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UniProtKB - O22133 (BEN1_ARATH)
Protein
Protein BRI1-5 ENHANCED 1
Gene
BEN1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Element of the brassinosteroid metabolic pathway that regulates typhasterol, castasterone and brassinolide levels (PubMed:17521414).
Involved in the control of organ elongation (PubMed:17521414, PubMed:23893742).
2 PublicationsMiscellaneous
The ben1-1D (bri1-5 enhanced 1-1dominant) activation-tagging mutant suppresses the bri1-5 weak mutant allele of the brassinosteroid receptor gene BRI1.1 Publication
: brassinosteroid biosynthesis Pathwayi
This protein is involved in the pathway brassinosteroid biosynthesis, which is part of Plant hormone biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway brassinosteroid biosynthesis and in Plant hormone biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 69 | NADPBy similarity | 1 | |
Binding sitei | 202 | NADPBy similarity | 1 | |
Active sitei | 206 | Proton donorBy similarity | 1 | |
Binding sitei | 206 | NADPBy similarity | 1 | |
Binding sitei | 232 | NADP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 244 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 44 – 49 | NADPBy similarity | 6 | |
Nucleotide bindingi | 98 – 99 | NADPBy similarity | 2 |
GO - Molecular functioni
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: GO_Central
GO - Biological processi
- brassinosteroid biosynthetic process Source: UniProtKB-UniPathway
- brassinosteroid metabolic process Source: TAIR
- regulation of brassinosteroid biosynthetic process Source: TAIR
- response to absence of light Source: UniProtKB
Keywordsi
Molecular function | Developmental protein, Oxidoreductase |
Biological process | Brassinosteroid biosynthesis, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
Ligand | NADP |
Enzyme and pathway databases
BioCyci | ARA:AT2G45400-MONOMER |
UniPathwayi | UPA00381 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:BEN11 Publication Ordered Locus Names:At2g45400Imported ORF Names:F4L23.9Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress)Imported |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G45400 |
TAIRi | locus:2050882, AT2G45400 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Other locations
- cytoplasm Source: TAIR
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Disruption phenotypei
Abnormal organs elongation leading to long inflorescences, leaves and petioles, especially in the light.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000434413 | 1 – 364 | Protein BRI1-5 ENHANCED 1Add BLAST | 364 |
Proteomic databases
PaxDbi | O22133 |
ProteomicsDBi | 240778 |
Expressioni
Tissue specificityi
Mainly present in cell elongating-containing tissues. Strongly expressed in roots and flowers, also observed in petioles, stems, leaves and siliques.1 Publication
Developmental stagei
First observed after seed germination, mainly in the root cap, and in elongation and maturation zones, and, to a lower extent, in the apical meristem zone. Later present in roots, with higher levels in light conditions than in darkness. Weak levels in young flowers. Progressive accumulation in developing siliques, at both ends. In rosette leaves, mainly localized in vascular tissues and hydathodes.1 Publication
Inductioni
Down-regulated in the dark.1 Publication
Gene expression databases
ExpressionAtlasi | O22133, baseline and differential |
Genevisiblei | O22133, AT |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
IntActi | O22133, 1 interactor |
STRINGi | 3702.AT2G45400.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 |
Sequence similaritiesi
Belongs to the NAD(P)-dependent epimerase/dehydratase family.Curated
Phylogenomic databases
eggNOGi | KOG1502, Eukaryota |
HOGENOMi | CLU_007383_9_0_1 |
InParanoidi | O22133 |
OrthoDBi | 992332at2759 |
PhylomeDBi | O22133 |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
O22133-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVREEQEEDD NNNNNNGGGE RKLLVADETV PSLLDETGLV CVTGGSGFVA
60 70 80 90 100
SWLIMRLLQR GYSVRATVRT NSEGNKKDIS YLTELPFASE RLQIFTADLN
110 120 130 140 150
EPESFKPAIE GCKAVFHVAH PMDPNSNETE ETVTKRTVQG LMGILKSCLD
160 170 180 190 200
AKTVKRFFYT SSAVTVFYSG GNGGGGGEVD ESVWSDVEVF RNQKEKRVSS
210 220 230 240 250
SYVVSKMAAE TAALEFGGKN GLEVVTLVIP LVVGPFISSS LPSSVFISLA
260 270 280 290 300
MLFGNYKEKY LFDTYNMVHI DDVARAMIFL LEKPVAKGRY ICSSVEMKID
310 320 330 340 350
EVFEFLSTKF PQFQLPSIDL NKYKVEKRMG LSSKKLKSAG FEFKYGAEEI
360
FSGAIRSCQA RGFL
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AYD5 | A0A1P8AYD5_ARATH | NAD(P)-binding Rossmann-fold superf... | BEN1 BRI1-5 ENHANCED 1, At2g45400, F4L23.9 | 324 | Annotation score: | ||
A0A1P8AYJ2 | A0A1P8AYJ2_ARATH | NAD(P)-binding Rossmann-fold superf... | BEN1 BRI1-5 ENHANCED 1, At2g45400, F4L23.9 | 255 | Annotation score: | ||
A0A1P8AYJ1 | A0A1P8AYJ1_ARATH | NAD(P)-binding Rossmann-fold superf... | BEN1 BRI1-5 ENHANCED 1, At2g45400, F4L23.9 | 307 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002387 Genomic DNA Translation: AAB82624.1 CP002685 Genomic DNA Translation: AEC10546.1 |
PIRi | A84890 |
RefSeqi | NP_182064.1, NM_130102.5 |
Genome annotation databases
EnsemblPlantsi | AT2G45400.1; AT2G45400.1; AT2G45400 |
GeneIDi | 819146 |
Gramenei | AT2G45400.1; AT2G45400.1; AT2G45400 |
KEGGi | ath:AT2G45400 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002387 Genomic DNA Translation: AAB82624.1 CP002685 Genomic DNA Translation: AEC10546.1 |
PIRi | A84890 |
RefSeqi | NP_182064.1, NM_130102.5 |
3D structure databases
SMRi | O22133 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O22133, 1 interactor |
STRINGi | 3702.AT2G45400.1 |
Proteomic databases
PaxDbi | O22133 |
ProteomicsDBi | 240778 |
Genome annotation databases
EnsemblPlantsi | AT2G45400.1; AT2G45400.1; AT2G45400 |
GeneIDi | 819146 |
Gramenei | AT2G45400.1; AT2G45400.1; AT2G45400 |
KEGGi | ath:AT2G45400 |
Organism-specific databases
Araporti | AT2G45400 |
TAIRi | locus:2050882, AT2G45400 |
Phylogenomic databases
eggNOGi | KOG1502, Eukaryota |
HOGENOMi | CLU_007383_9_0_1 |
InParanoidi | O22133 |
OrthoDBi | 992332at2759 |
PhylomeDBi | O22133 |
Enzyme and pathway databases
UniPathwayi | UPA00381 |
BioCyci | ARA:AT2G45400-MONOMER |
Miscellaneous databases
PROi | PR:O22133 |
Gene expression databases
ExpressionAtlasi | O22133, baseline and differential |
Genevisiblei | O22133, AT |
Family and domain databases
InterProi | View protein in InterPro IPR001509, Epimerase_deHydtase IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF01370, Epimerase, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BEN1_ARATH | |
Accessioni | O22133Primary (citable) accession number: O22133 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 14, 2015 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families