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Protein

Calpain-5

Gene

CAPN5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81By similarity1
Active sitei252By similarity1
Active sitei284By similarity1

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: GO_Central

GO - Biological processi

  • proteolysis Source: GO_Central
  • signal transduction Source: ProtInc

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.B25 2681
ReactomeiR-HSA-1474228 Degradation of the extracellular matrix

Protein family/group databases

MEROPSiC02.011

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-5 (EC:3.4.22.-)
Alternative name(s):
Calpain htra-3
New calpain 3
Short name:
nCL-3
Gene namesi
Name:CAPN5
Synonyms:NCL3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000149260.14
HGNCiHGNC:1482 CAPN5
MIMi602537 gene
neXtProtiNX_O15484

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Involvement in diseasei

Vitreoretinopathy, neovascular inflammatory (VRNI)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autoimmune condition of the eye that sequentially mimics uveitis, retinitis pigmentosa, and proliferative diabetic retinopathy as it progresses to complete blindness. Patients present during the second or third decade of life with posterior uveitis and reduction of the electroretinogram b-wave. They become more symptomatic when cataracts, cystoid macular edema, and disk edema diminish visual acuity during the second stage. Severe vision loss begins during the third stage when proliferative retinal neovascularization and epiretinal membranes appear. There is an ongoing pigmentary retinal degeneration and peripheral visual field loss during all stages. In the fourth stage, proliferative vitreoretinopathy causes tractional retinal detachments at the macula and vitreous base. The fifth or end-stage disease is marked by phthisis.
See also OMIM:193235
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069277243R → L in VRNI; largely mislocalized to the cytoplasm whereas the wild-type protein is localized near the cell surface. 1 PublicationCorresponds to variant dbSNP:rs397514601EnsemblClinVar.1
Natural variantiVAR_069278244L → P in VRNI; largely mislocalized to the cytoplasm whereas the wild-type protein is localized near the cell surface. 1 PublicationCorresponds to variant dbSNP:rs397514602EnsemblClinVar.1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi726
MalaCardsiCAPN5
MIMi193235 phenotype
OpenTargetsiENSG00000149260
Orphaneti329211 Autosomal dominant neovascular inflammatory vitreoretinopathy
PharmGKBiPA26062

Polymorphism and mutation databases

BioMutaiCAPN5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077131 – 640Calpain-5Add BLAST640

Proteomic databases

EPDiO15484
MaxQBiO15484
PaxDbiO15484
PeptideAtlasiO15484
PRIDEiO15484
ProteomicsDBi48688

PTM databases

iPTMnetiO15484
PhosphoSitePlusiO15484
SwissPalmiO15484

Expressioni

Tissue specificityi

Expressed in many tissues. Strong expression in the photoreceptor cells of the retina, with a punctate pattern of labeling over the nuclei and inner segments with less expression along the other segments and outer plexiform layer.1 Publication

Gene expression databases

BgeeiENSG00000149260 Expressed in 171 organ(s), highest expression level in transverse colon
CleanExiHS_CAPN5
ExpressionAtlasiO15484 baseline and differential
GenevisibleiO15484 HS

Organism-specific databases

HPAiCAB033237

Interactioni

Protein-protein interaction databases

BioGridi107187, 3 interactors
IntActiO15484, 2 interactors
STRINGi9606.ENSP00000278559

Structurei

3D structure databases

ProteinModelPortaliO15484
SMRiO15484
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 343Calpain catalyticPROSITE-ProRule annotationAdd BLAST318
Domaini518 – 619C2Add BLAST102

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni344 – 496Domain IIIAdd BLAST153

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232036
HOVERGENiHBG001095
InParanoidiO15484
KOiK08574
PhylomeDBiO15484
TreeFamiTF314748

Family and domain databases

CDDicd04046 C2_Calpain, 1 hit
cd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR033884 C2_Calpain
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O15484-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFSCVKPYED QNYSALRRDC RRRKVLFEDP LFPATDDSLY YKGTPGPAVR
60 70 80 90 100
WKRPKGICED PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV
110 120 130 140 150
IPDWKEQEWD PEKPNAYAGI FHFHFWRFGE WVDVVIDDRL PTVNNQLIYC
160 170 180 190 200
HSNSRNEFWC ALVEKAYAKL AGCYQALDGG NTADALVDFT GGVSEPIDLT
210 220 230 240 250
EGDFANDETK RNQLFERMLK VHSRGGLISA SIKAVTAADM EARLACGLVK
260 270 280 290 300
GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERE WNGPWSDTSE
310 320 330 340 350
EWQKVSKSER EKMGVTVQDD GEFWMTFEDV CRYFTDIIKC RVINTSHLSI
360 370 380 390 400
HKTWEEARLH GAWTLHEDPR QNRGGGCINH KDTFFQNPQY IFEVKKPEDE
410 420 430 440 450
VLICIQQRPK RSTRREGKGE NLAIGFDIYK VEENRQYRMH SLQHKAASSI
460 470 480 490 500
YINSRSVFLR TDQPEGRYVI IPTTFEPGHT GEFLLRVFTD VPSNCRELRL
510 520 530 540 550
DEPPHTCWSS LCGYPQLVTQ VHVLGAAGLK DSPTGANSYV IIKCEGDKVR
560 570 580 590 600
SAVQKGTSTP EYNVKGIFYR KKLSQPITVQ VWNHRVLKDE FLGQVHLKAD
610 620 630 640
PDNLQALHTL HLRDRNSRQP SNLPGTVAVH ILSSTSLMAV
Length:640
Mass (Da):73,169
Last modified:February 12, 2003 - v2
Checksum:i7A3A9A1A920410BC
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E7EV01E7EV01_HUMAN
Calpain-5
CAPN5
680Annotation score:
E9PS73E9PS73_HUMAN
Calpain-5
CAPN5
97Annotation score:
K7EP62K7EP62_HUMAN
Calpain-5
CAPN5
52Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18R → Q in AAC51869 (PubMed:9367857).Curated1
Sequence conflicti51W → R in CAA71584 (PubMed:9339374).Curated1
Sequence conflicti112 – 115EKPN → RKAQ in AAC51869 (PubMed:9367857).Curated4
Sequence conflicti128 – 131FGEW → LGM in AAC51869 (PubMed:9367857).Curated4
Sequence conflicti138D → E in AAC51869 (PubMed:9367857).Curated1
Sequence conflicti502E → K in AAC51869 (PubMed:9367857).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_069277243R → L in VRNI; largely mislocalized to the cytoplasm whereas the wild-type protein is localized near the cell surface. 1 PublicationCorresponds to variant dbSNP:rs397514601EnsemblClinVar.1
Natural variantiVAR_069278244L → P in VRNI; largely mislocalized to the cytoplasm whereas the wild-type protein is localized near the cell surface. 1 PublicationCorresponds to variant dbSNP:rs397514602EnsemblClinVar.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94346 mRNA Translation: AAC51869.1
BC018123 mRNA Translation: AAH18123.1
Y10552 mRNA Translation: CAA71584.1
CCDSiCCDS8248.1
PIRiJC5772
RefSeqiNP_004046.2, NM_004055.4
XP_016873712.1, XM_017018223.1
UniGeneiHs.248153

Genome annotation databases

EnsembliENST00000278559; ENSP00000278559; ENSG00000149260
ENST00000529629; ENSP00000432332; ENSG00000149260
GeneIDi726
KEGGihsa:726
UCSCiuc001oxx.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U94346 mRNA Translation: AAC51869.1
BC018123 mRNA Translation: AAH18123.1
Y10552 mRNA Translation: CAA71584.1
CCDSiCCDS8248.1
PIRiJC5772
RefSeqiNP_004046.2, NM_004055.4
XP_016873712.1, XM_017018223.1
UniGeneiHs.248153

3D structure databases

ProteinModelPortaliO15484
SMRiO15484
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107187, 3 interactors
IntActiO15484, 2 interactors
STRINGi9606.ENSP00000278559

Protein family/group databases

MEROPSiC02.011

PTM databases

iPTMnetiO15484
PhosphoSitePlusiO15484
SwissPalmiO15484

Polymorphism and mutation databases

BioMutaiCAPN5

Proteomic databases

EPDiO15484
MaxQBiO15484
PaxDbiO15484
PeptideAtlasiO15484
PRIDEiO15484
ProteomicsDBi48688

Protocols and materials databases

DNASUi726
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000278559; ENSP00000278559; ENSG00000149260
ENST00000529629; ENSP00000432332; ENSG00000149260
GeneIDi726
KEGGihsa:726
UCSCiuc001oxx.4 human

Organism-specific databases

CTDi726
DisGeNETi726
EuPathDBiHostDB:ENSG00000149260.14
GeneCardsiCAPN5
HGNCiHGNC:1482 CAPN5
HPAiCAB033237
MalaCardsiCAPN5
MIMi193235 phenotype
602537 gene
neXtProtiNX_O15484
OpenTargetsiENSG00000149260
Orphaneti329211 Autosomal dominant neovascular inflammatory vitreoretinopathy
PharmGKBiPA26062
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
GeneTreeiENSGT00760000118971
HOGENOMiHOG000232036
HOVERGENiHBG001095
InParanoidiO15484
KOiK08574
PhylomeDBiO15484
TreeFamiTF314748

Enzyme and pathway databases

BRENDAi3.4.22.B25 2681
ReactomeiR-HSA-1474228 Degradation of the extracellular matrix

Miscellaneous databases

ChiTaRSiCAPN5 human
GeneWikiiCAPN5
GenomeRNAii726
PROiPR:O15484
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000149260 Expressed in 171 organ(s), highest expression level in transverse colon
CleanExiHS_CAPN5
ExpressionAtlasiO15484 baseline and differential
GenevisibleiO15484 HS

Family and domain databases

CDDicd04046 C2_Calpain, 1 hit
cd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR033884 C2_Calpain
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SUPFAMiSSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00139 THIOL_PROTEASE_CYS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCAN5_HUMAN
AccessioniPrimary (citable) accession number: O15484
Secondary accession number(s): O00263
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: February 12, 2003
Last modified: November 7, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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