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Protein

Arachidonate 15-lipoxygenase B

Gene

ALOX15B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid/(15S)-HPETE. Also acts on linoleic acid to produce 13-hydroxyoctadecadienoic acid/13-HPODE. Has no detectable 8S-lipoxygenase activity but reacts with (8S)-HPETE to produce (8S,15S)-diHPETE. May regulate progression through the cell cycle and cell proliferation. May also regulate cytokine secretion by macrophages and therefore play a role in the immune response. May also regulate macrophage differentiation into proatherogenic foam cells.7 Publications

Caution

Despite its homology with mouse Alox8 (AC O35936), it seems not to have any 8S-lipoxygenase activity on arachidonate.Curated

Catalytic activityi

Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate.
Linoleate + O2 = (9Z,11E)-13-hydroxyoctadeca-9,11-dienoate.

Cofactori

Fe cationPROSITE-ProRule annotation1 PublicationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation1 Publication

Kineticsi

  1. KM=1100 µM for arachidonate (isoform D at pH 7.4 and 20 degrees Celsius)1 Publication
  2. KM=10 µM for linoleate (isoform A at pH 7.4 and 20 degrees Celsius)1 Publication
  3. KM=25 µM for arachidonate (isoform A at pH 7.4 and 20 degrees Celsius)1 Publication
  1. Vmax=4 µmol/min/mg enzyme with arachidonate as substrate (isoform A at pH 7.4 and 20 degrees Celsius)1 Publication
  2. Vmax=2 µmol/min/mg enzyme with arachidonate as substrate (isoform D at pH 7.4 and 20 degrees Celsius)1 Publication
  3. Vmax=4 µmol/min/mg enzyme with linoleate as substrate (isoform A at pH 7.4 and 20 degrees Celsius)1 Publication

Pathwayi: hydroperoxy eicosatetraenoic acid biosynthesis

This protein is involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi15Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi17Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi39Calcium 21 Publication1
Metal bindingi40Calcium 2; via carbonyl oxygen1 Publication1
Metal bindingi42Calcium 2; via carbonyl oxygen1 Publication1
Metal bindingi44Calcium 21 Publication1
Metal bindingi85Calcium 11 Publication1
Metal bindingi86Calcium 1; via carbonyl oxygen1 Publication1
Metal bindingi373Iron; catalyticPROSITE-ProRule annotation1 Publication1
Metal bindingi378Iron; catalyticPROSITE-ProRule annotation1 Publication1
Metal bindingi553Iron; catalyticPROSITE-ProRule annotation1 Publication1
Metal bindingi676Iron; via carboxylate; catalyticPROSITE-ProRule annotation1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processLipid metabolism
LigandCalcium, Iron, Lipid-binding, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.33 2681
ReactomeiR-HSA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives
UniPathwayi
UPA00881

Chemistry databases

SwissLipidsiSLP:000000651
SLP:000001469 [O15296-1]
SLP:000001470 [O15296-4]

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 15-lipoxygenase B (EC:1.13.11.33)
Short name:
15-LOX-B
Alternative name(s):
15-lipoxygenase 2
Short name:
15-LOX-2
Arachidonate 15-lipoxygenase type II
Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-)
Gene namesi
Name:ALOX15B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000179593.15
HGNCiHGNC:434 ALOX15B
MIMi603697 gene
neXtProtiNX_O15296

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39D → A: Abolishes calcium-dependent association with membranes; when associated with A-44 and A-85. 1 Publication1
Mutagenesisi44E → A: Abolishes calcium-dependent association with membranes; when associated with A-39 and A-85. 1 Publication1
Mutagenesisi85D → A: Abolishes calcium-dependent association with membranes; when associated with A-39 and A-44. 1 Publication1
Mutagenesisi602D → Y: No effect on the stereoselectivity of the oxygenation reaction. Completely changes the stereoselectivity of the oxygenation reaction to produce (8S)-HPETE instead of (15S)-HPETE; when associated with H-603. 1 Publication1
Mutagenesisi603V → H: Changes the stereoselectivity of the oxygenation reaction. Completely changes the stereoselectivity of the oxygenation reaction to produce (8S)-HPETE instead of (15S)-HPETE; when associated with Y-602. 1 Publication1

Organism-specific databases

DisGeNETi247
OpenTargetsiENSG00000179593
PharmGKBiPA24725

Chemistry databases

ChEMBLiCHEMBL2457
GuidetoPHARMACOLOGYi1389

Polymorphism and mutation databases

BioMutaiALOX15B

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002207001 – 676Arachidonate 15-lipoxygenase BAdd BLAST676

Proteomic databases

EPDiO15296
PaxDbiO15296
PeptideAtlasiO15296
PRIDEiO15296
ProteomicsDBi48566
48567 [O15296-2]
48568 [O15296-3]
48569 [O15296-4]

PTM databases

iPTMnetiO15296
PhosphoSitePlusiO15296

Expressioni

Tissue specificityi

Expressed in hair, prostate, lung, ovary, lymph node, spinal cord and cornea.3 Publications

Inductioni

Up-regulated by UV-irradiation.1 Publication

Gene expression databases

BgeeiENSG00000179593 Expressed in 107 organ(s), highest expression level in mammalian vulva
CleanExiHS_ALOX15B
ExpressionAtlasiO15296 baseline and differential
GenevisibleiO15296 HS

Organism-specific databases

HPAiHPA010562

Interactioni

Protein-protein interaction databases

BioGridi106748, 8 interactors
IntActiO15296, 5 interactors
STRINGi9606.ENSP00000369530

Chemistry databases

BindingDBiO15296

Structurei

Secondary structure

1676
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO15296
SMRiO15296
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 124PLATPROSITE-ProRule annotationAdd BLAST123
Domaini125 – 676LipoxygenasePROSITE-ProRule annotationAdd BLAST552

Domaini

The PLAT domain can bind calcium ions; this promotes association with membranes.

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
GeneTreeiENSGT00550000074415
HOVERGENiHBG005150
InParanoidiO15296
KOiK08022
OMAiEFSIHEA
OrthoDBiEOG091G04A4
PhylomeDBiO15296
TreeFamiTF105320

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform A (identifier: O15296-1) [UniParc]FASTAAdd to basket
Also known as: 15-LOb1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEFRVRVST GEAFGAGTWD KVSVSIVGTR GESPPLPLDN LGKEFTAGAE
60 70 80 90 100
EDFQVTLPED VGRVLLLRVH KAPPVLPLLG PLAPDAWFCR WFQLTPPRGG
110 120 130 140 150
HLLFPCYQWL EGAGTLVLQE GTAKVSWADH HPVLQQQRQE ELQARQEMYQ
160 170 180 190 200
WKAYNPGWPH CLDEKTVEDL ELNIKYSTAK NANFYLQAGS AFAEMKIKGL
210 220 230 240 250
LDRKGLWRSL NEMKRIFNFR RTPAAEHAFE HWQEDAFFAS QFLNGLNPVL
260 270 280 290 300
IRRCHYLPKN FPVTDAMVAS VLGPGTSLQA ELEKGSLFLV DHGILSGIQT
310 320 330 340 350
NVINGKPQFS AAPMTLLYQS PGCGPLLPLA IQLSQTPGPN SPIFLPTDDK
360 370 380 390 400
WDWLLAKTWV RNAEFSFHEA LTHLLHSHLL PEVFTLATLR QLPHCHPLFK
410 420 430 440 450
LLIPHTRYTL HINTLARELL IVPGQVVDRS TGIGIEGFSE LIQRNMKQLN
460 470 480 490 500
YSLLCLPEDI RTRGVEDIPG YYYRDDGMQI WGAVERFVSE IIGIYYPSDE
510 520 530 540 550
SVQDDRELQA WVREIFSKGF LNQESSGIPS SLETREALVQ YVTMVIFTCS
560 570 580 590 600
AKHAAVSAGQ FDSCAWMPNL PPSMQLPPPT SKGLATCEGF IATLPPVNAT
610 620 630 640 650
CDVILALWLL SKEPGDQRPL GTYPDEHFTE EAPRRSIATF QSRLAQISRG
660 670
IQERNQGLVL PYTYLDPPLI ENSVSI
Length:676
Mass (Da):75,857
Last modified:January 11, 2011 - v3
Checksum:i4F641DF2F9D492C6
GO
Isoform B (identifier: O15296-2) [UniParc]FASTAAdd to basket
Also known as: 15-LOX2sv-b

The sequence of this isoform differs from the canonical sequence as follows:
     401-429: Missing.
     483-527: Missing.

Show »
Length:602
Mass (Da):67,345
Checksum:i8804BB44095695C2
GO
Isoform C (identifier: O15296-3) [UniParc]FASTAAdd to basket
Also known as: 15-LOX2sv-c

The sequence of this isoform differs from the canonical sequence as follows:
     561-617: FDSCAWMPNL...WLLSKEPGDQ → VRKGQRPRWQ...PKAWQHARAS
     618-676: Missing.

Show »
Length:617
Mass (Da):69,136
Checksum:i3F690488B2B766AC
GO
Isoform D (identifier: O15296-4) [UniParc]FASTAAdd to basket
Also known as: 15-LOX2sv-a, 15-LOb2

The sequence of this isoform differs from the canonical sequence as follows:
     401-429: Missing.

Show »
Length:647
Mass (Da):72,523
Checksum:iEE51EEA9221CCC4B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
I3L1D5I3L1D5_HUMAN
Arachidonate 15-lipoxygenase B
ALOX15B
664Annotation score:

Sequence cautioni

The sequence AAD37786 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271V → L in AAB61706 (PubMed:9177185).Curated1
Sequence conflicti271V → L in CAC34521 (PubMed:11350124).Curated1
Sequence conflicti338G → C in AAD37786 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061334486R → H1 PublicationCorresponds to variant dbSNP:rs9895916Ensembl.1
Natural variantiVAR_024524656Q → R4 PublicationsCorresponds to variant dbSNP:rs4792147Ensembl.1
Natural variantiVAR_024525676I → V. Corresponds to variant dbSNP:rs7225107Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003142401 – 429Missing in isoform B and isoform D. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_003143483 – 527Missing in isoform B. 1 PublicationAdd BLAST45
Alternative sequenceiVSP_003144561 – 617FDSCA…EPGDQ → VRKGQRPRWQAGGDPAPQPH SALSAFSLTPVLGCPTCHPA CSCHHPPPKAWQHARAS in isoform C. 1 PublicationAdd BLAST57
Alternative sequenceiVSP_003145618 – 676Missing in isoform C. 1 PublicationAdd BLAST59

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78294 mRNA Translation: AAB61706.1
AJ305028
, AJ305029, AJ305030, AJ305031 Genomic DNA Translation: CAC34521.1
AF468051 mRNA Translation: AAL76274.1
AF468052 mRNA Translation: AAL76275.1
AF468053 mRNA Translation: AAL76276.1
AF468054 mRNA Translation: AAL76277.1
AC129492 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90098.1
CH471108 Genomic DNA Translation: EAW90100.1
BC035217 mRNA Translation: AAH35217.1
BC063647 mRNA Translation: AAH63647.1
AF149095 Genomic DNA Translation: AAD37786.1 Sequence problems.
CCDSiCCDS11128.1 [O15296-1]
CCDS32558.1 [O15296-4]
CCDS32559.1 [O15296-2]
RefSeqiNP_001034219.1, NM_001039130.1 [O15296-4]
NP_001034220.1, NM_001039131.1 [O15296-2]
NP_001132.2, NM_001141.2 [O15296-1]
UniGeneiHs.111256

Genome annotation databases

EnsembliENST00000380173; ENSP00000369520; ENSG00000179593 [O15296-4]
ENST00000380183; ENSP00000369530; ENSG00000179593 [O15296-1]
ENST00000573359; ENSP00000460332; ENSG00000179593 [O15296-2]
GeneIDi247
KEGGihsa:247
UCSCiuc002gju.4 human [O15296-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U78294 mRNA Translation: AAB61706.1
AJ305028
, AJ305029, AJ305030, AJ305031 Genomic DNA Translation: CAC34521.1
AF468051 mRNA Translation: AAL76274.1
AF468052 mRNA Translation: AAL76275.1
AF468053 mRNA Translation: AAL76276.1
AF468054 mRNA Translation: AAL76277.1
AC129492 Genomic DNA No translation available.
CH471108 Genomic DNA Translation: EAW90098.1
CH471108 Genomic DNA Translation: EAW90100.1
BC035217 mRNA Translation: AAH35217.1
BC063647 mRNA Translation: AAH63647.1
AF149095 Genomic DNA Translation: AAD37786.1 Sequence problems.
CCDSiCCDS11128.1 [O15296-1]
CCDS32558.1 [O15296-4]
CCDS32559.1 [O15296-2]
RefSeqiNP_001034219.1, NM_001039130.1 [O15296-4]
NP_001034220.1, NM_001039131.1 [O15296-2]
NP_001132.2, NM_001141.2 [O15296-1]
UniGeneiHs.111256

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NREX-ray2.63A1-676[»]
ProteinModelPortaliO15296
SMRiO15296
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106748, 8 interactors
IntActiO15296, 5 interactors
STRINGi9606.ENSP00000369530

Chemistry databases

BindingDBiO15296
ChEMBLiCHEMBL2457
GuidetoPHARMACOLOGYi1389
SwissLipidsiSLP:000000651
SLP:000001469 [O15296-1]
SLP:000001470 [O15296-4]

PTM databases

iPTMnetiO15296
PhosphoSitePlusiO15296

Polymorphism and mutation databases

BioMutaiALOX15B

Proteomic databases

EPDiO15296
PaxDbiO15296
PeptideAtlasiO15296
PRIDEiO15296
ProteomicsDBi48566
48567 [O15296-2]
48568 [O15296-3]
48569 [O15296-4]

Protocols and materials databases

DNASUi247
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000380173; ENSP00000369520; ENSG00000179593 [O15296-4]
ENST00000380183; ENSP00000369530; ENSG00000179593 [O15296-1]
ENST00000573359; ENSP00000460332; ENSG00000179593 [O15296-2]
GeneIDi247
KEGGihsa:247
UCSCiuc002gju.4 human [O15296-1]

Organism-specific databases

CTDi247
DisGeNETi247
EuPathDBiHostDB:ENSG00000179593.15
GeneCardsiALOX15B
H-InvDBiHIX0027139
HGNCiHGNC:434 ALOX15B
HPAiHPA010562
MIMi603697 gene
neXtProtiNX_O15296
OpenTargetsiENSG00000179593
PharmGKBiPA24725
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
GeneTreeiENSGT00550000074415
HOVERGENiHBG005150
InParanoidiO15296
KOiK08022
OMAiEFSIHEA
OrthoDBiEOG091G04A4
PhylomeDBiO15296
TreeFamiTF105320

Enzyme and pathway databases

UniPathwayi
UPA00881

BRENDAi1.13.11.33 2681
ReactomeiR-HSA-2142770 Synthesis of 15-eicosatetraenoic acid derivatives

Miscellaneous databases

ChiTaRSiALOX15B human
GeneWikiiALOX15B
GenomeRNAii247
PROiPR:O15296
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000179593 Expressed in 107 organ(s), highest expression level in mammalian vulva
CleanExiHS_ALOX15B
ExpressionAtlasiO15296 baseline and differential
GenevisibleiO15296 HS

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLX15B_HUMAN
AccessioniPrimary (citable) accession number: O15296
Secondary accession number(s): D3DTR2
, Q8IYQ2, Q8TEV3, Q8TEV4, Q8TEV5, Q8TEV6, Q9UKM4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2011
Last modified: November 7, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  7. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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