDBi | O22456 |
Miscellaneous databases
PROi | PR:O22456 |
Gene expression databases
ExpressionAtlasi | O22456, baseline and differential |
Genevisiblei | O22456, AT |
Family and domain databases
CDDi | cd00265, MADS_MEF2_like, 1 hit |
Gene3Di | 3.40.1810.10, 1 hit |
InterProi | View protein in InterPro IPR033896, MADS_MEF2-like IPR002487, TF_Kbox IPR002100, TF_MADSbox IPR036879, TF_MADSbox_sf |
Pfami | View protein in Pfam PF01486, K-box, 1 hit PF00319, SRF-TF, 1 hit |
PRINTSi | PR00404, MADSDOMAIN |
SMARTi | View protein in SMART SM00432, MADS, 1 hit |
SUPFAMi | SSF55455, SSF55455, 1 hit |
PROSITEi | View protein in PROSITE PS51297, K_BOX, 1 hit PS00350, MADS_BOX_1, 1 hit PS50066, MADS_BOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SEP3_ARATH | |
Accessioni | O22456Primary (citable) accession number: O22456 Secondary accession number(s): Q8GWQ4, Q8L9R5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 167 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references