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Entry version 91 (02 Jun 2021)
Sequence version 1 (01 Jan 1998)
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Protein

Transketolase, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the reversible transfer of a two-carbon ketol group from fructose-6-phosphate or sedoheptulose-7-phosphate to glyceraldehyde-3-phosphate to yield xylulose-5-phosphate and erythrose-4-phosphate or ribose-5-phosphate, respectively.

1 Publication1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Protein has several cofactor binding sites:

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=67 µM for xylulose 5-phosphate1 Publication
  2. KM=330 µM for ribose 5-phosphate1 Publication

    pH dependencei

    Optimum pH is 8.0.1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: Calvin cycle

    This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei103SubstrateBy similarity1
    <p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei103Important for catalytic activityBy similarity1
    Binding sitei143Thiamine pyrophosphateBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi233MagnesiumBy similarity1
    Binding sitei234Thiamine pyrophosphate; via amide nitrogenBy similarity1
    Metal bindingi263MagnesiumBy similarity1
    Binding sitei263Thiamine pyrophosphateBy similarity1
    Metal bindingi265Magnesium; via carbonyl oxygenBy similarity1
    Binding sitei340SubstrateBy similarity1
    Binding sitei340Thiamine pyrophosphateBy similarity1
    Sitei340Important for catalytic activityBy similarity1
    Binding sitei434SubstrateBy similarity1
    Binding sitei461SubstrateBy similarity1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei488Proton donorBy similarity1
    Binding sitei488Thiamine pyrophosphateBy similarity1
    Binding sitei515Thiamine pyrophosphateBy similarity1
    Binding sitei539SubstrateBy similarity1
    Binding sitei547SubstrateBy similarity1
    Binding sitei598SubstrateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi192 – 194Thiamine pyrophosphateBy similarity3

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionTransferase
    LigandCalcium, Magnesium, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    O20250

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00116

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Transketolase, chloroplastic (EC:2.2.1.1)
    Short name:
    TK
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSpinacia oleracea (Spinach)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri3562 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliopsidaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a transit peptide.<p><a href='/help/transit' target='_top'>More...</a></p>Transit peptidei1 – 66Chloroplast1 PublicationAdd BLAST66
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_000003575267 – 741Transketolase, chloroplasticAdd BLAST675

    Proteomic databases

    PRoteomics IDEntifications database

    More...
    PRIDEi
    O20250

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    1 Publication

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    O20250

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 33DisorderedSequence analysisAdd BLAST33

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Belongs to the transketolase family.1 Publication

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd02012, TPP_TK, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.40.50.920, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR029061, THDP-binding
    IPR009014, Transketo_C/PFOR_II
    IPR005475, Transketolase-like_Pyr-bd
    IPR005478, Transketolase_bac-like
    IPR033248, Transketolase_C
    IPR033247, Transketolase_fam
    IPR005474, Transketolase_N

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR43522, PTHR43522, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF02779, Transket_pyr, 1 hit
    PF02780, Transketolase_C, 1 hit
    PF00456, Transketolase_N, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00861, Transket_pyr, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF52518, SSF52518, 2 hits
    SSF52922, SSF52922, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00232, tktlase_bact, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00801, TRANSKETOLASE_1, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

    O20250-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MAASSSLSTL SHHQTLLSHP KTHLPTTPAS SLLVPTTSSK VNGVLLKSTS
    60 70 80 90 100
    SSRRLRVGSA SAVVRAAAVE ALESTDIDQL VEKSVNTIRF LAIDAVEKAN
    110 120 130 140 150
    SGHPGLPMGC APMGHILYDE IMRYNPKNPY WFNRDRFVLS AGHGCMLQYA
    160 170 180 190 200
    LLHLAGYDSV LEEDLKTFRQ WGSRIPGHPE NFETPGVEVT TGPLGQGIAN
    210 220 230 240 250
    AVGLALAEKH LAARFNKPDA EIVDHYTYVI LGDGCQMEGI AQEACSLAGH
    260 270 280 290 300
    WGLGKLIAFY DDNHISIDGD TAIAFTESVD LRFEALGWHV IWVKNGNTGY
    310 320 330 340 350
    DEIRAAIKEA KTVTDKPTLI KVTTTIGFGS PNKSNSYSVH GSALGSKEVE
    360 370 380 390 400
    ATRQNLGWPY EPFHVPEEVK KHWSRHTPEG ASLEAEWNTK FAEYEKKYPE
    410 420 430 440 450
    DATEFKSITT GEFPAGWEKA LPTYTPETPG DATRNLSQQC LNALAKVIPG
    460 470 480 490 500
    LLGGSADLAS SNMTLLKMFG DFRRTHRKKE TFRFGVREHG MGAICNGICL
    510 520 530 540 550
    HSPGFVPYCA TFFVFTDYMR GAMRISALSE AGVIYVMTHD SIGLGEDGPT
    560 570 580 590 600
    HQPIEALSKF PAMPNILMLR PADGNETAGS YKVAVENRKT PSILALSRKK
    610 620 630 640 650
    LPNLPGTSIE GVEKGGYTIT DNSSGNKPDV ILIGTGSELE IAAKAGDELR
    660 670 680 690 700
    KEGKAVRVVS FVSWELFEKQ SDEYKESVLP SDVTARVSIE AGSTFGWHKI
    710 720 730 740
    VGSKGKAIGI DKFGASAPAG KIYQEYGITV EAVVEAAKSV C
    Length:741
    Mass (Da):80,281
    Last modified:January 1, 1998 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i28871ED8568EAC8B
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti77I → D AA sequence (PubMed:9523694).Curated1
    Sequence conflicti83K → G AA sequence (PubMed:9523694).Curated1

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    L76554 mRNA Translation: AAD10219.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    T09015

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L76554 mRNA Translation: AAD10219.1
    PIRiT09015

    3D structure databases

    SMRiO20250
    ModBaseiSearch...

    Proteomic databases

    PRIDEiO20250

    Enzyme and pathway databases

    UniPathwayiUPA00116
    SABIO-RKiO20250

    Family and domain databases

    CDDicd02012, TPP_TK, 1 hit
    Gene3Di3.40.50.920, 1 hit
    InterProiView protein in InterPro
    IPR029061, THDP-binding
    IPR009014, Transketo_C/PFOR_II
    IPR005475, Transketolase-like_Pyr-bd
    IPR005478, Transketolase_bac-like
    IPR033248, Transketolase_C
    IPR033247, Transketolase_fam
    IPR005474, Transketolase_N
    PANTHERiPTHR43522, PTHR43522, 1 hit
    PfamiView protein in Pfam
    PF02779, Transket_pyr, 1 hit
    PF02780, Transketolase_C, 1 hit
    PF00456, Transketolase_N, 1 hit
    SMARTiView protein in SMART
    SM00861, Transket_pyr, 1 hit
    SUPFAMiSSF52518, SSF52518, 2 hits
    SSF52922, SSF52922, 1 hit
    TIGRFAMsiTIGR00232, tktlase_bact, 1 hit
    PROSITEiView protein in PROSITE
    PS00801, TRANSKETOLASE_1, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTKTC_SPIOL
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O20250
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: January 1, 1998
    Last modified: June 2, 2021
    This is version 91 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
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