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Protein

Eukaryotic translation initiation factor 3 subunit H

Gene

EIF3H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).UniRule annotation3 Publications

GO - Molecular functioni

  • metallopeptidase activity Source: GO_Central
  • RNA binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB-UniRule

GO - Biological processi

  • formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
  • regulation of translational initiation Source: UniProtKB
  • translational initiation Source: UniProtKB

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72649 Translation initiation complex formation
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Protein family/group databases

MEROPSiM67.971

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit HUniRule annotation
Short name:
eIF3hUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 3UniRule annotation
eIF-3-gamma
eIF3 p40 subunitUniRule annotation
Gene namesi
Name:EIF3HUniRule annotation
Synonyms:EIF3S3UniRule annotation
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000147677.10
HGNCiHGNC:3273 EIF3H
MIMi603912 gene
neXtProtiNX_O15372

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi8667
OpenTargetsiENSG00000147677
PharmGKBiPA162384854

Polymorphism and mutation databases

BioMutaiEIF3H

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139611 – 352Eukaryotic translation initiation factor 3 subunit HAdd BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineCombined sources1
Modified residuei183PhosphoserineUniRule annotationCombined sources1 Publication1
Cross-linki303Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO15372
PaxDbiO15372
PeptideAtlasiO15372
PRIDEiO15372
ProteomicsDBi48615

PTM databases

iPTMnetiO15372
PhosphoSitePlusiO15372
SwissPalmiO15372

Expressioni

Gene expression databases

BgeeiENSG00000147677 Expressed in 234 organ(s), highest expression level in female gonad
CleanExiHS_EIF3H
ExpressionAtlasiO15372 baseline and differential
GenevisibleiO15372 HS

Organism-specific databases

HPAiHPA023117
HPA023553

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DHX33; the interaction is independent of RNA (PubMed:26100019).UniRule annotation7 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi114216, 118 interactors
CORUMiO15372
DIPiDIP-33689N
IntActiO15372, 64 interactors
MINTiO15372
STRINGi9606.ENSP00000429931

Structurei

Secondary structure

1352
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO15372
SMRiO15372
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 173MPNPROSITE-ProRule annotationAdd BLAST135

Sequence similaritiesi

Belongs to the eIF-3 subunit H family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1560 Eukaryota
ENOG410XSR9 LUCA
GeneTreeiENSGT00730000111042
HOVERGENiHBG000883
InParanoidiO15372
KOiK03247
PhylomeDBiO15372
TreeFamiTF101504

Family and domain databases

CDDicd08065 MPN_eIF3h, 1 hit
HAMAPiMF_03007 eIF3h, 1 hit
InterProiView protein in InterPro
IPR027524 eIF3h
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
PANTHERiPTHR10410:SF3 PTHR10410:SF3, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All

O15372-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII
60 70 80 90 100
KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTED DADFDEVQYQ
110 120 130 140 150
MEMMRSLRHV NIDHLHVGWY QSTYYGSFVT RALLDSQFSY QHAIEESVVL
160 170 180 190 200
IYDPIKTAQG SLSLKAYRLT PKLMEVCKEK DFSPEALKKA NITFEYMFEE
210 220 230 240 250
VPIVIKNSHL INVLMWELEK KSAVADKHEL LSLASSNHLG KNLQLLMDRV
260 270 280 290 300
DEMSQDIVKY NTYMRNTSKQ QQQKHQYQQR RQQENMQRQS RGEPPLPEED
310 320 330 340 350
LSKLFKPPQP PARMDSLLIA GQINTYCQNI KEFTAQNLGK LFMAQALQEY

NN
Length:352
Mass (Da):39,930
Last modified:January 1, 1998 - v1
Checksum:iF3A6EFA0CEF587D0
GO

Computationally mapped potential isoform sequencesi

There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B3KS98B3KS98_HUMAN
Eukaryotic translation initiation f...
EIF3H EIF3S3, hCG_20900
366Annotation score:
A0A087WZK9A0A087WZK9_HUMAN
Eukaryotic translation initiation f...
EIF3H EIF3S3
349Annotation score:
E5RJT0E5RJT0_HUMAN
Eukaryotic translation initiation f...
EIF3H
244Annotation score:
E5RGU4E5RGU4_HUMAN
Eukaryotic translation initiation f...
EIF3H
235Annotation score:
E5RFH0E5RFH0_HUMAN
Eukaryotic translation initiation f...
EIF3H
170Annotation score:
E5RFW7E5RFW7_HUMAN
Eukaryotic translation initiation f...
EIF3H
97Annotation score:
E5RH59E5RH59_HUMAN
Eukaryotic translation initiation f...
EIF3H
56Annotation score:
E5RHC7E5RHC7_HUMAN
Eukaryotic translation initiation f...
EIF3H
46Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73E → K in AAC84044 (Ref. 2) Curated1

Mass spectrometryi

Molecular mass is 40010.4 Da from positions 1 - 352. 1 Publication
Molecular mass is 39842.6±0.4 Da from positions 1 - 352. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54559 mRNA Translation: AAD03465.1
AF092576
, AF092569, AF092570, AF092571, AF092572, AF092573, AF092574, AF092575 Genomic DNA Translation: AAC84044.1
BC000386 mRNA Translation: AAH00386.1
CCDSiCCDS6319.1
RefSeqiNP_003747.1, NM_003756.2
UniGeneiHs.492599

Genome annotation databases

EnsembliENST00000521861; ENSP00000429931; ENSG00000147677
GeneIDi8667
KEGGihsa:8667

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U54559 mRNA Translation: AAD03465.1
AF092576
, AF092569, AF092570, AF092571, AF092572, AF092573, AF092574, AF092575 Genomic DNA Translation: AAC84044.1
BC000386 mRNA Translation: AAH00386.1
CCDSiCCDS6319.1
RefSeqiNP_003747.1, NM_003756.2
UniGeneiHs.492599

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J8Belectron microscopy-H1-221[»]
3J8Celectron microscopy-H1-221[»]
ProteinModelPortaliO15372
SMRiO15372
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114216, 118 interactors
CORUMiO15372
DIPiDIP-33689N
IntActiO15372, 64 interactors
MINTiO15372
STRINGi9606.ENSP00000429931

Protein family/group databases

MEROPSiM67.971

PTM databases

iPTMnetiO15372
PhosphoSitePlusiO15372
SwissPalmiO15372

Polymorphism and mutation databases

BioMutaiEIF3H

Proteomic databases

EPDiO15372
PaxDbiO15372
PeptideAtlasiO15372
PRIDEiO15372
ProteomicsDBi48615

Protocols and materials databases

DNASUi8667
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000521861; ENSP00000429931; ENSG00000147677
GeneIDi8667
KEGGihsa:8667

Organism-specific databases

CTDi8667
DisGeNETi8667
EuPathDBiHostDB:ENSG00000147677.10
GeneCardsiEIF3H
HGNCiHGNC:3273 EIF3H
HPAiHPA023117
HPA023553
MIMi603912 gene
neXtProtiNX_O15372
OpenTargetsiENSG00000147677
PharmGKBiPA162384854
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1560 Eukaryota
ENOG410XSR9 LUCA
GeneTreeiENSGT00730000111042
HOVERGENiHBG000883
InParanoidiO15372
KOiK03247
PhylomeDBiO15372
TreeFamiTF101504

Enzyme and pathway databases

ReactomeiR-HSA-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-HSA-72649 Translation initiation complex formation
R-HSA-72689 Formation of a pool of free 40S subunits
R-HSA-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-HSA-72702 Ribosomal scanning and start codon recognition
R-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Miscellaneous databases

ChiTaRSiEIF3H human
GeneWikiiEIF3H
GenomeRNAii8667
PROiPR:O15372
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147677 Expressed in 234 organ(s), highest expression level in female gonad
CleanExiHS_EIF3H
ExpressionAtlasiO15372 baseline and differential
GenevisibleiO15372 HS

Family and domain databases

CDDicd08065 MPN_eIF3h, 1 hit
HAMAPiMF_03007 eIF3h, 1 hit
InterProiView protein in InterPro
IPR027524 eIF3h
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
PANTHERiPTHR10410:SF3 PTHR10410:SF3, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3H_HUMAN
AccessioniPrimary (citable) accession number: O15372
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 172 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
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