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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117CalciumBy similarity1
Metal bindingi168Calcium; via carbonyl oxygenBy similarity1
Metal bindingi177CalciumBy similarity1
Binding sitei205ChlorideBy similarity1
Active sitei207NucleophileBy similarity1
Metal bindingi211Calcium; via carbonyl oxygenBy similarity1
Active sitei244Proton donorBy similarity1
Binding sitei307ChlorideBy similarity1
Sitei309Transition state stabilizerBy similarity1
Binding sitei342ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-189085 Digestion of dietary carbohydrate

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Gene namesi
Name:Amyrel
ORF Names:CG8221
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0020506 Amyrel

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000000138220 – 493Alpha-amylase-related proteinAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi375 ↔ 381By similarity
Disulfide bondi417 ↔ 440Sequence analysis
Disulfide bondi447 ↔ 459By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiO18408
PRIDEiO18408

Expressioni

Tissue specificityi

Midgut and fat body.

Developmental stagei

Expressed during second and third larval instars, but not in the adult.

Gene expression databases

BgeeiFBgn0020506 Expressed in 6 organ(s), highest expression level in multi-cellular organism
ExpressionAtlasiO18408 baseline and differential
GenevisibleiO18408 DM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi62575, 5 interactors
IntActiO18408, 3 interactors
STRINGi7227.FBpp0086263

Structurei

3D structure databases

ProteinModelPortaliO18408
SMRiO18408
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
InParanoidiO18408
KOiK01176
OMAiQSWGTDW
OrthoDBiEOG091G07V6
PhylomeDBiO18408

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18408-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFKFALTLTL CLAGSLSLAQ HNPHWWGNRN TIVHLFEWKW SDIAQECESF
60 70 80 90 100
LGPRGFAGVQ VSPVNENILS AGRPWWERYQ PISYKLTTRS GNEEEFGDMV
110 120 130 140 150
RRCNDVGVRI YVDVLLNHMS GDFDGVAVGT AGTEAEPSKK SFPGVPYTAQ
160 170 180 190 200
DFHPTCEITD WNDRFQVQQC ELVGLKDLDQ SSDWVRSKLI EFLDHLIELG
210 220 230 240 250
VAGFRVDAAK HMASEDLEYI YSSLSNLNID HGFPHNSRPF IFQEVIDHGH
260 270 280 290 300
ETVSRDEYKD LGAVTEFRFS EEIGNAFRGN NALKWLQSWG TDWGFLPSGQ
310 320 330 340 350
ALTFVDNHDN QRDAGAVLNY KSPRQYKMAT AFHLAYPYGI SRVMSSFAFD
360 370 380 390 400
DHDTPPPQDA QERIISPEFD ADGACVNGWI CEHRWRQIYA MVGFKNAVRD
410 420 430 440 450
TEITGWWDNG DNQISFCRGN KGFLAINNNL YDLSQDLNTC LPAGTYCDVI
460 470 480 490
SGSLIDGSCT GKSVTVNENG YGYIHIGSDD FDGVLALHVD AKV
Length:493
Mass (Da):55,451
Last modified:January 11, 2011 - v2
Checksum:i3223298DAF7E634C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2F → S in AAD08845 (Ref. 2) Curated1
Sequence conflicti2F → S in AAD09147 (Ref. 3) Curated1
Sequence conflicti7T → A in AAD08845 (Ref. 2) Curated1
Sequence conflicti18L → V in AAD08845 (Ref. 2) Curated1
Sequence conflicti18L → V in AAD09147 (Ref. 3) Curated1
Sequence conflicti69L → I in AAD08845 (Ref. 2) Curated1
Sequence conflicti69L → I in AAD09147 (Ref. 3) Curated1
Sequence conflicti127A → V in AAD08845 (Ref. 2) Curated1
Sequence conflicti127A → V in AAD09147 (Ref. 3) Curated1
Sequence conflicti157E → V in AAD08845 (Ref. 2) Curated1
Sequence conflicti157E → V in AAD09147 (Ref. 3) Curated1
Sequence conflicti253V → D in AAD08845 (Ref. 2) Curated1
Sequence conflicti340I → T in AAD08845 (Ref. 2) Curated1
Sequence conflicti358Q → R in AAD08845 (Ref. 2) Curated1
Sequence conflicti466 – 467VN → MS in AAD08845 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69607 Genomic DNA Translation: AAD08845.1
AF022713 Genomic DNA Translation: AAD09147.2
AE013599 Genomic DNA Translation: AAF57971.1
AY052055 mRNA Translation: AAK93479.1
RefSeqiNP_477262.1, NM_057914.4
UniGeneiDm.4395

Genome annotation databases

EnsemblMetazoaiFBtr0087117; FBpp0086263; FBgn0020506
GeneIDi36863
KEGGidme:Dmel_CG8221
UCSCiCG8221-RA d. melanogaster

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69607 Genomic DNA Translation: AAD08845.1
AF022713 Genomic DNA Translation: AAD09147.2
AE013599 Genomic DNA Translation: AAF57971.1
AY052055 mRNA Translation: AAK93479.1
RefSeqiNP_477262.1, NM_057914.4
UniGeneiDm.4395

3D structure databases

ProteinModelPortaliO18408
SMRiO18408
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62575, 5 interactors
IntActiO18408, 3 interactors
STRINGi7227.FBpp0086263

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PaxDbiO18408
PRIDEiO18408

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087117; FBpp0086263; FBgn0020506
GeneIDi36863
KEGGidme:Dmel_CG8221
UCSCiCG8221-RA d. melanogaster

Organism-specific databases

CTDi36863
FlyBaseiFBgn0020506 Amyrel

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
GeneTreeiENSGT00390000002882
InParanoidiO18408
KOiK01176
OMAiQSWGTDW
OrthoDBiEOG091G07V6
PhylomeDBiO18408

Enzyme and pathway databases

ReactomeiR-DME-189085 Digestion of dietary carbohydrate

Miscellaneous databases

GenomeRNAii36863
PROiPR:O18408

Gene expression databases

BgeeiFBgn0020506 Expressed in 6 organ(s), highest expression level in multi-cellular organism
ExpressionAtlasiO18408 baseline and differential
GenevisibleiO18408 DM

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMYR_DROME
AccessioniPrimary (citable) accession number: O18408
Secondary accession number(s): Q9V7S3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 11, 2011
Last modified: September 12, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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Main funding by: National Institutes of Health

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