Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutathione S-transferase A4

Gene

GSTA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).2 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei9GlutathioneBy similarity1
Binding sitei212SubstrateCurated1

GO - Molecular functioni

  • glutathione transferase activity Source: UniProtKB
  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase A4 (EC:2.5.1.18)
Alternative name(s):
GST class-alpha member 4
Glutathione S-transferase A4-4
Gene namesi
Name:GSTA4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000170899.10
HGNCiHGNC:4629 GSTA4
MIMi605450 gene
neXtProtiNX_O15217

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi9Y → F: Reduces catalytic activity 70-fold. 1 Publication1
Mutagenesisi212Y → A, F or S: Strongly reduced activity towards 4-hydroxynon-2-enal and 1-chloro-2,4-dinitrobenzene. 1 Publication1

Organism-specific databases

DisGeNETi2941
OpenTargetsiENSG00000170899
PharmGKBiPA29019

Chemistry databases

ChEMBLiCHEMBL4933
DrugBankiDB00143 Glutathione

Polymorphism and mutation databases

BioMutaiGSTA4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001857861 – 222Glutathione S-transferase A4Add BLAST222

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO15217
MaxQBiO15217
PaxDbiO15217
PeptideAtlasiO15217
PRIDEiO15217
ProteomicsDBi48514

PTM databases

iPTMnetiO15217
PhosphoSitePlusiO15217

Expressioni

Tissue specificityi

Expressed at a high level in brain, placenta, and skeletal muscle and much lower in lung and liver.

Gene expression databases

BgeeiENSG00000170899 Expressed in 236 organ(s), highest expression level in metanephros
CleanExiHS_GSTA4
ExpressionAtlasiO15217 baseline and differential
GenevisibleiO15217 HS

Organism-specific databases

HPAiHPA048934

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109196, 10 interactors
IntActiO15217, 8 interactors
MINTiO15217
STRINGi9606.ENSP00000359998

Structurei

Secondary structure

1222
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO15217
SMRiO15217
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15217

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 83GST N-terminalAdd BLAST81
Domaini85 – 208GST C-terminalAdd BLAST124

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni54 – 55Glutathione bindingBy similarity2
Regioni67 – 68Glutathione bindingBy similarity2

Sequence similaritiesi

Belongs to the GST superfamily. Alpha family.Curated

Phylogenomic databases

eggNOGiKOG1695 Eukaryota
ENOG4111VAU LUCA
GeneTreeiENSGT00670000097856
HOGENOMiHOG000115734
HOVERGENiHBG053749
InParanoidiO15217
KOiK00799
OMAiNEELPKW
OrthoDBiEOG091G0O3D
PhylomeDBiO15217
TreeFamiTF105321

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR003080 GST_alpha
IPR004046 GST_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01266 GSTRNSFRASEA
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O15217-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAARPKLHYP NGRGRMESVR WVLAAAGVEF DEEFLETKEQ LYKLQDGNHL
60 70 80 90 100
LFQQVPMVEI DGMKLVQTRS ILHYIADKHN LFGKNLKERT LIDMYVEGTL
110 120 130 140 150
DLLELLIMHP FLKPDDQQKE VVNMAQKAII RYFPVFEKIL RGHGQSFLVG
160 170 180 190 200
NQLSLADVIL LQTILALEEK IPNILSAFPF LQEYTVKLSN IPTIKRFLEP
210 220
GSKKKPPPDE IYVRTVYNIF RP
Length:222
Mass (Da):25,704
Last modified:January 1, 1998 - v1
Checksum:i1BD98B41E413BB4E
GO
Isoform 2 (identifier: O15217-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-93: Missing.

Show »
Length:129
Mass (Da):14,884
Checksum:i746CA2AA5C2BCA24
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5JW88Q5JW88_HUMAN
Glutathione S-transferase A4
GSTA4
89Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_022210100L → P1 PublicationCorresponds to variant dbSNP:rs45551133Ensembl.1
Natural variantiVAR_022211163T → A1 PublicationCorresponds to variant dbSNP:rs4147617Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0564731 – 93Missing in isoform 2. 2 PublicationsAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13047 mRNA Translation: CAA73482.1
AF020918 mRNA Translation: AAD04711.1
AF025887 mRNA Translation: AAC39695.1
AF125271 mRNA Translation: AAD27704.1
AF125272 mRNA Translation: AAD27705.1
AF125273 mRNA Translation: AAD27706.1
AF050059
, AF050054, AF050055, AF050056, AF050057, AF050058 Genomic DNA Translation: AAC72706.1
AY878121 Genomic DNA Translation: AAW56075.1
AK315369 mRNA Translation: BAG37762.1
CR749474 mRNA Translation: CAH18304.1
AL121969 Genomic DNA No translation available.
AL162581 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04393.1
CH471081 Genomic DNA Translation: EAX04394.1
CH471081 Genomic DNA Translation: EAX04395.1
CH471081 Genomic DNA Translation: EAX04396.1
CH471081 Genomic DNA Translation: EAX04397.1
BC015523 mRNA Translation: AAH15523.1
BC063439 mRNA Translation: AAH63439.1
CCDSiCCDS4948.1 [O15217-1]
RefSeqiNP_001503.1, NM_001512.3 [O15217-1]
XP_005249092.1, XM_005249035.3 [O15217-1]
UniGeneiHs.485557

Genome annotation databases

EnsembliENST00000370959; ENSP00000359998; ENSG00000170899 [O15217-1]
ENST00000370960; ENSP00000359999; ENSG00000170899 [O15217-2]
ENST00000370963; ENSP00000360002; ENSG00000170899 [O15217-1]
GeneIDi2941
KEGGihsa:2941
UCSCiuc003pbf.4 human [O15217-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y13047 mRNA Translation: CAA73482.1
AF020918 mRNA Translation: AAD04711.1
AF025887 mRNA Translation: AAC39695.1
AF125271 mRNA Translation: AAD27704.1
AF125272 mRNA Translation: AAD27705.1
AF125273 mRNA Translation: AAD27706.1
AF050059
, AF050054, AF050055, AF050056, AF050057, AF050058 Genomic DNA Translation: AAC72706.1
AY878121 Genomic DNA Translation: AAW56075.1
AK315369 mRNA Translation: BAG37762.1
CR749474 mRNA Translation: CAH18304.1
AL121969 Genomic DNA No translation available.
AL162581 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX04393.1
CH471081 Genomic DNA Translation: EAX04394.1
CH471081 Genomic DNA Translation: EAX04395.1
CH471081 Genomic DNA Translation: EAX04396.1
CH471081 Genomic DNA Translation: EAX04397.1
BC015523 mRNA Translation: AAH15523.1
BC063439 mRNA Translation: AAH63439.1
CCDSiCCDS4948.1 [O15217-1]
RefSeqiNP_001503.1, NM_001512.3 [O15217-1]
XP_005249092.1, XM_005249035.3 [O15217-1]
UniGeneiHs.485557

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1GULX-ray2.70A/B/C/D/E/F/G/H1-222[»]
1GUMX-ray3.00A/B/C/D/E/F/G/H1-222[»]
3IK7X-ray1.97A/B/C/D1-222[»]
ProteinModelPortaliO15217
SMRiO15217
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109196, 10 interactors
IntActiO15217, 8 interactors
MINTiO15217
STRINGi9606.ENSP00000359998

Chemistry databases

ChEMBLiCHEMBL4933
DrugBankiDB00143 Glutathione

PTM databases

iPTMnetiO15217
PhosphoSitePlusiO15217

Polymorphism and mutation databases

BioMutaiGSTA4

Proteomic databases

EPDiO15217
MaxQBiO15217
PaxDbiO15217
PeptideAtlasiO15217
PRIDEiO15217
ProteomicsDBi48514

Protocols and materials databases

DNASUi2941
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370959; ENSP00000359998; ENSG00000170899 [O15217-1]
ENST00000370960; ENSP00000359999; ENSG00000170899 [O15217-2]
ENST00000370963; ENSP00000360002; ENSG00000170899 [O15217-1]
GeneIDi2941
KEGGihsa:2941
UCSCiuc003pbf.4 human [O15217-1]

Organism-specific databases

CTDi2941
DisGeNETi2941
EuPathDBiHostDB:ENSG00000170899.10
GeneCardsiGSTA4
HGNCiHGNC:4629 GSTA4
HPAiHPA048934
MIMi605450 gene
neXtProtiNX_O15217
OpenTargetsiENSG00000170899
PharmGKBiPA29019
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1695 Eukaryota
ENOG4111VAU LUCA
GeneTreeiENSGT00670000097856
HOGENOMiHOG000115734
HOVERGENiHBG053749
InParanoidiO15217
KOiK00799
OMAiNEELPKW
OrthoDBiEOG091G0O3D
PhylomeDBiO15217
TreeFamiTF105321

Enzyme and pathway databases

BRENDAi2.5.1.18 2681
ReactomeiR-HSA-156590 Glutathione conjugation

Miscellaneous databases

ChiTaRSiGSTA4 human
EvolutionaryTraceiO15217
GeneWikiiGSTA4
GenomeRNAii2941
PROiPR:O15217
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000170899 Expressed in 236 organ(s), highest expression level in metanephros
CleanExiHS_GSTA4
ExpressionAtlasiO15217 baseline and differential
GenevisibleiO15217 HS

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR003080 GST_alpha
IPR004046 GST_C
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF02798 GST_N, 1 hit
PRINTSiPR01266 GSTRNSFRASEA
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSTA4_HUMAN
AccessioniPrimary (citable) accession number: O15217
Secondary accession number(s): B2RD15
, Q5T7Q8, Q6P4G1, Q9BX18, Q9H414
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again