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Protein

Eyes absent homolog 1

Gene

eya-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tyrosine protein phosphatase (By similarity). Acts probably as a transcription regulator in the embryonic and postembryonic development of several tissues including pharynx, vulva and gonads (PubMed:16154558). Required for the development of anterior tissues during late embryogenesis (PubMed:16154558). Together with ceh-34, required to specify the coelomocyte fate in embryonic and postembryonic precursors (PubMed:19427847). In the anterior part of the embryo, prevents apoptosis in cells that are not fated to die (PubMed:16154558). Together with ceh-34 activates proapoptotic factor egl-1 expression to promote motor neuron M4 sister cell apoptosis (PubMed:20713707). Also promotes apoptosis of I1 pharyngeal neuron sister cell (PubMed:20713707). Plays a role in locomotion and fertility (PubMed:16154558). May play a role in resistance to heat and oxidative stresses (PubMed:25108328).By similarity4 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei237NucleophileBy similarity1
Metal bindingi237MagnesiumBy similarity1
Active sitei239Proton donorBy similarity1
Metal bindingi239Magnesium; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein tyrosine phosphatase activity Source: WormBase
  • transcription coactivator activity Source: WormBase

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Hydrolase, Protein phosphatase
Biological processTranscription, Transcription regulation
LigandMagnesium, Metal-binding

Protein family/group databases

MoonDBiO17670 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Eyes absent homolog 11 PublicationUniRule annotation (EC:3.1.3.48UniRule annotation)
Gene namesi
Name:eya-11 PublicationImported
ORF Names:C49A1.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditinaRhabditomorphaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiC49A1.4a ; CE36113 ; WBGene00001377 ; eya-1
C49A1.4b ; CE15722 ; WBGene00001377 ; eya-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown results in severe lethal arrest at an early larval stage (PubMed:16154558). The pharyngeal structure is disorganized and the anterior tip is thinner (PubMed:16154558). The phenotype is more severe in a pax-6 (ju468) mutant background (PubMed:16154558). RNAi-mediated knockdown in L1 larvae causes a decrease in resistance to heat or oxidative stresses characterized by a reduced lifespan, reduced heat shock protein hsp-16.2 expression and increased reactive oxygen species (ROS) production (PubMed:25108328).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004434221 – 503Eyes absent homolog 1Add BLAST503

Proteomic databases

PaxDbiO17670

Expressioni

Tissue specificityi

Expressed in body wall muscles.1 Publication

Developmental stagei

Expression starts at the embryonic bean stage in several anterior cells and continues until hatching (PubMed:16154558). Between the 1.5-fold and pretzel embryonic stages, expressed in a subset of pharyngeal cells and several anterior body wall muscle cells (PubMed:16154558). Expressed in the M lineage-derived coelomocyte precursor cells M.dlpa and M.drpa and in the 6 differentiated coelomocytes throughout development (PubMed:19427847). Expressed in the M4 motor neuron sister cell (PubMed:20713707).3 Publications

Gene expression databases

BgeeiWBGene00001377 Expressed in 4 organ(s), highest expression level in pharyngeal muscle cell (C elegans)

Interactioni

Subunit structurei

Interacts (via C-terminus) with ceh-34 (via N-terminus).2 Publications

Binary interactionsi

Protein-protein interaction databases

DIPiDIP-26060N
IntActiO17670, 119 interactors
STRINGi6239.C49A1.4a

Structurei

3D structure databases

ProteinModelPortaliO17670
SMRiO17670
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi216 – 219Poly-LysSequence analysis4

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily. EYA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG410IY3F Eukaryota
ENOG410XZ0G LUCA
GeneTreeiENSGT00390000008860
InParanoidiO17670
KOiK15616
OMAiSMEKADY
OrthoDBiEOG091G0EW6
PhylomeDBiO17670

Family and domain databases

CDDicd02601 HAD_Eya, 1 hit
Gene3Di3.40.50.12350, 1 hit
InterProiView protein in InterPro
IPR006545 EYA_dom
IPR038102 EYA_dom_sf
IPR028472 EYA_fam
PANTHERiPTHR10190 PTHR10190, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform aImported (identifier: O17670-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MLPDSEGQKL KTFLLGTGTT LDPSLDPTGN SNFSMATTSD SSTIWTALPA
60 70 80 90 100
SQPGDKDIPT VDLAAISEAY GSTSSTTSLT SSVTSQYQYN SYPQYAMYTS
110 120 130 140 150
ANPANYYQQV TANLRAGTTA FPYSLTTPSY YGSYPVDYTS AAAAYQNPYY
160 170 180 190 200
TNLRGGTAAP YYNPLNATTA AAYASVASSV LGTDAVNLGT SSDGSTGVPS
210 220 230 240 250
TVTSFSLKEK KPKVSKKKKT GSCSPGDETY ARVFIWDIDD IAVISRNYLA
260 270 280 290 300
SVTHTNEFYA RAANSVSHLM ERIALNNFAD VNEFLEGDIT NIEDAVVDET
310 320 330 340 350
TMDSGPIDNL RGLDVMRRVA PKYSAFRQFY TENSTKNDVA GFKQEQNGFN
360 370 380 390 400
FELLERVGFG AREATELYQS AIQLQTLPNF GQRWPCAQRC MDLVVEKSKL
410 420 430 440 450
SAEKYANVVL SNDGLVLGAA QLMISGLNSS VPVENIYSIS KQGKESVFEK
460 470 480 490 500
IQSRFGKKCS FICITSGDTA NSAKRLNIPV WPLNSNTDLD KLYSALDNFL

LGG
Length:503
Mass (Da):54,522
Last modified:July 5, 2004 - v3
Checksum:i53720CB133525C5C
GO
Isoform bImported (identifier: O17670-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:469
Mass (Da):50,957
Checksum:i31AEE85B0522F711
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0593461 – 34Missing in isoform b. CuratedAdd BLAST34

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA Translation: CAB05707.2
BX284601 Genomic DNA Translation: CAI79139.1
PIRiT20047
RefSeqiNP_001021055.1, NM_001025884.4 [O17670-1]
NP_001021056.1, NM_001025885.2 [O17670-2]
UniGeneiCel.7534

Genome annotation databases

EnsemblMetazoaiC49A1.4a; C49A1.4a; WBGene00001377 [O17670-1]
C49A1.4b; C49A1.4b; WBGene00001377 [O17670-2]
GeneIDi173293
KEGGicel:CELE_C49A1.4
UCSCiC49A1.4a c. elegans [O17670-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA Translation: CAB05707.2
BX284601 Genomic DNA Translation: CAI79139.1
PIRiT20047
RefSeqiNP_001021055.1, NM_001025884.4 [O17670-1]
NP_001021056.1, NM_001025885.2 [O17670-2]
UniGeneiCel.7534

3D structure databases

ProteinModelPortaliO17670
SMRiO17670
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-26060N
IntActiO17670, 119 interactors
STRINGi6239.C49A1.4a

Protein family/group databases

MoonDBiO17670 Predicted

Proteomic databases

PaxDbiO17670

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC49A1.4a; C49A1.4a; WBGene00001377 [O17670-1]
C49A1.4b; C49A1.4b; WBGene00001377 [O17670-2]
GeneIDi173293
KEGGicel:CELE_C49A1.4
UCSCiC49A1.4a c. elegans [O17670-1]

Organism-specific databases

CTDi173293
WormBaseiC49A1.4a ; CE36113 ; WBGene00001377 ; eya-1
C49A1.4b ; CE15722 ; WBGene00001377 ; eya-1

Phylogenomic databases

eggNOGiENOG410IY3F Eukaryota
ENOG410XZ0G LUCA
GeneTreeiENSGT00390000008860
InParanoidiO17670
KOiK15616
OMAiSMEKADY
OrthoDBiEOG091G0EW6
PhylomeDBiO17670

Miscellaneous databases

PROiPR:O17670

Gene expression databases

BgeeiWBGene00001377 Expressed in 4 organ(s), highest expression level in pharyngeal muscle cell (C elegans)

Family and domain databases

CDDicd02601 HAD_Eya, 1 hit
Gene3Di3.40.50.12350, 1 hit
InterProiView protein in InterPro
IPR006545 EYA_dom
IPR038102 EYA_dom_sf
IPR028472 EYA_fam
PANTHERiPTHR10190 PTHR10190, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEYA1_CAEEL
AccessioniPrimary (citable) accession number: O17670
Secondary accession number(s): Q564S5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2018
Last sequence update: July 5, 2004
Last modified: November 7, 2018
This is version 129 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
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