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Entry version 144 (26 Feb 2020)
Sequence version 2 (19 Sep 2003)
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Protein

Vesicular acetylcholine transporter

Gene

VAChT

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in acetylcholine transport into synaptic vesicles.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processNeurotransmitter transport, Transport

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-DME-264642 Acetylcholine Neurotransmitter Release Cycle
R-DME-8856825 Cargo recognition for clathrin-mediated endocytosis
R-DME-8856828 Clathrin-mediated endocytosis

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Vesicular acetylcholine transporter
Short name:
VAChT
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:VAChT
ORF Names:CG32848
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiDrosophila melanogaster (Fruit fly)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri7227 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000803 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 3R

Organism-specific databases

Drosophila genome database

More...
FlyBasei
FBgn0270928 VAChT

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the subcellular compartment where each non-membrane region of a membrane-spanning protein is found.<p><a href='/help/topo_dom' target='_top'>More...</a></p>Topological domaini1 – 32CytoplasmicSequence analysisAdd BLAST32
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular%5Flocation%5Fsection">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Topological domaini54 – 98Lumenal, vesicleSequence analysisAdd BLAST45
Transmembranei99 – 119HelicalSequence analysisAdd BLAST21
Topological domaini120 – 125CytoplasmicSequence analysis6
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 154Lumenal, vesicleSequence analysis8
Transmembranei155 – 175HelicalSequence analysisAdd BLAST21
Topological domaini176 – 187CytoplasmicSequence analysisAdd BLAST12
Transmembranei188 – 208HelicalSequence analysisAdd BLAST21
Topological domaini209 – 215Lumenal, vesicleSequence analysis7
Transmembranei216 – 236HelicalSequence analysisAdd BLAST21
Topological domaini237 – 263CytoplasmicSequence analysisAdd BLAST27
Transmembranei264 – 284HelicalSequence analysisAdd BLAST21
Topological domaini285 – 299Lumenal, vesicleSequence analysisAdd BLAST15
Transmembranei300 – 320HelicalSequence analysisAdd BLAST21
Topological domaini321 – 330CytoplasmicSequence analysis10
Transmembranei331 – 351HelicalSequence analysisAdd BLAST21
Topological domaini352 – 355Lumenal, vesicleSequence analysis4
Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
Topological domaini377 – 387CytoplasmicSequence analysisAdd BLAST11
Transmembranei388 – 408HelicalSequence analysisAdd BLAST21
Topological domaini409 – 413Lumenal, vesicleSequence analysis5
Transmembranei414 – 434HelicalSequence analysisAdd BLAST21
Topological domaini435 – 578CytoplasmicSequence analysisAdd BLAST144

Keywords - Cellular componenti

Membrane

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001275261 – 578Vesicular acetylcholine transporterAdd BLAST578

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section specifies the position and type of each covalently attached glycan group (mono-, di-, or polysaccharide).<p><a href='/help/carbohyd' target='_top'>More...</a></p>Glycosylationi78N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi293N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O17444

PRoteomics IDEntifications database

More...
PRIDEi
O17444

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
FBgn0015323 Expressed in brain and 8 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...
ExpressionAtlasi
O17444 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...
Genevisiblei
O17444 DM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Protein interaction database and analysis system

More...
IntActi
O17444, 2 interactors

STRING: functional protein association networks

More...
STRINGi
7227.FBpp0083086

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi509 – 513Poly-Gln5
Compositional biasi549 – 563Poly-GlnAdd BLAST15

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG3764 Eukaryota
ENOG410XW69 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000159449

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_001265_10_9_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O17444

KEGG Orthology (KO)

More...
KOi
K14636

Identification of Orthologs from Complete Genome Data

More...
OMAi
DPPDKEY

Database of Orthologous Groups

More...
OrthoDBi
956763at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O17444

Family and domain databases

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR004734 Multidrug-R

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF07690 MFS_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF103473 SSF103473, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00880 2_A_01_02, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50850 MFS, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O17444-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASFQIPVIN LEVREVKDIV WEKIQEPVNQ RRLILVIVSI ALLLDNMLYM
60 70 80 90 100
VIVPIIPDYL REIGSFDDGP TPPPLRDNIT GKIIPVHHDH HGQDSATGIL
110 120 130 140 150
FASKAIVQLM VNPFSGGLID KIGYDLPMMI GLTIMFFSTA VFACGSSYSV
160 170 180 190 200
LFFARSLQGA GSAFADTAGL AMIADRFTEE NERSQALGIA LAFISFGCLV
210 220 230 240 250
APPFGGALYQ FAGKEVPFLI LALVCLLDGL MLLLVMKPVK EAMKQSKDVQ
260 270 280 290 300
DQVIPIWRLL MDPYIAVCAG ALTMSNVALA FLEPTISLWM EDNMTTDNWK
310 320 330 340 350
IGMVWLPAFF PHVLGVVITV KMARKYPQHQ WLMAAGGLAL EGFSCFIIPF
360 370 380 390 400
CSGYKMLMLP ICVICFGIAL IDTALLPTLG YLVDVRYVSV YGSIYAIADI
410 420 430 440 450
SYSIAYAVGP IIAGGVVEAI GFTALNFLIA FSNLAYVPVL RKLRNIYDFK
460 470 480 490 500
PFENEANILM QDPPNKEYQT YVMHDQKPVE GGVKNHLEYG QQYQQEQETN
510 520 530 540 550
LDDQQYEYQQ QQQGYQQGYQ QDQGYQPGYQ EQGGSYAPQG QPRVANPFQQ
560 570
QQQQQQQQQQ QVQSRGPAAP ANPFRQGF
Length:578
Mass (Da):64,314
Last modified:September 19, 2003 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i4E73402CB934E4B5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti97T → S in AAB86609 (PubMed:9446576).Curated1
Sequence conflicti160A → V in AAB86609 (PubMed:9446576).Curated1
Sequence conflicti272L → M in AAB86609 (PubMed:9446576).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF030197 mRNA Translation: AAB86609.1
AE014297 Genomic DNA Translation: AAF55587.2

NCBI Reference Sequences

More...
RefSeqi
NP_477138.1, NM_057790.5

Genome annotation databases

Ensembl metazoan genome annotation project

More...
EnsemblMetazoai
FBtr0083671; FBpp0083086; FBgn0270928

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
42795

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
dme:Dmel_CG32848

UCSC genome browser

More...
UCSCi
CG32848-RA d. melanogaster

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030197 mRNA Translation: AAB86609.1
AE014297 Genomic DNA Translation: AAF55587.2
RefSeqiNP_477138.1, NM_057790.5

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

IntActiO17444, 2 interactors
STRINGi7227.FBpp0083086

Proteomic databases

PaxDbiO17444
PRIDEiO17444

Genome annotation databases

EnsemblMetazoaiFBtr0083671; FBpp0083086; FBgn0270928
GeneIDi42795
KEGGidme:Dmel_CG32848
UCSCiCG32848-RA d. melanogaster

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
42795
FlyBaseiFBgn0270928 VAChT

Phylogenomic databases

eggNOGiKOG3764 Eukaryota
ENOG410XW69 LUCA
GeneTreeiENSGT00940000159449
HOGENOMiCLU_001265_10_9_1
InParanoidiO17444
KOiK14636
OMAiDPPDKEY
OrthoDBi956763at2759
PhylomeDBiO17444

Enzyme and pathway databases

ReactomeiR-DME-264642 Acetylcholine Neurotransmitter Release Cycle
R-DME-8856825 Cargo recognition for clathrin-mediated endocytosis
R-DME-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
42795

Protein Ontology

More...
PROi
PR:O17444

Gene expression databases

BgeeiFBgn0015323 Expressed in brain and 8 other tissues
ExpressionAtlasiO17444 baseline and differential
GenevisibleiO17444 DM

Family and domain databases

InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR004734 Multidrug-R
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 1 hit
TIGRFAMsiTIGR00880 2_A_01_02, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiVACHT_DROME
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O17444
Secondary accession number(s): Q9VE42
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: September 19, 2003
Last modified: February 26, 2020
This is version 144 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
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