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Protein

DNA-directed RNA polymerase III subunit RPC7

Gene

POLR3G

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs (PubMed:20154270). May direct with other members of the RPC3/POLR3C-RPC6/POLR3F-RPC7/POLR3G subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs), induce type I interferon and NF- Kappa-B through the RIG-I pathway (PubMed:19609254, PubMed:19631370).3 Publications

GO - Molecular functioni

  • DNA-directed 5'-3' RNA polymerase activity Source: ProtInc

GO - Biological processi

  • cell proliferation Source: MGI
  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • positive regulation of innate immune response Source: UniProtKB
  • positive regulation of interferon-beta production Source: UniProtKB
  • regulation of transcription by RNA polymerase III Source: ProtInc
  • transcription by RNA polymerase III Source: UniProtKB

Keywordsi

Biological processAntiviral defense, Immunity, Innate immunity, Transcription

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase III subunit RPC7
Short name:
RNA polymerase III subunit C7
Alternative name(s):
DNA-directed RNA polymerase III subunit G
RNA polymerase III 32 kDa apha subunit
Short name:
RPC32-alpha1 Publication
RNA polymerase III 32 kDa subunit
Short name:
RPC32
Gene namesi
Name:POLR3G
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000113356.10
HGNCiHGNC:30075 POLR3G
MIMi617456 gene
neXtProtiNX_O15318

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000113356
PharmGKBiPA134986024

Polymorphism and mutation databases

BioMutaiPOLR3G

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000739781 – 223DNA-directed RNA polymerase III subunit RPC7Add BLAST223

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei133PhosphothreonineCombined sources1
Modified residuei157PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO15318
MaxQBiO15318
PaxDbiO15318
PeptideAtlasiO15318
PRIDEiO15318
ProteomicsDBi48580

PTM databases

iPTMnetiO15318
PhosphoSitePlusiO15318

Expressioni

Tissue specificityi

Barely detectable in differentiated tissues. Expressed in embryonic stem cells and in other dividing cells, such as some tumor cell lines.3 Publications

Developmental stagei

Down-regulated in embryonic stem cells upon differentiation into embroid bodies (at protein level) (PubMed:20154270, PubMed:21898682, PubMed:28494942). An analogous down-regulation is observed during differentiation of induced pluripotent stem cells (PubMed:21898682).3 Publications

Inductioni

Induced by NANOG and POU5F1/OCT4 (PubMed:21898682). Negatively regulated by the interaction of microRNA MIR1305 with 3 miRNA responsive elements (miREs) in its 3'-UTR (PubMed:27339422).2 Publications

Gene expression databases

BgeeiENSG00000113356 Expressed in 178 organ(s), highest expression level in dorsal root ganglion
CleanExiHS_POLR3G
ExpressionAtlasiO15318 baseline and differential
GenevisibleiO15318 HS

Organism-specific databases

HPAiCAB010109
HPA052658

Interactioni

Subunit structurei

Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits (By similarity). RPC3/POLR3C, RPC6/POLR3F and RPC7/POLR3G form a Pol III subcomplex. Directly interacts with POLR3C/RPC62 (PubMed:21358628, PubMed:24107381, PubMed:26394183). Also found a trimeric complex with POLR3C and POLR3GL (PubMed:24107381).By similarity4 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi115867, 15 interactors
DIPiDIP-59079N
IntActiO15318, 3 interactors
STRINGi9606.ENSP00000382058

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi147 – 196Glu-richAdd BLAST50
Compositional biasi175 – 181Poly-Asp7

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IKHU Eukaryota
ENOG4112311 LUCA
GeneTreeiENSGT00390000002422
HOGENOMiHOG000293249
HOVERGENiHBG054922
InParanoidiO15318
KOiK03024
OMAiMMPRKKC
PhylomeDBiO15318
TreeFamiTF103052

Family and domain databases

InterProiView protein in InterPro
IPR024661 RNA_pol_III_Rpc31
PANTHERiPTHR15367 PTHR15367, 1 hit
PfamiView protein in Pfam
PF11705 RNA_pol_3_Rpc31, 1 hit
PIRSFiPIRSF000777 RNA_polIII_C31, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

O15318-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGNKGRGRA AYTFNIEAVG FSKGEKLPDV VLKPPPLFPD TDYKPVPLKT
60 70 80 90 100
GEGEEYMLAL KQELRETMKR MPYFIETPEE RQDIERYSKR YMKVYKEEWI
110 120 130 140 150
PDWRRLPREM MPRNKCKKAG PKPKKAKDAG KGTPLTNTED VLKKMEELEK
160 170 180 190 200
RGDGEKSDEE NEEKEGSKEK SKEGDDDDDD DAAEQEEYDE EEQEEENDYI
210 220
NSYFEDGDDF GADSDDNMDE ATY
Length:223
Mass (Da):25,914
Last modified:December 16, 2008 - v2
Checksum:i95E506D527962DD9
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6R9U7D6R9U7_HUMAN
DNA-directed RNA polymerase III sub...
POLR3G
220Annotation score:
D6R921D6R921_HUMAN
DNA-directed RNA polymerase III sub...
POLR3G
127Annotation score:
D6RJH6D6RJH6_HUMAN
DNA-directed RNA polymerase III sub...
POLR3G
100Annotation score:
D6RIT0D6RIT0_HUMAN
DNA-directed RNA polymerase III sub...
POLR3G
92Annotation score:
D6REQ0D6REQ0_HUMAN
DNA-directed RNA polymerase III sub...
POLR3G
39Annotation score:

Sequence cautioni

The sequence AAB63676 differs from that shown. Reason: Frameshift at position 214.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96K → Q in AAB63676 (PubMed:9171375).Curated1
Sequence conflicti146E → V in AAB63676 (PubMed:9171375).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93868 mRNA Translation: AAB63676.1 Frameshift.
AK295434 mRNA Translation: BAG58376.1
AC027323 Genomic DNA No translation available.
AC093510 Genomic DNA No translation available.
CH471084 Genomic DNA Translation: EAW95980.1
CCDSiCCDS43337.1
RefSeqiNP_006458.2, NM_006467.2
XP_005248461.1, XM_005248404.3
XP_011541402.1, XM_011543100.2
XP_011541403.1, XM_011543101.2
XP_011541404.1, XM_011543102.2
XP_016864446.1, XM_017008957.1
UniGeneiHs.282387

Genome annotation databases

EnsembliENST00000399107; ENSP00000382058; ENSG00000113356
ENST00000504930; ENSP00000421637; ENSG00000113356
GeneIDi10622
KEGGihsa:10622
UCSCiuc003kjq.3 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U93868 mRNA Translation: AAB63676.1 Frameshift.
AK295434 mRNA Translation: BAG58376.1
AC027323 Genomic DNA No translation available.
AC093510 Genomic DNA No translation available.
CH471084 Genomic DNA Translation: EAW95980.1
CCDSiCCDS43337.1
RefSeqiNP_006458.2, NM_006467.2
XP_005248461.1, XM_005248404.3
XP_011541402.1, XM_011543100.2
XP_011541403.1, XM_011543101.2
XP_011541404.1, XM_011543102.2
XP_016864446.1, XM_017008957.1
UniGeneiHs.282387

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115867, 15 interactors
DIPiDIP-59079N
IntActiO15318, 3 interactors
STRINGi9606.ENSP00000382058

PTM databases

iPTMnetiO15318
PhosphoSitePlusiO15318

Polymorphism and mutation databases

BioMutaiPOLR3G

Proteomic databases

EPDiO15318
MaxQBiO15318
PaxDbiO15318
PeptideAtlasiO15318
PRIDEiO15318
ProteomicsDBi48580

Protocols and materials databases

DNASUi10622
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000399107; ENSP00000382058; ENSG00000113356
ENST00000504930; ENSP00000421637; ENSG00000113356
GeneIDi10622
KEGGihsa:10622
UCSCiuc003kjq.3 human

Organism-specific databases

CTDi10622
EuPathDBiHostDB:ENSG00000113356.10
GeneCardsiPOLR3G
HGNCiHGNC:30075 POLR3G
HPAiCAB010109
HPA052658
MIMi617456 gene
neXtProtiNX_O15318
OpenTargetsiENSG00000113356
PharmGKBiPA134986024
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IKHU Eukaryota
ENOG4112311 LUCA
GeneTreeiENSGT00390000002422
HOGENOMiHOG000293249
HOVERGENiHBG054922
InParanoidiO15318
KOiK03024
OMAiMMPRKKC
PhylomeDBiO15318
TreeFamiTF103052

Enzyme and pathway databases

ReactomeiR-HSA-1834949 Cytosolic sensors of pathogen-associated DNA
R-HSA-73780 RNA Polymerase III Chain Elongation
R-HSA-73980 RNA Polymerase III Transcription Termination
R-HSA-749476 RNA Polymerase III Abortive And Retractive Initiation
R-HSA-76061 RNA Polymerase III Transcription Initiation From Type 1 Promoter
R-HSA-76066 RNA Polymerase III Transcription Initiation From Type 2 Promoter
R-HSA-76071 RNA Polymerase III Transcription Initiation From Type 3 Promoter

Miscellaneous databases

GenomeRNAii10622
PROiPR:O15318
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000113356 Expressed in 178 organ(s), highest expression level in dorsal root ganglion
CleanExiHS_POLR3G
ExpressionAtlasiO15318 baseline and differential
GenevisibleiO15318 HS

Family and domain databases

InterProiView protein in InterPro
IPR024661 RNA_pol_III_Rpc31
PANTHERiPTHR15367 PTHR15367, 1 hit
PfamiView protein in Pfam
PF11705 RNA_pol_3_Rpc31, 1 hit
PIRSFiPIRSF000777 RNA_polIII_C31, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRPC7_HUMAN
AccessioniPrimary (citable) accession number: O15318
Secondary accession number(s): A8MTH0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 16, 2008
Last modified: November 7, 2018
This is version 132 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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