Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable pectinesterase/pectinesterase inhibitor 17

Gene

PME17

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (PMEPCRA), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At5g45990), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At3g06710), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g50310), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At3g31890), Pectinesterase (PMEPCRF), Pectinesterase (At3g10720), Putative pectinesterase 10 (PME10), Putative pectinesterase 11 (PME11), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 14 (PME14), Probable pectinesterase 15 (PME15), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase/pectinesterase inhibitor 18 (PME18), Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Pectinesterase 1 (PME1), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase 29 (PME29), Pectinesterase 2 (PME2), Probable pectinesterase 30 (PME30), Pectinesterase 31 (PME31), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase/pectinesterase inhibitor 3 (PME3), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Pectinesterase 4 (PME4), Probable pectinesterase 50 (PME50), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Putative pectinesterase 52 (PME52), Probable pectinesterase 53 (PME53), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Probable pectinesterase 55 (PME55), Probable pectinesterase 56 (PME56), Putative pectinesterase 57 (PME57), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Pectinesterase QRT1 (QRT1), Putative pectinesterase 63 (PME63), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Probable pectinesterase 66 (PME66), Probable pectinesterase 67 (PME67), Probable pectinesterase 68 (PME68), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Probable pectinesterase 8 (PME8), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At3g30550), Pectinesterase (At3g14310), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g18720), Pectinesterase (At4g15980), Pectinesterase (At3g49220), Pectinesterase (AXX17_At2g22270), Pectinesterase (AXX17_At4g38050), Uncharacterized protein (AXX17_At3g05180), Pectinesterase (AXX17_At5g47900), Pectinesterase, Pectinesterase (AXX17_At5g19650), Pectinesterase (AXX17_At3g43380), Pectinesterase (AXX17_At4g02900), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At3g10570), Pectinesterase (AXX17_At3g56460), Plant invertase/pectin methylesterase inhibitor superfamily (At3g49220), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At1g11900), PME26 (AXX17_At3g14890), Uncharacterized protein (AXX17_At5g27870), Uncharacterized protein (AXX17_At5g64270), Pectinesterase (AXX17_At1g04730), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At5g04430), Pectinesterase, Pectinesterase (F14I3.7), Pectinesterase (AXX17_At2g22280), Pectinesterase, Pectinesterase (AXX17_At5g52320), Pectinesterase (AXX17_At2g33420), Pectinesterase, Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At2g40510), Pectinesterase (At5g20860), Pectinesterase (AXX17_At3g26070), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g38040), Pectinesterase (AXX17_At2g33410), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At3g05190), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At4g02930), Pectinesterase (AXX17_At3g17940), Pectinesterase (AXX17_At2g45340), Plant invertase/pectin methylesterase inhibitor superfamily (At5g09760), Pectinesterase (At5g26810), Pectinesterase (PME44), Pectinesterase (At1g53840)
  2. Pectate lyase (AXX17_At3g06910), Pectate lyase (At3g27400), Pectate lyase (AXX17_At3g26200), Pectate lyase (AXX17_At3g09180), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At3g49720), Pectate lyase (AXX17_At4g25610), Putative pectate lyase 14 (At4g13210), Probable pectate lyase 19 (At5g15110), Probable pectate lyase 3 (AT59), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 6 (At2g02720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 12 (At3g53190), Putative pectate lyase 17 (At4g22090), Probable pectate lyase 10 (At3g24670), Probable pectate lyase 13 (PMR6), Probable pectate lyase 1 (At1g04680), Probable pectate lyase 20 (At5g48900), Probable pectate lyase 4 (At1g30350), Probable pectate lyase 16 (At4g22080), Probable pectate lyase 18 (At4g24780), Putative pectate lyase 21 (At5g55720), Probable pectate lyase 22 (At5g63180), Putative pectate lyase 2 (At1g11920), Putative pectate lyase 11 (At3g27400), Probable pectate lyase 15 (At4g13710), Pectate lyase (At5g09280), Pectate lyase (At3g55140), Pectate lyase, Pectate lyase (At1g14420), Pectate lyase (F11F8_12), Pectate lyase (At5g04310), Pectate lyase (AXX17_At3g47590), Pectate lyase (At3g09540), Pectate lyase (AXX17_At5g54880), Pectate lyase, Pectate lyase (AXX17_At5g08800), Pectate lyase (AXX17_At1g04020), Pectate lyase (AXX17_At1g15010), Pectate lyase (AXX17_At1g12300), Pectate lyase (At3g01270), Pectate lyase (AXX17_At2g01680), Pectate lyase (At3g55140), Pectate lyase (AXX17_At3g00310), Pectate lyase (AXX17_At5g47570), Pectate lyase (AXX17_At5g03700), Pectate lyase (AXX17_At1g30780), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At3g29900), Pectate lyase (At3g07010), Pectate lyase (AXX17_At5g14600), Pectate lyase (AXX17_At1g61710), Pectate lyase (AXX17_At5g62760), Pectate lyase (At3g07010), Pectate lyase (At3g01270), Pectate lyase, Pectate lyase (AXX17_At4g25600), Pectate lyase (At5g04310), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At4g15660), Pectate lyase (At3g53190), Pectate lyase, Pectate lyase (At4g13710), Pectate lyase (At4g13210), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g26720), Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei277Substrate; for pectinesterase activityBy similarity1
Binding sitei307Substrate; for pectinesterase activityBy similarity1
Sitei329Transition state stabilizerBy similarity1
Active sitei330Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei351Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei418Substrate; for pectinesterase activityBy similarity1
Binding sitei420Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

  • aspartyl esterase activity Source: UniProtKB-KW
  • pectinesterase activity Source: GO_Central
  • pectinesterase inhibitor activity Source: GO_Central

GO - Biological processi

  • cell wall modification Source: InterPro
  • defense response to Gram-negative bacterium Source: TAIR
  • pectin catabolic process Source: UniProtKB-UniPathway
  • response to bacterium Source: TAIR
  • response to fungus Source: TAIR

Keywordsi

Molecular functionAspartyl esterase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G45220-MONOMER
UniPathwayi
UPA00545;UER00823

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 17
Including the following 2 domains:
Pectinesterase inhibitor 17
Alternative name(s):
Pectin methylesterase inhibitor 17
Pectinesterase 17 (EC:3.1.1.11)
Short name:
PE 17
Alternative name(s):
Pectin methylesterase 17
Short name:
AtPME17
Gene namesi
Name:PME17
Synonyms:ARATH17
Ordered Locus Names:At2g45220
ORF Names:F4L23.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G45220
TAIRilocus:2050941 AT2G45220

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000037167424 – 511Probable pectinesterase/pectinesterase inhibitor 17Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi160N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi344 ↔ 364By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO22149
PRIDEiO22149

Expressioni

Tissue specificityi

Expressed in siliques.2 Publications

Developmental stagei

Expressed during late developmental phases of siliques.1 Publication

Gene expression databases

ExpressionAtlasiO22149 baseline and differential
GenevisibleiO22149 AT

Interactioni

Protein-protein interaction databases

BioGridi4466, 2 interactors
IntActiO22149, 2 interactors
STRINGi3702.AT2G45220.1

Structurei

3D structure databases

ProteinModelPortaliO22149
SMRiO22149
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 171Pectinesterase inhibitor 17Add BLAST148
Regioni237 – 414Pectinesterase 17Add BLAST178

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IF38 Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiO22149
KOiK01051
OMAiFLTHNSN
OrthoDBiEOG093607RJ
PhylomeDBiO22149

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR006633 Carb-bd_sugar_hydrolysis-dom
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00722 CASH, 1 hit
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22149-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MMAFRAYIIN FVILCILVAS TVSGYNQKDV KAWCSQTPNP KPCEYFLTHN
60 70 80 90 100
SNNEPIKSES EFLKISMKLV LDRAILAKTH AFTLGPKCRD TREKAAWEDC
110 120 130 140 150
IKLYDLTVSK INETMDPNVK CSKLDAQTWL STALTNLDTC RAGFLELGVT
160 170 180 190 200
DIVLPLMSNN VSNLLCNTLA INKVPFNYTP PEKDGFPSWV KPGDRKLLQS
210 220 230 240 250
STPKDNAVVA KDGSGNFKTI KEAIDAASGS GRFVIYVKQG VYSENLEIRK
260 270 280 290 300
KNVMLRGDGI GKTIITGSKS VGGGTTTFNS ATVAAVGDGF IARGITFRNT
310 320 330 340 350
AGASNEQAVA LRSGSDLSVF YQCSFEAYQD TLYVHSNRQF YRDCDVYGTV
360 370 380 390 400
DFIFGNAAAV LQNCNIFARR PRSKTNTITA QGRSDPNQNT GIIIHNSRVT
410 420 430 440 450
AASDLRPVLG STKTYLGRPW RQYSRTVFMK TSLDSLIDPR GWLEWDGNFA
460 470 480 490 500
LKTLFYAEFQ NTGPGASTSG RVTWPGFRVL GSASEASKFT VGTFLAGGSW
510
IPSSVPFTSG L
Length:511
Mass (Da):55,977
Last modified:June 1, 2002 - v2
Checksum:i96487096A87F9375
GO

Sequence cautioni

The sequence BAD93862 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002387 Genomic DNA Translation: AAB82640.2
CP002685 Genomic DNA Translation: AEC10527.1
AF361829 mRNA Translation: AAK32841.1
AK220726 mRNA Translation: BAD93862.1 Different initiation.
PIRiH84887
RefSeqiNP_566038.1, NM_130085.4
UniGeneiAt.27946

Genome annotation databases

EnsemblPlantsiAT2G45220.1; AT2G45220.1; AT2G45220
GeneIDi819130
GrameneiAT2G45220.1; AT2G45220.1; AT2G45220
KEGGiath:AT2G45220

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002387 Genomic DNA Translation: AAB82640.2
CP002685 Genomic DNA Translation: AEC10527.1
AF361829 mRNA Translation: AAK32841.1
AK220726 mRNA Translation: BAD93862.1 Different initiation.
PIRiH84887
RefSeqiNP_566038.1, NM_130085.4
UniGeneiAt.27946

3D structure databases

ProteinModelPortaliO22149
SMRiO22149
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4466, 2 interactors
IntActiO22149, 2 interactors
STRINGi3702.AT2G45220.1

Proteomic databases

PaxDbiO22149
PRIDEiO22149

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G45220.1; AT2G45220.1; AT2G45220
GeneIDi819130
GrameneiAT2G45220.1; AT2G45220.1; AT2G45220
KEGGiath:AT2G45220

Organism-specific databases

AraportiAT2G45220
TAIRilocus:2050941 AT2G45220

Phylogenomic databases

eggNOGiENOG410IF38 Eukaryota
COG4677 LUCA
HOGENOMiHOG000217409
InParanoidiO22149
KOiK01051
OMAiFLTHNSN
OrthoDBiEOG093607RJ
PhylomeDBiO22149

Enzyme and pathway databases

UniPathwayi
UPA00545;UER00823

BioCyciARA:AT2G45220-MONOMER

Miscellaneous databases

PROiPR:O22149

Gene expression databases

ExpressionAtlasiO22149 baseline and differential
GenevisibleiO22149 AT

Family and domain databases

Gene3Di1.20.140.40, 1 hit
2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR006633 Carb-bd_sugar_hydrolysis-dom
IPR035513 Invertase/methylesterase_inhib
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
IPR033131 Pectinesterase_Asp_AS
IPR000070 Pectinesterase_cat
IPR006501 Pectinesterase_inhib_dom
PfamiView protein in Pfam
PF01095 Pectinesterase, 1 hit
PF04043 PMEI, 1 hit
SMARTiView protein in SMART
SM00722 CASH, 1 hit
SM00856 PMEI, 1 hit
SUPFAMiSSF101148 SSF101148, 1 hit
SSF51126 SSF51126, 1 hit
TIGRFAMsiTIGR01614 PME_inhib, 1 hit
PROSITEiView protein in PROSITE
PS00503 PECTINESTERASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPME17_ARATH
AccessioniPrimary (citable) accession number: O22149
Secondary accession number(s): Q570I2, Q9ASU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 1, 2002
Last modified: November 7, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again