UniProtKB - O15226 (NKRF_HUMAN)
Protein
NF-kappa-B-repressing factor
Gene
NKRF
Organism
Homo sapiens (Human)
Status
Functioni
Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.1 Publication
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 296 – 388 | Add BLAST | 93 |
GO - Molecular functioni
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: NTNU_SB
- RNA binding Source: UniProtKB
- RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
GO - Biological processi
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: NTNU_SB
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: NF-kappa-B-repressing factorShort name: NFkB-repressing factor Alternative name(s): Protein ITBA4 Transcription factor NRF |
Gene namesi | Name:NKRF Synonyms:ITBA4, NRF |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000186416.12 |
HGNCi | HGNC:19374 NKRF |
MIMi | 300440 gene |
neXtProti | NX_O15226 |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: GO_Central
- nucleus Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 55922 |
OpenTargetsi | ENSG00000186416 |
PharmGKBi | PA134990602 |
Chemistry databases
ChEMBLi | CHEMBL3163 |
Polymorphism and mutation databases
BioMutai | NKRF |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000096869 | 1 – 690 | NF-kappa-B-repressing factorAdd BLAST | 690 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 68 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 500 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 618 | PhosphoserineCombined sources | 1 | |
Cross-linki | 666 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Cross-linki | 674 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O15226 |
jPOSTi | O15226 |
MaxQBi | O15226 |
PaxDbi | O15226 |
PeptideAtlasi | O15226 |
PRIDEi | O15226 |
ProteomicsDBi | 48517 |
2D gel databases
SWISS-2DPAGEi | O15226 |
PTM databases
iPTMneti | O15226 |
PhosphoSitePlusi | O15226 |
Expressioni
Tissue specificityi
Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.
Gene expression databases
Bgeei | ENSG00000186416 Expressed in 206 organ(s), highest expression level in hypothalamus |
ExpressionAtlasi | O15226 baseline and differential |
Genevisiblei | O15226 HS |
Organism-specific databases
HPAi | HPA001476 |
Interactioni
Subunit structurei
Interacts with NF-kappa-B. Interacts with XRN2 (PubMed:24462208).1 Publication
Binary interactionsi
Protein-protein interaction databases
BioGridi | 121002, 61 interactors |
DIPi | DIP-34570N |
IntActi | O15226, 44 interactors |
MINTi | O15226 |
STRINGi | 9606.ENSP00000442308 |
Structurei
3D structure databases
ProteinModelPortali | O15226 |
SMRi | O15226 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 551 – 596 | G-patchPROSITE-ProRule annotationAdd BLAST | 46 | |
Domaini | 600 – 664 | R3HPROSITE-ProRule annotationAdd BLAST | 65 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 296 | Active repression domainAdd BLAST | 296 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 25 – 45 | Nuclear localization signalAdd BLAST | 21 |
Phylogenomic databases
eggNOGi | KOG1721 Eukaryota COG5048 LUCA |
GeneTreei | ENSGT00940000157256 |
HOGENOMi | HOG000230930 |
HOVERGENi | HBG052630 |
InParanoidi | O15226 |
OMAi | VCQIGMP |
OrthoDBi | 1179335at2759 |
PhylomeDBi | O15226 |
TreeFami | TF326321 |
Family and domain databases
CDDi | cd02640 R3H_NRF, 1 hit |
Gene3Di | 3.30.1370.50, 1 hit |
InterProi | View protein in InterPro IPR014720 dsRBD_dom IPR000467 G_patch_dom IPR001374 R3H_dom IPR036867 R3H_dom_sf IPR034071 R3H_NRF |
Pfami | View protein in Pfam PF01585 G-patch, 1 hit PF01424 R3H, 1 hit |
SMARTi | View protein in SMART SM00358 DSRM, 2 hits SM00443 G_patch, 1 hit SM00393 R3H, 1 hit |
SUPFAMi | SSF82708 SSF82708, 1 hit |
PROSITEi | View protein in PROSITE PS50174 G_PATCH, 1 hit PS51061 R3H, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: O15226-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK
60 70 80 90 100
FHARPRFEPV HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY
110 120 130 140 150
TQDSFSIQDG NSQYCDSSGF ILTKDQPVTA NMYFDSGNPA PSTTSQQANS
160 170 180 190 200
QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT
210 220 230 240 250
YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY
260 270 280 290 300
LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI
310 320 330 340 350
GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI
360 370 380 390 400
GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA
410 420 430 440 450
AADEALKILQ KTQPTYPSVK SSQCHTGSSP RGSGKKKDIK DLVVYENSSN
460 470 480 490 500
PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKTVK
510 520 530 540 550
YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS AEEAYKQQIK
560 570 580 590 600
EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
610 620 630 640 650
IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH
660 670 680 690
GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN
Sequence cautioni
The sequence CAB56459 differs from that shown. Reason: Frameshift at positions 378, 405, 410, 441, 502, 516, 588, 599, 601 and 681.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 71 | R → G in CAB56459 (PubMed:10562553).Curated | 1 | |
Sequence conflicti | 453 | C → G in CAA68976 (PubMed:9224902).Curated | 1 | |
Sequence conflicti | 614 – 615 | AR → ES in CAB56459 (PubMed:10562553).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_047377 | 1 | M → MGFMLPLIFRYSPRLM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011812 mRNA Translation: CAB56459.1 Frameshift. AY208891 mRNA Translation: AAP43025.1 AC004913 Genomic DNA No translation available. CH471161 Genomic DNA Translation: EAW89859.1 BC040379 mRNA Translation: AAH40379.1 BC047878 mRNA Translation: AAH47878.1 AL539002 mRNA No translation available. Y07707 mRNA Translation: CAA68976.1 |
CCDSi | CCDS35375.1 [O15226-1] CCDS55486.1 [O15226-2] |
RefSeqi | NP_001166958.1, NM_001173487.1 [O15226-2] NP_001166959.1, NM_001173488.1 [O15226-1] NP_060014.2, NM_017544.3 [O15226-1] XP_011529667.1, XM_011531365.2 [O15226-1] |
UniGenei | Hs.437084 |
Genome annotation databases
Ensembli | ENST00000304449; ENSP00000304803; ENSG00000186416 [O15226-1] ENST00000371527; ENSP00000360582; ENSG00000186416 [O15226-1] ENST00000542113; ENSP00000442308; ENSG00000186416 [O15226-2] |
GeneIDi | 55922 |
KEGGi | hsa:55922 |
UCSCi | uc004erq.3 human [O15226-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ011812 mRNA Translation: CAB56459.1 Frameshift. AY208891 mRNA Translation: AAP43025.1 AC004913 Genomic DNA No translation available. CH471161 Genomic DNA Translation: EAW89859.1 BC040379 mRNA Translation: AAH40379.1 BC047878 mRNA Translation: AAH47878.1 AL539002 mRNA No translation available. Y07707 mRNA Translation: CAA68976.1 |
CCDSi | CCDS35375.1 [O15226-1] CCDS55486.1 [O15226-2] |
RefSeqi | NP_001166958.1, NM_001173487.1 [O15226-2] NP_001166959.1, NM_001173488.1 [O15226-1] NP_060014.2, NM_017544.3 [O15226-1] XP_011529667.1, XM_011531365.2 [O15226-1] |
UniGenei | Hs.437084 |
3D structure databases
ProteinModelPortali | O15226 |
SMRi | O15226 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 121002, 61 interactors |
DIPi | DIP-34570N |
IntActi | O15226, 44 interactors |
MINTi | O15226 |
STRINGi | 9606.ENSP00000442308 |
Chemistry databases
ChEMBLi | CHEMBL3163 |
PTM databases
iPTMneti | O15226 |
PhosphoSitePlusi | O15226 |
Polymorphism and mutation databases
BioMutai | NKRF |
2D gel databases
SWISS-2DPAGEi | O15226 |
Proteomic databases
EPDi | O15226 |
jPOSTi | O15226 |
MaxQBi | O15226 |
PaxDbi | O15226 |
PeptideAtlasi | O15226 |
PRIDEi | O15226 |
ProteomicsDBi | 48517 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENST00000304449; ENSP00000304803; ENSG00000186416 [O15226-1] ENST00000371527; ENSP00000360582; ENSG00000186416 [O15226-1] ENST00000542113; ENSP00000442308; ENSG00000186416 [O15226-2] |
GeneIDi | 55922 |
KEGGi | hsa:55922 |
UCSCi | uc004erq.3 human [O15226-1] |
Organism-specific databases
CTDi | 55922 |
DisGeNETi | 55922 |
EuPathDBi | HostDB:ENSG00000186416.12 |
GeneCardsi | NKRF |
HGNCi | HGNC:19374 NKRF |
HPAi | HPA001476 |
MIMi | 300440 gene |
neXtProti | NX_O15226 |
OpenTargetsi | ENSG00000186416 |
PharmGKBi | PA134990602 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721 Eukaryota COG5048 LUCA |
GeneTreei | ENSGT00940000157256 |
HOGENOMi | HOG000230930 |
HOVERGENi | HBG052630 |
InParanoidi | O15226 |
OMAi | VCQIGMP |
OrthoDBi | 1179335at2759 |
PhylomeDBi | O15226 |
TreeFami | TF326321 |
Miscellaneous databases
GeneWikii | NKRF_(gene) |
GenomeRNAii | 55922 |
PROi | PR:O15226 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000186416 Expressed in 206 organ(s), highest expression level in hypothalamus |
ExpressionAtlasi | O15226 baseline and differential |
Genevisiblei | O15226 HS |
Family and domain databases
CDDi | cd02640 R3H_NRF, 1 hit |
Gene3Di | 3.30.1370.50, 1 hit |
InterProi | View protein in InterPro IPR014720 dsRBD_dom IPR000467 G_patch_dom IPR001374 R3H_dom IPR036867 R3H_dom_sf IPR034071 R3H_NRF |
Pfami | View protein in Pfam PF01585 G-patch, 1 hit PF01424 R3H, 1 hit |
SMARTi | View protein in SMART SM00358 DSRM, 2 hits SM00443 G_patch, 1 hit SM00393 R3H, 1 hit |
SUPFAMi | SSF82708 SSF82708, 1 hit |
PROSITEi | View protein in PROSITE PS50174 G_PATCH, 1 hit PS51061 R3H, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | NKRF_HUMAN | |
Accessioni | O15226Primary (citable) accession number: O15226 Secondary accession number(s): G3V1N1, Q4VC41, Q9UJ91 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | August 15, 2003 | |
Last modified: | February 13, 2019 | |
This is version 187 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- Human chromosome X
Human chromosome X: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot