UniProtKB - O20965 (CYB_TRAIM)
Protein
Cytochrome b
Gene
MT-CYB
Organism
Tragelaphus imberbis (Lesser kudu)
Status
Functioni
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.By similarity
Miscellaneous
Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.By similarity
Caution
The full-length protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.By similarity
Cofactori
hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 83 | Iron 1 (heme b562 axial ligand)By similarity | 1 | |
Metal bindingi | 97 | Iron 2 (heme b566 axial ligand)By similarity | 1 | |
Metal bindingi | 182 | Iron 1 (heme b562 axial ligand)By similarity | 1 | |
Metal bindingi | 196 | Iron 2 (heme b566 axial ligand)By similarity | 1 | |
Binding sitei | 201 | UbiquinoneBy similarity | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquinol-cytochrome-c reductase activity Source: InterPro
GO - Biological processi
- respiratory electron transport chain Source: InterPro
Keywordsi
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | Heme, Iron, Metal-binding, Ubiquinone |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome bAlternative name(s): Complex III subunit 3 Complex III subunit III Cytochrome b-c1 complex subunit 3 Ubiquinol-cytochrome-c reductase complex cytochrome b subunit |
Gene namesi | Name:MT-CYB Synonyms:COB, CYTB, MTCYB |
Encoded oni | Mitochondrion |
Organismi | Tragelaphus imberbis (Lesser kudu) |
Taxonomic identifieri | 9947 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Tragelaphus |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- respiratory chain complex III Source: InterPro
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 33 – 53 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 77 – 98 | HelicalBy similarityAdd BLAST | 22 | |
Transmembranei | 113 – 133 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 178 – 198 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 226 – 246 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 288 – 308 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 320 – 340 | HelicalBy similarityAdd BLAST | 21 | |
Transmembranei | 347 – 367 | HelicalBy similarityAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000061676 | 1 – 379 | Cytochrome bAdd BLAST | 379 |
Interactioni
Subunit structurei
The cytochrome bc1 complex contains 11 subunits: 3 respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). This cytochrome bc1 complex then forms a dimer.
By similarityFamily & Domainsi
Sequence similaritiesi
Belongs to the cytochrome b family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixFamily and domain databases
CDDi | cd00290, cytochrome_b_C, 1 hit cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit |
InterProi | View protein in InterPro IPR005798, Cyt_b/b6_C IPR036150, Cyt_b/b6_C_sf IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR030689, Cytochrome_b IPR016174, Di-haem_cyt_TM |
Pfami | View protein in Pfam PF00032, Cytochrom_B_C, 1 hit PF00033, Cytochrome_B, 1 hit |
PIRSFi | PIRSF038885, COB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit SSF81648, SSF81648, 1 hit |
PROSITEi | View protein in PROSITE PS51003, CYTB_CTER, 1 hit PS51002, CYTB_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
O20965-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MINIRKSHPL MKIVNNAFID LPTPPNISSW WNFGSLLGIC LVLQILTGLF
60 70 80 90 100
LAMHYTSDTM TAFSSVTHIC RDVNYGWIIR YMHANGASMF FICLYMHVGR
110 120 130 140 150
GLYYGSYTFL ETWNIGVILL FTVMATAFMG YVLPWGQMSF WGATVITNLL
160 170 180 190 200
SAIPYIGTNL VEWIWGGFSV DKATLTRFFA FHFILPFIIA ALALVHLLFL
210 220 230 240 250
HETGSNNPTG ISSDTDKIPF HPYYTIKDIL GALLLILALM LLVLFAPDLL
260 270 280 290 300
GDPDNYTPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALILTILM
310 320 330 340 350
PILMPLLHAS KQRSMMFRPL SQCLFWILVA DLLTLTWIGG QPVEHPYIII
360 370
GQLASIMYFL LILVLMPMAG SIENNLLKW
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF022064 Genomic DNA Translation: AAD13498.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF022064 Genomic DNA Translation: AAD13498.1 |
3D structure databases
SMRi | O20965 |
ModBasei | Search... |
Family and domain databases
CDDi | cd00290, cytochrome_b_C, 1 hit cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit |
InterProi | View protein in InterPro IPR005798, Cyt_b/b6_C IPR036150, Cyt_b/b6_C_sf IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR030689, Cytochrome_b IPR016174, Di-haem_cyt_TM |
Pfami | View protein in Pfam PF00032, Cytochrom_B_C, 1 hit PF00033, Cytochrome_B, 1 hit |
PIRSFi | PIRSF038885, COB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit SSF81648, SSF81648, 1 hit |
PROSITEi | View protein in PROSITE PS51003, CYTB_CTER, 1 hit PS51002, CYTB_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYB_TRAIM | |
Accessioni | O20965Primary (citable) accession number: O20965 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | August 1, 1999 | |
Last modified: | December 2, 2020 | |
This is version 88 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |