UniProtKB - O22261 (NDT1_ARATH)
Nicotinamide adenine dinucleotide transporter 1, chloroplastic
NDT1
Functioni
Mediates the NAD+ import into chloroplast. Favors the NAD+(in)/ADP or AMP(out) antiport exchange, but is also able to catalyze a low unidirectional transport (uniport) of NAD+. Transports NAD+, nicotinic acid adenine dinucleotide, nicotinamide mononucleotide, nicotinic acid mononucleotide, FAD, FMN, TTP, TDP, TMP, UTP, UDP, UMP, CTP, CDP, CMP, GTP, GDP, GMP, 3'-AMP, ATP, ADP, AND AMP, has low transport activity with cAMP, pyrophosphate, NADH and alpha-NAD+, and has no activity with NADP+, NADPH, nicotinamide, nicotinic acid, adenosine, thiamine mono- or diphosphate, inorganic phosphate, CoA, folate, NaCl, malate, malonate, citrate, fumarate, aspartate, glutamate, S-adenosylmethionine, lysine, arginine, and ornithine.
2 PublicationsMiscellaneous
Activity regulationi
Kineticsi
- KM=0.24 mM for the NAD+/NAD+ exchange1 Publication
- Vmax=1.41 mmol/min/g enzyme for the NAD+/NAD+ exchange1 Publication
GO - Molecular functioni
- antiporter activity Source: UniProtKB-KW
- NAD transmembrane transporter activity Source: TAIR
GO - Biological processi
- NAD transmembrane transport Source: GO_Central
- NAD transport Source: TAIR
Keywordsi
Biological process | Antiport, Transport |
Protein family/group databases
TCDBi | 2.A.29.10.11, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Nicotinamide adenine dinucleotide transporter 1, chloroplasticShort name: AtNDT1 Alternative name(s): NAD(+) transporter 1 |
Gene namesi | Name:NDT1 Synonyms:NADT1 Ordered Locus Names:At2g47490 ORF Names:T30B22.21 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G47490 |
TAIRi | locus:2062002, AT2G47490 |
Subcellular locationi
Chloroplast
- chloroplast membrane Curated; Multi-pass membrane protein Curated
Chloroplast
- chloroplast membrane Source: TAIR
Mitochondrion
- mitochondrion Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 17 – 37 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 78 – 98 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Transmembranei | 117 – 137 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 171 – 191 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 216 – 232 | Helical; Name=5Sequence analysisAdd BLAST | 17 | |
Transmembranei | 271 – 293 | Helical; Name=6Sequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
Chloroplast, Membrane, PlastidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420695 | 1 – 312 | Nicotinamide adenine dinucleotide transporter 1, chloroplasticAdd BLAST | 312 |
Proteomic databases
PaxDbi | O22261 |
ProteomicsDBi | 251106 |
PTM databases
iPTMneti | O22261 |
Expressioni
Tissue specificityi
Gene expression databases
ExpressionAtlasi | O22261, baseline and differential |
Genevisiblei | O22261, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 11 – 103 | Solcar 1Add BLAST | 93 | |
Repeati | 111 – 199 | Solcar 2Add BLAST | 89 | |
Repeati | 211 – 299 | Solcar 3Add BLAST | 89 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0764, Eukaryota |
HOGENOMi | CLU_015166_6_4_1 |
InParanoidi | O22261 |
OMAi | HVHEGWR |
OrthoDBi | 1080385at2759 |
PhylomeDBi | O22261 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR044712, SLC25A32-like |
PANTHERi | PTHR45683, PTHR45683, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSANSHPPNS KNVLCNAAAG AAAGVVAATF VCPLDVIKTR FQVHGLPKLG
60 70 80 90 100
DANIKGSLIV GSLEQIFKRE GMRGLYRGLS PTVMALLSNW AIYFTMYDQL
110 120 130 140 150
KSFLCSNDHK LSVGANVLAA SGAGAATTIA TNPLWVVKTR LQTQGMRVGI
160 170 180 190 200
VPYKSTFSAL RRIAYEEGIR GLYSGLVPAL AGISHVAIQF PTYEMIKVYL
210 220 230 240 250
AKKGDKSVDN LNARDVAVAS SIAKIFASTL TYPHEVVRAR LQEQGHHSEK
260 270 280 290 300
RYSGVRDCIK KVFEKDGFPG FYRGCATNLL RTTPAAVITF TSFEMVHRFL
310
VTHIPSEQSS IL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ871011 mRNA Translation: CAI38582.1 FM211595 mRNA Translation: CAR70090.1 AC002535 Genomic DNA Translation: AAC62861.2 CP002685 Genomic DNA Translation: AEC10850.1 AF372957 mRNA Translation: AAK50096.1 AY074833 mRNA Translation: AAL69531.1 AK316807 mRNA Translation: BAH19521.1 AY084889 mRNA Translation: AAM61452.1 |
PIRi | T00435 |
RefSeqi | NP_566102.1, NM_130317.3 |
Genome annotation databases
EnsemblPlantsi | AT2G47490.1; AT2G47490.1; AT2G47490 |
GeneIDi | 819362 |
Gramenei | AT2G47490.1; AT2G47490.1; AT2G47490 |
KEGGi | ath:AT2G47490 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ871011 mRNA Translation: CAI38582.1 FM211595 mRNA Translation: CAR70090.1 AC002535 Genomic DNA Translation: AAC62861.2 CP002685 Genomic DNA Translation: AEC10850.1 AF372957 mRNA Translation: AAK50096.1 AY074833 mRNA Translation: AAL69531.1 AK316807 mRNA Translation: BAH19521.1 AY084889 mRNA Translation: AAM61452.1 |
PIRi | T00435 |
RefSeqi | NP_566102.1, NM_130317.3 |
3D structure databases
AlphaFoldDBi | O22261 |
SMRi | O22261 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G47490.1 |
Protein family/group databases
TCDBi | 2.A.29.10.11, the mitochondrial carrier (mc) family |
PTM databases
iPTMneti | O22261 |
Proteomic databases
PaxDbi | O22261 |
ProteomicsDBi | 251106 |
Genome annotation databases
EnsemblPlantsi | AT2G47490.1; AT2G47490.1; AT2G47490 |
GeneIDi | 819362 |
Gramenei | AT2G47490.1; AT2G47490.1; AT2G47490 |
KEGGi | ath:AT2G47490 |
Organism-specific databases
Araporti | AT2G47490 |
TAIRi | locus:2062002, AT2G47490 |
Phylogenomic databases
eggNOGi | KOG0764, Eukaryota |
HOGENOMi | CLU_015166_6_4_1 |
InParanoidi | O22261 |
OMAi | HVHEGWR |
OrthoDBi | 1080385at2759 |
PhylomeDBi | O22261 |
Miscellaneous databases
PROi | PR:O22261 |
Gene expression databases
ExpressionAtlasi | O22261, baseline and differential |
Genevisiblei | O22261, AT |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002067, Mit_carrier IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf IPR044712, SLC25A32-like |
PANTHERi | PTHR45683, PTHR45683, 1 hit |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00926, MITOCARRIER |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | NDT1_ARATH | |
Accessioni | O22261Primary (citable) accession number: O22261 Secondary accession number(s): Q94JT4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2013 |
Last sequence update: | June 1, 2002 | |
Last modified: | May 25, 2022 | |
This is version 147 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families