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Protein

Phospholipid scramblase 1

Gene

PLSCR1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system.
May play a role in the antiviral response of interferon (IFN) by amplifying and enhancing the IFN response through increased expression of select subset of potent antiviral genes. May contribute to cytokine-regulated cell proliferation and differentiation.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processAntiviral defense
LigandCalcium

Enzyme and pathway databases

BRENDAi3.6.99.B1 2681
SignaLinkiO15162
SIGNORiO15162

Protein family/group databases

TCDBi9.A.36.1.1 the ca(2+)-dependent phospholipid scramblase (scramblase) family

Chemistry databases

SwissLipidsiSLP:000000332

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipid scramblase 1
Short name:
PL scramblase 1
Alternative name(s):
Ca(2+)-dependent phospholipid scramblase 1
Erythrocyte phospholipid scramblase
MmTRA1b
Gene namesi
Name:PLSCR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000188313.12
HGNCiHGNC:9092 PLSCR1
MIMi604170 gene
neXtProtiNX_O15162

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 288CytoplasmicAdd BLAST288
Transmembranei289 – 305HelicalSequence analysisAdd BLAST17
Topological domaini306 – 318ExtracellularAdd BLAST13

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69Y → F: Decrease in phosphorylation. 1
Mutagenesisi74Y → F: Decrease in phosphorylation. 1
Mutagenesisi161T → A: No induction by PKC. 1
Mutagenesisi184 – 189CCCPCC → AAAPAA: No palmitoylation; constitutively localizes in the nucleus. 2 Publications6
Mutagenesisi273D → A: Reduces the Ca(2+)-dependent phospholipid scrambling. 1
Mutagenesisi275D → A: Complete inactivation of the Ca(2+)-dependent phospholipid scrambling. 1
Mutagenesisi277F → A: Reduces the Ca(2+)-dependent phospholipid scrambling. 1
Mutagenesisi279I → A: Reduces the Ca(2+)-dependent phospholipid scrambling. 1
Mutagenesisi281F → A: Complete inactivation of the Ca(2+)-dependent phospholipid scrambling. 1
Mutagenesisi284D → A: Reduces the Ca(2+)-dependent phospholipid scrambling. 1

Organism-specific databases

DisGeNETi5359
OpenTargetsiENSG00000188313
PharmGKBiPA33419

Polymorphism and mutation databases

BioMutaiPLSCR1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007841 – 318Phospholipid scramblase 1Add BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69Phosphotyrosine; by ABL1 Publication1
Modified residuei74Phosphotyrosine; by ABL1 Publication1
Modified residuei161Phosphothreonine; by PKC1 Publication1
Lipidationi184S-palmitoyl cysteine1 Publication1
Lipidationi185S-palmitoyl cysteine1 Publication1
Lipidationi186S-palmitoyl cysteine1 Publication1
Lipidationi188S-palmitoyl cysteine1 Publication1
Lipidationi189S-palmitoyl cysteine1 Publication1

Post-translational modificationi

Phosphorylated by OXSR1 in the presence of RELT.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiO15162
MaxQBiO15162
PaxDbiO15162
PeptideAtlasiO15162
PRIDEiO15162
ProteomicsDBi48483

PTM databases

iPTMnetiO15162
PhosphoSitePlusiO15162
SwissPalmiO15162

Expressioni

Tissue specificityi

Expressed in platelets, erythrocyte membranes, lymphocytes, spleen, thymus, prostate, testis, uterus, intestine, colon, heart, placenta, lung, liver, kidney and pancreas. Not detected in brain and skeletal muscle.

Inductioni

By phosphorylation by PKC. Induced by IFNB1/IFN-beta in response to a viral infection.1 Publication

Gene expression databases

BgeeiENSG00000188313 Expressed in 225 organ(s), highest expression level in muscle layer of sigmoid colon
CleanExiHS_PLSCR1
ExpressionAtlasiO15162 baseline and differential
GenevisibleiO15162 HS

Organism-specific databases

HPAiCAB070421
HPA068923

Interactioni

Subunit structurei

Interacts with ABL (PubMed:11390389). Interacts with RELT, RELL1 and RELL2 (PubMed:22052202). Interacts with OXSR1 in the presence of RELT (PubMed:22052202).2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111373, 140 interactors
IntActiO15162, 138 interactors
MINTiO15162
STRINGi9606.ENSP00000345494

Structurei

3D structure databases

ProteinModelPortaliO15162
SMRiO15162
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO15162

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 84Proline-rich domain (PRD)Add BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi18 – 26SH3-binding 1Sequence analysis9
Motifi22 – 25PPXY motif 1Sequence analysis4
Motifi33 – 36PPXY motif 2Sequence analysis4
Motifi42 – 50SH3-binding 2Sequence analysis9
Motifi84 – 92SH3-binding 3Sequence analysis9
Motifi257 – 266Nuclear localization signal1 Publication10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi181 – 189Cys-rich9

Domaini

The N-terminal proline-rich domain (PRD) is required for phospholipid scramblase activity.

Sequence similaritiesi

Belongs to the phospholipid scramblase family.Curated

Keywords - Domaini

Repeat, SH3-binding, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0621 Eukaryota
ENOG410XSYS LUCA
GeneTreeiENSGT00390000002884
HOGENOMiHOG000237356
HOVERGENiHBG019157
InParanoidiO15162
OMAiIPWMPAP
OrthoDBiEOG091G0CUL
PhylomeDBiO15162
TreeFamiTF314939

Family and domain databases

InterProiView protein in InterPro
IPR005552 Scramblase
PANTHERiPTHR23248 PTHR23248, 1 hit
PfamiView protein in Pfam
PF03803 Scramblase, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O15162-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDKQNSQMNA SHPETNLPVG YPPQYPPTAF QGPPGYSGYP GPQVSYPPPP
60 70 80 90 100
AGHSGPGPAG FPVPNQPVYN QPVYNQPVGA AGVPWMPAPQ PPLNCPPGLE
110 120 130 140 150
YLSQIDQILI HQQIELLEVL TGFETNNKYE IKNSFGQRVY FAAEDTDCCT
160 170 180 190 200
RNCCGPSRPF TLRIIDNMGQ EVITLERPLR CSSCCCPCCL QEIEIQAPPG
210 220 230 240 250
VPIGYVIQTW HPCLPKFTIQ NEKREDVLKI SGPCVVCSCC GDVDFEIKSL
260 270 280 290 300
DEQCVVGKIS KHWTGILREA FTDADNFGIQ FPLDLDVKMK AVMIGACFLI
310
DFMFFESTGS QEQKSGVW
Length:318
Mass (Da):35,049
Last modified:January 1, 1998 - v1
Checksum:i9860744DEC40616E
GO
Isoform 2 (identifier: O15162-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MDKQNSQMNASHPETNLPVGYPP → MLLTRKQTCQLGILLSIHRQHSK
     24-104: Missing.

Note: No experimental confirmation available.
Show »
Length:237
Mass (Da):26,825
Checksum:i61B33BDD238A5519
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JE06C9JE06_HUMAN
Phospholipid scramblase
PLSCR1
249Annotation score:
C9J9P4C9J9P4_HUMAN
Phospholipid scramblase
PLSCR1
248Annotation score:
C9J7K9C9J7K9_HUMAN
Phospholipid scramblase
PLSCR1 hCG_17108
311Annotation score:
C9J0H3C9J0H3_HUMAN
Phospholipid scramblase
PLSCR1
171Annotation score:
H7C5I5H7C5I5_HUMAN
Phospholipid scramblase
PLSCR1
171Annotation score:
F2Z3F2F2Z3F2_HUMAN
Phospholipid scramblase 1
PLSCR1
41Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034388262H → Y. Corresponds to variant dbSNP:rs343320Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0552371 – 23MDKQN…VGYPP → MLLTRKQTCQLGILLSIHRQ HSK in isoform 2. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_05523824 – 104Missing in isoform 2. 1 PublicationAdd BLAST81

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098642 mRNA Translation: AAC99413.1
AB006746 mRNA Translation: BAA32568.1
AF224492 Genomic DNA Translation: AAF80593.1
AK300181 mRNA Translation: BAG61960.1
AC069528 Genomic DNA No translation available.
AC116544 Genomic DNA No translation available.
AK313377 mRNA Translation: BAG36175.1
CH471052 Genomic DNA Translation: EAW78926.1
BC021100 mRNA Translation: AAH21100.1
BC032718 mRNA Translation: AAH32718.1
CCDSiCCDS3135.1 [O15162-1]
CCDS87152.1 [O15162-2]
PIRiJE0284
RefSeqiNP_066928.1, NM_021105.2 [O15162-1]
XP_005247595.1, XM_005247538.3 [O15162-2]
XP_011511206.1, XM_011512904.1 [O15162-1]
XP_011511208.1, XM_011512906.2 [O15162-2]
XP_011511209.1, XM_011512907.2 [O15162-2]
XP_016862118.1, XM_017006629.1 [O15162-1]
UniGeneiHs.130759

Genome annotation databases

EnsembliENST00000342435; ENSP00000345494; ENSG00000188313 [O15162-1]
ENST00000448787; ENSP00000411675; ENSG00000188313 [O15162-2]
GeneIDi5359
KEGGihsa:5359
UCSCiuc003evx.5 human [O15162-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

Scramblase entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF098642 mRNA Translation: AAC99413.1
AB006746 mRNA Translation: BAA32568.1
AF224492 Genomic DNA Translation: AAF80593.1
AK300181 mRNA Translation: BAG61960.1
AC069528 Genomic DNA No translation available.
AC116544 Genomic DNA No translation available.
AK313377 mRNA Translation: BAG36175.1
CH471052 Genomic DNA Translation: EAW78926.1
BC021100 mRNA Translation: AAH21100.1
BC032718 mRNA Translation: AAH32718.1
CCDSiCCDS3135.1 [O15162-1]
CCDS87152.1 [O15162-2]
PIRiJE0284
RefSeqiNP_066928.1, NM_021105.2 [O15162-1]
XP_005247595.1, XM_005247538.3 [O15162-2]
XP_011511206.1, XM_011512904.1 [O15162-1]
XP_011511208.1, XM_011512906.2 [O15162-2]
XP_011511209.1, XM_011512907.2 [O15162-2]
XP_016862118.1, XM_017006629.1 [O15162-1]
UniGeneiHs.130759

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y2AX-ray2.20P257-266[»]
ProteinModelPortaliO15162
SMRiO15162
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111373, 140 interactors
IntActiO15162, 138 interactors
MINTiO15162
STRINGi9606.ENSP00000345494

Chemistry databases

SwissLipidsiSLP:000000332

Protein family/group databases

TCDBi9.A.36.1.1 the ca(2+)-dependent phospholipid scramblase (scramblase) family

PTM databases

iPTMnetiO15162
PhosphoSitePlusiO15162
SwissPalmiO15162

Polymorphism and mutation databases

BioMutaiPLSCR1

Proteomic databases

EPDiO15162
MaxQBiO15162
PaxDbiO15162
PeptideAtlasiO15162
PRIDEiO15162
ProteomicsDBi48483

Protocols and materials databases

DNASUi5359
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342435; ENSP00000345494; ENSG00000188313 [O15162-1]
ENST00000448787; ENSP00000411675; ENSG00000188313 [O15162-2]
GeneIDi5359
KEGGihsa:5359
UCSCiuc003evx.5 human [O15162-1]

Organism-specific databases

CTDi5359
DisGeNETi5359
EuPathDBiHostDB:ENSG00000188313.12
GeneCardsiPLSCR1
HGNCiHGNC:9092 PLSCR1
HPAiCAB070421
HPA068923
MIMi604170 gene
neXtProtiNX_O15162
OpenTargetsiENSG00000188313
PharmGKBiPA33419
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0621 Eukaryota
ENOG410XSYS LUCA
GeneTreeiENSGT00390000002884
HOGENOMiHOG000237356
HOVERGENiHBG019157
InParanoidiO15162
OMAiIPWMPAP
OrthoDBiEOG091G0CUL
PhylomeDBiO15162
TreeFamiTF314939

Enzyme and pathway databases

BRENDAi3.6.99.B1 2681
SignaLinkiO15162
SIGNORiO15162

Miscellaneous databases

ChiTaRSiPLSCR1 human
EvolutionaryTraceiO15162
GeneWikiiPLSCR1
GenomeRNAii5359
PROiPR:O15162
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000188313 Expressed in 225 organ(s), highest expression level in muscle layer of sigmoid colon
CleanExiHS_PLSCR1
ExpressionAtlasiO15162 baseline and differential
GenevisibleiO15162 HS

Family and domain databases

InterProiView protein in InterPro
IPR005552 Scramblase
PANTHERiPTHR23248 PTHR23248, 1 hit
PfamiView protein in Pfam
PF03803 Scramblase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLS1_HUMAN
AccessioniPrimary (citable) accession number: O15162
Secondary accession number(s): B2R8H8, B4DTE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: January 1, 1998
Last modified: October 10, 2018
This is version 183 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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