ACA4

Functionⁱ

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.

Catalytic activityⁱ

ATP + H₂O + Ca²⁺(Side 1) = ADP + phosphate + Ca²⁺(Side 2).

Activity regulationⁱ

Activated by calmodulin.

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	451	4-aspartylphosphate intermediateBy similarity	1
Metal bindingⁱ	749	MagnesiumBy similarity	1
Metal bindingⁱ	753	MagnesiumBy similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
calcium-transporting ATPase activity
Source: TAIR
Inferred from sequence or structural similarityⁱ
- 12805592
calmodulin binding
Source: TAIR
Inferred from direct assayⁱ
- 11115896
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Calmodulin-binding, Hydrolase
Biological process	Calcium transport, Ion transport, Transport
Ligand	ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	ARA:AT2G41560-MONOMER
Reactomeⁱ	R-ATH-418359 Reduction of cytosolic Ca++ levels R-ATH-5578775 Ion homeostasis R-ATH-936837 Ion transport by P-type ATPases

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Calcium-transporting ATPase 4, plasma membrane-type (EC:3.6.3.8) Alternative name(s): Ca(2+)-ATPase isoform 4
Gene namesⁱ	Name:ACA4 Ordered Locus Names:At2g41560 ORF Names:T32G6.8
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 2

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT2G41560
TAIRⁱ	locus:2062673 AT2G41560

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 157	CytoplasmicSequence analysisAdd BLAST	157
Transmembraneⁱ	158 – 178	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	179 – 196	LumenalSequence analysisAdd BLAST	18
Transmembraneⁱ	197 – 217	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	218 – 345	CytoplasmicSequence analysisAdd BLAST	128
Transmembraneⁱ	346 – 365	HelicalSequence analysisAdd BLAST	20
Topological domainⁱ	366 – 395	LumenalSequence analysisAdd BLAST	30
Transmembraneⁱ	396 – 413	HelicalSequence analysisAdd BLAST	18
Topological domainⁱ	414 – 804	CytoplasmicSequence analysisAdd BLAST	391
Transmembraneⁱ	805 – 823	HelicalSequence analysisAdd BLAST	19
Topological domainⁱ	824 – 834	LumenalSequence analysisAdd BLAST	11
Transmembraneⁱ	835 – 855	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	856 – 875	CytoplasmicSequence analysisAdd BLAST	20
Transmembraneⁱ	876 – 898	HelicalSequence analysisAdd BLAST	23
Topological domainⁱ	899 – 910	LumenalSequence analysisAdd BLAST	12
Transmembraneⁱ	911 – 932	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	933 – 950	CytoplasmicSequence analysisAdd BLAST	18
Transmembraneⁱ	951 – 972	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	973 – 982	LumenalSequence analysis	10
Transmembraneⁱ	983 – 1004	HelicalSequence analysisAdd BLAST	22
Topological domainⁱ	1005 – 1030	CytoplasmicSequence analysisAdd BLAST	26

Keywords - Cellular componentⁱ

Membrane, Vacuole

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000046412	1 – 1030	Calcium-transporting ATPase 4, plasma membrane-typeAdd BLAST		1030

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	28	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	O22218
PRIDEⁱ	O22218

PTM databases

iPTMnetⁱ	O22218

iPTMnetⁱ

O22218

Expressionⁱ

Gene expression databases

ExpressionAtlasⁱ	O22218 baseline and differential
Genevisibleⁱ	O22218 AT

Interactionⁱ

GO - Molecular functionⁱ

calmodulin binding
Source: TAIR
Inferred from direct assayⁱ
- 11115896

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	4091, 1 interactor
STRINGⁱ	3702.AT2G41560.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O22218
SMRⁱ	O22218
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	19 – 30	Interaction with calmodulinAdd BLAST		12

Domainⁱ

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

Sequence similaritiesⁱ

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0204 Eukaryota ENOG410XNNC LUCA
HOGENOMⁱ	HOG000265623
InParanoidⁱ	O22218
KEGG Orthology (KO) More...KOⁱ	K01537
OMAⁱ	SNPIFCT
Database of Orthologous Groups More...OrthoDBⁱ	EOG09360106
PhylomeDBⁱ	O22218

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 2 hits
InterProⁱ	View protein in InterPro IPR006068 ATPase_P-typ_cation-transptr_C IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR024750 Ca_ATPase_N_dom IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006408 P-type_ATPase_IIB IPR001757 P_typ_ATPase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF12515 CaATP_NAI, 1 hit PF00689 Cation_ATPase_C, 1 hit PF00690 Cation_ATPase_N, 1 hit
PRINTSⁱ	PR00120 HATPASE
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 2 hits SSF81653 SSF81653, 1 hit SSF81660 SSF81660, 1 hit SSF81665 SSF81665, 3 hits
TIGRFAMsⁱ	TIGR01517 ATPase-IIB_Ca, 1 hit TIGR01494 ATPase_P-type, 3 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

O22218-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE 
        60         70         80         90        100
NKKHQIQEKI RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL 
       110        120        130        140        150
ASMVRKNDTK SLAQKGGVEE LAKKVSVSLS EGIRSSEVPI REKIFGENRY 
       160        170        180        190        200
TEKPARSFLM FVWEALHDIT LIILMVCAVV SIGVGVATEG FPRGMYDGTG 
       210        220        230        240        250
ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD GSRQEISIHD 
       260        270        280        290        300
LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL 
       310        320        330        340        350
LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT 
       360        370        380        390        400
IIGKIGLSFA VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV 
       410        420        430        440        450
TIIVVAVPEG LPLAVTLSLA FAMKKLMSDR ALVRHLAACE TMGSSTCICT 
       460        470        480        490        500
DKTGTLTTNH MVVNKVWICD KVQERQEGSK ESFELELSEE VQSTLLQGIF 
       510        520        530        540        550
QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ RKEHKILKIE 
       560        570        580        590        600
PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE 
       610        620        630        640        650
ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK 
       660        670        680        690        700
DPVRPGVREA VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE 
       710        720        730        740        750
GSEFRDLSPH EMRAIIPKIQ VMARSLPLDK HTLVSNLRKI GEVVAVTGDG 
       760        770        780        790        800
TNDAPALHEA DIGLAMGIAG TEVAKENADV IIMDDNFKTI VNVARWGRAV 
       810        820        830        840        850
YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL WVNMIMDTLG 
       860        870        880        890        900
ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF 
       910        920        930        940        950
AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN 
       960        970        980        990       1000
SWVFTWVMTV TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA 
      1010       1020       1030
VILKCVPVES RHHHDGYDLL PSGPSSSNSA

Length:1,030

Mass (Da):112,749

Last modified:January 1, 1998 - v1

Checksum:ⁱBD31D923E6AE2D13

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A1P8AXT8	A0A1P8AXT8_ARATH		Calcium-transporting ATPase Calcium-transporting ATPase, EC 3.6.3.8	ACA4 ''autoinhibited Ca(2+)-ATPase, autoinhibited Ca(2+)-ATPase, isoform 4, isoform 4'', At2g41560	928	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF200739 mRNA Translation: AAG35585.1 AC002510 Genomic DNA Translation: AAB84338.1 CP002685 Genomic DNA Translation: AEC10000.1
PIRⁱ	T00812
NCBI Reference Sequences More...RefSeqⁱ	NP_181687.1, NM_129719.4
UniGeneⁱ	At.24960 At.67009

Genome annotation databases

EnsemblPlantsⁱ	AT2G41560.1; AT2G41560.1; AT2G41560
GeneIDⁱ	818754
Grameneⁱ	AT2G41560.1; AT2G41560.1; AT2G41560
KEGGⁱ	ath:AT2G41560

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O22218	Calcium-transporting ATPase		ARATH		928	UniRef100_O22218

Protein	Similar proteins		Species	Score	Length	Source
O22218	Calcium-transporting ATPase		ARATH		928	UniRef90_O22218
Calcium-transporting ATPase		Brassica oleracea var. oleracea		1082
Calcium-transporting ATPase		BRARP		1032
Calcium-transporting ATPase		ARAAL		1031
Calcium-transporting ATPase		ARALL		1030
+14

Protein	Similar proteins		Species	Score	Length	Source
O22218	Probable calcium-transporting ATPase 6, plasma membrane-type		ORYSJ		1021	UniRef50_O22218
Calcium-transporting ATPase		ARATH		928
Calcium-transporting ATPase		Brassica oleracea var. oleracea		1082
Calcium-transporting ATPase		BRARP		1032
Calcium-transporting ATPase		ARAAL		1031
+112

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF200739 mRNA Translation: AAG35585.1 AC002510 Genomic DNA Translation: AAB84338.1 CP002685 Genomic DNA Translation: AEC10000.1
PIRⁱ	T00812
RefSeqⁱ	NP_181687.1, NM_129719.4
UniGeneⁱ	At.24960 At.67009

3D structure databases

ProteinModelPortalⁱ	O22218
SMRⁱ	O22218
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4091, 1 interactor
STRINGⁱ	3702.AT2G41560.1

PTM databases

iPTMnetⁱ	O22218

iPTMnetⁱ

O22218

Proteomic databases

PaxDbⁱ	O22218
PRIDEⁱ	O22218

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT2G41560.1; AT2G41560.1; AT2G41560
GeneIDⁱ	818754
Grameneⁱ	AT2G41560.1; AT2G41560.1; AT2G41560
KEGGⁱ	ath:AT2G41560

Organism-specific databases

Araportⁱ	AT2G41560
TAIRⁱ	locus:2062673 AT2G41560

Phylogenomic databases

eggNOGⁱ	KOG0204 Eukaryota ENOG410XNNC LUCA
HOGENOMⁱ	HOG000265623
InParanoidⁱ	O22218
KOⁱ	K01537
OMAⁱ	SNPIFCT
OrthoDBⁱ	EOG09360106
PhylomeDBⁱ	O22218

Enzyme and pathway databases

BioCycⁱ	ARA:AT2G41560-MONOMER
Reactomeⁱ	R-ATH-418359 Reduction of cytosolic Ca++ levels R-ATH-5578775 Ion homeostasis R-ATH-936837 Ion transport by P-type ATPases

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O22218

PROⁱ

PR:O22218

Gene expression databases

ExpressionAtlasⁱ	O22218 baseline and differential
Genevisibleⁱ	O22218 AT

Family and domain databases

Gene3Dⁱ	3.40.1110.10, 1 hit 3.40.50.1000, 2 hits
InterProⁱ	View protein in InterPro IPR006068 ATPase_P-typ_cation-transptr_C IPR004014 ATPase_P-typ_cation-transptr_N IPR023299 ATPase_P-typ_cyto_dom_N IPR018303 ATPase_P-typ_P_site IPR023298 ATPase_P-typ_TM_dom_sf IPR008250 ATPase_P-typ_transduc_dom_A_sf IPR024750 Ca_ATPase_N_dom IPR036412 HAD-like_sf IPR023214 HAD_sf IPR006408 P-type_ATPase_IIB IPR001757 P_typ_ATPase
Pfamⁱ	View protein in Pfam PF12515 CaATP_NAI, 1 hit PF00689 Cation_ATPase_C, 1 hit PF00690 Cation_ATPase_N, 1 hit
PRINTSⁱ	PR00120 HATPASE
SMARTⁱ	View protein in SMART SM00831 Cation_ATPase_N, 1 hit
SUPFAMⁱ	SSF56784 SSF56784, 2 hits SSF81653 SSF81653, 1 hit SSF81660 SSF81660, 1 hit SSF81665 SSF81665, 3 hits
TIGRFAMsⁱ	TIGR01517 ATPase-IIB_Ca, 1 hit TIGR01494 ATPase_P-type, 3 hits
PROSITEⁱ	View protein in PROSITE PS00154 ATPASE_E1_E2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ACA4_ARATH
Accessionⁱ	O22218Primary (citable) accession number: O22218
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
	Last sequence update:	January 1, 1998
	Last modified:	September 12, 2018
	This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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Supporting data

UniProtKB - O22218 (ACA4_ARATH)

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Calcium-transporting ATPase 4, plasma membrane-type

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Vacuole

Chloroplast

Plasma Membrane

Vacuole

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ