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Protein

NF-kappa-B-repressing factor

Gene

NKRF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Interacts with a specific negative regulatory element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression of certain NK-kappa-B responsive genes. Involved in the constitutive silencing of the interferon beta promoter, independently of the virus-induced signals, and in the inhibition of the basal and cytokine-induced iNOS promoter activity. Also involved in the regulation of IL-8 transcription.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi296 – 388Add BLAST93

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B-repressing factor
Short name:
NFkB-repressing factor
Alternative name(s):
Protein ITBA4
Transcription factor NRF
Gene namesi
Name:NKRF
Synonyms:ITBA4, NRF
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

EuPathDBiHostDB:ENSG00000186416.12
HGNCiHGNC:19374 NKRF
MIMi300440 gene
neXtProtiNX_O15226

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi55922
OpenTargetsiENSG00000186416
PharmGKBiPA134990602

Chemistry databases

ChEMBLiCHEMBL3163

Polymorphism and mutation databases

BioMutaiNKRF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968691 – 690NF-kappa-B-repressing factorAdd BLAST690

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki68Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei618PhosphoserineCombined sources1
Cross-linki666Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki674Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO15226
MaxQBiO15226
PaxDbiO15226
PeptideAtlasiO15226
PRIDEiO15226
ProteomicsDBi48517

2D gel databases

SWISS-2DPAGEiO15226

PTM databases

iPTMnetiO15226
PhosphoSitePlusiO15226

Expressioni

Tissue specificityi

Widely and constitutively expressed. Expressed at lower level in colon, peripheral blood lymphocytes, lung and kidney.

Gene expression databases

BgeeiENSG00000186416 Expressed in 206 organ(s), highest expression level in hypothalamus
CleanExiHS_NKRF
ExpressionAtlasiO15226 baseline and differential
GenevisibleiO15226 HS

Organism-specific databases

HPAiHPA001476

Interactioni

Subunit structurei

Interacts with NF-kappa-B. Interacts with XRN2 (PubMed:24462208).1 Publication

Binary interactionsi

Protein-protein interaction databases

BioGridi121002, 53 interactors
DIPiDIP-34570N
IntActiO15226, 42 interactors
MINTiO15226
STRINGi9606.ENSP00000442308

Structurei

3D structure databases

ProteinModelPortaliO15226
SMRiO15226
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 596G-patchPROSITE-ProRule annotationAdd BLAST46
Domaini600 – 664R3HPROSITE-ProRule annotationAdd BLAST65

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 296Active repression domainAdd BLAST296

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi25 – 45Nuclear localization signalAdd BLAST21

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00530000063234
HOGENOMiHOG000230930
HOVERGENiHBG052630
InParanoidiO15226
OMAiVCQIGMP
OrthoDBiEOG091G0HNZ
PhylomeDBiO15226
TreeFamiTF326321

Family and domain databases

CDDicd02640 R3H_NRF, 1 hit
Gene3Di3.30.1370.50, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR000467 G_patch_dom
IPR001374 R3H_dom
IPR036867 R3H_dom_sf
IPR034071 R3H_NRF
PfamiView protein in Pfam
PF01585 G-patch, 1 hit
PF01424 R3H, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 2 hits
SM00443 G_patch, 1 hit
SM00393 R3H, 1 hit
SUPFAMiSSF82708 SSF82708, 1 hit
PROSITEiView protein in PROSITE
PS50174 G_PATCH, 1 hit
PS51061 R3H, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O15226-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEKILQMAEG IDIGEMPSYD LVLSKPSKGQ KRHLSTCDGQ NPPKKQAGSK
60 70 80 90 100
FHARPRFEPV HFVASSSKDE RQEDPYGPQT KEVNEQTHFA SMPRDIYQDY
110 120 130 140 150
TQDSFSIQDG NSQYCDSSGF ILTKDQPVTA NMYFDSGNPA PSTTSQQANS
160 170 180 190 200
QSTPEPSPSQ TFPESVVAEK QYFIEKLTAT IWKNLSNPEM TSGSDKINYT
210 220 230 240 250
YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY ACEVRCQNIY
260 270 280 290 300
LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHT FGEDLVVCQI
310 320 330 340 350
GMSSYEFPPA LKPPEDLVVL GKDASGQPIF NASAKHWTNF VITENANDAI
360 370 380 390 400
GILNNSASFN KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA
410 420 430 440 450
AADEALKILQ KTQPTYPSVK SSQCHTGSSP RGSGKKKDIK DLVVYENSSN
460 470 480 490 500
PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC KVILESEVIA EAVGVKKTVK
510 520 530 540 550
YEAAGEAVKT LKKTQPTVIN NLKKGAVEDV ISRNEIQGRS AEEAYKQQIK
560 570 580 590 600
EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
610 620 630 640 650
IAKRDIEQII RNYARSESHT DLTFSRELTN DERKQIHQIA QKYGLKSKSH
660 670 680 690
GVGHDRYLVV GRKRRKEDLL DQLKQEGQVG HYELVMPQAN
Length:690
Mass (Da):77,673
Last modified:August 15, 2003 - v2
Checksum:i3067A52671A7AE8F
GO
Isoform 2 (identifier: O15226-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MGFMLPLIFRYSPRLM

Note: No experimental confirmation available.
Show »
Length:705
Mass (Da):79,496
Checksum:i48B245E4929BD14B
GO

Sequence cautioni

The sequence AL539002 differs from that shown. Probable cloning artifact.Curated
The sequence CAB56459 differs from that shown. Reason: Frameshift at positions 378, 405, 410, 441, 502, 516, 588, 599, 601 and 681.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti71R → G in CAB56459 (PubMed:10562553).Curated1
Sequence conflicti453C → G in CAA68976 (PubMed:9224902).Curated1
Sequence conflicti614 – 615AR → ES in CAB56459 (PubMed:10562553).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0473771M → MGFMLPLIFRYSPRLM in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011812 mRNA Translation: CAB56459.1 Frameshift.
AY208891 mRNA Translation: AAP43025.1
AC004913 Genomic DNA No translation available.
CH471161 Genomic DNA Translation: EAW89859.1
BC040379 mRNA Translation: AAH40379.1
BC047878 mRNA Translation: AAH47878.1
AL539002 mRNA No translation available.
Y07707 mRNA Translation: CAA68976.1
CCDSiCCDS35375.1 [O15226-1]
CCDS55486.1 [O15226-2]
RefSeqiNP_001166958.1, NM_001173487.1 [O15226-2]
NP_001166959.1, NM_001173488.1 [O15226-1]
NP_060014.2, NM_017544.3 [O15226-1]
XP_011529667.1, XM_011531365.2 [O15226-1]
UniGeneiHs.437084

Genome annotation databases

EnsembliENST00000304449; ENSP00000304803; ENSG00000186416 [O15226-1]
ENST00000371527; ENSP00000360582; ENSG00000186416 [O15226-1]
ENST00000542113; ENSP00000442308; ENSG00000186416 [O15226-2]
GeneIDi55922
KEGGihsa:55922
UCSCiuc004erq.3 human [O15226-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011812 mRNA Translation: CAB56459.1 Frameshift.
AY208891 mRNA Translation: AAP43025.1
AC004913 Genomic DNA No translation available.
CH471161 Genomic DNA Translation: EAW89859.1
BC040379 mRNA Translation: AAH40379.1
BC047878 mRNA Translation: AAH47878.1
AL539002 mRNA No translation available.
Y07707 mRNA Translation: CAA68976.1
CCDSiCCDS35375.1 [O15226-1]
CCDS55486.1 [O15226-2]
RefSeqiNP_001166958.1, NM_001173487.1 [O15226-2]
NP_001166959.1, NM_001173488.1 [O15226-1]
NP_060014.2, NM_017544.3 [O15226-1]
XP_011529667.1, XM_011531365.2 [O15226-1]
UniGeneiHs.437084

3D structure databases

ProteinModelPortaliO15226
SMRiO15226
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121002, 53 interactors
DIPiDIP-34570N
IntActiO15226, 42 interactors
MINTiO15226
STRINGi9606.ENSP00000442308

Chemistry databases

ChEMBLiCHEMBL3163

PTM databases

iPTMnetiO15226
PhosphoSitePlusiO15226

Polymorphism and mutation databases

BioMutaiNKRF

2D gel databases

SWISS-2DPAGEiO15226

Proteomic databases

EPDiO15226
MaxQBiO15226
PaxDbiO15226
PeptideAtlasiO15226
PRIDEiO15226
ProteomicsDBi48517

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304449; ENSP00000304803; ENSG00000186416 [O15226-1]
ENST00000371527; ENSP00000360582; ENSG00000186416 [O15226-1]
ENST00000542113; ENSP00000442308; ENSG00000186416 [O15226-2]
GeneIDi55922
KEGGihsa:55922
UCSCiuc004erq.3 human [O15226-1]

Organism-specific databases

CTDi55922
DisGeNETi55922
EuPathDBiHostDB:ENSG00000186416.12
GeneCardsiNKRF
HGNCiHGNC:19374 NKRF
HPAiHPA001476
MIMi300440 gene
neXtProtiNX_O15226
OpenTargetsiENSG00000186416
PharmGKBiPA134990602
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00530000063234
HOGENOMiHOG000230930
HOVERGENiHBG052630
InParanoidiO15226
OMAiVCQIGMP
OrthoDBiEOG091G0HNZ
PhylomeDBiO15226
TreeFamiTF326321

Miscellaneous databases

GeneWikiiNKRF_(gene)
GenomeRNAii55922
PROiPR:O15226
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186416 Expressed in 206 organ(s), highest expression level in hypothalamus
CleanExiHS_NKRF
ExpressionAtlasiO15226 baseline and differential
GenevisibleiO15226 HS

Family and domain databases

CDDicd02640 R3H_NRF, 1 hit
Gene3Di3.30.1370.50, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR000467 G_patch_dom
IPR001374 R3H_dom
IPR036867 R3H_dom_sf
IPR034071 R3H_NRF
PfamiView protein in Pfam
PF01585 G-patch, 1 hit
PF01424 R3H, 1 hit
SMARTiView protein in SMART
SM00358 DSRM, 2 hits
SM00443 G_patch, 1 hit
SM00393 R3H, 1 hit
SUPFAMiSSF82708 SSF82708, 1 hit
PROSITEiView protein in PROSITE
PS50174 G_PATCH, 1 hit
PS51061 R3H, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNKRF_HUMAN
AccessioniPrimary (citable) accession number: O15226
Secondary accession number(s): G3V1N1, Q4VC41, Q9UJ91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 15, 2003
Last modified: November 7, 2018
This is version 184 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
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