UniProtKB - O19001 (CDN1B_FELCA)
Cyclin-dependent kinase inhibitor 1B
CDKN1B
Functioni
GO - Molecular functioni
- chaperone binding Source: GO_Central
- cyclin binding Source: Ensembl
- cyclin-dependent protein serine/threonine kinase inhibitor activity Source: UniProtKB
- protein-containing complex binding Source: Ensembl
- protein kinase binding Source: Ensembl
- protein phosphatase binding Source: Ensembl
GO - Biological processi
- autophagic cell death Source: Ensembl
- cell cycle arrest Source: GO_Central
- cellular response to antibiotic Source: Ensembl
- cellular response to lithium ion Source: Ensembl
- cellular response to organic cyclic compound Source: Ensembl
- G1/S transition of mitotic cell cycle Source: GO_Central
- heart development Source: Ensembl
- inner ear development Source: Ensembl
- mitotic cell cycle arrest Source: Ensembl
- negative regulation of apoptotic process Source: Ensembl
- negative regulation of cardiac muscle tissue regeneration Source: Ensembl
- negative regulation of cell growth Source: Ensembl
- negative regulation of cell population proliferation Source: GO_Central
- negative regulation of cellular component movement Source: Ensembl
- negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
- negative regulation of epithelial cell proliferation involved in prostate gland development Source: Ensembl
- negative regulation of mitotic cell cycle Source: GO_Central
- negative regulation of phosphorylation Source: GO_Central
- negative regulation of transcription, DNA-templated Source: Ensembl
- negative regulation of vascular associated smooth muscle cell proliferation Source: Ensembl
- Notch signaling pathway Source: Ensembl
- placenta development Source: Ensembl
- positive regulation of cell death Source: Ensembl
- positive regulation of cell population proliferation Source: Ensembl
- positive regulation of microtubule polymerization Source: Ensembl
- positive regulation of protein catabolic process Source: Ensembl
- potassium ion transport Source: Ensembl
- regulation of cell cycle G1/S phase transition Source: Ensembl
- regulation of exit from mitosis Source: Ensembl
- regulation of lens fiber cell differentiation Source: Ensembl
- sensory perception of sound Source: Ensembl
Keywordsi
Molecular function | Protein kinase inhibitor |
Biological process | Cell cycle |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclin-dependent kinase inhibitor 1BAlternative name(s): Cyclin-dependent kinase inhibitor p27 p27Kip1 |
Gene namesi | Name:CDKN1B Synonyms:KIP1 |
Organismi | Felis catus (Cat) (Felis silvestris catus) |
Taxonomic identifieri | 9685 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis |
Proteomesi |
|
Subcellular locationi
Nucleus
- Nucleus By similarity
Endosome
- Endosome By similarity
Other locations
- Cytoplasm By similarity
Note: Nuclear and cytoplasmic in quiescent cells. AKT- or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89 (By similarity). Colocalizes at the endosome with SNX6; this leads to lysosomal degradation (By similarity).By similarity
Cytosol
- cytosol Source: Ensembl
Endosome
- endosome Source: UniProtKB-SubCell
Nucleus
- nucleoplasm Source: Ensembl
- nucleus Source: GO_Central
Other locations
- Cul4A-RING E3 ubiquitin ligase complex Source: Ensembl
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, Endosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000190083 | 1 – 198 | Cyclin-dependent kinase inhibitor 1BAdd BLAST | 198 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | Phosphoserine; by UHMK1By similarity | 1 | |
Modified residuei | 74 | Phosphotyrosine; by SRCBy similarity | 1 | |
Modified residuei | 88 | Phosphotyrosine; by ABL, LYN and SRCBy similarity | 1 | |
Modified residuei | 89 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 157 | Phosphothreonine; by CaMK1, PKB/AKT1 and PIM1By similarity | 1 | |
Modified residuei | 170 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 187 | Phosphothreonine; by PKB/AKT1, CDK1 and CDK2By similarity | 1 | |
Modified residuei | 198 | Phosphothreonine; by CaMK1, PKB/AKT1, RPS6KA1, RPS6KA3 and PIM1By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationExpressioni
Gene expression databases
Bgeei | ENSFCAG00000022816, Expressed in spleen and 10 other tissues |
Interactioni
Subunit structurei
Forms a ternary complex composed of CCNE1, CDK2 and CDKN1B.
Interacts directly with CCNE1; the interaction is inhibited by CDK2-dependent phosphorylation on Thr-187.
Interacts with COPS5, subunit of the COP9 signalosome complex; the interaction leads to CDKN1B degradation.
Interacts with NUP50; the interaction leads to nuclear import and degradation of phosphorylated CDKN1B.
Interacts with CCND1 and SNX6 (By similarity).
Interacts (Thr-198-phosphorylated form) with 14-3-3 proteins, binds strongly YWHAQ, weakly YWHAE and YWHAH, but not YWHAB nor YWHAZ; the interaction with YWHAQ results in translocation to the cytoplasm.
Interacts with AKT1 and LYN; the interactions lead to cytoplasmic mislocation, phosphorylation of CDKN1B and inhibition of cell cycle arrest.
Forms a ternary complex with CCNA2 and CDK2; CDKN1B inhibits the kinase activity of CDK2 through conformational rearrangements.
Interacts (unphosphorylated form) with CDK2.
Forms a complex with CDK2 and SPDYA, but does not directly interact with SPDYA.
Forms a ternary complex composed of cyclin D, CDK4 and CDKN1B.
Interacts (phosphorylated on Tyr-88 and Tyr-89) with CDK4; the interaction is required for cyclin D and CDK4 complex assembly, induces nuclear translocation and activates the CDK4 kinase activity.
Interacts with GRB2.
Interacts with PIM1.
Identified in a complex with SKP1, SKP2 and CKS1B.
Interacts with UHMK1; the interaction leads to cytoplasmic mislocation, phosphorylation of CDKN1B and inhibition of cell cycle arrest.
Interacts also with CDK1. Dephosphorylated on Thr-187 by PPM1H, leading to CDKN1B stability (By similarity).
By similarityGO - Molecular functioni
- chaperone binding Source: GO_Central
- cyclin binding Source: Ensembl
- protein kinase binding Source: Ensembl
- protein phosphatase binding Source: Ensembl
Protein-protein interaction databases
STRINGi | 9685.ENSFCAP00000023343 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 51 – 91 | Interaction with CDK2By similarityAdd BLAST | 41 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 153 – 169 | Nuclear localization signalSequence analysisAdd BLAST | 17 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4743, Eukaryota |
GeneTreei | ENSGT00940000159852 |
HOGENOMi | CLU_077692_2_0_1 |
InParanoidi | O19001 |
OMAi | EGRYEWQ |
OrthoDBi | 1595421at2759 |
TreeFami | TF101038 |
Family and domain databases
InterProi | View protein in InterPro IPR003175, CDI IPR029843, CDKN1B |
PANTHERi | PTHR10265:SF9, PTHR10265:SF9, 1 hit |
Pfami | View protein in Pfam PF02234, CDI, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSNVRVSNGS PSLERMDARQ AEYPKPSACR NLFGPVNHEE LTRDLEKHCR
60 70 80 90 100
DMEEASQRKW NFDFQNHKPL EGKYEWQEVE KGSLPEFYYR PPRPPKGACK
110 120 130 140 150
VPAQESQDVS GNRQAVPLIG SQANTEDTHL VDQKTDTSDN QTGLAEQCPG
160 170 180 190
IRKRPATDDS SPQNKRANRT EENVSDGSPN AGSVEQTPKK PGLRRRQT
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84649 mRNA Translation: BAA23167.1 |
RefSeqi | NP_001009870.1, NM_001009870.1 |
Genome annotation databases
Ensembli | ENSFCAT00000058117; ENSFCAP00000044526; ENSFCAG00000043547 |
GeneIDi | 493958 |
KEGGi | fca:493958 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D84649 mRNA Translation: BAA23167.1 |
RefSeqi | NP_001009870.1, NM_001009870.1 |
3D structure databases
SMRi | O19001 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9685.ENSFCAP00000023343 |
Genome annotation databases
Ensembli | ENSFCAT00000058117; ENSFCAP00000044526; ENSFCAG00000043547 |
GeneIDi | 493958 |
KEGGi | fca:493958 |
Organism-specific databases
CTDi | 1027 |
Phylogenomic databases
eggNOGi | KOG4743, Eukaryota |
GeneTreei | ENSGT00940000159852 |
HOGENOMi | CLU_077692_2_0_1 |
InParanoidi | O19001 |
OMAi | EGRYEWQ |
OrthoDBi | 1595421at2759 |
TreeFami | TF101038 |
Gene expression databases
Bgeei | ENSFCAG00000022816, Expressed in spleen and 10 other tissues |
Family and domain databases
InterProi | View protein in InterPro IPR003175, CDI IPR029843, CDKN1B |
PANTHERi | PTHR10265:SF9, PTHR10265:SF9, 1 hit |
Pfami | View protein in Pfam PF02234, CDI, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDN1B_FELCA | |
Accessioni | O19001Primary (citable) accession number: O19001 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families