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Protein

Transcription factor MafG

Gene

MAFG

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves (PubMed:11154691). However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins, such as NFE2, NFE2L1 and NFE2L2, and recruiting them to specific DNA-binding sites (PubMed:8932385, PubMed:9421508, PubMed:11154691). Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NFE2L2 transcription factor (PubMed:11154691). Transcription factor, component of erythroid-specific transcription factor NFE2L2 (PubMed:11154691). Activates globin gene expression when associated with NFE2L2 (PubMed:11154691). May be involved in signal transduction of extracellular H+ (By similarity).By similarity3 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-983231 Factors involved in megakaryocyte development and platelet production
SIGNORiO15525

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor MafG
Alternative name(s):
V-maf musculoaponeurotic fibrosarcoma oncogene homolog G
hMAF
Gene namesi
Name:MAFG
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000197063.10
HGNCiHGNC:6781 MAFG
MIMi602020 gene
neXtProtiNX_O15525

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi53K → A: Abolishes acetylation. Has no effect on binding to NFE2 but impairs the DNA binding and transcriptional activities of NFE2; when associated with A-60; A-71 and A-76. 1 Publication1
Mutagenesisi60K → A: Abolishes acetylation. Has no effect on binding to NFE2 but impairs the DNA binding and transcriptional activities of NFE2; when associated with A-53; A-71 and A-76. 1 Publication1
Mutagenesisi71K → A: Abolishes acetylation. Has no effect on binding to NFE2 but impairs the DNA binding and transcriptional activities of NFE2; when associated with A-53; A-60; and A-76. 1 Publication1
Mutagenesisi76K → A: Abolishes acetylation. Has no effect on binding to NFE2 but impairs the DNA binding and transcriptional activities of NFE2; when associated with A-53; A-60 and A-71. 1 Publication1

Organism-specific databases

DisGeNETi4097
OpenTargetsiENSG00000197063
PharmGKBiPA30539

Polymorphism and mutation databases

BioMutaiMAFG

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765001 – 162Transcription factor MafGAdd BLAST162

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linki14Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei53N6-acetyllysine1 Publication1
Modified residuei60N6-acetyllysine1 Publication1
Modified residuei71N6-acetyllysine1 Publication1
Modified residuei76N6-acetyllysine1 Publication1
Modified residuei124PhosphoserineCombined sources1

Post-translational modificationi

Acetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.1 Publication
Sumoylation at Lys-14 is required for active transcriptional repression.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO15525
MaxQBiO15525
PaxDbiO15525
PeptideAtlasiO15525
PRIDEiO15525
ProteomicsDBi48725

PTM databases

iPTMnetiO15525
PhosphoSitePlusiO15525

Expressioni

Tissue specificityi

Highly expressed in skeletal muscle. Also expressed in heart and brain.

Gene expression databases

BgeeiENSG00000197063 Expressed in 208 organ(s), highest expression level in amniotic fluid
CleanExiHS_MAFG
ExpressionAtlasiO15525 baseline and differential
GenevisibleiO15525 HS

Organism-specific databases

HPAiCAB025573

Interactioni

Subunit structurei

Homodimer or heterodimer. Homodimerization leads to transcriptional repression. Forms high affinity heterodimers with members of the CNC-bZIP family such as NFE2, NFE2L1/NRF1, NFE2L2/NRF2 and NFE2L3/NRF3 (PubMed:8932385, PubMed:9421508, PubMed:11154691). Interacts with CREBBP; the interaction leads to acetylation of the basic region of MAFG and stimulation of NFE2 transcriptional activity through increased DNA binding.3 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi110272, 30 interactors
ELMiO15525
IntActiO15525, 17 interactors
MINTiO15525
STRINGi9606.ENSP00000350369

Structurei

3D structure databases

ProteinModelPortaliO15525
SMRiO15525
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 114bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni53 – 76Basic motifPROSITE-ProRule annotationAdd BLAST24
Regioni79 – 93Leucine-zipperPROSITE-ProRule annotationAdd BLAST15

Sequence similaritiesi

Belongs to the bZIP family. Maf subfamily.Curated

Phylogenomic databases

eggNOGiENOG410ISHW Eukaryota
ENOG4111HFT LUCA
GeneTreeiENSGT00550000074549
HOGENOMiHOG000045475
HOVERGENiHBG001725
InParanoidiO15525
KOiK09037
OMAiITASMGP
OrthoDBiEOG091G0H46
PhylomeDBiO15525
TreeFamiTF325689

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR028551 MafG
IPR008917 TF_DNA-bd_sf
IPR024874 Transciption_factor_Maf_fam
PANTHERiPTHR10129 PTHR10129, 1 hit
PTHR10129:SF15 PTHR10129:SF15, 1 hit
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O15525-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTPNKGNKA LKVKREPGEN GTSLTDEELV TMSVRELNQH LRGLSKEEIV
60 70 80 90 100
QLKQRRRTLK NRGYAASCRV KRVTQKEELE KQKAELQQEV EKLASENASM
110 120 130 140 150
KLELDALRSK YEALQTFART VARSPVAPAR GPLAAGLGPL VPGKVAATSV
160
ITIVKSKTDA RS
Length:162
Mass (Da):17,850
Last modified:January 1, 1998 - v1
Checksum:iE49F1FBA230F8D30
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I3L2F8I3L2F8_HUMAN
Transcription factor MafG
MAFG
98Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11514 mRNA Translation: CAA72284.1
U84249 mRNA Translation: AAC51737.1
AF059195 mRNA Translation: AAC14427.1
BC012327 mRNA Translation: AAH12327.1
CCDSiCCDS11793.1
RefSeqiNP_002350.1, NM_002359.3
NP_116100.2, NM_032711.3
XP_011521880.1, XM_011523578.1
UniGeneiHs.252229
Hs.744113

Genome annotation databases

EnsembliENST00000357736; ENSP00000350369; ENSG00000197063
ENST00000392366; ENSP00000376173; ENSG00000197063
GeneIDi4097
KEGGihsa:4097
UCSCiuc002kcm.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11514 mRNA Translation: CAA72284.1
U84249 mRNA Translation: AAC51737.1
AF059195 mRNA Translation: AAC14427.1
BC012327 mRNA Translation: AAH12327.1
CCDSiCCDS11793.1
RefSeqiNP_002350.1, NM_002359.3
NP_116100.2, NM_032711.3
XP_011521880.1, XM_011523578.1
UniGeneiHs.252229
Hs.744113

3D structure databases

ProteinModelPortaliO15525
SMRiO15525
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110272, 30 interactors
ELMiO15525
IntActiO15525, 17 interactors
MINTiO15525
STRINGi9606.ENSP00000350369

PTM databases

iPTMnetiO15525
PhosphoSitePlusiO15525

Polymorphism and mutation databases

BioMutaiMAFG

Proteomic databases

EPDiO15525
MaxQBiO15525
PaxDbiO15525
PeptideAtlasiO15525
PRIDEiO15525
ProteomicsDBi48725

Protocols and materials databases

DNASUi4097
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357736; ENSP00000350369; ENSG00000197063
ENST00000392366; ENSP00000376173; ENSG00000197063
GeneIDi4097
KEGGihsa:4097
UCSCiuc002kcm.4 human

Organism-specific databases

CTDi4097
DisGeNETi4097
EuPathDBiHostDB:ENSG00000197063.10
GeneCardsiMAFG
HGNCiHGNC:6781 MAFG
HPAiCAB025573
MIMi602020 gene
neXtProtiNX_O15525
OpenTargetsiENSG00000197063
PharmGKBiPA30539
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ISHW Eukaryota
ENOG4111HFT LUCA
GeneTreeiENSGT00550000074549
HOGENOMiHOG000045475
HOVERGENiHBG001725
InParanoidiO15525
KOiK09037
OMAiITASMGP
OrthoDBiEOG091G0H46
PhylomeDBiO15525
TreeFamiTF325689

Enzyme and pathway databases

ReactomeiR-HSA-983231 Factors involved in megakaryocyte development and platelet production
SIGNORiO15525

Miscellaneous databases

ChiTaRSiMAFG human
GeneWikiiMAFG
GenomeRNAii4097
PROiPR:O15525
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000197063 Expressed in 208 organ(s), highest expression level in amniotic fluid
CleanExiHS_MAFG
ExpressionAtlasiO15525 baseline and differential
GenevisibleiO15525 HS

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR004826 bZIP_Maf
IPR028551 MafG
IPR008917 TF_DNA-bd_sf
IPR024874 Transciption_factor_Maf_fam
PANTHERiPTHR10129 PTHR10129, 1 hit
PTHR10129:SF15 PTHR10129:SF15, 1 hit
PfamiView protein in Pfam
PF03131 bZIP_Maf, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
SUPFAMiSSF47454 SSF47454, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMAFG_HUMAN
AccessioniPrimary (citable) accession number: O15525
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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