UniProtKB - O15444 (CCL25_HUMAN)
Protein
C-C motif chemokine 25
Gene
CCL25
Organism
Homo sapiens (Human)
Status
Functioni
Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
GO - Molecular functioni
- CCR10 chemokine receptor binding Source: BHF-UCL
- CCR chemokine receptor binding Source: GO_Central
- chemokine activity Source: UniProtKB
- chemokine receptor binding Source: UniProtKB
- hormone activity Source: ProtInc
GO - Biological processi
- antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
- cell chemotaxis Source: UniProtKB
- cell surface receptor signaling pathway Source: ProtInc
- cellular response to interferon-gamma Source: GO_Central
- cellular response to interleukin-1 Source: GO_Central
- cellular response to tumor necrosis factor Source: GO_Central
- chemokine-mediated signaling pathway Source: GO_Central
- chemotaxis Source: ProtInc
- G protein-coupled receptor signaling pathway Source: UniProtKB
- immune response Source: ProtInc
- inflammatory response Source: GO_Central
- killing of cells of other organism Source: UniProtKB
- lymphocyte chemotaxis Source: GO_Central
- monocyte chemotaxis Source: GO_Central
- negative regulation of leukocyte tethering or rolling Source: UniProtKB
- neutrophil chemotaxis Source: GO_Central
- positive regulation of cell-matrix adhesion Source: UniProtKB
- positive regulation of ERK1 and ERK2 cascade Source: GO_Central
- positive regulation of GTPase activity Source: GO_Central
Keywordsi
Molecular function | Cytokine |
Biological process | Chemotaxis, Inflammatory response |
Enzyme and pathway databases
PathwayCommonsi | O15444 |
Reactomei | R-HSA-380108, Chemokine receptors bind chemokines R-HSA-418594, G alpha (i) signalling events |
SIGNORi | O15444 |
Names & Taxonomyi
Protein namesi | Recommended name: C-C motif chemokine 25Alternative name(s): Chemokine TECK Small-inducible cytokine A25 Thymus-expressed chemokine |
Gene namesi | Name:CCL25 Synonyms:SCYA25, TECK |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10624, CCL25 |
MIMi | 602565, gene |
neXtProti | NX_O15444 |
VEuPathDBi | HostDB:ENSG00000131142.13 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: GO_Central
Keywords - Cellular componenti
SecretedPathology & Biotechi
Organism-specific databases
DisGeNETi | 6370 |
OpenTargetsi | ENSG00000131142 |
PharmGKBi | PA35556 |
Miscellaneous databases
Pharosi | O15444, Tbio |
Genetic variation databases
BioMutai | CCL25 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000005235 | 24 – 150 | C-C motif chemokine 25Add BLAST | 127 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 30 ↔ 58 | By similarity | ||
Disulfide bondi | 31 ↔ 75 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | O15444 |
PeptideAtlasi | O15444 |
PRIDEi | O15444 |
ProteomicsDBi | 48672 [O15444-1] 48673 [O15444-2] 48674 [O15444-3] |
PTM databases
iPTMneti | O15444 |
PhosphoSitePlusi | O15444 |
Expressioni
Tissue specificityi
Specifically expressed by thymic dendritic cells. High levels in thymus and small intestine.
Gene expression databases
Bgeei | ENSG00000131142, Expressed in small intestine and 91 other tissues |
ExpressionAtlasi | O15444, baseline and differential |
Genevisiblei | O15444, HS |
Organism-specific databases
HPAi | ENSG00000131142, Group enriched (intestine, lymphoid tissue) |
Interactioni
Binary interactionsi
Hide detailsO15444
GO - Molecular functioni
- CCR10 chemokine receptor binding Source: BHF-UCL
- CCR chemokine receptor binding Source: GO_Central
- chemokine activity Source: UniProtKB
- chemokine receptor binding Source: UniProtKB
- hormone activity Source: ProtInc
Protein-protein interaction databases
BioGRIDi | 112273, 3 interactors |
DIPi | DIP-5883N |
IntActi | O15444, 11 interactors |
MINTi | O15444 |
STRINGi | 9606.ENSP00000375086 |
Miscellaneous databases
RNActi | O15444, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the intercrine beta (chemokine CC) family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | ENOG502S8D1, Eukaryota |
GeneTreei | ENSGT00970000193327 |
HOGENOMi | CLU_113773_0_0_1 |
InParanoidi | O15444 |
OMAi | CLAYHHR |
OrthoDBi | 1542802at2759 |
PhylomeDBi | O15444 |
TreeFami | TF353160 |
Family and domain databases
CDDi | cd01119, Chemokine_CC_DCCL, 1 hit |
InterProi | View protein in InterPro IPR039809, Chemokine_b/g/d IPR034133, Chemokine_CC_DCCL IPR001811, Chemokine_IL8-like_dom IPR036048, Interleukin_8-like_sf |
PANTHERi | PTHR12015, PTHR12015, 1 hit |
Pfami | View protein in Pfam PF00048, IL8, 1 hit |
SMARTi | View protein in SMART SM00199, SCY, 1 hit |
SUPFAMi | SSF54117, SSF54117, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O15444-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MNLWLLACLV AGFLGAWAPA VHTQGVFEDC CLAYHYPIGW AVLRRAWTYR
60 70 80 90 100
IQEVSGSCNL PAAIFYLPKR HRKVCGNPKS REVQRAMKLL DARNKVFAKL
110 120 130 140 150
HHNTQTFQAG PHAVKKLSSG NSKLSSSKFS NPISSSKRNV SLLISANSGL
Isoform 2 (identifier: O15444-2) [UniParc]FASTAAdd to basket
Also known as: TECKvar
The sequence of this isoform differs from the canonical sequence as follows:
65-150: FYLPKRHRKV...SLLISANSGL → RPSCCKEVEFWKLQVIIIQV
Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.Curated
Show »Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JDZ7 | C9JDZ7_HUMAN | C-C motif chemokine 25 | CCL25 | 150 | Annotation score: | ||
F8VWI0 | F8VWI0_HUMAN | C-C motif chemokine 25 | CCL25 | 84 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_044519 | 23 | T → A. Corresponds to variant dbSNP:rs960173Ensembl. | 1 | |
Natural variantiVAR_044520 | 101 | H → R. Corresponds to variant dbSNP:rs2032887Ensembl. | 1 | |
Natural variantiVAR_044521 | 104 | T → M1 PublicationCorresponds to variant dbSNP:rs1129763Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001064 | 65 – 150 | FYLPK…ANSGL → RPSCCKEVEFWKLQVIIIQV in isoform 2. 1 PublicationAdd BLAST | 86 | |
Alternative sequenceiVSP_043199 | 109 | Missing in isoform 3. 2 Publications | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U86358 mRNA Translation: AAB69981.1 AB046579 mRNA Translation: BAB62257.1 AK292662 mRNA Translation: BAF85351.1 AC008946 Genomic DNA No translation available. CH471139 Genomic DNA Translation: EAW68952.1 BC130561 mRNA Translation: AAI30562.1 BC144463 mRNA Translation: AAI44464.1 |
CCDSi | CCDS12194.1 [O15444-1] CCDS56080.1 [O15444-3] |
RefSeqi | NP_001188288.1, NM_001201359.1 [O15444-3] NP_005615.2, NM_005624.3 [O15444-1] XP_011526479.1, XM_011528177.2 [O15444-1] XP_016882609.1, XM_017027120.1 [O15444-1] XP_016882610.1, XM_017027121.1 [O15444-1] XP_016882611.1, XM_017027122.1 [O15444-3] |
Genome annotation databases
Ensembli | ENST00000253451; ENSP00000253451; ENSG00000131142 [O15444-3] ENST00000390669; ENSP00000375086; ENSG00000131142 [O15444-1] |
GeneIDi | 6370 |
KEGGi | hsa:6370 |
UCSCi | uc002mjc.5, human [O15444-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Wikipedia CCL25 entry |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U86358 mRNA Translation: AAB69981.1 AB046579 mRNA Translation: BAB62257.1 AK292662 mRNA Translation: BAF85351.1 AC008946 Genomic DNA No translation available. CH471139 Genomic DNA Translation: EAW68952.1 BC130561 mRNA Translation: AAI30562.1 BC144463 mRNA Translation: AAI44464.1 |
CCDSi | CCDS12194.1 [O15444-1] CCDS56080.1 [O15444-3] |
RefSeqi | NP_001188288.1, NM_001201359.1 [O15444-3] NP_005615.2, NM_005624.3 [O15444-1] XP_011526479.1, XM_011528177.2 [O15444-1] XP_016882609.1, XM_017027120.1 [O15444-1] XP_016882610.1, XM_017027121.1 [O15444-1] XP_016882611.1, XM_017027122.1 [O15444-3] |
3D structure databases
SMRi | O15444 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 112273, 3 interactors |
DIPi | DIP-5883N |
IntActi | O15444, 11 interactors |
MINTi | O15444 |
STRINGi | 9606.ENSP00000375086 |
PTM databases
iPTMneti | O15444 |
PhosphoSitePlusi | O15444 |
Genetic variation databases
BioMutai | CCL25 |
Proteomic databases
PaxDbi | O15444 |
PeptideAtlasi | O15444 |
PRIDEi | O15444 |
ProteomicsDBi | 48672 [O15444-1] 48673 [O15444-2] 48674 [O15444-3] |
Protocols and materials databases
Antibodypediai | 24738, 393 antibodies |
DNASUi | 6370 |
Genome annotation databases
Ensembli | ENST00000253451; ENSP00000253451; ENSG00000131142 [O15444-3] ENST00000390669; ENSP00000375086; ENSG00000131142 [O15444-1] |
GeneIDi | 6370 |
KEGGi | hsa:6370 |
UCSCi | uc002mjc.5, human [O15444-1] |
Organism-specific databases
CTDi | 6370 |
DisGeNETi | 6370 |
GeneCardsi | CCL25 |
HGNCi | HGNC:10624, CCL25 |
HPAi | ENSG00000131142, Group enriched (intestine, lymphoid tissue) |
MIMi | 602565, gene |
neXtProti | NX_O15444 |
OpenTargetsi | ENSG00000131142 |
PharmGKBi | PA35556 |
VEuPathDBi | HostDB:ENSG00000131142.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502S8D1, Eukaryota |
GeneTreei | ENSGT00970000193327 |
HOGENOMi | CLU_113773_0_0_1 |
InParanoidi | O15444 |
OMAi | CLAYHHR |
OrthoDBi | 1542802at2759 |
PhylomeDBi | O15444 |
TreeFami | TF353160 |
Enzyme and pathway databases
PathwayCommonsi | O15444 |
Reactomei | R-HSA-380108, Chemokine receptors bind chemokines R-HSA-418594, G alpha (i) signalling events |
SIGNORi | O15444 |
Miscellaneous databases
BioGRID-ORCSi | 6370, 7 hits in 875 CRISPR screens |
GenomeRNAii | 6370 |
Pharosi | O15444, Tbio |
PROi | PR:O15444 |
RNActi | O15444, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000131142, Expressed in small intestine and 91 other tissues |
ExpressionAtlasi | O15444, baseline and differential |
Genevisiblei | O15444, HS |
Family and domain databases
CDDi | cd01119, Chemokine_CC_DCCL, 1 hit |
InterProi | View protein in InterPro IPR039809, Chemokine_b/g/d IPR034133, Chemokine_CC_DCCL IPR001811, Chemokine_IL8-like_dom IPR036048, Interleukin_8-like_sf |
PANTHERi | PTHR12015, PTHR12015, 1 hit |
Pfami | View protein in Pfam PF00048, IL8, 1 hit |
SMARTi | View protein in SMART SM00199, SCY, 1 hit |
SUPFAMi | SSF54117, SSF54117, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CCL25_HUMAN | |
Accessioni | O15444Primary (citable) accession number: O15444 Secondary accession number(s): A1L4J4 Q96KJ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 22, 2008 | |
Last modified: | February 10, 2021 | |
This is version 160 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants