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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

NAD11

Organism
Reclinomonas americana
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi45Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi48Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi62Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi94Iron-sulfur 2 (4Fe-4S); via tele nitrogenPROSITE-ProRule annotation1
Metal bindingi98Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi101Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi107Iron-sulfur 2 (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi146Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi149Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi196Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Translocase
Biological processElectron transport, Respiratory chain, Transport
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.99.3, EC:7.1.1.2)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
NADH dehydrogenase subunit 11
Gene namesi
Name:NAD11
Encoded oniMitochondrion
OrganismiReclinomonas americana
Taxonomic identifieri48483 [NCBI]
Taxonomic lineageiEukaryotaJakobidaHistionidaeReclinomonas

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185371 – 691NADH-ubiquinone oxidoreductase 75 kDa subunitAdd BLAST691

Interactioni

Subunit structurei

Complex I is composed of about 30 different subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliO21241
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 782Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST78
Domaini78 – 1174Fe-4S His(Cys)3-ligated-typePROSITE-ProRule annotationAdd BLAST40
Domaini215 – 2714Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NuoG_C
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
PF09326 NADH_dhqG_C, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit

Sequencei

Sequence statusi: Complete.

O21241-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVNVFVDGLS VEVKKGATIL QACAQVGIEI PRFCYHERLS IAGNCRMCLV
60 70 80 90 100
EVEKSPKPVA SCAMPVMDNM KIFTNTPLVK KAREGVLEFL LVNHPLDCPI
110 120 130 140 150
CDQGGECDLQ DLTMVYGSDR GRFHEYKRGV EDKNIGPLVK TVMTRCIHCT
160 170 180 190 200
RCVRFATEVA GVPDLGTVGR GRDTEISTYI QKVFNSELSG NVIDLCPVGA
210 220 230 240 250
LTSKPYAFTA RSWELQSTES IDVSDAIGSN IRIDVRGSEI MRILPRLNED
260 270 280 290 300
VNEEWISDKA RFCYDGLKRQ RLNNPIIKEN GQYKTVTWEK AFNFILKNLQ
310 320 330 340 350
EIQNSNRIVG VVGNLMDVES ILLFKELLNK LGSSKIYLES STPILQLNDD
360 370 380 390 400
EKEDQILNNA DFRNNYISNT PLAKIEESDL CLLIGTNIRL EAPLLNTRIR
410 420 430 440 450
KRYLQGNYSV YSVGPTNNLT YNTENLGNDI STLLEISEGR HPFCKKLMKS
460 470 480 490 500
KKPLIIIGTH VLQRTDGTSI IELVKTLFKY TQIKTSNWNG FNILHTSASS
510 520 530 540 550
VGALDLGIGS TKRYSEKISN SKIEKHFIYL LGADEIRIEN SKEHFIVYQG
560 570 580 590 600
HHGDYGANIA DVILPGSAYT EKTATYVNVE GRVQNTKSAF YAPGNAREDW
610 620 630 640 650
KIIRALSEVL NKKLPYDSFE DIHTRFMSIA PHLLKVNAIE KNKIVIENSL
660 670 680 690
PFKGLIKNIG FKPLFNNFYL TNAICRSSQT MAKCSSIYKL N
Length:691
Mass (Da):77,718
Last modified:January 1, 1998 - v1
Checksum:i1FB45AF558F469E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA Translation: AAD11868.1
PIRiS78135
RefSeqiNP_044753.1, NC_001823.1

Genome annotation databases

GeneIDi801135

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA Translation: AAD11868.1
PIRiS78135
RefSeqiNP_044753.1, NC_001823.1

3D structure databases

ProteinModelPortaliO21241
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi801135

Family and domain databases

CDDicd00207 fer2, 1 hit
InterProiView protein in InterPro
IPR036010 2Fe-2S_ferredoxin-like_sf
IPR001041 2Fe-2S_ferredoxin-type
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR000283 NADH_UbQ_OxRdtase_75kDa_su_CS
IPR010228 NADH_UbQ_OxRdtase_Gsu
IPR019574 NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd
IPR015405 NuoG_C
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF10588 NADH-G_4Fe-4S_3, 1 hit
PF09326 NADH_dhqG_C, 1 hit
SMARTiView protein in SMART
SM00929 NADH-G_4Fe-4S_3, 1 hit
SUPFAMiSSF54292 SSF54292, 1 hit
TIGRFAMsiTIGR01973 NuoG, 1 hit
PROSITEiView protein in PROSITE
PS51085 2FE2S_FER_2, 1 hit
PS51839 4FE4S_HC3, 1 hit
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00641 COMPLEX1_75K_1, 1 hit
PS00642 COMPLEX1_75K_2, 1 hit
PS00643 COMPLEX1_75K_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS1_RECAM
AccessioniPrimary (citable) accession number: O21241
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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