UniProtKB - O19132 (NOS1_RABIT)
Nitric oxide synthase, brain
NOS1
Functioni
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR (By similarity).
By similarityCatalytic activityi
- EC:1.14.13.39
Cofactori
Protein has several cofactor binding sites:- hemeBy similarity
- FADBy similarityNote: Binds 1 FAD.By similarity
- FMNBy similarityNote: Binds 1 FMN.By similarity
- (6R)-L-erythro-5,6,7,8-tetrahydrobiopterinBy similarityNote: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.By similarity
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 421 | Iron (heme axial ligand)By similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 887 – 918 | FMNPROSITE-ProRule annotationAdd BLAST | 32 | |
Nucleotide bindingi | 1033 – 1044 | FADBy similarityAdd BLAST | 12 | |
Nucleotide bindingi | 1176 – 1186 | FADBy similarityAdd BLAST | 11 | |
Nucleotide bindingi | 1251 – 1269 | NADPBy similarityAdd BLAST | 19 | |
Nucleotide bindingi | 1349 – 1364 | NADPBy similarityAdd BLAST | 16 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- flavin adenine dinucleotide binding Source: InterPro
- FMN binding Source: InterPro
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- NADP binding Source: InterPro
- nitric-oxide synthase activity Source: UniProtKB-EC
GO - Biological processi
- nitric oxide biosynthetic process Source: InterPro
Keywordsi
Molecular function | Calmodulin-binding, Oxidoreductase |
Ligand | FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP |
Names & Taxonomyi
Protein namesi | Recommended name: Nitric oxide synthase, brain (EC:1.14.13.39)Alternative name(s): Constitutive NOS NC-NOS NOS type I Neuronal NOS Short name: N-NOS Short name: nNOS Peptidyl-cysteine S-nitrosylase NOS1 bNOS |
Gene namesi | Name:NOS1 |
Organismi | Oryctolagus cuniculus (Rabbit) |
Taxonomic identifieri | 9986 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- sarcolemma By similarity; Peripheral membrane protein By similarity
Other locations
- dendritic spine By similarity
Note: In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).By similarity
Plasma Membrane
- sarcolemma Source: UniProtKB-SubCell
Other locations
- dendritic spine Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Cell projection, Membrane, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000170923 | 1 – 1435 | Nitric oxide synthase, brainAdd BLAST | 1435 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 853 | PhosphoserineBy similarity | 1 | |
Modified residuei | 863 | PhosphoserineBy similarity | 1 | |
Modified residuei | 864 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
PRIDEi | O19132 |
Interactioni
Subunit structurei
Homodimer.
Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON.
Forms a ternary complex with CAPON and RASD1.
Forms a ternary complex with CAPON and SYN1.
Interacts with ZDHHC23.
Interacts with NOSIP; which may impair its synaptic location (By similarity).
Interacts with HTR4.
Interacts with VAC14 (By similarity).
Interacts with SLC6A4 (By similarity).
Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains) (By similarity).
By similarityGO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000020745 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 99 | PDZPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 761 – 941 | Flavodoxin-likePROSITE-ProRule annotationAdd BLAST | 181 | |
Domaini | 996 – 1243 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 248 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 206 | Interaction with NOSIPBy similarityAdd BLAST | 206 | |
Regioni | 110 – 201 | DisorderedSequence analysisAdd BLAST | 92 | |
Regioni | 164 – 246 | PIN (nNOS-inhibiting protein) bindingBy similarityAdd BLAST | 83 | |
Regioni | 277 – 304 | DisorderedSequence analysisAdd BLAST | 28 | |
Regioni | 731 – 751 | Calmodulin-bindingSequence analysisAdd BLAST | 21 | |
Regioni | 756 – 775 | Tetrahydrobiopterin-bindingBy similarityAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 161 – 191 | Polar residuesSequence analysisAdd BLAST | 31 | |
Compositional biasi | 282 – 300 | Polar residuesSequence analysisAdd BLAST | 19 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
InParanoidi | O19132 |
OrthoDBi | 90349at2759 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 2.30.42.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit 3.90.1230.10, 1 hit 3.90.340.10, 1 hit 3.90.440.10, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR044943, NOS_dom_1 IPR044940, NOS_dom_2 IPR044944, NOS_dom_3 IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR001478, PDZ IPR036034, PDZ_sf IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit PF00595, PDZ, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit PS50106, PDZ, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MEEHVFGVQQ IQPNVISVRL FKRKVGGLGF LVKERVSKPP VIISDLIRGG
60 70 80 90 100
AAEQSGLIQA GDIILAVNGR PLVDLSYDSA LEVLRGVASE THVVLILRGP
110 120 130 140 150
EGFTTNLETT FTGDGTPKTI RVTQPLGAPT KAVDLSHQPP SAGKEQPRPV
160 170 180 190 200
DGAAGPGSWP QPTQGHGQEA GSPSRANGLA PRTSSQDPAK KSGWAGLQGS
210 220 230 240 250
GDKNELLKEI EPVLTLLAGG SKAVDGGGPA KAETRDTGVQ VDRDFDAKSH
260 270 280 290 300
KPLPLGVEND RVFSDLWGKG SAPVVLNNPY SEKEQPPASG KQSPTKNGSP
310 320 330 340 350
SKCPRFLKVK NWETDVVLTD TLHLKSTLET GCTEHICMGS IMFPSQHTRR
360 370 380 390 400
PEDIRTKEQL FPLAKEFIDQ YYSSIKRFGS KAHMERLEEV NKEIESTSTY
410 420 430 440 450
QLKDTELIYG AKHAWRNASR CVGRIQWSKL QVFDARDCTT AHGMFNYICN
460 470 480 490 500
HIKYATNKGN LRSAITIFPQ RTDGKHDFRV WNSQLIRYAG YKQPDGSTLG
510 520 530 540 550
DPANVQFTEI CIQQGWKPPR SRFDVLPLLL QANGNDPELF QIPPELVLEV
560 570 580 590 600
PIRHPKFEWF KDLGLKWYGL PAVSNMLLEI GGLEFSACPF SGWYMGTEIG
610 620 630 640 650
VRDYCDNSRY NILEEVAKKM NLDMRKTSSL WKDQALVEIN IAVLYSFQSD
660 670 680 690 700
KVTIVDHHSA TESFIKHMEN EYRCRGGCPA DWVWIVPPMS GSITPVFHQE
710 720 730 740 750
MLNYRLTPCF EYQPDPWNTH VWKGTNGTPT KRRAIGFKKL AEAVKFSAKL
760 770 780 790 800
MGQAMAKRVK ATILYATETG KSQAYAKTLC EIFKHAFDAK VMSMEEYDIV
810 820 830 840 850
HLEHETLVLV VTSTFGNGDP PENGEKFRCA LMEMRHPNSL QEERKSYKVR
860 870 880 890 900
FNSVSSYSDS RKSSGDGPDV RDHFESAGPL ANVRFSVFGL GSRAYPHFCA
910 920 930 940 950
FGHAVDTLLE ELGGERILKM REGDELCGQE EAFRTWAKKV FKAACDVFCV
960 970 980 990 1000
GDDVNIEKAN NSLISNDRSW KRNKFRLTYV AEAPGLTQGL SSVHKKRVSA
1010 1020 1030 1040 1050
ARLLSRQNLQ SPKSSRSTIF VRLHTNGSQE LQYQPGDHLG VFPGNHEDLV
1060 1070 1080 1090 1100
NALIERLEDA PPANQMVKVE LLEERNTALG VISNWKDEPR LPPCTVFQAF
1110 1120 1130 1140 1150
KYYLDITTPP TPLQLQQFAS LASNEKEKQR LLVLSKGLQE YEEWKWGKNP
1160 1170 1180 1190 1200
TIVEVLEEFP SIQMPATLLL TQLSLLQPRY YSISSSPDMY PDEVHLTVAI
1210 1220 1230 1240 1250
VSYHTRDGEG PIHHGVCSSW LNRIPADEVV PCFVRGAPSF RLPRNPQVPC
1260 1270 1280 1290 1300
ILVGPGTAFA PFRSFWQQRQ FDIQHKGMSP CPMVLVFGCR QSKIDHIYRE
1310 1320 1330 1340 1350
EALQAKNKGV FRELYTAYSR EPDKPKKYVQ DILQEQLAEQ VYRALKEQGG
1360 1370 1380 1390 1400
HIYVCGDVTM AADVLKAVQR IMAQQGKLSA EDAGVFISRL RDDNRYHEDI
1410 1420 1430
FGVTLRTYEV TNRLRSESIA FIEESKKDTD EVFSS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U91584 mRNA Translation: AAB68663.1 |
RefSeqi | NP_001075854.1, NM_001082385.1 |
Genome annotation databases
GeneIDi | 100009243 |
KEGGi | ocu:100009243 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U91584 mRNA Translation: AAB68663.1 |
RefSeqi | NP_001075854.1, NM_001082385.1 |
3D structure databases
BMRBi | O19132 |
SMRi | O19132 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9986.ENSOCUP00000020745 |
Proteomic databases
PRIDEi | O19132 |
Genome annotation databases
GeneIDi | 100009243 |
KEGGi | ocu:100009243 |
Organism-specific databases
CTDi | 4842 |
Phylogenomic databases
eggNOGi | KOG1158, Eukaryota |
InParanoidi | O19132 |
OrthoDBi | 90349at2759 |
Family and domain databases
Gene3Di | 1.20.990.10, 1 hit 2.30.42.10, 1 hit 3.40.50.360, 1 hit 3.40.50.80, 1 hit 3.90.1230.10, 1 hit 3.90.340.10, 1 hit 3.90.440.10, 1 hit |
InterProi | View protein in InterPro IPR003097, CysJ-like_FAD-binding IPR017927, FAD-bd_FR_type IPR001094, Flavdoxin-like IPR008254, Flavodoxin/NO_synth IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR029039, Flavoprotein-like_sf IPR039261, FNR_nucleotide-bd IPR023173, NADPH_Cyt_P450_Rdtase_alpha IPR044943, NOS_dom_1 IPR044940, NOS_dom_2 IPR044944, NOS_dom_3 IPR012144, NOS_euk IPR004030, NOS_N IPR036119, NOS_N_sf IPR001433, OxRdtase_FAD/NAD-bd IPR001478, PDZ IPR036034, PDZ_sf IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00667, FAD_binding_1, 1 hit PF00258, Flavodoxin_1, 1 hit PF00175, NAD_binding_1, 1 hit PF02898, NO_synthase, 1 hit PF00595, PDZ, 1 hit |
PIRSFi | PIRSF000333, NOS, 1 hit |
PRINTSi | PR00369, FLAVODOXIN PR00371, FPNCR |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit SSF52218, SSF52218, 1 hit SSF52343, SSF52343, 1 hit SSF56512, SSF56512, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit PS50902, FLAVODOXIN_LIKE, 1 hit PS60001, NOS, 1 hit PS50106, PDZ, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NOS1_RABIT | |
Accessioni | O19132Primary (citable) accession number: O19132 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 157 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families