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UniProtKB - O22218 (ACA4_ARATH)
Protein
Calcium-transporting ATPase 4, plasma membrane-type
Gene
ACA4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.
Catalytic activityi
- EC:7.2.2.10
Activity regulationi
Activated by calmodulin.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 451 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 749 | MagnesiumBy similarity | 1 | |
Metal bindingi | 753 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled cation transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: TAIR
- metal ion binding Source: UniProtKB-KW
- P-type calcium transporter activity Source: TAIR
GO - Biological processi
- anion homeostasis Source: TAIR
- defense response to bacterium Source: TAIR
- negative regulation of programmed cell death Source: TAIR
- response to nematode Source: TAIR
Keywordsi
Molecular function | Calmodulin-binding, Translocase |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G41560-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium-transporting ATPase 4, plasma membrane-type (EC:7.2.2.10)Alternative name(s): Ca(2+)-ATPase isoform 4 |
Gene namesi | Name:ACA4 Ordered Locus Names:At2g41560 ORF Names:T32G6.8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G41560 |
TAIRi | locus:2062673, AT2G41560 |
Subcellular locationi
Vacuole
- Vacuole membrane 2 Publications; Multi-pass membrane protein Sequence analysis
Note: Tonoplast. Small vacuoles.1 Publication
Chloroplast
- chloroplast Source: TAIR
Cytosol
- cytosol Source: TAIR
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Vacuole
- plant-type vacuole Source: TAIR
- plant-type vacuole membrane Source: TAIR
- vacuolar membrane Source: TAIR
- vacuole Source: TAIR
Other locations
- intracellular membrane-bounded organelle Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 157 | CytoplasmicSequence analysisAdd BLAST | 157 | |
Transmembranei | 158 – 178 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 179 – 196 | LumenalSequence analysisAdd BLAST | 18 | |
Transmembranei | 197 – 217 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 218 – 345 | CytoplasmicSequence analysisAdd BLAST | 128 | |
Transmembranei | 346 – 365 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 366 – 395 | LumenalSequence analysisAdd BLAST | 30 | |
Transmembranei | 396 – 413 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 414 – 804 | CytoplasmicSequence analysisAdd BLAST | 391 | |
Transmembranei | 805 – 823 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 824 – 834 | LumenalSequence analysisAdd BLAST | 11 | |
Transmembranei | 835 – 855 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 856 – 875 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 876 – 898 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 899 – 910 | LumenalSequence analysisAdd BLAST | 12 | |
Transmembranei | 911 – 932 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 933 – 950 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 951 – 972 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 973 – 982 | LumenalSequence analysis | 10 | |
Transmembranei | 983 – 1004 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 1005 – 1030 | CytoplasmicSequence analysisAdd BLAST | 26 |
Keywords - Cellular componenti
Membrane, VacuolePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046412 | 1 – 1030 | Calcium-transporting ATPase 4, plasma membrane-typeAdd BLAST | 1030 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O22218 |
PRIDEi | O22218 |
ProteomicsDBi | 244534 |
PTM databases
iPTMneti | O22218 |
Expressioni
Gene expression databases
ExpressionAtlasi | O22218, baseline and differential |
Genevisiblei | O22218, AT |
Interactioni
GO - Molecular functioni
- calmodulin binding Source: TAIR
Protein-protein interaction databases
BioGRIDi | 4091, 1 interactor |
STRINGi | 3702.AT2G41560.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 19 – 30 | Interaction with calmodulinAdd BLAST | 12 |
Domaini
The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0204, Eukaryota |
HOGENOMi | CLU_002360_9_0_1 |
InParanoidi | O22218 |
OrthoDBi | 115892at2759 |
PhylomeDBi | O22218 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR024750, Ca_ATPase_N_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006408, P-type_ATPase_IIB IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF12515, CaATP_NAI, 1 hit PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01517, ATPase-IIB_Ca, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O22218-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE
60 70 80 90 100
NKKHQIQEKI RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL
110 120 130 140 150
ASMVRKNDTK SLAQKGGVEE LAKKVSVSLS EGIRSSEVPI REKIFGENRY
160 170 180 190 200
TEKPARSFLM FVWEALHDIT LIILMVCAVV SIGVGVATEG FPRGMYDGTG
210 220 230 240 250
ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD GSRQEISIHD
260 270 280 290 300
LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL
310 320 330 340 350
LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT
360 370 380 390 400
IIGKIGLSFA VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV
410 420 430 440 450
TIIVVAVPEG LPLAVTLSLA FAMKKLMSDR ALVRHLAACE TMGSSTCICT
460 470 480 490 500
DKTGTLTTNH MVVNKVWICD KVQERQEGSK ESFELELSEE VQSTLLQGIF
510 520 530 540 550
QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ RKEHKILKIE
560 570 580 590 600
PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE
610 620 630 640 650
ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK
660 670 680 690 700
DPVRPGVREA VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE
710 720 730 740 750
GSEFRDLSPH EMRAIIPKIQ VMARSLPLDK HTLVSNLRKI GEVVAVTGDG
760 770 780 790 800
TNDAPALHEA DIGLAMGIAG TEVAKENADV IIMDDNFKTI VNVARWGRAV
810 820 830 840 850
YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL WVNMIMDTLG
860 870 880 890 900
ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF
910 920 930 940 950
AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN
960 970 980 990 1000
SWVFTWVMTV TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA
1010 1020 1030
VILKCVPVES RHHHDGYDLL PSGPSSSNSA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AXT8 | A0A1P8AXT8_ARATH | Calcium-transporting ATPase | ACA4 ''autoinhibited Ca(2+)-ATPase, autoinhibited Ca(2+)-ATPase, isoform 4, isoform 4'', At2g41560 | 928 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF200739 mRNA Translation: AAG35585.1 AC002510 Genomic DNA Translation: AAB84338.1 CP002685 Genomic DNA Translation: AEC10000.1 |
PIRi | T00812 |
RefSeqi | NP_181687.1, NM_129719.4 |
Genome annotation databases
EnsemblPlantsi | AT2G41560.1; AT2G41560.1; AT2G41560 |
GeneIDi | 818754 |
Gramenei | AT2G41560.1; AT2G41560.1; AT2G41560 |
KEGGi | ath:AT2G41560 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF200739 mRNA Translation: AAG35585.1 AC002510 Genomic DNA Translation: AAB84338.1 CP002685 Genomic DNA Translation: AEC10000.1 |
PIRi | T00812 |
RefSeqi | NP_181687.1, NM_129719.4 |
3D structure databases
SMRi | O22218 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4091, 1 interactor |
STRINGi | 3702.AT2G41560.1 |
PTM databases
iPTMneti | O22218 |
Proteomic databases
PaxDbi | O22218 |
PRIDEi | O22218 |
ProteomicsDBi | 244534 |
Genome annotation databases
EnsemblPlantsi | AT2G41560.1; AT2G41560.1; AT2G41560 |
GeneIDi | 818754 |
Gramenei | AT2G41560.1; AT2G41560.1; AT2G41560 |
KEGGi | ath:AT2G41560 |
Organism-specific databases
Araporti | AT2G41560 |
TAIRi | locus:2062673, AT2G41560 |
Phylogenomic databases
eggNOGi | KOG0204, Eukaryota |
HOGENOMi | CLU_002360_9_0_1 |
InParanoidi | O22218 |
OrthoDBi | 115892at2759 |
PhylomeDBi | O22218 |
Enzyme and pathway databases
BioCyci | ARA:AT2G41560-MONOMER |
Miscellaneous databases
PROi | PR:O22218 |
Gene expression databases
ExpressionAtlasi | O22218, baseline and differential |
Genevisiblei | O22218, AT |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR024750, Ca_ATPase_N_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006408, P-type_ATPase_IIB IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF12515, CaATP_NAI, 1 hit PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01517, ATPase-IIB_Ca, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACA4_ARATH | |
Accessioni | O22218Primary (citable) accession number: O22218 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families