UniProtKB - O15145 (ARPC3_HUMAN)
Protein
Actin-related protein 2/3 complex subunit 3
Gene
ARPC3
Organism
Homo sapiens (Human)
Status
Functioni
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).2 Publications
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
- structural constituent of cytoskeleton Source: FlyBase
GO - Biological processi
- actin polymerization-dependent cell motility Source: UniProtKB
- Arp2/3 complex-mediated actin nucleation Source: FlyBase
- cellular response to nerve growth factor stimulus Source: Ensembl
- ephrin receptor signaling pathway Source: Reactome
- Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
- membrane organization Source: Reactome
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
PathwayCommonsi | O15145 |
Reactomei | R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-3928662, EPHB-mediated forward signaling R-HSA-5663213, RHO GTPases Activate WASPs and WAVEs R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-9664422, FCGR3A-mediated phagocytosis |
SIGNORi | O15145 |
Names & Taxonomyi
Protein namesi | Recommended name: Actin-related protein 2/3 complex subunit 3Alternative name(s): Arp2/3 complex 21 kDa subunit Short name: p21-ARC |
Gene namesi | Name:ARPC3 Synonyms:ARC21 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:706, ARPC3 |
MIMi | 604225, gene |
neXtProti | NX_O15145 |
VEuPathDBi | HostDB:ENSG00000111229.15 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 2 Publications
Nucleus
- Nucleus 1 Publication
Other locations
- Cell projection 2 Publications
Cytoskeleton
- actin cytoskeleton Source: ProtInc
- Arp2/3 protein complex Source: FlyBase
- filamentous actin Source: Ensembl
Cytosol
- cytosol Source: Reactome
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Other locations
- focal adhesion Source: UniProtKB
- growth cone leading edge Source: Ensembl
- lamellipodium Source: UniProtKB
- membrane Source: UniProtKB
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 10094 |
OpenTargetsi | ENSG00000111229 |
PharmGKBi | PA25000 |
Miscellaneous databases
Pharosi | O15145, Tbio |
Chemistry databases
DrugBanki | DB08236, (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one DB08235, N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide |
Genetic variation databases
BioMutai | ARPC3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000124042 | 2 – 178 | Actin-related protein 2/3 complex subunit 3Add BLAST | 177 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 14 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | ||
Modified residuei | 47 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 56 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 61 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O15145 |
jPOSTi | O15145 |
MassIVEi | O15145 |
MaxQBi | O15145 |
PaxDbi | O15145 |
PeptideAtlasi | O15145 |
PRIDEi | O15145 |
ProteomicsDBi | 48470 |
TopDownProteomicsi | O15145 |
2D gel databases
OGPi | O15145 |
PTM databases
iPTMneti | O15145 |
PhosphoSitePlusi | O15145 |
SwissPalmi | O15145 |
Expressioni
Gene expression databases
Bgeei | ENSG00000111229, Expressed in monocyte and 146 other tissues |
ExpressionAtlasi | O15145, baseline and differential |
Genevisiblei | O15145, HS |
Organism-specific databases
HPAi | ENSG00000111229, Low tissue specificity |
Interactioni
Subunit structurei
Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.
2 PublicationsBinary interactionsi
Hide detailsO15145
GO - Molecular functioni
- actin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 115401, 122 interactors |
CORUMi | O15145 |
DIPi | DIP-33187N |
IntActi | O15145, 85 interactors |
MINTi | O15145 |
STRINGi | 9606.ENSP00000228825 |
Miscellaneous databases
RNActi | O15145, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ARPC3 family.Curated
Phylogenomic databases
eggNOGi | KOG3155, Eukaryota |
GeneTreei | ENSGT00390000018018 |
HOGENOMi | CLU_094365_1_0_1 |
InParanoidi | O15145 |
OMAi | RPSKWWL |
OrthoDBi | 1451115at2759 |
PhylomeDBi | O15145 |
TreeFami | TF314598 |
Family and domain databases
Gene3Di | 1.10.1760.10, 1 hit |
InterProi | View protein in InterPro IPR007204, ARPC3 IPR036753, ARPC3_sf |
PANTHERi | PTHR12391, PTHR12391, 1 hit |
Pfami | View protein in Pfam PF04062, P21-Arc, 1 hit |
PIRSFi | PIRSF016315, ARP2/3_P21-Arc, 1 hit |
SUPFAMi | SSF69060, SSF69060, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
O15145-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPAYHSSLMD PDTKLIGNMA LLPIRSQFKG PAPRETKDTD IVDEAIYYFK
60 70 80 90 100
ANVFFKNYEI KNEADRTLIY ITLYISECLK KLQKCNSKSQ GEKEMYTLGI
110 120 130 140 150
TNFPIPGEPG FPLNAIYAKP ANKQEDEVMR AYLQQLRQET GLRLCEKVFD
160 170
PQNDKPSKWW TCFVKRQFMN KSLSGPGQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF8VR50 | F8VR50_HUMAN | Actin-related protein 2/3 complex s... | ARPC3 | 84 | Annotation score: | ||
C9JZD1 | C9JZD1_HUMAN | Actin-related protein 2/3 complex s... | ARPC3 | 99 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 134 | Q → P in AAB61466 (PubMed:9359840).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004561 mRNA Translation: AAB61466.1 AF006086 mRNA Translation: AAB64191.1 CR407667 mRNA Translation: CAG28595.1 BC067747 mRNA Translation: AAH67747.1 BC078162 mRNA Translation: AAH78162.1 |
CCDSi | CCDS9146.1 |
RefSeqi | NP_001265485.1, NM_001278556.1 NP_001274151.1, NM_001287222.1 |
Genome annotation databases
Ensembli | ENST00000228825; ENSP00000228825; ENSG00000111229 |
GeneIDi | 10094 |
KEGGi | hsa:10094 |
UCSCi | uc001tqq.5, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF004561 mRNA Translation: AAB61466.1 AF006086 mRNA Translation: AAB64191.1 CR407667 mRNA Translation: CAG28595.1 BC067747 mRNA Translation: AAH67747.1 BC078162 mRNA Translation: AAH78162.1 |
CCDSi | CCDS9146.1 |
RefSeqi | NP_001265485.1, NM_001278556.1 NP_001274151.1, NM_001287222.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6UHC | electron microscopy | 3.90 | E | 1-178 | [»] | |
6YW6 | electron microscopy | 4.20 | E | 1-178 | [»] | |
6YW7 | electron microscopy | 4.50 | E | 1-178 | [»] | |
SMRi | O15145 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115401, 122 interactors |
CORUMi | O15145 |
DIPi | DIP-33187N |
IntActi | O15145, 85 interactors |
MINTi | O15145 |
STRINGi | 9606.ENSP00000228825 |
Chemistry databases
DrugBanki | DB08236, (2S)-2-(3-bromophenyl)-3-(5-chloro-2-hydroxyphenyl)-1,3-thiazolidin-4-one DB08235, N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide |
PTM databases
iPTMneti | O15145 |
PhosphoSitePlusi | O15145 |
SwissPalmi | O15145 |
Genetic variation databases
BioMutai | ARPC3 |
2D gel databases
OGPi | O15145 |
Proteomic databases
EPDi | O15145 |
jPOSTi | O15145 |
MassIVEi | O15145 |
MaxQBi | O15145 |
PaxDbi | O15145 |
PeptideAtlasi | O15145 |
PRIDEi | O15145 |
ProteomicsDBi | 48470 |
TopDownProteomicsi | O15145 |
Protocols and materials databases
Antibodypediai | 1504, 239 antibodies |
DNASUi | 10094 |
Genome annotation databases
Ensembli | ENST00000228825; ENSP00000228825; ENSG00000111229 |
GeneIDi | 10094 |
KEGGi | hsa:10094 |
UCSCi | uc001tqq.5, human |
Organism-specific databases
CTDi | 10094 |
DisGeNETi | 10094 |
GeneCardsi | ARPC3 |
HGNCi | HGNC:706, ARPC3 |
HPAi | ENSG00000111229, Low tissue specificity |
MIMi | 604225, gene |
neXtProti | NX_O15145 |
OpenTargetsi | ENSG00000111229 |
PharmGKBi | PA25000 |
VEuPathDBi | HostDB:ENSG00000111229.15 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3155, Eukaryota |
GeneTreei | ENSGT00390000018018 |
HOGENOMi | CLU_094365_1_0_1 |
InParanoidi | O15145 |
OMAi | RPSKWWL |
OrthoDBi | 1451115at2759 |
PhylomeDBi | O15145 |
TreeFami | TF314598 |
Enzyme and pathway databases
PathwayCommonsi | O15145 |
Reactomei | R-HSA-2029482, Regulation of actin dynamics for phagocytic cup formation R-HSA-3928662, EPHB-mediated forward signaling R-HSA-5663213, RHO GTPases Activate WASPs and WAVEs R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-9664422, FCGR3A-mediated phagocytosis |
SIGNORi | O15145 |
Miscellaneous databases
BioGRID-ORCSi | 10094, 459 hits in 970 CRISPR screens |
ChiTaRSi | ARPC3, human |
GeneWikii | ARPC3 |
GenomeRNAii | 10094 |
Pharosi | O15145, Tbio |
PROi | PR:O15145 |
RNActi | O15145, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000111229, Expressed in monocyte and 146 other tissues |
ExpressionAtlasi | O15145, baseline and differential |
Genevisiblei | O15145, HS |
Family and domain databases
Gene3Di | 1.10.1760.10, 1 hit |
InterProi | View protein in InterPro IPR007204, ARPC3 IPR036753, ARPC3_sf |
PANTHERi | PTHR12391, PTHR12391, 1 hit |
Pfami | View protein in Pfam PF04062, P21-Arc, 1 hit |
PIRSFi | PIRSF016315, ARP2/3_P21-Arc, 1 hit |
SUPFAMi | SSF69060, SSF69060, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARPC3_HUMAN | |
Accessioni | O15145Primary (citable) accession number: O15145 Secondary accession number(s): O00554 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 178 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families