UniProtKB - O15084 (ANR28_HUMAN)
Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A
ANKRD28
Functioni
Putative regulatory subunit of protein phosphatase 6 (PP6) that may be involved in the recognition of phosphoprotein substrates. Involved in the PP6-mediated dephosphorylation of NFKBIE opposing its degradation in response to TNF-alpha. Selectively inhibits the phosphatase activity of PPP1C. Targets PPP1C to modulate HNRPK phosphorylation.
2 PublicationsEnzyme and pathway databases
PathwayCommonsi | O15084 |
Reactomei | R-HSA-171319, Telomere Extension By Telomerase R-HSA-204005, COPII-mediated vesicle transport |
SignaLinki | O15084 |
SIGNORi | O15084 |
Names & Taxonomyi
Protein namesi | Recommended name: Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit AShort name: PP6-ARS-A Short name: Serine/threonine-protein phosphatase 6 regulatory subunit ARS-A Alternative name(s): Ankyrin repeat domain-containing protein 28 Phosphatase interactor targeting protein hnRNP K Short name: PITK |
Gene namesi | Name:ANKRD28 Synonyms:KIAA0379 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29024, ANKRD28 |
MIMi | 611122, gene |
neXtProti | NX_O15084 |
VEuPathDBi | HostDB:ENSG00000206560 |
Subcellular locationi
Nucleus
- nucleoplasm 1 Publication
Note: Seems to be excluded from nucleoli.
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1007 – 1011 | SKTVS → AKTVA: Marked decrease in phosphorylation. Increased PPP1C-binding. No effect on HNRPK-binding. 1 Publication | 5 |
Organism-specific databases
DisGeNETi | 23243 |
OpenTargetsi | ENSG00000206560 |
PharmGKBi | PA134880251 |
Miscellaneous databases
Pharosi | O15084, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4105921 |
Genetic variation databases
BioMutai | ANKRD28 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066919 | 1 – 1053 | Serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit AAdd BLAST | 1053 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1007 | Phosphoserine1 Publication | 1 | |
Modified residuei | 1011 | PhosphoserineCombined sources1 Publication | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O15084 |
jPOSTi | O15084 |
MassIVEi | O15084 |
MaxQBi | O15084 |
PaxDbi | O15084 |
PeptideAtlasi | O15084 |
PRIDEi | O15084 |
ProteomicsDBi | 48437 [O15084-3] 48438 [O15084-1] 48439 [O15084-2] 48440 [O15084-4] |
PTM databases
GlyGeni | O15084, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O15084 |
PhosphoSitePlusi | O15084 |
Expressioni
Gene expression databases
Bgeei | ENSG00000206560, Expressed in caput epididymis and 237 other tissues |
ExpressionAtlasi | O15084, baseline and differential |
Genevisiblei | O15084, HS |
Organism-specific databases
HPAi | ENSG00000206560, Low tissue specificity |
Interactioni
Subunit structurei
Protein phosphatase 6 (PP6) holoenzyme is proposed to be a heterotrimeric complex formed by the catalytic subunit, a SAPS domain-containing subunit (PP6R) and an ankyrin repeat-domain containing regulatory subunit (ARS).
Interacts with PPP1C and HNRPK.
Interacts with PPP6C, PPP6R1 and PPP6R3.
2 PublicationsBinary interactionsi
O15084
Protein-protein interaction databases
BioGRIDi | 116847, 197 interactors |
CORUMi | O15084 |
DIPi | DIP-27583N |
IntActi | O15084, 119 interactors |
MINTi | O15084 |
STRINGi | 9606.ENSP00000382379 |
Miscellaneous databases
RNActi | O15084, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 40 – 69 | ANK 1Add BLAST | 30 | |
Repeati | 73 – 102 | ANK 2Add BLAST | 30 | |
Repeati | 106 – 135 | ANK 3Add BLAST | 30 | |
Repeati | 139 – 168 | ANK 4Add BLAST | 30 | |
Repeati | 172 – 201 | ANK 5Add BLAST | 30 | |
Repeati | 205 – 234 | ANK 6Add BLAST | 30 | |
Repeati | 238 – 267 | ANK 7Add BLAST | 30 | |
Repeati | 271 – 301 | ANK 8Add BLAST | 31 | |
Repeati | 305 – 334 | ANK 9Add BLAST | 30 | |
Repeati | 338 – 367 | ANK 10Add BLAST | 30 | |
Repeati | 371 – 400 | ANK 11Add BLAST | 30 | |
Repeati | 404 – 433 | ANK 12Add BLAST | 30 | |
Repeati | 437 – 466 | ANK 13Add BLAST | 30 | |
Repeati | 470 – 500 | ANK 14Add BLAST | 31 | |
Repeati | 504 – 534 | ANK 15Add BLAST | 31 | |
Repeati | 549 – 578 | ANK 16Add BLAST | 30 | |
Repeati | 582 – 611 | ANK 17Add BLAST | 30 | |
Repeati | 616 – 645 | ANK 18Add BLAST | 30 | |
Repeati | 652 – 681 | ANK 19Add BLAST | 30 | |
Repeati | 685 – 714 | ANK 20Add BLAST | 30 | |
Repeati | 718 – 747 | ANK 21Add BLAST | 30 | |
Repeati | 755 – 784 | ANK 22Add BLAST | 30 | |
Repeati | 787 – 817 | ANK 23Add BLAST | 31 | |
Repeati | 822 – 851 | ANK 24Add BLAST | 30 | |
Repeati | 855 – 885 | ANK 25Add BLAST | 31 | |
Repeati | 889 – 918 | ANK 26Add BLAST | 30 | |
Repeati | 925 – 954 | ANK 27Add BLAST | 30 |
Keywords - Domaini
ANK repeat, RepeatPhylogenomic databases
eggNOGi | KOG0504, Eukaryota |
GeneTreei | ENSGT00950000182908 |
HOGENOMi | CLU_000134_58_0_1 |
InParanoidi | O15084 |
OMAi | YNGHLEM |
OrthoDBi | 1115202at2759 |
TreeFami | TF312824 |
Family and domain databases
Gene3Di | 1.25.40.20, 12 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf |
Pfami | View protein in Pfam PF00023, Ank, 3 hits PF12796, Ank_2, 8 hits PF13637, Ank_4, 2 hits |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 28 hits |
SUPFAMi | SSF48403, SSF48403, 4 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 24 hits |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative promoter usage and alternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAFLKLRDQP SLVQAIFNGD PDEVRALIFK KEDVNFQDNE KRTPLHAAAY
60 70 80 90 100
LGDAEIIELL ILSGARVNAK DSKWLTPLHR AVASCSEEAV QVLLKHSADV
110 120 130 140 150
NARDKNWQTP LHIAAANKAV KCAEALVPLL SNVNVSDRAG RTALHHAAFS
160 170 180 190 200
GHGEMVKLLL SRGANINAFD KKDRRAIHWA AYMGHIEVVK LLVSHGAEVT
210 220 230 240 250
CKDKKSYTPL HAAASSGMIS VVKYLLDLGV DMNEPNAYGN TPLHVACYNG
260 270 280 290 300
QDVVVNELID CGAIVNQKNE KGFTPLHFAA ASTHGALCLE LLVGNGADVN
310 320 330 340 350
MKSKDGKTPL HMTALHGRFS RSQTIIQSGA VIDCEDKNGN TPLHIAARYG
360 370 380 390 400
HELLINTLIT SGADTAKRGI HGMFPLHLAA LSGFSDCCRK LLSSGFDIDT
410 420 430 440 450
PDDFGRTCLH AAAAGGNLEC LNLLLNTGAD FNKKDKFGRS PLHYAAANCN
460 470 480 490 500
YQCLFALVGS GASVNDLDER GCTPLHYAAT SDTDGKCLEY LLRNDANPGI
510 520 530 540 550
RDKQGYNAVH YSAAYGHRLC LQLIASETPL DVLMETSGTD MLSDSDNRAT
560 570 580 590 600
ISPLHLAAYH GHHQALEVLV QSLLDLDVRN SSGRTPLDLA AFKGHVECVD
610 620 630 640 650
VLINQGASIL VKDYILKRTP IHAAATNGHS ECLRLLIGNA EPQNAVDIQD
660 670 680 690 700
GNGQTPLMLS VLNGHTDCVY SLLNKGANVD AKDKWGRTAL HRGAVTGHEE
710 720 730 740 750
CVDALLQHGA KCLLRDSRGR TPIHLSAACG HIGVLGALLQ SAASMDANPA
760 770 780 790 800
TADNHGYTAL HWACYNGHET CVELLLEQEV FQKTEGNAFS PLHCAVINDN
810 820 830 840 850
EGAAEMLIDT LGASIVNATD SKGRTPLHAA AFTDHVECLQ LLLSHNAQVN
860 870 880 890 900
SVDSTGKTPL MMAAENGQTN TVEMLVSSAS AELTLQDNSK NTALHLACSK
910 920 930 940 950
GHETSALLIL EKITDRNLIN ATNAALQTPL HVAARNGLTM VVQELLGKGA
960 970 980 990 1000
SVLAVDENGY TPALACAPNK DVADCLALIL ATMMPVSSSS PLSSLTFNAI
1010 1020 1030 1040 1050
NRYTNTSKTV SFEALPIMRN EPSSYCSFNN IGGEQEYLYT DVDELNDSDS
ETY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB4DIW9 | B4DIW9_HUMAN | Serine/threonine-protein phosphatas... | ANKRD28 | 722 | Annotation score: | ||
F8WDU2 | F8WDU2_HUMAN | Serine/threonine-protein phosphatas... | ANKRD28 | 14 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 293 | V → A in AAQ72374 (Ref. 1) Curated | 1 | |
Sequence conflicti | 500 | I → V in AAQ72374 (Ref. 1) Curated | 1 | |
Sequence conflicti | 500 | I → V in BAC86737 (PubMed:14702039).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_012433 | 1 – 154 | Missing in isoform 2. 2 PublicationsAdd BLAST | 154 | |
Alternative sequenceiVSP_041013 | 1 – 9 | MAFLKLRDQ → MSRVCIVVLEEVEDESPAFI SKLPQENKSLHSPPSGNVLV RY in isoform 3. 2 Publications | 9 | |
Alternative sequenceiVSP_041014 | 1 – 9 | MAFLKLRDQ → MSRVCIVVLEEVEDESPAFI SKLPQENKSLHSPPSGNVL in isoform 4. 1 Publication | 9 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY367056 mRNA Translation: AAQ72374.1 Frameshift. AB002377 mRNA Translation: BAA20833.2 Different initiation. AK126888 mRNA Translation: BAC86737.1 Sequence problems. AK293770 mRNA Translation: BAG57186.1 BC106948 mRNA Translation: AAI06949.2 BC113868 mRNA Translation: AAI13869.1 BC114476 mRNA Translation: AAI14477.1 |
CCDSi | CCDS46769.1 [O15084-3] CCDS74908.1 [O15084-2] |
RefSeqi | NP_001182027.1, NM_001195098.1 [O15084-2] NP_001182028.1, NM_001195099.1 [O15084-2] NP_056014.2, NM_015199.3 [O15084-3] XP_005265053.1, XM_005264996.3 XP_011531842.1, XM_011533540.2 XP_016861515.1, XM_017006026.1 XP_016861516.1, XM_017006027.1 |
Genome annotation databases
Ensembli | ENST00000399451; ENSP00000382379; ENSG00000206560 ENST00000412318; ENSP00000397341; ENSG00000206560 ENST00000624145; ENSP00000485421; ENSG00000206560 [O15084-2] ENST00000683139; ENSP00000508086; ENSG00000206560 [O15084-4] |
GeneIDi | 23243 |
KEGGi | hsa:23243 |
MANE-Selecti | ENST00000683139.1; ENSP00000508086.1; NM_001349278.2; NP_001336207.1 [O15084-4] |
UCSCi | uc003cai.2, human [O15084-3] |
Keywords - Coding sequence diversityi
Alternative promoter usage, Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY367056 mRNA Translation: AAQ72374.1 Frameshift. AB002377 mRNA Translation: BAA20833.2 Different initiation. AK126888 mRNA Translation: BAC86737.1 Sequence problems. AK293770 mRNA Translation: BAG57186.1 BC106948 mRNA Translation: AAI06949.2 BC113868 mRNA Translation: AAI13869.1 BC114476 mRNA Translation: AAI14477.1 |
CCDSi | CCDS46769.1 [O15084-3] CCDS74908.1 [O15084-2] |
RefSeqi | NP_001182027.1, NM_001195098.1 [O15084-2] NP_001182028.1, NM_001195099.1 [O15084-2] NP_056014.2, NM_015199.3 [O15084-3] XP_005265053.1, XM_005264996.3 XP_011531842.1, XM_011533540.2 XP_016861515.1, XM_017006026.1 XP_016861516.1, XM_017006027.1 |
3D structure databases
SMRi | O15084 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116847, 197 interactors |
CORUMi | O15084 |
DIPi | DIP-27583N |
IntActi | O15084, 119 interactors |
MINTi | O15084 |
STRINGi | 9606.ENSP00000382379 |
Chemistry databases
ChEMBLi | CHEMBL4105921 |
PTM databases
GlyGeni | O15084, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O15084 |
PhosphoSitePlusi | O15084 |
Genetic variation databases
BioMutai | ANKRD28 |
Proteomic databases
EPDi | O15084 |
jPOSTi | O15084 |
MassIVEi | O15084 |
MaxQBi | O15084 |
PaxDbi | O15084 |
PeptideAtlasi | O15084 |
PRIDEi | O15084 |
ProteomicsDBi | 48437 [O15084-3] 48438 [O15084-1] 48439 [O15084-2] 48440 [O15084-4] |
Protocols and materials databases
Antibodypediai | 26857, 107 antibodies from 21 providers |
DNASUi | 23243 |
Genome annotation databases
Ensembli | ENST00000399451; ENSP00000382379; ENSG00000206560 ENST00000412318; ENSP00000397341; ENSG00000206560 ENST00000624145; ENSP00000485421; ENSG00000206560 [O15084-2] ENST00000683139; ENSP00000508086; ENSG00000206560 [O15084-4] |
GeneIDi | 23243 |
KEGGi | hsa:23243 |
MANE-Selecti | ENST00000683139.1; ENSP00000508086.1; NM_001349278.2; NP_001336207.1 [O15084-4] |
UCSCi | uc003cai.2, human [O15084-3] |
Organism-specific databases
CTDi | 23243 |
DisGeNETi | 23243 |
GeneCardsi | ANKRD28 |
HGNCi | HGNC:29024, ANKRD28 |
HPAi | ENSG00000206560, Low tissue specificity |
MIMi | 611122, gene |
neXtProti | NX_O15084 |
OpenTargetsi | ENSG00000206560 |
PharmGKBi | PA134880251 |
VEuPathDBi | HostDB:ENSG00000206560 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0504, Eukaryota |
GeneTreei | ENSGT00950000182908 |
HOGENOMi | CLU_000134_58_0_1 |
InParanoidi | O15084 |
OMAi | YNGHLEM |
OrthoDBi | 1115202at2759 |
TreeFami | TF312824 |
Enzyme and pathway databases
PathwayCommonsi | O15084 |
Reactomei | R-HSA-171319, Telomere Extension By Telomerase R-HSA-204005, COPII-mediated vesicle transport |
SignaLinki | O15084 |
SIGNORi | O15084 |
Miscellaneous databases
BioGRID-ORCSi | 23243, 10 hits in 1053 CRISPR screens |
ChiTaRSi | ANKRD28, human |
GenomeRNAii | 23243 |
Pharosi | O15084, Tbio |
PROi | PR:O15084 |
RNActi | O15084, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000206560, Expressed in caput epididymis and 237 other tissues |
ExpressionAtlasi | O15084, baseline and differential |
Genevisiblei | O15084, HS |
Family and domain databases
Gene3Di | 1.25.40.20, 12 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR036770, Ankyrin_rpt-contain_sf |
Pfami | View protein in Pfam PF00023, Ank, 3 hits PF12796, Ank_2, 8 hits PF13637, Ank_4, 2 hits |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 28 hits |
SUPFAMi | SSF48403, SSF48403, 4 hits |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 24 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | ANR28_HUMAN | |
Accessioni | O15084Primary (citable) accession number: O15084 Secondary accession number(s): B4DES5 Q6ZT57 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | May 3, 2011 | |
Last modified: | February 23, 2022 | |
This is version 191 of the entry and version 5 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot