UniProtKB - O18963 (CP2E1_BOVIN)
Cytochrome P450 2E1
CYP2E1
Functioni
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
By similarityCatalytic activityi
- an organic molecule + O2 + reduced [NADPH—hemoprotein reductase] = an alcohol + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.1By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z)-eicosatrienoate + O2 + reduced [NADPH—hemoprotein reductase] = 19-hydroxy-(5Z,8Z,11Z)-eicosatrienoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + O2 + reduced [NADPH—hemoprotein reductase] = 19-hydroxy-(5Z,8Z,11Z,14Z,17Z)-eicosapentaenoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- (4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + O2 + reduced [NADPH—hemoprotein reductase] = 21-hydroxy-(4Z,7Z,10Z,13Z,16Z,19Z)-docosahexaenoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- dodecanoate + O2 + reduced [NADPH—hemoprotein reductase] = 11-hydroxydodecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- O2 + reduced [NADPH—hemoprotein reductase] + tetradecanoate = 13-hydroxytetradecanoate + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:1.14.13.n7By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Activity regulationi
: fatty acid metabolism Pathwayi
This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.By similarityView all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 437 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- arachidonic acid epoxygenase activity Source: GO_Central
- aromatase activity Source: UniProtKB-EC
- heme binding Source: UniProtKB
- Hsp70 protein binding Source: UniProtKB
- Hsp90 protein binding Source: UniProtKB
- iron ion binding Source: InterPro
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
- steroid hydroxylase activity Source: GO_Central
GO - Biological processi
- epoxygenase P450 pathway Source: GO_Central
- organic acid metabolic process Source: GO_Central
- xenobiotic metabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 2E1 (EC:1.14.14.1By similarity)Alternative name(s): 4-nitrophenol 2-hydroxylase (EC:1.14.13.n7By similarity) CYPIIE1 |
Gene namesi | Name:CYP2E1 Synonyms:CYP2E |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
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Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Peripheral membrane protein By similarity
- Microsome membrane By similarity; Peripheral membrane protein By similarity
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity
Note: Post-translationally targeted to mitochondria. TOMM70 is required for the translocation across the mitochondrial outer membrane. After translocation into the matrix, associates with the inner membrane as a membrane extrinsic protein.By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Mitochondrion
- mitochondrial inner membrane Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- intracellular membrane-bounded organelle Source: GO_Central
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051750 | 1 – 495 | Cytochrome P450 2E1Add BLAST | 495 |
Proteomic databases
PaxDbi | O18963 |
PeptideAtlasi | O18963 |
Interactioni
Subunit structurei
Interacts with chaperones HSP70 and HSP90; this interaction is required for initial targeting to mitochondria.
By similarityGO - Molecular functioni
- Hsp70 protein binding Source: UniProtKB
- Hsp90 protein binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000024437 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 298 – 303 | Substrate bindingBy similarity | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
InParanoidi | O18963 |
OrthoDBi | 702827at2759 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008070, Cyt_P450_E_grp-I_CYP2E-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01687, EP450ICYP2E PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAALGITVAL LVWMATLLFI SIWKHIYSSW KLPPGPFPLP IIGNLLQLDI
60 70 80 90 100
KNIPKSFTRL AERYGPVFTL YLGSQRAVVV HGYKPVKEVL LDYKNEFSGR
110 120 130 140 150
GENPGFQMHK NNGIIFNNGS TWRDTRRFSL TTLRDLGMGK QGNEQRIQRE
160 170 180 190 200
AHFLLEVLRK TQGQPFDPTF VVGFAPYNVI SDILFHKRFD YKDQTSLRLM
210 220 230 240 250
SLFNENFYLL SSPWIQLYNN FPDYLQYLPG SHRKLLKNVS EVKSYALERV
260 270 280 290 300
KDHQKSLEPS CPRGFLDTML IEMAKERHSV DPMYTLENIA VTVADLLFAG
310 320 330 340 350
TETTSTTLRY GLLILMKYPE VEEKLHEEID RVIGPSRIPA VKDRLDMPYL
360 370 380 390 400
DAVVHEIQRF IDLLPSNLLH EATQDTVFRG YVIPKGTVVI PTLDSVLHDR
410 420 430 440 450
QEFPEPEKFK PEHFLNENGK FKYSDHFKAF SAGKRVCVGE GLARMELFLL
460 470 480 490
LAAILQHFNL KSLVDPKDID LSPIAIGFGK IPPRYKLCLI PRSKV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ001715 mRNA Translation: CAA04948.1 DQ058608 mRNA Translation: AAY83882.1 DQ058605 Genomic DNA Translation: AAY83880.1 |
RefSeqi | NP_776955.2, NM_174530.3 |
Genome annotation databases
GeneIDi | 282213 |
KEGGi | bta:282213 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ001715 mRNA Translation: CAA04948.1 DQ058608 mRNA Translation: AAY83882.1 DQ058605 Genomic DNA Translation: AAY83880.1 |
RefSeqi | NP_776955.2, NM_174530.3 |
3D structure databases
SMRi | O18963 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000024437 |
Proteomic databases
PaxDbi | O18963 |
PeptideAtlasi | O18963 |
Genome annotation databases
GeneIDi | 282213 |
KEGGi | bta:282213 |
Organism-specific databases
CTDi | 1571 |
Phylogenomic databases
eggNOGi | KOG0156, Eukaryota |
InParanoidi | O18963 |
OrthoDBi | 702827at2759 |
Enzyme and pathway databases
UniPathwayi | UPA00199 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR008070, Cyt_P450_E_grp-I_CYP2E-like IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR01687, EP450ICYP2E PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CP2E1_BOVIN | |
Accessioni | O18963Primary (citable) accession number: O18963 Secondary accession number(s): Q4PS78 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families