UniProtKB - O15264 (MK13_HUMAN)
Mitogen-activated protein kinase 13
MAPK13
Functioni
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. MAPK13 is one of the less studied p38 MAPK isoforms. Some of the targets are downstream kinases such as MAPKAPK2, which are activated through phosphorylation and further phosphorylate additional targets. Plays a role in the regulation of protein translation by phosphorylating and inactivating EEF2K. Involved in cytoskeletal remodeling through phosphorylation of MAPT and STMN1. Mediates UV irradiation induced up-regulation of the gene expression of CXCL14. Plays an important role in the regulation of epidermal keratinocyte differentiation, apoptosis and skin tumor development. Phosphorylates the transcriptional activator MYB in response to stress which leads to rapid MYB degradation via a proteasome-dependent pathway. MAPK13 also phosphorylates and down-regulates PRKD1 during regulation of insulin secretion in pancreatic beta cells.
8 PublicationsCatalytic activityi
Cofactori
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 54 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 150 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 31 – 39 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- MAP kinase activity Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: BHF-UCL
- protein serine kinase activity Source: RHEA
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- cellular response to anisomycin Source: UniProtKB
- cellular response to hydrogen peroxide Source: UniProtKB
- cellular response to interleukin-1 Source: UniProtKB
- cellular response to sodium arsenite Source: UniProtKB
- cellular response to sorbitol Source: UniProtKB
- cellular response to UV Source: UniProtKB
- intracellular signal transduction Source: GO_Central
- peptidyl-serine phosphorylation Source: BHF-UCL
- positive regulation of inflammatory response Source: BHF-UCL
- positive regulation of interleukin-6 production Source: BHF-UCL
- response to osmotic stress Source: UniProtKB
- stress-activated MAPK cascade Source: UniProtKB
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Stress response, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 2681 |
PathwayCommonsi | O15264 |
Reactomei | R-HSA-168638, NOD1/2 Signaling Pathway R-HSA-171007, p38MAPK events R-HSA-376172, DSCAM interactions R-HSA-4420097, VEGFA-VEGFR2 Pathway |
SignaLinki | O15264 |
SIGNORi | O15264 |
Names & Taxonomyi
Protein namesi | Recommended name: Mitogen-activated protein kinase 13 (EC:2.7.11.24)Short name: MAP kinase 13 Short name: MAPK 13 Alternative name(s): Mitogen-activated protein kinase p38 delta Short name: MAP kinase p38 delta Stress-activated protein kinase 4 |
Gene namesi | Name:MAPK13 Synonyms:PRKM13, SAPK4 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6875, MAPK13 |
MIMi | 602899, gene |
neXtProti | NX_O15264 |
VEuPathDBi | HostDB:ENSG00000156711 |
Subcellular locationi
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 180 | T → A: Loss of kinase activity. 1 Publication | 1 | |
Mutagenesisi | 182 | Y → A: Loss of kinase activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5603 |
OpenTargetsi | ENSG00000156711 |
PharmGKBi | PA30620 |
Miscellaneous databases
Pharosi | O15264, Tchem |
Chemistry databases
ChEMBLi | CHEMBL2939 |
DrugBanki | DB12010, Fostamatinib DB05157, KC706 DB01017, Minocycline |
DrugCentrali | O15264 |
GuidetoPHARMACOLOGYi | 1502 |
Genetic variation databases
BioMutai | MAPK13 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186286 | 1 – 365 | Mitogen-activated protein kinase 13Add BLAST | 365 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 47 | PhosphoserineCombined sources | 1 | |
Modified residuei | 180 | Phosphothreonine; by MAP2K3, MAP2K4, MAP2K6 and MAP2K71 Publication | 1 | |
Modified residuei | 182 | Phosphotyrosine; by MAP2K3, MAP2K4, MAP2K6 and MAP2K71 Publication | 1 | |
Modified residuei | 350 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
CPTACi | CPTAC-876 CPTAC-877 |
EPDi | O15264 |
jPOSTi | O15264 |
MassIVEi | O15264 |
MaxQBi | O15264 |
PaxDbi | O15264 |
PeptideAtlasi | O15264 |
PRIDEi | O15264 |
ProteomicsDBi | 48552 [O15264-1] 65225 |
PTM databases
iPTMneti | O15264 |
PhosphoSitePlusi | O15264 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000156711, Expressed in left adrenal gland and 202 other tissues |
ExpressionAtlasi | O15264, baseline and differential |
Genevisiblei | O15264, HS |
Organism-specific databases
HPAi | ENSG00000156711, Tissue enhanced (esophagus) |
Interactioni
Subunit structurei
Interacts with MAPK8IP2.
1 PublicationBinary interactionsi
O15264
With | #Exp. | IntAct |
---|---|---|
FPR1 [P21462] | 3 | EBI-2116951,EBI-2869495 |
PRKD1 [Q15139] | 6 | EBI-2116951,EBI-1181072 |
STAT3 [P40763] | 2 | EBI-2116951,EBI-518675 |
Protein-protein interaction databases
BioGRIDi | 111589, 22 interactors |
IntActi | O15264, 105 interactors |
MINTi | O15264 |
STRINGi | 9606.ENSP00000211287 |
Chemistry databases
BindingDBi | O15264 |
Miscellaneous databases
RNActi | O15264, protein |
Structurei
Secondary structure
3D structure databases
SMRi | O15264 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O15264 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 25 – 308 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 284 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 180 – 182 | TXY | 3 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
GeneTreei | ENSGT00940000159584 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | O15264 |
OMAi | PGRDYGH |
PhylomeDBi | O15264 |
TreeFami | TF105100 |
Family and domain databases
CDDi | cd07879, STKc_p38delta, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008352, MAPK_p38-like IPR038785, p38delta IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01773, P38MAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSLIRKKGFY KQDVNKTAWE LPKTYVSPTH VGSGAYGSVC SAIDKRSGEK
60 70 80 90 100
VAIKKLSRPF QSEIFAKRAY RELLLLKHMQ HENVIGLLDV FTPASSLRNF
110 120 130 140 150
YDFYLVMPFM QTDLQKIMGM EFSEEKIQYL VYQMLKGLKY IHSAGVVHRD
160 170 180 190 200
LKPGNLAVNE DCELKILDFG LARHADAEMT GYVVTRWYRA PEVILSWMHY
210 220 230 240 250
NQTVDIWSVG CIMAEMLTGK TLFKGKDYLD QLTQILKVTG VPGTEFVQKL
260 270 280 290 300
NDKAAKSYIQ SLPQTPRKDF TQLFPRASPQ AADLLEKMLE LDVDKRLTAA
310 320 330 340 350
QALTHPFFEP FRDPEEETEA QQPFDDSLEH EKLTVDEWKQ HIYKEIVNFS
360
PIARKDSRRR SGMKL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ5R3E6 | Q5R3E6_HUMAN | Mitogen-activated protein kinase 13 | MAPK13 | 152 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 – 14 | DV → EL in AAC51374 (PubMed:9207191).Curated | 2 | |
Sequence conflicti | 39 | V → W in AAC51374 (PubMed:9207191).Curated | 1 | |
Sequence conflicti | 56 | L → P in AAC51758 (PubMed:9295308).Curated | 1 | |
Sequence conflicti | 166 | I → V in AAC51758 (PubMed:9295308).Curated | 1 | |
Sequence conflicti | 224 | K → R in AAC51758 (PubMed:9295308).Curated | 1 | |
Sequence conflicti | 224 | K → R in AAD23377 (PubMed:10066767).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_042267 | 41 | S → L1 PublicationCorresponds to variant dbSNP:rs55776345EnsemblClinVar. | 1 | |
Natural variantiVAR_042268 | 282 | A → V1 PublicationCorresponds to variant dbSNP:rs55990045Ensembl. | 1 | |
Natural variantiVAR_042269 | 300 | A → T1 PublicationCorresponds to variant dbSNP:rs41270090Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056558 | 204 – 256 | VDIWS…DKAAK → GQILHPVPATDPQEGFHSAV PTGQPPGCGPAGEDAGARRG QAPDGRAGPHPSL in isoform 2. 1 PublicationAdd BLAST | 53 | |
Alternative sequenceiVSP_056559 | 257 – 364 | Missing in isoform 2. 1 PublicationAdd BLAST | 108 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10488 mRNA Translation: CAA71512.1 U93232 mRNA Translation: AAB87639.1 AF015256 mRNA Translation: AAC51758.1 AF004709 mRNA Translation: AAC51374.1 AF092535 mRNA Translation: AAD23377.1 AF100546 mRNA Translation: AAF36772.1 BT007221 mRNA Translation: AAP35885.1 CR536490 mRNA Translation: CAG38729.1 Z95152 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03874.1 CH471081 Genomic DNA Translation: EAX03875.1 BC000433 mRNA Translation: AAH00433.1 BC001641 mRNA Translation: AAH01641.1 BC004428 mRNA Translation: AAH04428.1 BC085196 mRNA Translation: AAH85196.1 |
CCDSi | CCDS4818.1 [O15264-1] |
PIRi | JC5528 |
RefSeqi | NP_002745.1, NM_002754.4 [O15264-1] |
Genome annotation databases
Ensembli | ENST00000211287; ENSP00000211287; ENSG00000156711 ENST00000373766; ENSP00000362871; ENSG00000156711 [O15264-2] |
GeneIDi | 5603 |
KEGGi | hsa:5603 |
MANE-Selecti | ENST00000211287.9; ENSP00000211287.4; NM_002754.5; NP_002745.1 |
UCSCi | uc003ols.5, human [O15264-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Atlas of Genetics and Cytogenetics in Oncology and Haematology |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y10488 mRNA Translation: CAA71512.1 U93232 mRNA Translation: AAB87639.1 AF015256 mRNA Translation: AAC51758.1 AF004709 mRNA Translation: AAC51374.1 AF092535 mRNA Translation: AAD23377.1 AF100546 mRNA Translation: AAF36772.1 BT007221 mRNA Translation: AAP35885.1 CR536490 mRNA Translation: CAG38729.1 Z95152 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03874.1 CH471081 Genomic DNA Translation: EAX03875.1 BC000433 mRNA Translation: AAH00433.1 BC001641 mRNA Translation: AAH01641.1 BC004428 mRNA Translation: AAH04428.1 BC085196 mRNA Translation: AAH85196.1 |
CCDSi | CCDS4818.1 [O15264-1] |
PIRi | JC5528 |
RefSeqi | NP_002745.1, NM_002754.4 [O15264-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3COI | X-ray | 2.09 | A | 2-352 | [»] | |
4EYJ | X-ray | 2.10 | A | 1-352 | [»] | |
4EYM | X-ray | 2.35 | A | 1-352 | [»] | |
4MYG | X-ray | 2.59 | A/B | 1-352 | [»] | |
4YNO | X-ray | 1.70 | A | 1-352 | [»] | |
5EKN | X-ray | 2.59 | A | 1-352 | [»] | |
5EKO | X-ray | 2.00 | A | 1-352 | [»] | |
SMRi | O15264 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111589, 22 interactors |
IntActi | O15264, 105 interactors |
MINTi | O15264 |
STRINGi | 9606.ENSP00000211287 |
Chemistry databases
BindingDBi | O15264 |
ChEMBLi | CHEMBL2939 |
DrugBanki | DB12010, Fostamatinib DB05157, KC706 DB01017, Minocycline |
DrugCentrali | O15264 |
GuidetoPHARMACOLOGYi | 1502 |
PTM databases
iPTMneti | O15264 |
PhosphoSitePlusi | O15264 |
Genetic variation databases
BioMutai | MAPK13 |
Proteomic databases
CPTACi | CPTAC-876 CPTAC-877 |
EPDi | O15264 |
jPOSTi | O15264 |
MassIVEi | O15264 |
MaxQBi | O15264 |
PaxDbi | O15264 |
PeptideAtlasi | O15264 |
PRIDEi | O15264 |
ProteomicsDBi | 48552 [O15264-1] 65225 |
Protocols and materials databases
Antibodypediai | 2088, 867 antibodies from 39 providers |
DNASUi | 5603 |
Genome annotation databases
Ensembli | ENST00000211287; ENSP00000211287; ENSG00000156711 ENST00000373766; ENSP00000362871; ENSG00000156711 [O15264-2] |
GeneIDi | 5603 |
KEGGi | hsa:5603 |
MANE-Selecti | ENST00000211287.9; ENSP00000211287.4; NM_002754.5; NP_002745.1 |
UCSCi | uc003ols.5, human [O15264-1] |
Organism-specific databases
CTDi | 5603 |
DisGeNETi | 5603 |
GeneCardsi | MAPK13 |
HGNCi | HGNC:6875, MAPK13 |
HPAi | ENSG00000156711, Tissue enhanced (esophagus) |
MIMi | 602899, gene |
neXtProti | NX_O15264 |
OpenTargetsi | ENSG00000156711 |
PharmGKBi | PA30620 |
VEuPathDBi | HostDB:ENSG00000156711 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0660, Eukaryota |
GeneTreei | ENSGT00940000159584 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | O15264 |
OMAi | PGRDYGH |
PhylomeDBi | O15264 |
TreeFami | TF105100 |
Enzyme and pathway databases
BRENDAi | 2.7.11.24, 2681 |
PathwayCommonsi | O15264 |
Reactomei | R-HSA-168638, NOD1/2 Signaling Pathway R-HSA-171007, p38MAPK events R-HSA-376172, DSCAM interactions R-HSA-4420097, VEGFA-VEGFR2 Pathway |
SignaLinki | O15264 |
SIGNORi | O15264 |
Miscellaneous databases
BioGRID-ORCSi | 5603, 12 hits in 1070 CRISPR screens |
ChiTaRSi | MAPK13, human |
EvolutionaryTracei | O15264 |
GeneWikii | MAPK13 |
GenomeRNAii | 5603 |
Pharosi | O15264, Tchem |
PROi | PR:O15264 |
RNActi | O15264, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000156711, Expressed in left adrenal gland and 202 other tissues |
ExpressionAtlasi | O15264, baseline and differential |
Genevisiblei | O15264, HS |
Family and domain databases
CDDi | cd07879, STKc_p38delta, 1 hit |
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR003527, MAP_kinase_CS IPR008352, MAPK_p38-like IPR038785, p38delta IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
PRINTSi | PR01773, P38MAPKINASE |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS01351, MAPK, 1 hit PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MK13_HUMAN | |
Accessioni | O15264Primary (citable) accession number: O15264 Secondary accession number(s): O14739 Q9UNU0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | January 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 205 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families