UniProtKB - O18927 (MMP13_HORSE)
Collagenase 3
MMP13
Functioni
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion (By similarity).
By similarityCofactori
Protein has several cofactor binding sites:- Ca2+By similarityNote: Can bind about 5 Ca2+ ions per subunit.By similarity
- Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 97 | Zinc 2; in inhibited formBy similarity | 1 | |
Metal bindingi | 129 | Calcium 1By similarity | 1 | |
Metal bindingi | 163 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 173 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 175 | Zinc 1By similarity | 1 | |
Metal bindingi | 180 | Calcium 3By similarity | 1 | |
Metal bindingi | 181 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 183 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 185 | Calcium 3; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 188 | Zinc 1; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 195 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 197 | Calcium 2; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 199 | Calcium 2By similarity | 1 | |
Metal bindingi | 201 | Zinc 1; via pros nitrogenBy similarity | 1 | |
Metal bindingi | 203 | Calcium 3By similarity | 1 | |
Metal bindingi | 204 | Calcium 1By similarity | 1 | |
Metal bindingi | 206 | Calcium 1; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 206 | Calcium 3By similarity | 1 | |
Metal bindingi | 223 | Zinc 2; via tele nitrogen; catalyticBy similarity | 1 | |
Active sitei | 224 | PROSITE-ProRule annotation | 1 | |
Metal bindingi | 227 | Zinc 2; via tele nitrogen; catalyticBy similarity | 1 | |
Metal bindingi | 233 | Zinc 2; via tele nitrogen; catalyticBy similarity | 1 | |
Metal bindingi | 241 | Zinc 2; via carbonyl oxygen; catalyticBy similarity | 1 | |
Metal bindingi | 292 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 294 | Calcium 5; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 336 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 338 | Calcium 5; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 384 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 386 | Calcium 5; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 433 | Calcium 4; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 435 | Calcium 5; via carbonyl oxygenBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: UniProtKB
- collagen binding Source: UniProtKB
- endopeptidase activity Source: UniProtKB
- metalloendopeptidase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- bone morphogenesis Source: UniProtKB
- collagen catabolic process Source: UniProtKB
- extracellular matrix disassembly Source: UniProtKB
- extracellular matrix organization Source: GO_Central
Keywordsi
Molecular function | Hydrolase, Metalloprotease, Protease |
Biological process | Collagen degradation |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.4.24.B4, 2120 |
Protein family/group databases
MEROPSi | M10.013 |
Names & Taxonomyi
Protein namesi | Recommended name: Collagenase 3 (EC:3.4.24.-)Alternative name(s): Matrix metalloproteinase-13 Short name: MMP-13 |
Gene namesi | Name:MMP13 |
Organismi | Equus caballus (Horse) |
Taxonomic identifieri | 9796 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › |
Proteomesi |
|
Subcellular locationi
Extracellular region or secreted
- extracellular matrix Curated
- Secreted By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Other locations
- extracellular matrix Source: InterPro
Keywords - Cellular componenti
Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
PropeptideiPRO_0000028786 | 20 – 104 | Activation peptideSequence analysisAdd BLAST | 85 | |
ChainiPRO_0000028787 | 105 – 472 | Collagenase 3Add BLAST | 368 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 118 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 153 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 159 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 285 ↔ 472 | By similarity | ||
Modified residuei | 367 | Phosphotyrosine; by PKDCCBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, Phosphoprotein, ZymogenProteomic databases
PaxDbi | O18927 |
Expressioni
Tissue specificityi
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 282 – 331 | Hemopexin 1Add BLAST | 50 | |
Repeati | 332 – 378 | Hemopexin 2Add BLAST | 47 | |
Repeati | 380 – 428 | Hemopexin 3Add BLAST | 49 | |
Repeati | 429 – 472 | Hemopexin 4Add BLAST | 44 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 177 – 247 | Interaction with TIMP2By similarityAdd BLAST | 71 | |
Regioni | 269 – 472 | Interaction with collagenBy similarityAdd BLAST | 204 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 95 – 102 | Cysteine switchBy similarity | 8 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, SignalPhylogenomic databases
InParanoidi | O18927 |
OrthoDBi | 1075463at2759 |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR028711, Collagenase_3 IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf |
PANTHERi | PTHR10201:SF165, PTHR10201:SF165, 1 hit |
Pfami | View protein in Pfam PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MHPGVLAAFL FLSWTRCWSL PVPNDDDDDD DMSEEDFQLA ERYLKSYYYP
60 70 80 90 100
LNPAGILKKT AANSVVDRLR EMQSFFGLEV TGKLDDNTLD IMKKPRCGVP
110 120 130 140 150
DVGEYNVFPR TLKWPKMNLT YRIVNYTPDL THSEVEKAFK KAFKVWSDVT
160 170 180 190 200
PLNFTRLYNG TADIMISFGT KEHGDFYPFD GPSGLLAHAF PPGPNYGGDA
210 220 230 240 250
HFDDDETWTS SSKGYNLFLV AAHEFGHSLG LDHSKDPGAL MFPIYTYTGK
260 270 280 290 300
SHFVLPDDDV QGIQYLYGPG DEDPNPKHPK TPDKCDPSLS LDAITSLRGE
310 320 330 340 350
TMVFKDRFFW RLHPQLVDAE LFLTKSFWPE LPNRIDAAYE HPSKDLIFIF
360 370 380 390 400
RGRKFWALNG YDILEGYPQK ISELGFPKDV KKISAAVHFE DTGKTLFFSG
410 420 430 440 450
NQVWRYDDTN RMMDKDYPRL IEEDFPGIGD KVDAVYEKNG YIYFFNGPIQ
460 470
FEYSIWSNRI VRVMPTNSLL WC
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF034087 mRNA Translation: AAB86893.1 |
RefSeqi | NP_001075273.1, NM_001081804.1 |
Genome annotation databases
GeneIDi | 100009711 |
KEGGi | ecb:100009711 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF034087 mRNA Translation: AAB86893.1 |
RefSeqi | NP_001075273.1, NM_001081804.1 |
3D structure databases
SMRi | O18927 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9796.ENSECAP00000005084 |
Protein family/group databases
MEROPSi | M10.013 |
Proteomic databases
PaxDbi | O18927 |
Genome annotation databases
GeneIDi | 100009711 |
KEGGi | ecb:100009711 |
Organism-specific databases
CTDi | 4322 |
Phylogenomic databases
InParanoidi | O18927 |
OrthoDBi | 1075463at2759 |
Enzyme and pathway databases
BRENDAi | 3.4.24.B4, 2120 |
Family and domain databases
CDDi | cd00094, HX, 1 hit cd04278, ZnMc_MMP, 1 hit |
Gene3Di | 2.110.10.10, 1 hit 3.40.390.10, 1 hit |
InterProi | View protein in InterPro IPR028711, Collagenase_3 IPR000585, Hemopexin-like_dom IPR036375, Hemopexin-like_dom_sf IPR018487, Hemopexin-like_repeat IPR018486, Hemopexin_CS IPR033739, M10A_MMP IPR024079, MetalloPept_cat_dom_sf IPR001818, Pept_M10_metallopeptidase IPR021190, Pept_M10A IPR021158, Pept_M10A_Zn_BS IPR006026, Peptidase_Metallo IPR002477, Peptidoglycan-bd-like IPR036365, PGBD-like_sf |
PANTHERi | PTHR10201:SF165, PTHR10201:SF165, 1 hit |
Pfami | View protein in Pfam PF00045, Hemopexin, 4 hits PF00413, Peptidase_M10, 1 hit PF01471, PG_binding_1, 1 hit |
PIRSFi | PIRSF001191, Peptidase_M10A_matrix, 1 hit |
PRINTSi | PR00138, MATRIXIN |
SMARTi | View protein in SMART SM00120, HX, 4 hits SM00235, ZnMc, 1 hit |
SUPFAMi | SSF47090, SSF47090, 1 hit SSF50923, SSF50923, 1 hit |
PROSITEi | View protein in PROSITE PS00546, CYSTEINE_SWITCH, 1 hit PS00024, HEMOPEXIN, 1 hit PS51642, HEMOPEXIN_2, 4 hits PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MMP13_HORSE | |
Accessioni | O18927Primary (citable) accession number: O18927 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families