UniProtKB - O15156 (ZBT7B_HUMAN)
Zinc finger and BTB domain-containing protein 7B
ZBTB7B
Functioni
Transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. Exerts distinct biological functions in the mammary epithelial cells and T cells in a tissue-specific manner. Necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. Cross-antagonism between ZBTB7B and CBF complexes are determinative to CD4 versus CD8 cell fate decision. Suppresses RUNX3 expression and imposes CD4+ lineage fate by inducing the SOCS suppressors of cytokine signaling. induces, as a transcriptional activator, SOCS genes expression which represses RUNX3 expression and promotes the CD4+ lineage fate. During CD4 lineage commitment, associates with multiple sites at the CD8 locus, acting as a negative regulator of the CD8 promoter and enhancers by epigenetic silencing through the recruitment of class II histone deacetylases, such as HDAC4 and HDAC5, to these loci. Regulates the development of IL17-producing CD1d-restricted naural killer (NK) T cells. Also functions as an important metabolic regulator in the lactating mammary glands. Critical feed-forward regulator of insulin signaling in mammary gland lactation, directly regulates expression of insulin receptor substrate-1 (IRS-1) and insulin-induced Akt-mTOR-SREBP signaling (By similarity).
Transcriptional repressor of the collagen COL1A1 and COL1A2 genes. May also function as a repressor of fibronectin and possibly other extracellular matrix genes (PubMed:9370309).
Potent driver of brown fat development, thermogenesis and cold-induced beige fat formation. Recruits the brown fat lncRNA 1 (Blnc1):HNRNPU ribonucleoprotein complex to activate thermogenic gene expression in brown and beige adipocytes (By similarity).
By similarity1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 346 – 368 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 374 – 396 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 402 – 424 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 430 – 454 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 25 |
GO - Molecular functioni
- cis-regulatory region sequence-specific DNA binding Source: UniProtKB
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: Ensembl
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: GO_Central
- histone deacetylase binding Source: Ensembl
- metal ion binding Source: UniProtKB-KW
- protein homodimerization activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: ARUK-UCL
GO - Biological processi
- adaptive thermogenesis Source: UniProtKB
- ectoderm development Source: ProtInc
- lactation Source: UniProtKB
- negative regulation of CD8-positive, alpha-beta T cell differentiation Source: UniProtKB
- negative regulation of gene expression Source: Ensembl
- negative regulation of NK T cell proliferation Source: UniProtKB
- negative regulation of T-helper 17 cell differentiation Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- NK T cell differentiation Source: UniProtKB
- positive regulation of brown fat cell differentiation Source: UniProtKB
- positive regulation of CD4-positive, alpha-beta T cell differentiation Source: UniProtKB
- positive regulation of cold-induced thermogenesis Source: YuBioLab
- positive regulation of gene expression Source: UniProtKB
- positive regulation of histone deacetylation Source: UniProtKB
- positive regulation of insulin receptor signaling pathway Source: UniProtKB
- positive regulation of interleukin-17 production Source: UniProtKB
- positive regulation of SREBP signaling pathway Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: GO_Central
- response to insulin Source: UniProtKB
- transcription by RNA polymerase II Source: ProtInc
Keywordsi
Molecular function | Developmental protein, DNA-binding, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | O15156 |
SignaLinki | O15156 |
Names & Taxonomyi
Protein namesi | Recommended name: Zinc finger and BTB domain-containing protein 7BCuratedAlternative name(s): Krueppel-related zinc finger protein cKrox Short name: hcKrox T-helper-inducing POZ/Krueppel-like factor Zinc finger and BTB domain-containing protein 15 Zinc finger protein 67 homolog Short name: Zfp-67 Zinc finger protein 857B Zinc finger protein Th-POK |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:18668, ZBTB7B |
MIMi | 607646, gene |
neXtProti | NX_O15156 |
VEuPathDBi | HostDB:ENSG00000160685 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Nucleus
- nucleoplasm Source: HPA
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 51043 |
OpenTargetsi | ENSG00000160685 |
PharmGKBi | PA38630 |
Miscellaneous databases
Pharosi | O15156, Tbio |
Genetic variation databases
BioMutai | ZBTB7B |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000047719 | 1 – 539 | Zinc finger and BTB domain-containing protein 7BAdd BLAST | 539 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 150 | PhosphoserineCombined sources | 1 | |
Modified residuei | 206 | N6-acetyllysine; by EP300; alternateBy similarity | 1 | |
Cross-linki | 206 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 212 | N6-acetyllysine; by EP300; alternateBy similarity | 1 | |
Cross-linki | 212 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 335 | N6-acetyllysine; by EP300; alternateBy similarity | 1 | |
Cross-linki | 335 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 369 | PhosphothreonineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O15156 |
jPOSTi | O15156 |
MassIVEi | O15156 |
MaxQBi | O15156 |
PaxDbi | O15156 |
PeptideAtlasi | O15156 |
PRIDEi | O15156 |
ProteomicsDBi | 48480 [O15156-1] |
PTM databases
iPTMneti | O15156 |
PhosphoSitePlusi | O15156 |
Expressioni
Gene expression databases
Bgeei | ENSG00000160685, Expressed in esophagus mucosa and 202 other tissues |
Genevisiblei | O15156, HS |
Organism-specific databases
HPAi | ENSG00000160685, Low tissue specificity |
Interactioni
Subunit structurei
Homodimerizes.
Interacts with NCL, NEDD4 and YBX1.
Interacts with HNRNPU (via RNA-binding RGG-box region); the interaction facilitates the recruitment of long non-coding RNA Blnc1 by ZBTB7B.
Interacts with HDAC4 and HDAC5; the interaction allows the recruitment of HDAC4 and HDAC5 on CD8 loci for deacetylation and possible inhibition of CD8 genes expression.
By similarityBinary interactionsi
O15156
Isoform 2 [O15156-2]
GO - Molecular functioni
- histone deacetylase binding Source: Ensembl
- protein homodimerization activity Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 119242, 62 interactors |
IntActi | O15156, 28 interactors |
MINTi | O15156 |
STRINGi | 9606.ENSP00000406286 |
Miscellaneous databases
RNActi | O15156, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 34 – 115 | BTBPROSITE-ProRule annotationAdd BLAST | 82 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 171 – 308 | DisorderedSequence analysisAdd BLAST | 138 | |
Regioni | 344 – 400 | Required for interaction with and acetylation by EP300By similarityAdd BLAST | 57 | |
Regioni | 458 – 486 | DisorderedSequence analysisAdd BLAST | 29 | |
Regioni | 501 – 539 | DisorderedSequence analysisAdd BLAST | 39 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 180 – 197 | Pro residuesSequence analysisAdd BLAST | 18 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 346 – 368 | C2H2-type 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 374 – 396 | C2H2-type 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 402 – 424 | C2H2-type 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Zinc fingeri | 430 – 454 | C2H2-type 4; atypicalPROSITE-ProRule annotationAdd BLAST | 25 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000160219 |
HOGENOMi | CLU_025627_0_0_1 |
InParanoidi | O15156 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | O15156 |
TreeFami | TF331824 |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGSPEDDLIG IPFPDHSSEL LSCLNEQRQL GHLCDLTIRT QGLEYRTHRA
60 70 80 90 100
VLAACSHYFK KLFTEGGGGA VMGAGGSGTA TGGAGAGVCE LDFVGPEALG
110 120 130 140 150
ALLEFAYTAT LTTSSANMPA VLQAARLLEI PCVIAACMEI LQGSGLEAPS
160 170 180 190 200
PDEDDCERAR QYLEAFATAT ASGVPNGEDS PPQVPLPPPP PPPPRPVARR
210 220 230 240 250
SRKPRKAFLQ TKGARANHLV PEVPTVPAHP LTYEEEEVAG RVGSSGGSGP
260 270 280 290 300
GDSYSPPTGT ASPPEGPQSY EPYEGEEEEE ELVYPPAYGL AQGGGPPLSP
310 320 330 340 350
EELGSDEDAI DPDLMAYLSS LHQDNLAPGL DSQDKLVRKR RSQMPQECPV
360 370 380 390 400
CHKIIHGAGK LPRHMRTHTG EKPFACEVCG VRFTRNDKLK IHMRKHTGER
410 420 430 440 450
PYSCPHCPAR FLHSYDLKNH MHLHTGDRPY ECHLCHKAFA KEDHLQRHLK
460 470 480 490 500
GQNCLEVRTR RRRKDDAPPH YPPPSTAAAS PAGLDLSNGH LDTFRLSLAR
510 520 530
FWEQSAPTGP PVSTPGPPDD DEEEGAPTTP QAEGAMESS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 90 | E → K in BAG65517 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 235 | E → G in BAG65517 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 437 | K → R in AAC51847 (PubMed:9370309).Curated | 1 | |
Sequence conflicti | 480 | S → F in AAC51847 (PubMed:9370309).Curated | 1 | |
Sequence conflicti | 509 – 510 | GP → WA in AAC51847 (PubMed:9370309).Curated | 2 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_044721 | 1 | M → MLQPGPHPPSPQAAAPGEAW PGPSQAPWQSLEEKM in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF007833 mRNA Translation: AAC51847.1 AK304762 mRNA Translation: BAG65517.1 CR749303 mRNA Translation: CAH18158.1 AL451085 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53149.1 CH471121 Genomic DNA Translation: EAW53150.1 CH471121 Genomic DNA Translation: EAW53151.1 BC012070 mRNA Translation: AAH12070.1 |
CCDSi | CCDS1081.1 [O15156-1] CCDS58030.1 [O15156-2] |
RefSeqi | NP_001239335.1, NM_001252406.2 [O15156-2] NP_001243384.1, NM_001256455.1 [O15156-1] XP_006711412.2, XM_006711349.2 XP_006711416.1, XM_006711353.1 XP_006711417.1, XM_006711354.1 [O15156-1] XP_006711419.1, XM_006711356.3 [O15156-1] XP_006711420.1, XM_006711357.1 [O15156-1] XP_006711421.1, XM_006711358.1 [O15156-1] XP_006711422.1, XM_006711359.2 [O15156-1] XP_011507900.1, XM_011509598.2 [O15156-2] XP_011507901.1, XM_011509599.2 [O15156-1] |
Genome annotation databases
Ensembli | ENST00000292176; ENSP00000292176; ENSG00000160685 ENST00000368426; ENSP00000357411; ENSG00000160685 ENST00000417934; ENSP00000406286; ENSG00000160685 [O15156-2] ENST00000535420; ENSP00000438647; ENSG00000160685 |
GeneIDi | 51043 |
KEGGi | hsa:51043 |
MANE-Selecti | ENST00000535420.6; ENSP00000438647.1; NM_001256455.2; NP_001243384.1 |
UCSCi | uc001fgk.5, human [O15156-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF007833 mRNA Translation: AAC51847.1 AK304762 mRNA Translation: BAG65517.1 CR749303 mRNA Translation: CAH18158.1 AL451085 Genomic DNA No translation available. CH471121 Genomic DNA Translation: EAW53149.1 CH471121 Genomic DNA Translation: EAW53150.1 CH471121 Genomic DNA Translation: EAW53151.1 BC012070 mRNA Translation: AAH12070.1 |
CCDSi | CCDS1081.1 [O15156-1] CCDS58030.1 [O15156-2] |
RefSeqi | NP_001239335.1, NM_001252406.2 [O15156-2] NP_001243384.1, NM_001256455.1 [O15156-1] XP_006711412.2, XM_006711349.2 XP_006711416.1, XM_006711353.1 XP_006711417.1, XM_006711354.1 [O15156-1] XP_006711419.1, XM_006711356.3 [O15156-1] XP_006711420.1, XM_006711357.1 [O15156-1] XP_006711421.1, XM_006711358.1 [O15156-1] XP_006711422.1, XM_006711359.2 [O15156-1] XP_011507900.1, XM_011509598.2 [O15156-2] XP_011507901.1, XM_011509599.2 [O15156-1] |
3D structure databases
SMRi | O15156 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 119242, 62 interactors |
IntActi | O15156, 28 interactors |
MINTi | O15156 |
STRINGi | 9606.ENSP00000406286 |
PTM databases
iPTMneti | O15156 |
PhosphoSitePlusi | O15156 |
Genetic variation databases
BioMutai | ZBTB7B |
Proteomic databases
EPDi | O15156 |
jPOSTi | O15156 |
MassIVEi | O15156 |
MaxQBi | O15156 |
PaxDbi | O15156 |
PeptideAtlasi | O15156 |
PRIDEi | O15156 |
ProteomicsDBi | 48480 [O15156-1] |
Protocols and materials databases
ABCDi | O15156, 4 sequenced antibodies |
Antibodypediai | 20407, 284 antibodies from 38 providers |
DNASUi | 51043 |
Genome annotation databases
Ensembli | ENST00000292176; ENSP00000292176; ENSG00000160685 ENST00000368426; ENSP00000357411; ENSG00000160685 ENST00000417934; ENSP00000406286; ENSG00000160685 [O15156-2] ENST00000535420; ENSP00000438647; ENSG00000160685 |
GeneIDi | 51043 |
KEGGi | hsa:51043 |
MANE-Selecti | ENST00000535420.6; ENSP00000438647.1; NM_001256455.2; NP_001243384.1 |
UCSCi | uc001fgk.5, human [O15156-1] |
Organism-specific databases
CTDi | 51043 |
DisGeNETi | 51043 |
GeneCardsi | ZBTB7B |
HGNCi | HGNC:18668, ZBTB7B |
HPAi | ENSG00000160685, Low tissue specificity |
MIMi | 607646, gene |
neXtProti | NX_O15156 |
OpenTargetsi | ENSG00000160685 |
PharmGKBi | PA38630 |
VEuPathDBi | HostDB:ENSG00000160685 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1721, Eukaryota |
GeneTreei | ENSGT00940000160219 |
HOGENOMi | CLU_025627_0_0_1 |
InParanoidi | O15156 |
OrthoDBi | 1318335at2759 |
PhylomeDBi | O15156 |
TreeFami | TF331824 |
Enzyme and pathway databases
PathwayCommonsi | O15156 |
SignaLinki | O15156 |
Miscellaneous databases
BioGRID-ORCSi | 51043, 41 hits in 1112 CRISPR screens |
ChiTaRSi | ZBTB7B, human |
GeneWikii | ZBTB7B |
GenomeRNAii | 51043 |
Pharosi | O15156, Tbio |
PROi | PR:O15156 |
RNActi | O15156, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000160685, Expressed in esophagus mucosa and 202 other tissues |
Genevisiblei | O15156, HS |
Family and domain databases
Gene3Di | 3.30.710.10, 1 hit |
InterProi | View protein in InterPro IPR000210, BTB/POZ_dom IPR011333, SKP1/BTB/POZ_sf IPR036236, Znf_C2H2_sf IPR013087, Znf_C2H2_type |
Pfami | View protein in Pfam PF00651, BTB, 1 hit PF00096, zf-C2H2, 2 hits |
SMARTi | View protein in SMART SM00225, BTB, 1 hit SM00355, ZnF_C2H2, 4 hits |
SUPFAMi | SSF54695, SSF54695, 1 hit SSF57667, SSF57667, 2 hits |
PROSITEi | View protein in PROSITE PS50097, BTB, 1 hit PS00028, ZINC_FINGER_C2H2_1, 3 hits PS50157, ZINC_FINGER_C2H2_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ZBT7B_HUMAN | |
Accessioni | O15156Primary (citable) accession number: O15156 Secondary accession number(s): B4E3K5 Q96EP2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | August 16, 2004 | |
Last modified: | February 23, 2022 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot