UniProtKB - O15372 (EIF3H_HUMAN)
Eukaryotic translation initiation factor 3 subunit H
EIF3H
Functioni
GO - Molecular functioni
- isopeptidase activity Source: InterPro
- metallopeptidase activity Source: GO_Central
- RNA binding Source: UniProtKB
- translation initiation factor activity Source: UniProtKB-UniRule
- ubiquitinyl hydrolase activity Source: FlyBase
GO - Biological processi
- formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
- negative regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
- regulation of translational initiation Source: UniProtKB
- translational initiation Source: UniProtKB
Keywordsi
Molecular function | Initiation factor |
Biological process | Protein biosynthesis |
Enzyme and pathway databases
PathwayCommonsi | O15372 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-72649, Translation initiation complex formation R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72695, Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72702, Ribosomal scanning and start codon recognition R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Protein family/group databases
MEROPSi | M67.971 |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 3 subunit HUniRule annotationShort name: eIF3hUniRule annotation Alternative name(s): Eukaryotic translation initiation factor 3 subunit 3UniRule annotation eIF-3-gamma eIF3 p40 subunitUniRule annotation |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3273, EIF3H |
MIMi | 603912, gene |
neXtProti | NX_O15372 |
VEuPathDBi | HostDB:ENSG00000147677.10 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Cytosol
- cytosol Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Other locations
- eukaryotic 43S preinitiation complex Source: GO_Central
- eukaryotic 48S preinitiation complex Source: UniProtKB-UniRule
- eukaryotic translation initiation factor 3 complex Source: UniProtKB
- membrane Source: UniProtKB
- polysomal ribosome Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 8667 |
OpenTargetsi | ENSG00000147677 |
PharmGKBi | PA162384854 |
Miscellaneous databases
Pharosi | O15372, Tbio |
Genetic variation databases
BioMutai | EIF3H |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000213961 | 1 – 352 | Eukaryotic translation initiation factor 3 subunit HAdd BLAST | 352 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 3 | PhosphoserineCombined sources | 1 | |
Modified residuei | 183 | PhosphoserineUniRule annotationCombined sources1 Publication | 1 | |
Cross-linki | 303 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O15372 |
jPOSTi | O15372 |
MassIVEi | O15372 |
PaxDbi | O15372 |
PeptideAtlasi | O15372 |
PRIDEi | O15372 |
ProteomicsDBi | 48615 |
PTM databases
iPTMneti | O15372 |
MetOSitei | O15372 |
PhosphoSitePlusi | O15372 |
SwissPalmi | O15372 |
Expressioni
Gene expression databases
Bgeei | ENSG00000147677, Expressed in female gonad and 247 other tissues |
ExpressionAtlasi | O15372, baseline and differential |
Genevisiblei | O15372, HS |
Organism-specific databases
HPAi | ENSG00000147677, Low tissue specificity |
Interactioni
Subunit structurei
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner.
Interacts with DHX33; the interaction is independent of RNA (PubMed:26100019).
UniRule annotation7 PublicationsBinary interactionsi
Hide detailsO15372
Protein-protein interaction databases
BioGRIDi | 114216, 153 interactors |
ComplexPortali | CPX-6036, Eukaryotic translation initiation factor 3 complex |
CORUMi | O15372 |
DIPi | DIP-33689N |
IntActi | O15372, 84 interactors |
MINTi | O15372 |
STRINGi | 9606.ENSP00000429931 |
Miscellaneous databases
RNActi | O15372, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O15372 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 39 – 173 | MPNPROSITE-ProRule annotationAdd BLAST | 135 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1560, Eukaryota |
GeneTreei | ENSGT00730000111042 |
HOGENOMi | CLU_044094_0_0_1 |
InParanoidi | O15372 |
OrthoDBi | 976469at2759 |
PhylomeDBi | O15372 |
TreeFami | TF101504 |
Family and domain databases
CDDi | cd08065, MPN_eIF3h, 1 hit |
HAMAPi | MF_03007, eIF3h, 1 hit |
InterProi | View protein in InterPro IPR027524, eIF3h IPR000555, JAMM/MPN+_dom IPR037518, MPN |
PANTHERi | PTHR10410:SF3, PTHR10410:SF3, 1 hit |
Pfami | View protein in Pfam PF01398, JAB, 1 hit |
SMARTi | View protein in SMART SM00232, JAB_MPN, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII
60 70 80 90 100
KHYQEEGQGT EVVQGVLLGL VVEDRLEITN CFPFPQHTED DADFDEVQYQ
110 120 130 140 150
MEMMRSLRHV NIDHLHVGWY QSTYYGSFVT RALLDSQFSY QHAIEESVVL
160 170 180 190 200
IYDPIKTAQG SLSLKAYRLT PKLMEVCKEK DFSPEALKKA NITFEYMFEE
210 220 230 240 250
VPIVIKNSHL INVLMWELEK KSAVADKHEL LSLASSNHLG KNLQLLMDRV
260 270 280 290 300
DEMSQDIVKY NTYMRNTSKQ QQQKHQYQQR RQQENMQRQS RGEPPLPEED
310 320 330 340 350
LSKLFKPPQP PARMDSLLIA GQINTYCQNI KEFTAQNLGK LFMAQALQEY
NN
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB3KS98 | B3KS98_HUMAN | Eukaryotic translation initiation f... | EIF3H EIF3S3, hCG_20900 | 366 | Annotation score: | ||
A0A087WZK9 | A0A087WZK9_HUMAN | Eukaryotic translation initiation f... | EIF3H EIF3S3 | 349 | Annotation score: | ||
E5RGU4 | E5RGU4_HUMAN | Eukaryotic translation initiation f... | EIF3H | 235 | Annotation score: | ||
E5RFH0 | E5RFH0_HUMAN | Eukaryotic translation initiation f... | EIF3H | 170 | Annotation score: | ||
E5RFW7 | E5RFW7_HUMAN | Eukaryotic translation initiation f... | EIF3H | 97 | Annotation score: | ||
E5RJT0 | E5RJT0_HUMAN | Eukaryotic translation initiation f... | EIF3H | 244 | Annotation score: | ||
E5RH59 | E5RH59_HUMAN | Eukaryotic translation initiation f... | EIF3H | 56 | Annotation score: | ||
E5RHC7 | E5RHC7_HUMAN | Eukaryotic translation initiation f... | EIF3H | 46 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 73 | E → K in AAC84044 (Ref. 2) Curated | 1 |
Mass spectrometryi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54559 mRNA Translation: AAD03465.1 AF092576 , AF092569, AF092570, AF092571, AF092572, AF092573, AF092574, AF092575 Genomic DNA Translation: AAC84044.1 BC000386 mRNA Translation: AAH00386.1 |
CCDSi | CCDS6319.1 |
RefSeqi | NP_003747.1, NM_003756.2 |
Genome annotation databases
Ensembli | ENST00000521861; ENSP00000429931; ENSG00000147677 |
GeneIDi | 8667 |
KEGGi | hsa:8667 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54559 mRNA Translation: AAD03465.1 AF092576 , AF092569, AF092570, AF092571, AF092572, AF092573, AF092574, AF092575 Genomic DNA Translation: AAC84044.1 BC000386 mRNA Translation: AAH00386.1 |
CCDSi | CCDS6319.1 |
RefSeqi | NP_003747.1, NM_003756.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3J8B | electron microscopy | - | H | 1-221 | [»] | |
3J8C | electron microscopy | - | H | 1-221 | [»] | |
6YBD | electron microscopy | 3.30 | 8 | 1-352 | [»] | |
6ZMW | electron microscopy | 3.70 | 8 | 1-352 | [»] | |
6ZON | electron microscopy | 3.00 | H | 1-352 | [»] | |
6ZP4 | electron microscopy | 2.90 | H | 1-352 | [»] | |
6ZVJ | electron microscopy | 3.80 | H | 35-352 | [»] | |
7A09 | electron microscopy | 3.50 | H | 1-352 | [»] | |
SMRi | O15372 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114216, 153 interactors |
ComplexPortali | CPX-6036, Eukaryotic translation initiation factor 3 complex |
CORUMi | O15372 |
DIPi | DIP-33689N |
IntActi | O15372, 84 interactors |
MINTi | O15372 |
STRINGi | 9606.ENSP00000429931 |
Protein family/group databases
MEROPSi | M67.971 |
PTM databases
iPTMneti | O15372 |
MetOSitei | O15372 |
PhosphoSitePlusi | O15372 |
SwissPalmi | O15372 |
Genetic variation databases
BioMutai | EIF3H |
Proteomic databases
EPDi | O15372 |
jPOSTi | O15372 |
MassIVEi | O15372 |
PaxDbi | O15372 |
PeptideAtlasi | O15372 |
PRIDEi | O15372 |
ProteomicsDBi | 48615 |
Protocols and materials databases
Antibodypediai | 13577, 291 antibodies |
DNASUi | 8667 |
Genome annotation databases
Ensembli | ENST00000521861; ENSP00000429931; ENSG00000147677 |
GeneIDi | 8667 |
KEGGi | hsa:8667 |
Organism-specific databases
CTDi | 8667 |
DisGeNETi | 8667 |
GeneCardsi | EIF3H |
HGNCi | HGNC:3273, EIF3H |
HPAi | ENSG00000147677, Low tissue specificity |
MIMi | 603912, gene |
neXtProti | NX_O15372 |
OpenTargetsi | ENSG00000147677 |
PharmGKBi | PA162384854 |
VEuPathDBi | HostDB:ENSG00000147677.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1560, Eukaryota |
GeneTreei | ENSGT00730000111042 |
HOGENOMi | CLU_044094_0_0_1 |
InParanoidi | O15372 |
OrthoDBi | 976469at2759 |
PhylomeDBi | O15372 |
TreeFami | TF101504 |
Enzyme and pathway databases
PathwayCommonsi | O15372 |
Reactomei | R-HSA-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-HSA-72649, Translation initiation complex formation R-HSA-72689, Formation of a pool of free 40S subunits R-HSA-72695, Formation of the ternary complex, and subsequently, the 43S complex R-HSA-72702, Ribosomal scanning and start codon recognition R-HSA-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Miscellaneous databases
BioGRID-ORCSi | 8667, 508 hits in 1016 CRISPR screens |
ChiTaRSi | EIF3H, human |
GeneWikii | EIF3H |
GenomeRNAii | 8667 |
Pharosi | O15372, Tbio |
PROi | PR:O15372 |
RNActi | O15372, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000147677, Expressed in female gonad and 247 other tissues |
ExpressionAtlasi | O15372, baseline and differential |
Genevisiblei | O15372, HS |
Family and domain databases
CDDi | cd08065, MPN_eIF3h, 1 hit |
HAMAPi | MF_03007, eIF3h, 1 hit |
InterProi | View protein in InterPro IPR027524, eIF3h IPR000555, JAMM/MPN+_dom IPR037518, MPN |
PANTHERi | PTHR10410:SF3, PTHR10410:SF3, 1 hit |
Pfami | View protein in Pfam PF01398, JAB, 1 hit |
SMARTi | View protein in SMART SM00232, JAB_MPN, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EIF3H_HUMAN | |
Accessioni | O15372Primary (citable) accession number: O15372 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 191 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Translation initiation factors
List of translation initiation factor entries - Human chromosome 8
Human chromosome 8: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families