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Protein

1-acyl-sn-glycerol-3-phosphate acyltransferase beta

Gene

AGPAT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.3 Publications

Catalytic activityi

Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.3 Publications

Pathwayi: CDP-diacylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 2, mitochondrial (GPAT2), Glycerol-3-phosphate acyltransferase 3 (GPAT3), Glycerol-3-phosphate acyltransferase 4 (GPAT4), Glycerol-3-phosphate acyltransferase 1, mitochondrial (DKFZp451B1115), Glycerol-3-phosphate acyltransferase 1, mitochondrial, Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM), Glycerol-3-phosphate acyltransferase 1, mitochondrial (GPAM)
  2. 1-acyl-sn-glycerol-3-phosphate acyltransferase alpha (AGPAT1), 1-acyl-sn-glycerol-3-phosphate acyltransferase epsilon (AGPAT5), 1-acyl-sn-glycerol-3-phosphate acyltransferase beta (AGPAT2), 1-acyl-sn-glycerol-3-phosphate acyltransferase gamma (AGPAT3), 1-acyl-sn-glycerol-3-phosphate acyltransferase delta (AGPAT4), Lysocardiolipin acyltransferase 1 (LCLAT1)
  3. Phosphatidate cytidylyltransferase 2 (CDS2), Phosphatidate cytidylyltransferase 1 (CDS1), Phosphatidate cytidylyltransferase, mitochondrial (TAMM41), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase (cdsA), Phosphatidate cytidylyltransferase (CDS1), Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase, Phosphatidate cytidylyltransferase
This subpathway is part of the pathway CDP-diacylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-diacylglycerol from sn-glycerol 3-phosphate, the pathway CDP-diacylglycerol biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • 1-acylglycerol-3-phosphate O-acyltransferase activity Source: BHF-UCL

GO - Biological processi

  • CDP-diacylglycerol biosynthetic process Source: UniProtKB-UniPathway
  • epidermis development Source: Ensembl
  • neutrophil degranulation Source: Reactome
  • phosphatidic acid biosynthetic process Source: BHF-UCL
  • phospholipid metabolic process Source: UniProtKB
  • positive regulation of cytokine-mediated signaling pathway Source: BHF-UCL
  • positive regulation of cytokine production Source: BHF-UCL
  • response to drug Source: Ensembl

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:HS09990-MONOMER
BRENDAi2.3.1.51 2681
ReactomeiR-HSA-1483166 Synthesis of PA
R-HSA-6798695 Neutrophil degranulation
UniPathwayi
UPA00557;UER00613

Chemistry databases

SwissLipidsiSLP:000000096

Names & Taxonomyi

Protein namesi
Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase beta (EC:2.3.1.51)
Alternative name(s):
1-acylglycerol-3-phosphate O-acyltransferase 2
Short name:
1-AGP acyltransferase 2
Short name:
1-AGPAT 2
Lysophosphatidic acid acyltransferase beta
Short name:
LPAAT-beta
Gene namesi
Name:AGPAT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000169692.12
HGNCiHGNC:325 AGPAT2
MIMi603100 gene
neXtProtiNX_O15120

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Transmembranei187 – 207HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Involvement in diseasei

Congenital generalized lipodystrophy 1 (CGL1)2 Publications
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionAn autosomal recessive disorder characterized by a near complete absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
See also OMIM:608594
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017328136G → R in CGL1; reduced 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 PublicationsCorresponds to variant dbSNP:rs797045222EnsemblClinVar.1
Natural variantiVAR_017326140Missing in CGL1; reduced 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 Publications1
Natural variantiVAR_017327228L → P in CGL1; reduced 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 PublicationsCorresponds to variant dbSNP:rs104894100EnsemblClinVar.1
Natural variantiVAR_017325239A → V in CGL1; 90% of wild-type 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 PublicationsCorresponds to variant dbSNP:rs145975461EnsemblClinVar.1

Keywords - Diseasei

Congenital generalized lipodystrophy, Diabetes mellitus, Disease mutation

Organism-specific databases

DisGeNETi10555
GeneReviewsiAGPAT2
MalaCardsiAGPAT2
MIMi608594 phenotype
OpenTargetsiENSG00000169692
Orphaneti528 Berardinelli-Seip congenital lipodystrophy
PharmGKBiPA24622

Chemistry databases

ChEMBLiCHEMBL4772

Polymorphism and mutation databases

BioMutaiAGPAT2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000020819224 – 2781-acyl-sn-glycerol-3-phosphate acyltransferase betaAdd BLAST255

Proteomic databases

EPDiO15120
MaxQBiO15120
PaxDbiO15120
PeptideAtlasiO15120
PRIDEiO15120
ProteomicsDBi48457
48458 [O15120-2]

PTM databases

iPTMnetiO15120
PhosphoSitePlusiO15120

Expressioni

Tissue specificityi

Expressed predominantly in adipose tissue, pancreas and liver.1 Publication

Gene expression databases

BgeeiENSG00000169692 Expressed in 196 organ(s), highest expression level in subcutaneous adipose tissue
CleanExiHS_AGPAT2
ExpressionAtlasiO15120 baseline and differential
GenevisibleiO15120 HS

Organism-specific databases

HPAiHPA019544

Interactioni

Protein-protein interaction databases

BioGridi115806, 13 interactors
CORUMiO15120
IntActiO15120, 7 interactors
STRINGi9606.ENSP00000360761

Chemistry databases

BindingDBiO15120

Structurei

3D structure databases

ProteinModelPortaliO15120
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi98 – 103HXXXXD motif6
Motifi172 – 175EGTR motif4

Domaini

The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2848 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00390000008726
HOVERGENiHBG000676
InParanoidiO15120
KOiK13509
OMAiFKWVSKA
OrthoDBiEOG091G0ICC
PhylomeDBiO15120
TreeFamiTF314867

Family and domain databases

InterProiView protein in InterPro
IPR004552 AGP_acyltrans
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit
TIGRFAMsiTIGR00530 AGP_acyltrn, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O15120-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MELWPCLAAA LLLLLLLVQL SRAAEFYAKV ALYCALCFTV SAVASLVCLL
60 70 80 90 100
RHGGRTVENM SIIGWFVRSF KYFYGLRFEV RDPRRLQEAR PCVIVSNHQS
110 120 130 140 150
ILDMMGLMEV LPERCVQIAK RELLFLGPVG LIMYLGGVFF INRQRSSTAM
160 170 180 190 200
TVMADLGERM VRENLKVWIY PEGTRNDNGD LLPFKKGAFY LAVQAQVPIV
210 220 230 240 250
PVVYSSFSSF YNTKKKFFTS GTVTVQVLEA IPTSGLTAAD VPALVDTCHR
260 270
AMRTTFLHIS KTPQENGATA GSGVQPAQ
Length:278
Mass (Da):30,914
Last modified:January 1, 1998 - v1
Checksum:i1E58F537F703BE9F
GO
Isoform 2 (identifier: O15120-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     165-196: Missing.

Note: No experimental confirmation available.
Show »
Length:246
Mass (Da):27,279
Checksum:i2A6E877F48DC3FE9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126L → V in AAB64299 (PubMed:9291118).Curated1
Sequence conflicti200V → F in AAH00026 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_017328136G → R in CGL1; reduced 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 PublicationsCorresponds to variant dbSNP:rs797045222EnsemblClinVar.1
Natural variantiVAR_017326140Missing in CGL1; reduced 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 Publications1
Natural variantiVAR_017327228L → P in CGL1; reduced 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 PublicationsCorresponds to variant dbSNP:rs104894100EnsemblClinVar.1
Natural variantiVAR_017325239A → V in CGL1; 90% of wild-type 1-acyl-sn-glycerol-3-phosphate acyltransferase activity. 2 PublicationsCorresponds to variant dbSNP:rs145975461EnsemblClinVar.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005071165 – 196Missing in isoform 2. 1 PublicationAdd BLAST32

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000237 mRNA Translation: AAC51649.1
AF011374 mRNA Translation: AAB64299.1
U56418 mRNA Translation: AAB58776.2
AL590226 Genomic DNA No translation available.
CH471090 Genomic DNA Translation: EAW88249.1
CH471090 Genomic DNA Translation: EAW88251.1
BC000026 mRNA Translation: AAH00026.1
BC004529 mRNA Translation: AAH04529.1
CCDSiCCDS35181.1 [O15120-2]
CCDS7003.1 [O15120-1]
RefSeqiNP_001012745.1, NM_001012727.1 [O15120-2]
NP_006403.2, NM_006412.3 [O15120-1]
UniGeneiHs.320151

Genome annotation databases

EnsembliENST00000371694; ENSP00000360759; ENSG00000169692 [O15120-2]
ENST00000371696; ENSP00000360761; ENSG00000169692 [O15120-1]
ENST00000538402; ENSP00000438919; ENSG00000169692 [O15120-1]
GeneIDi10555
KEGGihsa:10555
UCSCiuc004cii.2 human [O15120-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000237 mRNA Translation: AAC51649.1
AF011374 mRNA Translation: AAB64299.1
U56418 mRNA Translation: AAB58776.2
AL590226 Genomic DNA No translation available.
CH471090 Genomic DNA Translation: EAW88249.1
CH471090 Genomic DNA Translation: EAW88251.1
BC000026 mRNA Translation: AAH00026.1
BC004529 mRNA Translation: AAH04529.1
CCDSiCCDS35181.1 [O15120-2]
CCDS7003.1 [O15120-1]
RefSeqiNP_001012745.1, NM_001012727.1 [O15120-2]
NP_006403.2, NM_006412.3 [O15120-1]
UniGeneiHs.320151

3D structure databases

ProteinModelPortaliO15120
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115806, 13 interactors
CORUMiO15120
IntActiO15120, 7 interactors
STRINGi9606.ENSP00000360761

Chemistry databases

BindingDBiO15120
ChEMBLiCHEMBL4772
SwissLipidsiSLP:000000096

PTM databases

iPTMnetiO15120
PhosphoSitePlusiO15120

Polymorphism and mutation databases

BioMutaiAGPAT2

Proteomic databases

EPDiO15120
MaxQBiO15120
PaxDbiO15120
PeptideAtlasiO15120
PRIDEiO15120
ProteomicsDBi48457
48458 [O15120-2]

Protocols and materials databases

DNASUi10555
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371694; ENSP00000360759; ENSG00000169692 [O15120-2]
ENST00000371696; ENSP00000360761; ENSG00000169692 [O15120-1]
ENST00000538402; ENSP00000438919; ENSG00000169692 [O15120-1]
GeneIDi10555
KEGGihsa:10555
UCSCiuc004cii.2 human [O15120-1]

Organism-specific databases

CTDi10555
DisGeNETi10555
EuPathDBiHostDB:ENSG00000169692.12
GeneCardsiAGPAT2
GeneReviewsiAGPAT2
HGNCiHGNC:325 AGPAT2
HPAiHPA019544
MalaCardsiAGPAT2
MIMi603100 gene
608594 phenotype
neXtProtiNX_O15120
OpenTargetsiENSG00000169692
Orphaneti528 Berardinelli-Seip congenital lipodystrophy
PharmGKBiPA24622
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2848 Eukaryota
COG0204 LUCA
GeneTreeiENSGT00390000008726
HOVERGENiHBG000676
InParanoidiO15120
KOiK13509
OMAiFKWVSKA
OrthoDBiEOG091G0ICC
PhylomeDBiO15120
TreeFamiTF314867

Enzyme and pathway databases

UniPathwayi
UPA00557;UER00613

BioCyciMetaCyc:HS09990-MONOMER
BRENDAi2.3.1.51 2681
ReactomeiR-HSA-1483166 Synthesis of PA
R-HSA-6798695 Neutrophil degranulation

Miscellaneous databases

GeneWikiiAGPAT2
GenomeRNAii10555
PROiPR:O15120
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169692 Expressed in 196 organ(s), highest expression level in subcutaneous adipose tissue
CleanExiHS_AGPAT2
ExpressionAtlasiO15120 baseline and differential
GenevisibleiO15120 HS

Family and domain databases

InterProiView protein in InterPro
IPR004552 AGP_acyltrans
IPR002123 Plipid/glycerol_acylTrfase
PfamiView protein in Pfam
PF01553 Acyltransferase, 1 hit
SMARTiView protein in SMART
SM00563 PlsC, 1 hit
TIGRFAMsiTIGR00530 AGP_acyltrn, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPLCB_HUMAN
AccessioniPrimary (citable) accession number: O15120
Secondary accession number(s): O00516
, O15106, Q5VUD3, Q5VUD4, Q9BSV7, Q9BWR7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 170 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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