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Protein

Alpha-amylase 2

Gene

Amy58

Organism
Drosophila ananassae (Fruit fly)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi116CalciumBy similarity1
Metal bindingi165Calcium; via carbonyl oxygenBy similarity1
Metal bindingi174CalciumBy similarity1
Binding sitei202ChlorideBy similarity1
Active sitei204NucleophileBy similarity1
Metal bindingi208Calcium; via carbonyl oxygenBy similarity1
Active sitei241Proton donorBy similarity1
Binding sitei304ChlorideBy similarity1
Sitei306Transition state stabilizerBy similarity1
Binding sitei343ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 2 (EC:3.2.1.1By similarity)
Gene namesi
Name:Amy58
Synonyms:Amy2
ORF Names:GF18843
OrganismiDrosophila ananassae (Fruit fly)
Taxonomic identifieri7217 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000007801 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0261676 Dana\Amy58

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000000136119 – 494Alpha-amylase 2Add BLAST476

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO18345

Expressioni

Gene expression databases

BgeeiFBgn0261676 Expressed in 1 organ(s), highest expression level in adult organism

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7217.FBpp0122035

Structurei

3D structure databases

ProteinModelPortaliO18345
SMRiO18345
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
InParanoidiO18345
KOiK01176
OMAiHPWWEVY
OrthoDBiEOG091G07V6
PhylomeDBiO18345

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18345-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF NTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPYGYAGVQV SPVNENAVKD SRPWWERYQP ISYKLVTRSG NEEQFASMVR
110 120 130 140 150
RCNNVGVRIY VDVVFNHMAA DGGTYGTGGS TASPSSKSYP GVPFSSLDFN
160 170 180 190 200
PTCAISNYND ANQVRNCELV GLRDLNQGNS YVQEKIVEFL NHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLGVIYGS LKNLNTDHGF ESGAKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA ITEFRHSDSI GKAFRGKNQL QYLVNWGVSW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
TDTDQGPPTT DGQNIASPSF NSDNSCSGGW VCEHRWKQIY NMVGFRNAVG
410 420 430 440 450
SDAIQNWWDN GSNQIAFSRG SKGFVAFNND NYDLNSSVQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVTVGSD GRANISIGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,576
Last modified:January 11, 2011 - v2
Checksum:i3886599639E9126C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25A → P in AAC79122 (PubMed:9618501).Curated1
Sequence conflicti63V → A in AAC79122 (PubMed:9618501).Curated1
Sequence conflicti128G → A in AAC79122 (PubMed:9618501).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53698 Genomic DNA Translation: AAC79122.1
CH902617 Genomic DNA Translation: EDV44149.1
AF238958 Genomic DNA Translation: AAG45312.1
AF238959 Genomic DNA Translation: AAG45313.1
AF238960 Genomic DNA Translation: AAG45314.1
AF238961 Genomic DNA Translation: AAG45315.1
AF238962 Genomic DNA Translation: AAG45316.1
AF238963 Genomic DNA Translation: AAG45317.1
AF238964 Genomic DNA Translation: AAG45318.1
AF238965 Genomic DNA Translation: AAG45319.1
AF238966 Genomic DNA Translation: AAG45320.1
AF238967 Genomic DNA Translation: AAG45321.1
AF238968 Genomic DNA Translation: AAG45322.1
AF238969 Genomic DNA Translation: AAG45323.1
AF238970 Genomic DNA Translation: AAG45324.1
AF238971 Genomic DNA Translation: AAG45325.1
AF238972 Genomic DNA Translation: AAG45326.1
AF238973 Genomic DNA Translation: AAG45327.1
AF238974 Genomic DNA Translation: AAG45328.1
AF238975 Genomic DNA Translation: AAG45329.1
AF238976 Genomic DNA Translation: AAG45330.1
AF238977 Genomic DNA Translation: AAG45331.1
RefSeqiXP_001955588.1, XM_001955552.2

Genome annotation databases

EnsemblMetazoaiFBtr0123543; FBpp0122035; FBgn0261676
GeneIDi6501610
KEGGidan:Dana_GF18843

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53698 Genomic DNA Translation: AAC79122.1
CH902617 Genomic DNA Translation: EDV44149.1
AF238958 Genomic DNA Translation: AAG45312.1
AF238959 Genomic DNA Translation: AAG45313.1
AF238960 Genomic DNA Translation: AAG45314.1
AF238961 Genomic DNA Translation: AAG45315.1
AF238962 Genomic DNA Translation: AAG45316.1
AF238963 Genomic DNA Translation: AAG45317.1
AF238964 Genomic DNA Translation: AAG45318.1
AF238965 Genomic DNA Translation: AAG45319.1
AF238966 Genomic DNA Translation: AAG45320.1
AF238967 Genomic DNA Translation: AAG45321.1
AF238968 Genomic DNA Translation: AAG45322.1
AF238969 Genomic DNA Translation: AAG45323.1
AF238970 Genomic DNA Translation: AAG45324.1
AF238971 Genomic DNA Translation: AAG45325.1
AF238972 Genomic DNA Translation: AAG45326.1
AF238973 Genomic DNA Translation: AAG45327.1
AF238974 Genomic DNA Translation: AAG45328.1
AF238975 Genomic DNA Translation: AAG45329.1
AF238976 Genomic DNA Translation: AAG45330.1
AF238977 Genomic DNA Translation: AAG45331.1
RefSeqiXP_001955588.1, XM_001955552.2

3D structure databases

ProteinModelPortaliO18345
SMRiO18345
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7217.FBpp0122035

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PRIDEiO18345

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0123543; FBpp0122035; FBgn0261676
GeneIDi6501610
KEGGidan:Dana_GF18843

Organism-specific databases

FlyBaseiFBgn0261676 Dana\Amy58

Phylogenomic databases

eggNOGiKOG2212 Eukaryota
COG0366 LUCA
InParanoidiO18345
KOiK01176
OMAiHPWWEVY
OrthoDBiEOG091G07V6
PhylomeDBiO18345

Gene expression databases

BgeeiFBgn0261676 Expressed in 1 organ(s), highest expression level in adult organism

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMY2_DROAN
AccessioniPrimary (citable) accession number: O18345
Secondary accession number(s): B3LZH0, Q9GN73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 11, 2011
Last modified: September 12, 2018
This is version 102 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  3. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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