ARPC3

Functionⁱ

Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF) (PubMed:9230079). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility (PubMed:9230079). In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA (PubMed:29925947). The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs) (PubMed:29925947).2 Publications

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW
structural constituent of cytoskeleton
Source: FlyBase
Inferred from direct assayⁱ
- 11741539

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin polymerization-dependent cell motility
Source: UniProtKB
Traceable author statementⁱ
- 9230079
Arp2/3 complex-mediated actin nucleation
Source: FlyBase
Inferred from direct assayⁱ
- 11741539
cellular response to nerve growth factor stimulus Source: Ensembl
ephrin receptor signaling pathway Source: Reactome
Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
membrane organization Source: Reactome

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Actin-binding

Molecular function

Actin-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation R-HSA-3928662 EPHB-mediated forward signaling R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs R-HSA-8856828 Clathrin-mediated endocytosis
SIGNORⁱ	O15145

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Actin-related protein 2/3 complex subunit 3 Alternative name(s): Arp2/3 complex 21 kDa subunit Short name: p21-ARC
Gene namesⁱ	Name:ARPC3 Synonyms:ARC21
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 12

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000111229.15
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:706 ARPC3
MIMⁱ	604225 gene
neXtProtⁱ	NX_O15145

Keywords - Cellular componentⁱ

Cell projection, Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10094
Open Targets More...OpenTargetsⁱ	ENSG00000111229
PharmGKBⁱ	PA25000

Chemistry databases

Drug and drug target database More...DrugBankⁱ	DB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

DrugBankⁱ

DB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		Removed1 Publication
ChainⁱPRO_0000124042	2 – 178	Actin-related protein 2/3 complex subunit 3Add BLAST		177

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Cross-linkⁱ	14	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residueⁱ	47	PhosphotyrosineCombined sources	1
Modified residueⁱ	56	N6-acetyllysineCombined sources	1
Modified residueⁱ	61	N6-acetyllysineCombined sources	1

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	O15145
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O15145
PaxDbⁱ	O15145
PeptideAtlas More...PeptideAtlasⁱ	O15145
PRIDEⁱ	O15145
ProteomicsDBⁱ	48470
TopDownProteomicsⁱ	O15145

2D gel databases

USC-OGP 2-DE database More...OGPⁱ	O15145

OGPⁱ

O15145

PTM databases

iPTMnetⁱ	O15145
PhosphoSitePlusⁱ	O15145
SwissPalmⁱ	O15145

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000111229 Expressed in 125 organ(s), highest expression level in leukocyte
ExpressionAtlasⁱ	O15145 baseline and differential
Genevisibleⁱ	O15145 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA006550

HPAⁱ

HPA006550

Interactionⁱ

Subunit structureⁱ

Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.2 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
ARPC1B	O15143	11	EBI-351829,EBI-1044647
ARPC4	P59998	4	EBI-351829,EBI-351872
GOLGA2	Q08379	5	EBI-351829,EBI-618309
HOXA3	O43365	5	EBI-351829,EBI-8643838
NRIP1	P48552	3	EBI-351829,EBI-746484
WASL	O00401	5	EBI-351829,EBI-957615

GO - Molecular functionⁱ

actin binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	115401, 89 interactors
CORUMⁱ	O15145
Database of interacting proteins More...DIPⁱ	DIP-33187N
IntActⁱ	O15145, 71 interactors
Molecular INTeraction database More...MINTⁱ	O15145
STRINGⁱ	9606.ENSP00000228825

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O15145
SMRⁱ	O15145
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ARPC3 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3155 Eukaryota ENOG4111FTG LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000018018
HOGENOMⁱ	HOG000161465
HOVERGENⁱ	HBG050581
InParanoidⁱ	O15145
KEGG Orthology (KO) More...KOⁱ	K05756
OMAⁱ	PSKWWMS
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0OUM
PhylomeDBⁱ	O15145
TreeFamⁱ	TF314598

Family and domain databases

Gene3Dⁱ	1.10.1760.10, 1 hit
InterProⁱ	View protein in InterPro IPR007204 ARPC3 IPR036753 ARPC3_sf
PANTHERⁱ	PTHR12391 PTHR12391, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04062 P21-Arc, 1 hit
PIRSFⁱ	PIRSF016315 ARP2/3_P21-Arc, 1 hit
SUPFAMⁱ	SSF69060 SSF69060, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

O15145-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MPAYHSSLMD PDTKLIGNMA LLPIRSQFKG PAPRETKDTD IVDEAIYYFK 
        60         70         80         90        100
ANVFFKNYEI KNEADRTLIY ITLYISECLK KLQKCNSKSQ GEKEMYTLGI 
       110        120        130        140        150
TNFPIPGEPG FPLNAIYAKP ANKQEDEVMR AYLQQLRQET GLRLCEKVFD 
       160        170 
PQNDKPSKWW TCFVKRQFMN KSLSGPGQ

Length:178

Mass (Da):20,547

Last modified:January 23, 2007 - v3

Checksum:ⁱ7149F598B48F0EAC

GO

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

C9JZD1	C9JZD1_HUMAN	Actin-related protein 2/3 complex s... Actin-related protein 2/3 complex subunit 3	ARPC3	99	Annotation score:
F8VR50	F8VR50_HUMAN	Actin-related protein 2/3 complex s... Actin-related protein 2/3 complex subunit 3	ARPC3	84	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	134	Q → P in AAB61466 (PubMed:9359840).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF004561 mRNA Translation: AAB61466.1 AF006086 mRNA Translation: AAB64191.1 CR407667 mRNA Translation: CAG28595.1 BC067747 mRNA Translation: AAH67747.1 BC078162 mRNA Translation: AAH78162.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS9146.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001265485.1, NM_001278556.1 NP_001274151.1, NM_001287222.1
UniGeneⁱ	Hs.524741

Genome annotation databases

Ensemblⁱ	ENST00000228825; ENSP00000228825; ENSG00000111229
GeneIDⁱ	10094
KEGGⁱ	hsa:10094
UCSC genome browser More...UCSCⁱ	uc001tqq.5 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O15145	Actin-related protein 2/3 complex subunit 3	)	BOVIN		178	UniRef100_O15145
Actin-related protein 2/3 complex subunit 3		PANPA		158
actin-related protein 2/3 complex subunit 3		TURTR		259
Actin-related protein 2/3 complex subunit 3		MANLE		158
Actin-related protein 2/3 complex subunit 3		CALJA		178
+28

Protein	Similar proteins		Species	Score	Length	Source
O15145	Actin-related protein 2/3 complex subunit 3	)	BOVIN		178	UniRef90_O15145
Actin-related protein 2/3 complex subunit 3		PANPA		158
actin-related protein 2/3 complex subunit 3		TURTR		259
Actin-related protein 2/3 complex subunit 3		MANLE		158
Actin-related protein 2/3 complex subunit 3		CALJA		178
+28

Protein	Similar proteins		Species	Score	Length	Source
O15145	Actin-related protein 2/3 complex subunit 3	)	BOVIN		178	UniRef50_O15145
Actin-related protein 2/3 complex subunit 3		PANPA		158
actin-related protein 2/3 complex subunit 3		TURTR		259
Actin-related protein 2/3 complex subunit 3		MANLE		158
Actin-related protein 2/3 complex subunit 3		CALJA		178
+41

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF004561 mRNA Translation: AAB61466.1 AF006086 mRNA Translation: AAB64191.1 CR407667 mRNA Translation: CAG28595.1 BC067747 mRNA Translation: AAH67747.1 BC078162 mRNA Translation: AAH78162.1
CCDSⁱ	CCDS9146.1
RefSeqⁱ	NP_001265485.1, NM_001278556.1 NP_001274151.1, NM_001287222.1
UniGeneⁱ	Hs.524741

3D structure databases

ProteinModelPortalⁱ	O15145
SMRⁱ	O15145
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115401, 89 interactors
CORUMⁱ	O15145
DIPⁱ	DIP-33187N
IntActⁱ	O15145, 71 interactors
MINTⁱ	O15145
STRINGⁱ	9606.ENSP00000228825

Chemistry databases

DrugBankⁱ	DB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

DrugBankⁱ

DB08235 N-[2-(2-methyl-1H-indol-3-yl)ethyl]thiophene-2-carboxamide

PTM databases

iPTMnetⁱ	O15145
PhosphoSitePlusⁱ	O15145
SwissPalmⁱ	O15145

2D gel databases

OGPⁱ	O15145

OGPⁱ

O15145

Proteomic databases

EPDⁱ	O15145
MaxQBⁱ	O15145
PaxDbⁱ	O15145
PeptideAtlasⁱ	O15145
PRIDEⁱ	O15145
ProteomicsDBⁱ	48470
TopDownProteomicsⁱ	O15145

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	10094
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000228825; ENSP00000228825; ENSG00000111229
GeneIDⁱ	10094
KEGGⁱ	hsa:10094
UCSCⁱ	uc001tqq.5 human

Organism-specific databases

CTDⁱ	10094
DisGeNETⁱ	10094
EuPathDBⁱ	HostDB:ENSG00000111229.15
GeneCardsⁱ	ARPC3
HGNCⁱ	HGNC:706 ARPC3
HPAⁱ	HPA006550
MIMⁱ	604225 gene
neXtProtⁱ	NX_O15145
OpenTargetsⁱ	ENSG00000111229
PharmGKBⁱ	PA25000
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3155 Eukaryota ENOG4111FTG LUCA
GeneTreeⁱ	ENSGT00390000018018
HOGENOMⁱ	HOG000161465
HOVERGENⁱ	HBG050581
InParanoidⁱ	O15145
KOⁱ	K05756
OMAⁱ	PSKWWMS
OrthoDBⁱ	EOG091G0OUM
PhylomeDBⁱ	O15145
TreeFamⁱ	TF314598

Enzyme and pathway databases

Reactomeⁱ	R-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation R-HSA-3928662 EPHB-mediated forward signaling R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs R-HSA-8856828 Clathrin-mediated endocytosis
SIGNORⁱ	O15145

Miscellaneous databases

ChiTaRSⁱ	ARPC3 human
GeneWikiⁱ	ARPC3
GenomeRNAiⁱ	10094
Protein Ontology More...PROⁱ	PR:O15145
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000111229 Expressed in 125 organ(s), highest expression level in leukocyte
ExpressionAtlasⁱ	O15145 baseline and differential
Genevisibleⁱ	O15145 HS

Family and domain databases

Gene3Dⁱ	1.10.1760.10, 1 hit
InterProⁱ	View protein in InterPro IPR007204 ARPC3 IPR036753 ARPC3_sf
PANTHERⁱ	PTHR12391 PTHR12391, 1 hit
Pfamⁱ	View protein in Pfam PF04062 P21-Arc, 1 hit
PIRSFⁱ	PIRSF016315 ARP2/3_P21-Arc, 1 hit
SUPFAMⁱ	SSF69060 SSF69060, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ARPC3_HUMAN
Accessionⁱ	O15145Primary (citable) accession number: O15145 Secondary accession number(s): O00554
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 162 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM

UniProtKB

UniParc

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Supporting data

UniProtKB - O15145 (ARPC3_HUMAN)

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Actin-related protein 2/3 complex subunit 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Cytoskeleton

Other locations

Cytoskeleton

Cytosol

Extracellular region or secreted

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Chemistry databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ