UniProtKB - O22173 (PABP4_ARATH)
Protein
Polyadenylate-binding protein 4
Gene
PAB4
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation (By similarity). During infection with potyvirus TuMV, acts as a potential integral component of the viral replicase complex that could play an important role in the regulation of potyviral RNA-dependent RNA polymerase (RdRp) (By similarity).By similarity
Miscellaneous
A.thaliana contains 8 PABP genes.
GO - Molecular functioni
- mRNA 3'-UTR binding Source: GO_Central
- mRNA binding Source: TAIR
- poly(A) binding Source: GO_Central
- poly(U) RNA binding Source: GO_Central
- RNA binding Source: GO_Central
GO - Biological processi
- regulation of translation Source: UniProtKB-KW
- response to cadmium ion Source: TAIR
- viral process Source: UniProtKB-KW
Keywordsi
Molecular function | RNA-binding |
Biological process | Host-virus interaction, Translation regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Polyadenylate-binding protein 4Short name: PABP-4 Short name: Poly(A)-binding protein 4 |
Gene namesi | Name:PAB4 Ordered Locus Names:At2g23350 ORF Names:T20D16.2 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G23350 |
TAIRi | locus:2058573, AT2G23350 |
Subcellular locationi
Cytosol
- cytosol Source: TAIR
Nucleus
- nucleus Source: GO_Central
Vacuole
- vacuole Source: TAIR
Other locations
- ribonucleoprotein complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Pab2 and pab4 double mutants show significant growth and development defects and more resistance to Turnip mosaic virus (TuMV).1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422643 | 1 – 662 | Polyadenylate-binding protein 4Add BLAST | 662 |
Proteomic databases
PaxDbi | O22173 |
PRIDEi | O22173 |
ProMEXi | O22173 |
ProteomicsDBi | 248630 |
PTM databases
iPTMneti | O22173 |
MetOSitei | O22173 |
Expressioni
Inductioni
By potyvirus TuMV infection.1 Publication
Gene expression databases
ExpressionAtlasi | O22173, baseline and differential |
Genevisiblei | O22173, AT |
Interactioni
Subunit structurei
Interacts with ERD15/CID1.
Interacts with Turnip mosaic virus (TuMV) VPg-Pro.
2 PublicationsProtein-protein interaction databases
BioGRIDi | 2219, 5 interactors |
IntActi | O22173, 4 interactors |
STRINGi | 3702.AT2G23350.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 46 – 124 | RRM 1PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 134 – 211 | RRM 2PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 225 – 302 | RRM 3PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 328 – 405 | RRM 4PROSITE-ProRule annotationAdd BLAST | 78 | |
Domaini | 558 – 635 | PABCPROSITE-ProRule annotationAdd BLAST | 78 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 12 – 15 | Poly-Pro | 4 | |
Compositional biasi | 30 – 39 | Poly-Gly | 10 |
Sequence similaritiesi
Belongs to the polyadenylate-binding protein type-1 family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0123, Eukaryota |
HOGENOMi | CLU_012062_22_4_1 |
InParanoidi | O22173 |
OMAi | MIRITYS |
OrthoDBi | 1027234at2759 |
PhylomeDBi | O22173 |
Family and domain databases
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR036053, PABP-dom IPR006515, PABP_1234 IPR002004, PABP_HYD IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk |
Pfami | View protein in Pfam PF00658, PABP, 1 hit PF00076, RRM_1, 4 hits |
SMARTi | View protein in SMART SM00517, PolyA, 1 hit SM00360, RRM, 4 hits SM00361, RRM_1, 4 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits SSF63570, SSF63570, 1 hit |
TIGRFAMsi | TIGR01628, PABP-1234, 1 hit |
PROSITEi | View protein in PROSITE PS51309, PABC, 1 hit PS50102, RRM, 4 hits |
i Sequence
Sequence statusi: Complete.
O22173-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAQVQAPSSH SPPPPAVVND GAATASATPG IGVGGGGDGV THGALCSLYV
60 70 80 90 100
GDLDFNVTDS QLYDYFTEVC QVVSVRVCRD AATNTSLGYG YVNYSNTDDA
110 120 130 140 150
EKAMQKLNYS YLNGKMIRIT YSSRDSSARR SGVGNLFVKN LDKSVDNKTL
160 170 180 190 200
HEAFSGCGTI VSCKVATDHM GQSRGYGFVQ FDTEDSAKNA IEKLNGKVLN
210 220 230 240 250
DKQIFVGPFL RKEERESAAD KMKFTNVYVK NLSEATTDDE LKTTFGQYGS
260 270 280 290 300
ISSAVVMRDG DGKSRCFGFV NFENPEDAAR AVEALNGKKF DDKEWYVGKA
310 320 330 340 350
QKKSERELEL SRRYEQGSSD GGNKFDGLNL YVKNLDDTVT DEKLRELFAE
360 370 380 390 400
FGTITSCKVM RDPSGTSKGS GFVAFSAASE ASRVLNEMNG KMVGGKPLYV
410 420 430 440 450
ALAQRKEERR AKLQAQFSQM RPAFIPGVGP RMPIFTGGAP GLGQQIFYGQ
460 470 480 490 500
GPPPIIPHQP GFGYQPQLVP GMRPAFFGGP MMQPGQQGPR PGGRRSGDGP
510 520 530 540 550
MRHQHQQPMP YMQPQMMPRG RGYRYPSGGR NMPDGPMPGG MVPVAYDMNV
560 570 580 590 600
MPYSQPMSAG QLATSLANAT PAQQRTLLGE SLYPLVDQIE SEHAAKVTGM
610 620 630 640 650
LLEMDQTEVL HLLESPEALN AKVSEALDVL RNVNQPSSQG SEGNKSGSPS
660
DLLASLSIND HL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 642 | E → D in AAK25927 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002391 Genomic DNA Translation: AAB87097.1 CP002685 Genomic DNA Translation: AEC07446.1 AF360217 mRNA Translation: AAK25927.1 AY050859 mRNA Translation: AAK92796.1 AY079389 mRNA Translation: AAL85120.1 |
PIRi | T00497 |
RefSeqi | NP_179916.1, NM_127899.4 |
Genome annotation databases
EnsemblPlantsi | AT2G23350.1; AT2G23350.1; AT2G23350 |
GeneIDi | 816867 |
Gramenei | AT2G23350.1; AT2G23350.1; AT2G23350 |
KEGGi | ath:AT2G23350 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002391 Genomic DNA Translation: AAB87097.1 CP002685 Genomic DNA Translation: AEC07446.1 AF360217 mRNA Translation: AAK25927.1 AY050859 mRNA Translation: AAK92796.1 AY079389 mRNA Translation: AAL85120.1 |
PIRi | T00497 |
RefSeqi | NP_179916.1, NM_127899.4 |
3D structure databases
SMRi | O22173 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2219, 5 interactors |
IntActi | O22173, 4 interactors |
STRINGi | 3702.AT2G23350.1 |
PTM databases
iPTMneti | O22173 |
MetOSitei | O22173 |
Proteomic databases
PaxDbi | O22173 |
PRIDEi | O22173 |
ProMEXi | O22173 |
ProteomicsDBi | 248630 |
Genome annotation databases
EnsemblPlantsi | AT2G23350.1; AT2G23350.1; AT2G23350 |
GeneIDi | 816867 |
Gramenei | AT2G23350.1; AT2G23350.1; AT2G23350 |
KEGGi | ath:AT2G23350 |
Organism-specific databases
Araporti | AT2G23350 |
TAIRi | locus:2058573, AT2G23350 |
Phylogenomic databases
eggNOGi | KOG0123, Eukaryota |
HOGENOMi | CLU_012062_22_4_1 |
InParanoidi | O22173 |
OMAi | MIRITYS |
OrthoDBi | 1027234at2759 |
PhylomeDBi | O22173 |
Miscellaneous databases
PROi | PR:O22173 |
Gene expression databases
ExpressionAtlasi | O22173, baseline and differential |
Genevisiblei | O22173, AT |
Family and domain databases
Gene3Di | 3.30.70.330, 4 hits |
InterProi | View protein in InterPro IPR012677, Nucleotide-bd_a/b_plait_sf IPR036053, PABP-dom IPR006515, PABP_1234 IPR002004, PABP_HYD IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR003954, RRM_dom_euk |
Pfami | View protein in Pfam PF00658, PABP, 1 hit PF00076, RRM_1, 4 hits |
SMARTi | View protein in SMART SM00517, PolyA, 1 hit SM00360, RRM, 4 hits SM00361, RRM_1, 4 hits |
SUPFAMi | SSF54928, SSF54928, 3 hits SSF63570, SSF63570, 1 hit |
TIGRFAMsi | TIGR01628, PABP-1234, 1 hit |
PROSITEi | View protein in PROSITE PS51309, PABC, 1 hit PS50102, RRM, 4 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PABP4_ARATH | |
Accessioni | O22173Primary (citable) accession number: O22173 Secondary accession number(s): Q9C5J0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 26, 2013 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families